KeenView en

The
telePresence
Add-in
KeenView Camera
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About this document
This document describes the procedure for installing the KeenView
camera on a TEM column.
It includes the following:
• the installation of hardware components on the PC running your
image analysis program;
• cabling;
• proper set up of the camera for regular use;
• and some technical data.
Please read these instructions before performing an installation.
Some parts or procedures may differ for different models of micro-
scope, but this should not cause any major problems.
KeenView User´s Guide Software Control of the KeenView camera 3
ACQUIRING IMAGES ...............................................................3

Predefined input channels ...................................................3
DEFINING OFFSET AND GAIN CORRECTION ......................5
How to acquire reference images .............................7
How to switch shading correction on ........................9
How to check the shading correction images .........10
How to adjust the exposure time ............................11
How to check reference images .............................11
ACQUIRING IMAGES USING INTX .......................................12
The intelligent Exposure dialog box ...................................14
CALIBRATING INPUT CHANNEL ..........................................16
USING THE RTFFT ................................................................18
ACTIVATING THE REMOTE CONTROL ................................20
USING THE MACRO TAB IN THE INPUT CHANNEL ............20
THE CAMERA CONTROL DIALOG BOX ...............................21
CONFIGURING INPUT CHANNEL .........................................25
The Configure Input dialog box ..........................................25
Quick-start Installation 33
Camera Installation Procedure 34
Software Installation under Windows XP 35

KeenView0806E
Appendix 46
Technical specifications .....................................................46

Packing List .......................................................................46
Software Control of the KeenView camera
KeenView
Software Control of the KeenView camera

Your image analysis software uses the concept of logical input channels for
the acquisition of images. A logical input channel encompasses the con-
figuration of the device being used as well as other useful attributes and
methods which describe the acquisition and pre-processing of an image.
At any given time there can be only one input channel can be active. The
name of this active channel is displayed in the status bar.
Acquiring images
During the software installation three input channels will be installed for the
KeenView. It is assumed that you will use such a predefined input channel
when you follow the step-by-step-instructions set out here.
Predefined input channels

After installation you will have two predefined input channels at your dis-
posal: KeenView Search and KeenView Snapshot.
KeenView Search The input channel KeenView Search is well suited for working through the
specimen and searching for points of interest. It uses binning to ensure a
high frame rate. The online histogram is activated as well.
KeenView Snapshot The input channel KeenView Snapshot is well suited for the acquisition of
high quality snapshots. Binning is switched off in order to make use of the
camera’s full resolution capabilities.The online histogram is activated to
enable you to set the optimum exposure time.
How a new input channel is created and configured is described on page 25.
Selecting the KeenView 1) Switch on your TEM.
input channel 2) Start your image analysis program.
3) Remove the fluorescence screen.
4) Select the Image > Set Input... command.
3
" The Set Input dialog box will be opened. It shows your KeenView
camera’s predefined input channels.
5) Select the channel named KeenView Search.
The Set Input dialog box
contains two predefined
channels.
KeenView • Acquiring images
Scanning the specimen

Switching to the 1) Click the Acquire button located in the Standard button bar.
live mode " The camera’s live image will be displayed in the image window.
" The Histogram dialog box will be opened, showing the histogram of
the live image. The histogram will be continuously updated.
2) Select the Image > Camera Control... command.

" The Camera Control dialog box will be opened.
3) Change the exposure time, if necessary. Click the Auto button in the
Camera Control dialog box to have the exposure time calculated auto-
matically.
Camera Control
4) Locate the sample position you are interested in.

5) Use the microscope control to focus the image.
Stopping the live 6) Click the Acquire button in the Standard button bar.
mode " The live image will be stopped.
4
" The Histogram dialog box will be closed.

Switching to 7) Select the Image > Set Input command.
snapshot " The Set Input dialog box will be opened. It shows your KeenView
mode camera’s three predefined input channels.
8) Select the channel named KeenView Snapshot.
Acquiring the image
Switching to the 9) Click the Acquire button located in the Standard button bar.
live mode " The camera’s live image will be displayed in the image window.
" The Histogram dialog box will be opened, showing the histogram of
the live image. The histogram will be continuously updated.
11) Use the microscope control to focus the image.
" Make sure you achieve the optimum focus now.
12) Change the exposure time, if necessary. Click the Auto button in the
Camera Control dialog box to have the exposure time calculated auto-
matically.
Defining offset and gain correction
Acquiring an 13) Click the Snapshot button located in the Standard button bar.
image " The live image will be frozen.
Saving an image 14) Use the File > Save As... command to save the image file, or insert the
image into an image database. You should use the TIF file format in or-
der to save all additional image information.
If you want to acquire more images of the same specimen, you don’t have
to switch back to the input channel KeenView Search. Just start again with
step 9 of the preceding list.

