Documente Academic
Documente Profesional
Documente Cultură
ISSN: 2277–4998
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Sadeghi Limanjoob R et al Research Article
samples were chosen randomly. After spinal cord injury, biometry and then necropsy was
performed to obtain a sample of kidney tissue. All samples preserved in 4% formalin and
then transferred to the pathology laboratory of Veterinary Medicine and acting opioid
preparation of pathology slides and staining Hematocalcilin - eosin. The sections were
examined pathologically. Among the samples, there were no nephrocalcinosis symptoms but
a degree of complicated pathology of renal tubular dilation and hyaline casts, acute tubular
necrosis and renal pathology was observed to increase melanin in some sections. According
to this fact that amount of deaths were low, regarding transition coefficient of nutrition in
fishes' food had depletion, the corps of fish were of low quality. Therefore further research is
recommended .
Keywords: Nephrocalcinosis, Histopathologic, Trout, Rainbow, Mamasani
INTRODUCTION
Today the fish specially types of protein symptoms and investigating disease losses
such as salmon specify an important share such as kidney, cyst and urinal tubes. The
of economic and animal protein sources in kidney has two role of blood filtration (urine
lots of countries. Rainbow salmon is rapidly production), and is responsible for making
developing all over Iran too. Untold science blood. It has a long dark structure on the
and research, and more research is needed. top of abdominal cavity which is stuck to
The rainbow trout salmo garidneri spine and is continued to the rectum. There
perfection is more to be named because of is a vein crossing through the kidney. Thin-
its morphological features of intact and walled bladder is the organ that is located
separated by sex and continuous above the anus. The last member of the
anchoryncous called O. mykiss. The name excretory system is urinary tract and
means the male's nose is HOOKED. The administrative openings [5-8].
growth of a specific member of a hook on Successful breeding of rainbow trout
the snout of the fish breeding season, and requires serious care and management is
like is created. Duration of different life desirable. It's important to understand the
stages of Pacific salmon, Atlantic salmon biological needs of fish depending on
are even more stable situation [1-4]. Rainbow trout animal consciousness.
Given the direct connection between Education Management should always
symptoms and pathologic losses caused by consider this in your calculations. So you
diseases and different organs, knowing should always take notes on the breeding of
organs can contribute to knowing clinic rainbow trout kept in mind. Fish should be
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organs. After that we open the fish body to After investigating pathologic slides we
see all its inner organs. Kidney is at the found no sign of nephrocalcinosis in
lower level of the spin of Fish and sticking pathologic sections which appear like
to it and pulled the dark part is visible. Then basophilic deposits in colors of
with a plastic spoon we cut it and put it in a hematocalcilin-eosin. But same degrees of
container of 4% formalin. In the present edema of one core cells, necrosis of urinary
study 75 samples tissues of 2*2 cm were tract, hyaline cyst inside the urinary tract
collected from all parts of field pools. and increased melanin pigment in the
Tissue samples were sent to laboratory of interstitial space were observed in some
Veterinary Medicine Kazeroon to provide sections. In addition, in measurements made
histopathology sections. in the field ph of water were approximately
RESULTS 7-5/6 and water temperatures between 16-18
have been variable.
Ca
Necrosis
Melanization
Inflamition
x
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DISCUSSION AND CONCLUSIONS fresh water with high carbon dioxide can be
In recent years along with rapid growth of reduced feed conversion correlation and
aquatic industry, need for high number of growth. Also when fish with high levels of
infants has caused obvious increase in fish carbon dioxide will exposed for a long time,
density during maintenance in growth Carbon dioxide levels is risen (hypercepnia)
containers. This subject was achievable by and pH levels can also result in reduced
increasing the oxygen in the water which respiratory acidosis and the fish trying to
increases the density of carbon dioxide compensate acidosis with elevating levels of
when the current is decreased and is caused plasma bicarbonate and phosphate excretion
carbon dioxide and ammoniac to increase. through the kidneys (nephrocalcinosis). In
Long-term effects of contact between 2001 [10] in US observed a case of severe
rainbow trout and Smoltz of Atlantic salmon nephrocalcinosis in a tilapia fish suffering
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from this disease which was sent to a aquatic growth to maintain alkali feature of
growth system lab to diagnose the causes of water up to 4 weeks which by stopping the
death. Losses such as dilated ureter, and use of sodium carbonate and replacing it
renal mineral deposits inside pipes, forming with sodium bicarbonate death and causes
cysts in tubular kidney, ureter epithelium of nephrocalcinosis were decreased.
degeneration was also observed in the Although this study also found no calcium
granuloma forms of the same deposits in the renal, tubular necrosis was
histopathological findings in observed in some sections. It seems that
nephrocalcinosis Atlantic halibut larvae. In these observations may indicate early a
the present study, however we found no stage of the primary pathology is a
trace of the calcified deposits calcium, nephrocalcinosis.
hyaline cysts in the urine tubes were [11] during investigations on
observed .The mineral deposits in the renal nephrocalcinosis between rainbow salmons
tubular and interstitial tissue, as there were on effects of co2 density, they put salmons
frequent. Capitolare kidney necrosis was in water with excess amounts of co2, 12mg/l
observed in most of the tested tilapia. There to 60mg/l for 275 days that during this time
was also an increase in the number of cells there was symptoms of nephrocalcinosis.
in the kidney glomerulus, the only obvious Histology and chemical invest igations
change observed were mild lipid changes showed that the outbreak and severity of
and hepatocytes. nephrocalcinosis increases as the density of
Hyperemia of the kidney tubes were CO2 increases. Also [12] have studies on
observed in examined fish. During blood long term lethal effects of carbon dioxide on
factors investigations (sodium, bicarbonate, Atlantic salmon Smoltz on: Adjustable
calcium, phosphate and total protein) that Ionic, hematology, element composition,
this fish was conducted over several nephrocalcinosis and growth parameters.
months, the most obvious changes in plasma Significant difference were observed
bicarbonate was included. In a way that the between the groups in terms of the average
levels of sampled bicarbonate during last concentration of hemoglobin, hematocrit, or
month before are sending to lab were about activity of Na + T-k +-ATP gill
8 MEq/1. While 3 months later levels of nephrocalcinosis and the content of calcium
bicarbonate reached to 26 mEq/1. Important in the kidneys in response to rising levels of
data from the history of sample was using carbon dioxide in water at day 58 was
calcium carbonate to common range in observed in the examined fish 1 Once in the
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