Good morning ladies and gentlemen. My name is Hidayatullah arief, with dr.

Marindra and
dr. Dini as supervisor, we are from Faculty of medicine, Islamic university of malang,
Indonesia
In this occasion, I would like to present my research about the effect of combined imperata
cylindrica, gynura procumbens, and eugenia polyantha on density of aortic collagen in
hypertensive rats model

Ok now I’ll explain about my research

Hypertension is a seriuos health problem.
Based on World Health Organization, more than 40% of world population is suffering from
hypertension.
In Indonesia, hypertension prevalence on people aged more than 18 years old is 25,8%.

Hipertensi lama kelamaan akan terjadi perubahan hemodinamik yang menyebabkan shear
stres dan menyebabkan stres oksidatif yang menyebabkan peningkatan ROS dan
menyebabkan disfungsi endotel yang terjadi pada lapisan tunika media yaitu jaringan
kolagen. Sehingga terjadi proliferasai kolagen yang merupakan indikator lesi vaskuler
karena HT dan dapat menyebabkan komplikasi dalam waktu lama
Prolonged hypertension can lead to hemodynamical change which can cause increased
shear stress and also causing oxidative stress causing increased ROS and also causing
endothelial disfunction occuring on tunica media layer which is collagen. Thus, causing
collagen proliferation which is vascular lession indicator caused by hypertension that can
cause long-term complication.

Indonesia has many herbs and these several herbs as for antihypertension, remedy, which
are Imperata cylindrica, Gynura procumbens, and Eugenia polyantha
Imperata cylindrica, Gynura procumbens, and Eugenia polyantha contains secondary
metabolic that’s could potentially be used as an antioxidant and antihypertensive
With that reason above, I’ve done a research to prove the effect of combined Imperata
cylindrica, Gynura procumbens, and Eugenia polyantha as antioxidant to increase SOD
level and decrease MDA level of heart.
This method is experimental laboratory research with control group post-test only design
and use 20 (twenty) male wistar rats which divided into 5 groups, which are NC, PC, T1,
T2 and T3.
Hypertension in rats is induced by DOCA 10 mg/kgBW subcutaneous injection and NaCl
1% ad libitum for 9 weeks, however NC is excluded.
And for IGE decoction, it’s given in 3 different doses. 12,6 mg/rat dosage for T1, 25,2
mg/rat dosage for T2 and 50,4 mg/rat dosage for T3 as long as 5 weeks after being
induced by DOCA-NaCl for 4 weeks.
The data collected were then analyzed using one way anova.

This is the result from this research.

Pada diagram ini terlihat bahwa pada kontrol + terjadinya peningkatan kepaatan kolagen
yang diinduksi DOCA-NaCl dibandingkan dengan kontrol negatif. Sedangkan pada semua
kelompok perlakuan terdapat penurunan kepadatan kolagen stelah diinduksi IGE,
khususnya pada P2 dn P3 yang tidak berbeda signfikan dengan kontrol negatif.
From the diagram, we can see increased collagen density on positive control group
induced by DOCA-NaCl compared with negative control group. Meanwhile, collagen
density was shown to be reduced on every treatment group after induced by IGE,
especially on experiment group 2 and 3 which are not significantly different from negative
control group.

Dapat kita lihat pada gambaran preparat setelah dilakukan pewarnaan Masson Trichome
dan pembacaan hasil dengan image-J terlihat pada kontrol negatif kolagen yang berwarna
biru lebih sedikit dari pada kontrol positif. Dan pada kelompok perlakuan terlihat bahwa
pada P2 dan P3 kolagennya hampir sama dengan kelompok kontrol negatif.
The sample was colored with Masson Trichome and observed with image-J software. We
can see that on negative control group, blue colorred collagen appeared to be in lesser
density compared to positive control. While on treatment group 2 and 3 was shown that
the collagen density is not significantly different compared with negative control group.

And the conclusion is............................

Ok that’s all of my presentation

I would like to thanks ICAMBBE for this great opportunity
Also thanks for all my supervisor in faculty of medicine, islamic university of malang
This project is financially support by IOM medical faculty islamic university of malang