Shading correction is required for the acquisition of best quality images. Two
reference images (one offset image and one gain image) are required for
each format used in the input channels for the purpose of automatic shading
correction. In fact, you will mainly use the shading correction in the snapshot
mode, i.e., at high resolution, for shading correction reduces the speed in
the live mode. But you also need it when you focus your camera.
Online shading By using the Online shading correction your image analysis program cor-
correction rects fixed image structures (resulting from the imaging system) during
acquisition.
What’s shading • Irregular illumination on the CCD chip can, e.g., lead to a dip in intensity
correction for? at image borders. This means that image edges are darker than the
central image area, thus specimen structures and image gray values
are no longer in clear correlation with one another. In this case, setting
of thresholds is impossible and successful automatic measurement op-
erations are also not feasible.
• The amplification of individual camera-array pixels can fluctuate slightly
in CCD cameras. Individual pixels - so-called "warm pixels" - are thus
somewhat brighter or darker than neighboring pixels which were illumi-
nated with the exact same intensity. This phenomenon can be particu-
larly disturbing when working with long exposure times.
What does shading The following image operations are executed during a real-time shading cor-
correction involve? rection:
5
1) The offset image is subtracted from the original image.
2) The resulting image will then be divided by the gain image - (the gain
image has already been offset corrected at this point - i.e., the appro-
priate offset image has already been subtracted from the gain image).
3) The resulting corrected image will then be multiplied by the mean gray
value of the offset-corrected gain image. Mean image brightness is
thus not affected by shading correction.
Shading correction Prerequisite for shading correction is that you have acquired a valid offset
prerequisites image and a valid gain image - using the Image > Acquire Reference
Images... command. When you begin image acquisition and your image
analysis program cannot find any valid reference images, you’ll be prompted
to acquire reference images.
Offset image A camera offset image is acquired without any illumination. An offset cor-
rection is necessary when using a CCD camera in order to compensate for
a non-homogeneous dark-current signal. A shading correction involves sub-
tracting the offset image from the original image.
KeenView • Defining offset and gain correction
To be precise, two offset images with varying exposure times are acquired
and saved. Common exposure times are 50 ms and 1000 ms. A third offset
image - when necessary - is then computed via inter- or extrapolation of the
first two offset images for the current exposure-time setting. If you alter
exposure time, and then acquire an image using real-time shading correc-
tion, the requisite offset image will be automatically recomputed without
notice.
A typical KeenView offset
image (this image has been
contrast enhanced).
Any structures resulting
from a non-homogeneous
dark-current signal are bare-
ly visible, but these can - es-
pecially where low-intensity
acquisitions are concerned -
lead to artifacts, which im-
pede quantitative image
analysis.
Gain image The camera gain image is acquired at homogeneous illumination - without
any specimen. The original image is divided by the gain image during shad-
ing correction.
A typical KeenView gain
image.
The non-homogeneous
illumination of the image
field is readily visible.
Also visible is the hexagonal
structure due to the fiber op-
tical coupling.
6
Warning! • If you have to work with more than one TEM acceleration voltage you
will have to acquire reference images for each of these. If your image
analysis program can directly read out the voltage set on your TEM via
remote control, it will automatically select the correct reference images
as needed. If you do not have remote control you will have to acquire
a new gain image whenever you alter the voltage level.
• You will also need to have reference images for each image format
which is defined in an input channel and is used to acquire images.
How to acquire reference images

A wizard guides you through the entire reference images acquisition. This
means that it allows you to successively acquire both offset and gain
images. Simply follow the onscreen instructions, select the relevant entries
and alter the microscope´s settings accordingly.
Note that you can only acquire a gain image if a valid offset image is avail-
able.
Input channels and The online shading correction and the reference images involved are con-
shading correction sidered part of the logical input channel. Therefore you have to acquire ref-
erence images for each input channel you want to activate the online shad-
ing correction for.
Both reference images will automatically be saved to the PC´s hard disk.
Acquiring offset 1) Switch on your TEM.
images 2) Make sure that no light can reach the camera’s CCD chip. To do this,
cover up the window of the TEM projection chamber and turn off the
electron beam or close the column valves.
3) In the Set Input dialog box, select the logical input you’re acquiring ref-
erence images with, typically the predefined KeenView Snapshot chan-
nel.
4) Select the Image > Acquire Reference Images... command to acquire
the offset image for correction of the camera´s dark current.
" The Acquire reference images dialog box will be opened.
The Acquire reference im-
ages dialog box.
The Info button, the Acquire
for current resolution only
option, and the Use the
current offest image check
box are not available when
you open this dialog box for
the first time.
5) If you acquire reference images for the first time, the Acquire for all
camera resolutions option will automatically be selected.
" If you wish to acquire reference images for the current resolution
only, select the Acquire for the current camera resolution only
option.
" If valid reference images are available, the Use the current offset
images check box is selected. In most cases, you will not need to
acquire offset images again.
6) Click the Next > button.
" You will be asked to configure the microscope in such a way that no
light illuminates the camera.
7) Then press Next >.
Acquiring offset images
" The offset image will be acquired.

" A progress bar and an estimation of the remaining time inform you
about the status of the procedure.
" As soon as the procedure is finished, you’ll be taken to the next
step.
8
8) Live acquisition is started

" You’ll be asked to remove the specimen and to check for
homogeneous illumination.
" Online Histogram and Camera Control are now open.
9) Use the to set the exposure time in such a way that the peak of the
histogram is situated at about 40% - 60%.
10) If your TEM-setup prevents the use of the whole image are of the
KeenView camera, click the Select area button to define a rectangular
area. The reference imagets will be constrained to this area.
Related topics
How to adjust the exposure time p. 11
11) Turn on the electron beam, remove the specimen from the ray path,
and click the Next > button.
" The gain image will be acquired and saved offset corrected.
Acquiring gain images
12) Click the Finish button.

" The online shading correction will automatically be activated.
How to switch shading correction off or on again
If you want to deactivate the shading correction later on, follow these steps.
1) Select the Image > Configure Input... command and activate the Input
tab.
Activating shading
correction via the Configure
Input dialog box.
2) Clear the Shading correction > Offset and Shading correction > Gain
check boxes.
3) Click on OK.
" Now all images you are acquiring via this input channel will not be
shading-corrected.
4) If the shading correction is activated in this way but the necessary
reference images have not yet been acquired, the Online-shading
correction wizard will be started as soon as an acquisition is attempted.
How to check the shading correction images

1) Select the Image > Acquire Reference Images... command.
" The Acquire reference images dialog box will be opened with step
1 active.
2) Click the Info button.

" The Information About Reference Images dialog box will be
opened, showing a list of all reference images for the active input
channel.
10
Related topics
How to acquire reference images p. 7
How to adjust the exposure time

1) Select the Image > Configure Input... command.
2) In the the Configure Input dialog box select the Display tab and select
the Online histogram check box.
3) Close the Configure Input dialog box
" Check the exposure time.
Adjusting exposure time.
5) Select the Image > Acquire command to switch over to the live mode.
" A live image will be shown in the active viewport.
" The online histogram window will be opened. The diagram shows
the current gray-value distribution of the live image.
6) Use the histogram to check whether the gray values are situated cen-
trally in the diagram. As necessary you can alter, e.g., electron beam
intensity or spot size of the electron microscope, and/or you adjust the
exposure time a little to shift gray values roughly into the middle of the
diagram.
" The mean value shown should be between 40 % and 60 %.
The mean value should be
between 40 % and 60 %.
11
7) Check that the mean value is between 40 % and 60 %. Then press OK.
Otherwise, use electron beam intensity and/or exposure time to adjust
the gray value distribution that way.
Then press OK.
How to check reference images

1) Set electron beam intensity at medium brightness.
" Generally, after having acquired reference images correctly, you
will not need to adjust current brightness. During the subsequent
image acquisition, the online histogram should display a mean val-
ue between 40 % and 60 %.
KeenView • Acquiring images using intX
2) Acquire an image - without specimen - using the Image > Acquire or

Snapshot command.
" The corrected acquisition should not have any visible structures
caused by the imaging system. The only thing you should be able
to see is homogeneous noise.
An offset and gain corrected
microscope image acquired
without specimen shows the
homogeneous random
noise visible when the
shading correction was
conducted successfully.
This noise is caused by the
electrons’ quantum noise.
The image has been
contrast enhanced.
3) Check that the exposure time in the Camera Control dialog box is the
standard value of the current input channel.
Camera Control
" If you need a different exposure time for standard, you have to ac-
quire new reference images which refer to this exposure time.
12
4) Make sure that the focus is correct before acquiring reference images.
Acquiring images using intX

intX The abbreviation intX stands for Intelligent eXposure. Use this acquisition
procedure to make perfect images with comfortable user functions.
Image resolution: A considerable advantage of using intX is that you can select different res-
acquisition and olutions for the live-image (acquisition) and for the snapshot. A lower image
snapshots resolution is suggested by default for the live-image since the frame rate is
then higher and thus the movements in the live-image are not choppy. If you
are taking a snapshot into the image buffer, it is recommended, however,
that you select the highest camera resolution possible in order to obtain opti-
mum image quality.
intX enables you to automatize the acquisition process to a large extent. The
exposure times for live images and snapshots are optimized independently
of one another. The optimization of the exposure time occurs continually and
automatically.
The Intelligent Exposure dialog box
XY calibration and intX intX uses the calibration data of the active input channel for the XY calibra-
tion of the images.
1) Select the Images > Intelligent Exposure... command.
" The Intelligent Exposure dialog box will open. While using this dia-
log box, you still retain access to all of your image analysis pro-
gram's other functions.
2) Click the Acquisition button to start the live-acquisition.
" The exposure times calculated by intX are displayed in the dialog
box's status bar. The term Live Exp. stands for the exposure time
of the live acquisition, while Snap Exp. stands for the calculated ex-
posure time of the snapshot.
3) Click the Online histogram On/Off button in the Standard button bar to
switch on the online histogram.
" The histogram shows you if the image is illuminated properly. The
distribution is cut-off to the right if the image is overexposed. If the
image is underexposed, the distribution shows a peak at the left.
4) Click the Snapshot button to acquire a single image.
" intX acquires a snapshot and writes it into the active image buffer.
A message informing you about the acquisition process appears if
the exposure time of a snapshot exceeds one second.

Intelligent Exposure offers a Click the Acquisition button to start the live-image. Click the Snapshot button
convenient alternative to the to end the live-image and to write the image into the image buffer.
acquisition commands Press the [Esc] key to terminate the live acquisition mode. Alternatively you
Image > Acquire and Image can click anew on the Acquire button located in the Intelligent Exposure dia-
> Snapshot.... .
log box.
13
Acquisition Click the Acquire button to start the live acquisition using intX. The live-
image is displayed in the image window. You can make the necessary set-
tings (focus, position the specimen) with the help of this live-image.
Characteristics of the The speed of the live-image has absolute priority when using intX. For this
live-image reason, it uses the camera's automatic mode-switch to increase the live-
image's frame rate. These changes, however, do not affect the snapshots
which were made with Intelligent Exposure.
Intelligent Exposure always makes use of the automatic display mode.
KeenView • Acquiring images using intX
Snapshot Click the Snapshot button to initiate the acquisition of a snapshot. intX
acquires an image and writes it to the active image buffer. A message box
informing you about the acquisition process appears if the exposure time of
a snapshot exceeds one second.
Exposure adjustment The Exposure adjustment slide control enables you to manually influence
the exposure time for snapshots calculated by Intelligent Exposure. Position
’0’ on the slide control does not influence the exposure time which was auto-
matically calculated by Intelligent Exposure. Move the Exposure adjustment
control to the right in order to increase the exposure time. The exposure
times for live acquisitions and snapshots are equally altered during correc-
tion, as long as the exposure time for snapshots is lower than 125 ms. The
exposure time for the live acquisition remains unchanged for exposure times
exceeding 125 ms.
Focus mode Click the Focus mode button if you want to focus the camera using the high-
est image resolution.
To enable focusing at the highest resolution, only a small image area is read
out of the camera in the intX mode. The size of the image area is 512x512.
The area position is predefined. The binning is automatically set to 1, so that
the entire resolution can be used in the selected area. The button is only
available in the live image.
Set ROI for Click the Set ROI for histogram calculation button to define a rectangular
histogram area in the image. The online histogram and the automatic display is only
calculation calculated inside this region of interest.
Move the ROI's frame shown in the image window to the desired position.
Set its size by moving the mouse and keeping the left mouse button
depressed. Rightclick to confirm position and size of the ROI.
Automatic gain display The intelligent Exposure always uses the automatic gain display to have the
image optimally displayed onscreen independent of the actual illumination
conditions. The system will assess the current intensity histogram in real
time in order to find the optimal onscreen display of the live image.
The automatic gain display affects monitor display only - it does not alter
actual image information.
When the automatic gain display is active, image intensity as far as monitor
display is concerned, will be spread between the minimum and maximum
14
values of the online histogram. Click the Reduce Contrast or Increase Con-
trast button to influence the maximum value. The higher the maximum value
the lower will be the contrast.
Options Click the Options... button to set the options for intX. The intelligent Expo-
sure Options dialog box will be opened.
The Intelligent Exposure

Options dialog box
Dynamic limit in % Set the dynamic range in which intelligent Exposure works with the Dynamic
limit in % slide control. Move the controller to the right to increase the
dynamic limit. This setting results in lighter images with a higher contrast in
darker areas of the image. Move the controller to the left to decrease the
dynamic limit. This setting results in darker images with a possible better
contrast in light areas of the image.
Max. exposure in s Set the highest exposure time to be used for snapshots using the Max. expo-
sure in s slide control. This value determines the maximum exposure time
which intelligent Exposure can use for snapshots. A warning occurs and the
images become darker should Intelligent Exposure calculate a higher expo-
sure time than determined by Max. Exposure in s.
Max. exposure focus Set the highest exposure time to be used in the focus mode using the Max.
mode in ms exposure focus mode ms slide control. This value defines the maximum
exposure time in the focus mode which Intelligent Exposure can use for live
image acquisitions. A warning will appear should this value be exceeded by
the current illumination conditions.
Binning intX may use binning in order to guarantee a high frame rate. Clear the
check boxes Acquire and/or Snapshot in the Binning group to disable bin-
ning for live acquisition and/or snapshot respectively. With the default set-
15
ting you will acquire high resolution snapshots while ensuring a high frame
rate for live acquisition.l
Keep live after Select the Keep live after snapshot check box, in order to continue the live
snapshot acquisition directly after a snapshot. A snapshot is created in the live acqui-
sition's image buffer and a new live acquisition is started in the next image
buffer defined in the sequence. Keep live after snapshot is only available if
you have selected an entry other than None in the Sequence list located in
the Special > Preferences > Image > Image acquisition dialog box.
Use the [Esc] button or click anew on the Acquisition button in the Intelligent
Exposure dialog box to quit the live acquisition mode.
Press the [Esc] key to terminate the live acquisition mode. Alternatively you
can click anew on the Acquire button located in the Intelligent Exposure dia-
log box.
Sharpness monitor for Select the Sharpness monitor for focus mode check box to use the sharp-
focus mode ness monitor as a focusing aid when working in the focus mode. Click the
Focus mode button in the Intelligent Exposure dialog box to activate the
focus mode.
KeenView • Calibrating input channel
Do not carry out ’Post Select the Do not carry out ’Post Processing’ when ’Acquire’ is switched off
Processing’ when check box to disable the postprocessing steps defined on the Macro tab of
’Acquire’ is switched the Configure Input dialog box.
off
Real time FFT Select the Real time FFT check box to have not only the live image shown
during a live acquisition, but the continually-updated fourier image shown as
well. This will simplify the adjustment and focusing of the TEM.
Online histogram Select the Online histogram check box to be able to keep an eye on the
intensity distribution during image acquisition. During image acquisition, a
window showing the current histogram will appear automatically. This histo-
gram will be updated continually.
Calibrating input channel

A calibration should be carried out for every input channel that is used to
make image snapshots. The input channel can be calibrated using the real
image or its fourier transform.
1) Insert a calibration standard sample into your microscope. This is nor-
mally a grid or grating with regular known intervals (e.g. a replica with
2160 lines/mm or 463 nm line spacing).
2) Select the Image > Set Input command. Select the input channel you
want to calibrate, usually a snapshot channel.
3) Set a reasonable microscope magnification.
4) Use the Image > Acquire command to optimize the acquisition para-
meters of the calibration sample.
5) Select the Image > Configure Input... command and activate the XY
Calibration tab.
Calibrating input channel.
16
6) Enter the current magnification level in the Magnification field.
Related topics
Sharpness Monitor p. 24
Using the RTFFT p. 18
Calibrating input channel
7) Enter the length you want to calibrate with in the Calibration length field.
8) Click the Unit... button.
" Select m (for meters) in the Basic unit list in the Set Unit dialog box.
" Select, e.g., n in the Scale list if you wish the calibration length to
be nm.
9) Select the Arbitrary option in the Calibration group.
10) Leave the X/Y ratio for the KeenView camera at 1.
11) Click the Calibrate button.
" The mouse cursor will appear within the active image.
" Position the mouse cursor at the starting point of the calibration
length and leftclick.
" Then position the mouse cursor at the final point of the calibration
length and confirm by leftclicking. The line must be the exact same
length as the calibration length you entered.
12) Click the Save... button to open a list of calibration data for various
magnifications.
13) Click the Add button to add the data of the calibration you’ve just com-
pleted to the magnification list and have it included in the diagram as
well.
14) If the list in the Magnification Table dialog box initially included invalid
calibration data, select these entries and click the Delete button.
Defining calibration curve.
17
15) Exit this dialog box by clicking on OK.

16) Repeat this for other magnifications to obtain a magnification calibra-
tion curve.
• To do so, you usually don’t have to leave the Configure Input dialog
box. Alter the microscope magnification. Click the Acquire button in the
Configure Input dialog box and use the live image onscreen to position
and focus the calibration specimen. When you’re satisfied, quit the live
mode by clicking the Snapshot button. Now you can repeat the calibra-
tion on that image.
17) When you have finished calibration, confirm it via OK.
KeenView • Using the RTFFT
Note When you have calibrated an existing channel, you don’t have to calibrate
new channels. You can transfer the calibration data of the existing channel
to any new channel.
To do so, select the Image > Set Input... command. Activate the existing
channel whose calibration data you want to transfer. Press the New channel
button. In the Select device dialog box, choose the KeenView entry. Select
the Use calibration of active channel check box and confirm via OK.
Using the RTFFT

The Real-time Fast Fourier Transformation (RTFFT) is a tremendous
help when interactively adjusting the electron beam in the TEM and when
focusing the image. During live acquisition, besides having the live image
shown, you can have the continually-updated fourier image (the absolute
value of its fourier transform):
The fourier image of a 2-D
catalase crystal.
The light spots represent the
periodic structures in the
microscope image. The
closer such a spot is located
to the center of the fourier
image the lower is the local
frequency of the corre-
sponding periodic structure -
i.e., the greater the periodic
distances within the micro-
scope image. (The light
‘cross’ is a result of margin
effects.)
Adjustment and focus- When working with thin, amorphous specimens, rings (so-called ‘Thon
ing using RTFFT rings’) result in the diffraction patterns of the TEM. The dark rings indicate
where the phase-contrast transfer function of the TEM is either minimal or
zero. The fourier image computed will also contain corresponding ring-
18
shaped structures. If these are elliptical in shape this indicates that an astig-
matism exists. If this astigmatism is corrected in the TEM, the ellipses within
the fourier image will become circular in shape.
Two fourier images of an
amorphous specimen.
The elliptical shape of the
structures in the left image
indicates an astigmatism.
The astigmatism has been
corrected in the right image:
the ellipses have become
circular.
Using the RTFFT
If the acquired image runs out of focus, the high local frequencies - i.e., the
structures located further away from the center - will disappear.
If you’ve switched on a Wobbler on your TEM that rapidly flips the electron
beam back and forth, fringes may appear within the fourier image. These
fringes indicate that the electron beam is not precisely focused on the spec-
imen. When you focus the beam the distance between the fringes will
increase and the fringes will then move out of the image.
Microscope image and
corresponding fourier image
of latex beads.
Two partial images overlap
one another in the micro-
scope image (left) because
the (not-precisely-focused)
electron beam is being
flipped back and forth by the
Wobbler.
This is why a fringe pattern
occurs in the fourier image
(right).
Activating RTFFT The RTFFT function with the RTFFT Display window and the RTFFT Control
dialog boxes is activated by selecting the Real time FFT check box in the
Image > Configure Input > Display tab.
Alternatively, click the RTFFT On/Off button located in the Standard button
bar to activate the display of the real time FFT.
Make use of the RTFFT Control dialog box to improve onscreen display of
the fourier image:
19
ROI On the ROI tab, you define the size (256x256 or 512x512) and the position
of the (centered) image segment (Region Of Interest) used to compute the
fourier image.
Select the Show ROI check box to set a red frame into the image which you
position with the mouse and whose size you can increase or decrease by
keeping the left mouse button depressed. The right mouse button enables
you to set the frame.
Visualization On the Visualization tab, you use the scroll bars to adjust contrast and
brightness.
Tools On the Tools tab, you select the 90°Rotation check box to tilt the image 90°
clockwise. This may be necessary - depending on the flange (the latter
depending on the microscope) - to obtain an image whose orientation
KeenView • Activating the remote control
matches that of the eyepiece image display.

Select the Overlay Circle check box to display a circle in the overlay of the
fourier image. The center of the circle is the origin of the fourier image. The
slide control enables you to manually modify its radius.
Click the Grab FFT button to create an identical copy of the fourier image as
a gray value image with 8-bit-depth of data. This image will be written into
the destination image buffer of your image analysis program.
Activating the remote control

Most microscopes have remote control capabilities that will allow your image
analysis program to read the current magnification of the microscope via a
serial cable connection. This ensures that all images acquired will carry the
correct calibration information assuming that the microscope or input chan-
nel is properly calibrated.
1) Select the Image > Configure Input... command and activate the Mag-
nification tab.
Activating remote.
20
2) The name of the microscope should be displayed in the Device list. Se-
lect this device.
3) Select the Remote > On check box.
4) Click the Read button to read the magnification.
" Magnification is automatically read via the remote control every
time a snapshot is made.
Using the Macro tab in the input channel

If you do not have remote control features on your microscope, then you are
required to set the magnification of the image whenever a snapshot is made.
Your image analysis system can help you not to forget setting the magnifi-
cation.
1) Select the Image > Configure Input... command and activate the Macro
tab.
The Camera Control dialog box
Setting the magnification

2) Type in the function SetMagnification$() in the Postprocessing steps
field. Put a semicolon at the end of the line.
" This makes sure that you will not forget to set the magnification of
the images that you acquire. The Set Magnification dialog box will
pop-up after each image snapshot.
3) Test the little macro. To do so, click the Execute button.
• If you receive an error message, check your spelling of the command.
• If the Set Magnification dialog box is opened, your macro will function
correctly.
Entering macro commands
to be executed.
4) Confirm the setting by clicking OK.

5) Click the Snapshot button located in the Standard button bar.
21
" The live image will be frozen.
" The Set Magnification dialog box will be opened. Enter the micro-
scope magnification and confirm via OK.

The Image > Camera Control... command and the appropriate dialog box
enables you to attain control over numerous setting possibilities required for
acquiring single images and live acquisitions. The commands in this dialog
box are closely interlocked with the settings in the Configure Input dialog
box.
The Camera Control command is only available if the Set Input dialog box
is closed.
Related topics
Configuring Input Channel p. 25
KeenView • The Camera Control dialog box
The Camera Control dialog

box for a KeenView camera
Exposure Time
Exposure Time You can set the camera's exposure time in the Exposure time group.
Click the - and + buttons to alter the exposure time in pseudo-logarithmic
steps. You can also directly change the exposure time in the corresponding
text field or manipulate it using the arrow buttons next to the text field. The
minimum and maximum exposure times depend on the camera being used.
Auto Click the Auto button to enable the automatic exposure time settings. The
Auto button is only available in live-mode.
Please note, that you may not see the effect of a higher exposure time on
the image displayed in the image window if you use the automatic display
function. In this case you should checl the exposure time using the online
histogram. If the online histogram is shifted to intensity values that are too
high, this may result in an overexposure of some image areas.
Automatic Gain Display and Fixed Scaling

The KeenView camera acquires images with an information depth of 12 bit.
This means that an image can comprise intensity values ranging from 0 -
4096. However, only 8 bits, i.e. 0-255 values can be used for the display.
Therefore, a display LUT has to be used to map the original intensity values
to an intensity range of 0-255.
Let’s take the following example. If the minimum intensity value of the origi-
nal image is 0 and the maximum intensity value is 2550, the following map-
ping function is one possibility to display the intensity values on the monitor.
All intensity values 0-9 are displayed using the intensity value 0. All intensity
22
values 9-19 are displayed using the intensity value 1 and so forth.
Keep in mind that only the monitor display is changed, not the raw image
intensity. When measuring image intensities you will always get the ’real’
ima ge intensity, not the image intensity that is displayed on the monitor.
However, there are different possible display LUTs for displaying the image
on the monitor.
Fixed scaling The "Fixed scaling" method uses a fixed intensity range that is used to dis-
play all images on the monitor. The limits for this fixed intensity range can
either be manually set or automatically calculated from the intensity histo-
gram. Use this method if you want to compare the brightness of different
images.
Automatic gain display The "Automatic gain display" method extracts the intensity range to be used
based on the actual signals on hand. Use this method to be sure to see
something on the monitor, even if the image is actually underexposed.
Use automatic Click the Use automatic gain display button to activate the automatic gain
gain display display. The live-image, independent from the illumination conditions, is
always shown in optimal contrast. The behavior of the "automatic gain dis-
play" depends on whether or not an desired overflow has been set and on
which area of the image the contrast enhancement is going to take place.
The desired overflow can be set using the Image > Configure Input com-
mand located on the Display tab. The following possibilities for selecting the
image area can be found in the Camera Control dialog box.
Please note, that the following methods for calculating the histogram affect
the automatic gain display and the histogram that is shown in the online his-
togram.
Histogram Click the Histogram calculation on full image button to carry out the contrast
calculation on optimization on the basis of the entire image. This option is recommended if
full image the structures to be observed are equally dispersed throughout the entire
image.
Histogram Click the Histogram calculation on crosshair button to only consider the
calculation on pixels along a strip which is only a few pixels wide in the horizontal and ver-
crosshair tical direction.
Histogram Click the Histogram calculation on ROI button to carry out the contrast opti-
calculation on mization on the basis of a rectangular area within the image. You can use
ROI this option if all of the relevant details are visible in a part of the image. The
display can thus be optimized for this area.
Set ROI for Click the Set ROI for histogram button to interactively set the rectangular
histogram area of the image for which the contrast will be optimized. This button is only
available if the Histogram calculation on ROI button is depressed. Move the
ROI's frame shown in the image window to the desired position. Set its size
by moving the mouse and keeping the left mouse button depressed. Right-
click to confirm position and size of the ROI.
Use fixed Click the Use fixed scaling button to use a fixed scaling range. The limits for
scaling the intensity range can either be automatically calculated from the image
histogram or manually set. For example, if the limits are 0 - 3825, all intensity
values 0-14 are displayed using the intensity value 0. All intensity values 15-
30 are displayed using the intensity value 1 and so forth. All intensity values
greater than 3825 are set to 255.
This display LUT will be used for all images and is not automatically adjusted
if the intensity range of the image changes. When using the fixed scaling,
the image is no longer displayed optimally if the illumination or sample are
altered.
23
Automatically Click the Automatically adjust fixed scaling button to automatically set the
adjust fixed scaling according to the current conditions. This button is only available dur-
scaling ing an active live acquisition.
Manually Click the Manually adjust fixed scaling button to manually set the limits for
adjust fixed the fixed scaling. The Fixed scaling dialog box will be opened.
scaling
Move the Left limit and Right limit slide controls or directly enter the desired
numerical value in the text field.
KeenView • The Camera Control dialog box
Sharpness Monitor
The Sharpness Monitor allows you to control the focus settings during a live
acquisition. You are also able to define a rectangular area of the image
which is to be analyzed for the sharpness monitor in the same way as for the
partial readout. The maximum contrast serves as a monitor for the sharp-
ness in this area. Therefore this area should be limited to an image segment
in which the contrast is a good monitor for the sharpness.
The live overlay must be enabled for the sharpness monitor. This can be
done via the Display tab located in the Configure Input dialog box.
Function of the The Sharpness Monitor consists of a dialog box in which a relative measure-
Sharpness Monitor ment of the sharpness is displayed by a changing bar which can be varied
between Blurred and Focused.
The Sharpness Monitor
If you start a live acquisition with an activated Sharpness Monitor or activate

it during a live acquisition, it will show middle sharpness (50%) in each case.
This is because there is no absolute measurement for the sharpness in an
image and because the sharpness monitor only gives relative values which
are related to the initial situation that was present when you started the live
acquisition.
Focusing and Sharpness If you alter the focus settings during a live acquisition with an activated
Monitor Sharpness Monitor, the length of the bar changes. In doing so, the green
marker bar shows the maximum sharpness reached since the live acquisi-
tion was started. The bar shifts accordingly to the right should the sharpness
increase due to your changes. If the focus settings are already optimal when
starting acquisition, the bar can only shift in direction Blurred.
Reset to 90% Click the Reset to 90% button to set the sharpness monitor's relative value
to 90%. Use this function if you start working with a very blurry image and if
the sharpness monitor's bar reaches the limit to the right while focusing. By
resetting to 90%, you provide the display with more room for additional
24
focusing. The Reset to 90% function is also available if the sharpness drops
very drastically due to changes made to the microscope settings during an
acquisition.
Make good acquisitions by setting the sharpness monitor as follows:
1) Set all other parameters (microscope settings, exposure time) optimal-
ly. The application of the sharpness monitor should be the last step be-
fore image acquisition.
2) Acquire a snapshot.
3) Use the Image > Camera Control... command.
4) Click the Set ROI for sharpness monitor button to interactively define a
rectangular area of the image for the sharpness monitor.
" To do this, select an area of the specimen for which a good sharp-
ness setting is especially important.
5) Start a live acquisition.
" The Set ROI for sharpness monitor button becomes inactive.
Related topics
The Display tab p. 27
Configuring Input Channel
6) Click the sharpness monitor on/off button to open the sharpness mon-
itor dialog box.
" The sharpness monitor begins with a value of 50%.
7) Vary the focus settings and observe the sharpness monitor while doing
so.
" The sharpness monitor bar shows the current sharpness, the green
marker shows the highest achieved sharpness.
" Pay attention to the exposure time when you set the focus. The
sharpness monitor reacts with a certain amount of delay when us-
ing larger exposure times (> 100 ms). Vary the focus settings in
small intervals and each time you do so, wait until the sharpness
monitor has reacted before making the next change.
8) Find the focus settings at which the sharpness monitor is at its maxi-
mum and use these settings for the acquisition.
Configuring Input Channel

Duplicating already existing input
Do not change the predefined input channels. If you want to configure the
input differently, first duplicate one of the already existing inputs. By doing
so, you retain all of the channel's settings.
1) Select the Image > Set Input... command.
" All current logical input channels are listed in the Set Input dialog
box.
2) Select one of the existing inputs, for example the KeenView Search in-
put.
3) Click the Duplicate channel button.
" The selected input channel will be copied together with its settings
and a new input channel will be created with these settings. The
number 1 will be added to the name of the input.
Configuring inputs
25
1) Select the Image > Set Input... command. Select an already existing in-
put.
2) Click the Configure Input button to define the properties of this input
channel.
" The Configure Input dialog box will be opened.
The Configure Input dialog box

The Configure Input dialog box contains several tabs for setting the proper-
ties of input channels. The tabs Input, Display and Format contained in this
dialog box are described in the following. These three tabs contain the set-
tings that apply specifically to the KeenView.
Viewing channel data When you acquire an image via a KeenView input channel its properties will
automatically be saved along with the image. This data can then be viewed
at a later point in time. To do so, load the image into an image buffer and
doubleclick on that image buffer in the image manager. The Image Informa-
tion dialog box will be opened. Channel properties are recorded in the Chan-
nel Data tab.
KeenView • Configuring Input Channel
The Input tab
Exposure Enter the number of micro-seconds the camera’s CCD chip is to be exposed
in the Exposure field. The chapter entitled "Camera Control" explains how
you can change the value in this field with the help of the Camera Control...
command interactively, while viewing a live-image.
Shading correction Use the Shading correction group to have rigid image structures corrected
during image acquisition - these structures may result from, e.g., an uneven
illumination of the image.
Select the Offset check box to have the offset image ("dark image") sub-
tracted during image acquisition.
Select the Gain check box to have the image be divided by the gain image
during image acquisition.
Note If you select the Gain check box, be sure to select the Offset check box as
26
well. Otherwise the shading correction will not work satisfactorily.
Info... Click the Info... button to view information on the device in use and the cur-
rent temperature of the CCD chip and the camera housing - in the KeenView
FW Information dialog box:
Related topics
Exposure Time p. 22
Defining offset and gain correction p. 5
The KeenView FW Informa-

tion dialog box
Temperature The CCD Chip field indicates the temperature measured directly on the CCD
chip. The operating temperature is 10 °C.
The Display tab
27
Over exposure The Over exposure group is where you determine when, or if, an overexpo-
sure warning is to appear. Your image analysis program continually checks
image intensity in the live mode and will warn you as soon as the live image
becomes too bright.
Select the Display warning check box to activate the overexposure warning.
An image is overexposed when a certain percentage of all pixels have

reached the maximum possible gray value. You enter this percentage in the
Overflow field.
Automatic gain display Use the functions of the Automatic gain display group to have the image
optimally displayed onscreen independent of the actual illumination condi-
tions. The system will assess the current intensity histogram in real time in
order to find the optimal onscreen display of the live image.
The automatic gain display affects monitor display only - it does not alter
actual image information.
Select the Activate check box to turn on the automatic gain display.
When the automatic gain display is active, image intensity as far as monitor
display is concerned, will be spread between the minimum and maximum
values of the online histogram. You determine these values in the Left over-
flow and Right overflow fields. Enter the percentage of pixels with the lowest
intensity values which are to be displayed black, and which percentage of
the highest intensity values are to be displayed white.
This is how you can clip intensity values that are to be ignored at both the
upper and lower ends of the histogram. Make use of the overflow values so
that individual pixels or minute image areas that are too bright or too dark do
not influence the automatic gain display.
Fixed scaling Instead of using the automatic gain display, you can use the fixed scaling.
The group whose Activate check box has been selected will be the one
available - the other will not be available.
The functions of the Fixed scaling group are for manually influencing
onscreen image display. You select definite minimum and maximum limits
for the intensity range that you wish to have optimally spread for onscreen
display. Any intensity value occurring outside of this defined intensity range
will appear either black or white. Enter the minimum intensity limit in the Left
limit field - the maximum intensity limit in the Right limit field.
As is the case with the automatic gain display, this manual definition of a
gray-value range affects monitor display only - actual image information is
not altered.
Online histogram Select the Online histogram check box to be able to keep an eye on the
intensity distribution during image acquisition.
Alternatively, click the Online histogram On/Off button located in the Stan-
dard button bar to switch on the online histogram.
28
The histogram is calculated either on the full image, along a crosshair or on

a user-defined ROI (Region of Interest). Use the button in the Camera Con-
trol dialog box to choose which histogram calculation you want to use.
Beneath the histogram, minimum, mean and maximum gray values of the
image will be shown. Percentages are in relation to the maximum obtainable
intensity value.
Related topics
The Camera Control dialog box p. 21
When working with the KeenView (with its 12-bit depth), the maximum
obtainable intensity value is 4096. This intensity value corresponds to 100%.
A mean of 85% would thus correspond to an intensity value of 3481.
Please note, that you can also switch between the automatic display and the
fixed scaling in the online histogram dialog box. To activate one display
mode just click the corresponding button.
Show histogram ROI Select the Show histogram ROI check box to display the ROI used for cal-
culating the histogram into the overlay of the live image. This check box is
available only if the live overlay has been activated. Please note that this set-
ting is only relevant if you have selected the Histogram Calculation on ROI
option in the Camera Control dialog box. By default, the histogram is calcu-
lated on the cross hair. To select another ROI, use the Set ROI for histogram
button in the Camera Control dialog box
Real time FFT Select the Real time FFT check box to have not only the live image shown
during a live acquisition, but the continually-updated fourier image shown as
well. This will simplify the adjustment and focusing of the TEM.
Live overlay Select the Live overlay check box if you want to use overlays in live-images.
Live overlays are absolutely necessary for a lot of acquisition functions, for
example, partial readout or Real time FFT.
The Live overlay check box is only available if your display board features a
so-called hardware overlay which is supported by Microsoft DirectX. Almost
any up-to-date AGP display board provides you with that feature. You can
look at the System Info dialog box to check whether your display board
offers this feature. To do so, select the Info... command from the ? menu and
click the System Info... button. The System Info dialog box will be opened.
Click the "DirectX" entry in the list on the left-hand side to have the desired
information appear in the list on the right-hand side.
Fixed zoom for binning If you modify the binning, the pixel resolution changes as well. This means
that the image is displayed in a different size in the image window. Select
the Fixed zoom for binning check box to always attain the optimum live
image quality on the monitor no matter what the current binning settings are.
By default, the zoom factor for the live image in the image window is set to
100% for the 4x4 binning. If you select another binning, the zoom factor is
adjusted so that the image size remains unchanged.
29
Image scaling Select one of the four options for displaying an image in a viewport in the
Image scaling list.
Select the Underscan entry to have the whole image displayed. The system
will select the largest zoom level - 25%, 50% or 100% - with which the whole
image can still be displayed within the viewport. In certain cases not all of
the viewport’s available area will be used.
Select the Overscan entry to ensure that all the viewport’s available area will
be used. The system will select the smallest zoom level - 25%, 50% or 100%
- with which the image fills the whole viewport. In certain cases parts of the
image will not be visible.
Select the Adjust to viewport entry to have the image size adjusted to opti-
mally fit the viewport. The image will be adjusted to fit the current size of the
viewport.
Related topics
The Camera Control dialog box p. 21
Using the RTFFT p. 18
Select the Full size (100%) entry to have the image displayed without any
zooming at all. If the viewport is smaller than the image, as much of the
image will be shown as fits - starting from the upper-left corner of the image.
Histogram calculation Select the pixels to be taken into consideration for calculation of the gray-
value histogram in the Histogram calculation list.
These settings effect all real time functions which use the gray-value histo-
gram: over exposure warning, Automatic gain display, and the Online-Histo-
gram. There are three options:
Full image If you select the Full image entry, the gray values of all pixels will be applied
to histogram calculation. This is the most commonly used setting when us-
ing light microscopy.
Crosshairs If you select the Crosshair entry, only pixels of a particular image area are
used in the calculation. This image area is shaped like cross hairs that are
centrally positioned on the image. The vertical and horizontal segments of
these cross hairs are respectively just a few pixels wide and cover just about
the entire height and width of this image.
ROI Select the ROI entry to carry out the optimization of contrast and exposure
time based on a rectangular frame.
Application We recommend selecting Crosshair if you wish to speed up histogram cal-
culation. You can speed up the frame rate of the live-image depending on
what real time functions you have activated. You should however make sure
that the pixel gray values around the middle image line and around the mid-
dle image column are representative for the gray values of the entire image.
If this is the case, you will not notice any difference compared to the Full im-
age option for the over exposure warning, Automatic gain display, and the
online histogram. The ROI method is recommended if only a section of the
image is relevant for the calculation of the histogram and the rest of the im-
age is not to be used for this calculation.
Display palette You can select a false color lookup table from the Display palette list to color
the acquired image. The Display palette list is only available in black & white
mode.
- none - Select the entry - none -, to not use any palette for the image display.
Adjust, Dither, Gamma, The entries in this list are the names of various lookup tables which can be
30
Mixmap Rainbow, So- used for the image display on the monitor.
laris, Thermal
The Format tab
Image format The Image format list contains a series of predefined standard image for-
mats. These image formats are achieved using a combination of binning and
clipping.
The very first time you call up this tab the maximum obtainable resolution of
KeenView is preset. Images will not be clipped when you use to this setting.
In addition, this list contains all user-defined image formats. You can define
your own image formats in the Clipping border (pixel) group. User-defined
image formats are denoted by the appearance of the word "free" along with
the entry - e.g., "free, 450 x 334 Pixel, Binning 8 x 8". You may select either
a predefined or a user-defined image format from the list. The format you
select will be the one set the next time you call up this tab.
A graphic display of clipped borders can be seen in the Active area field. The
31
values are shown in the fields of the Clipping border (pixel) group.
Binning is when the camera integrates the contents of neighboring pixels.
This will result in a more speedy read-out and greater sensitivity - image res-
olution will however decrease.
Use binning when you, e.g., are working with an electron microscope at low
intensities because binning will reduce noise. Binning can also be useful if a
rapid frame rate is of greater priority than a high image resolution.
Horizontal Enter the number of neighboring pixels to be integrated into the Horizontal
Vertical and Vertical fields.
You cannot alter the values in the Horizontal and Vertical fields indepen-
dently of one another. If you alter one value, the other will automatically be
adjusted accordingly.
Clipping border (pixel) Define the number of lines and columns, and the edges where they are to
be clipped in the Clipping border (pixel) group. The corresponding format will
be indicated in the Image format field and displayed (graphically) in the
Active area field.
Enter the number of lines and columns to be clipped in the Top, Bottom, Left
and Right fields.
Click the Interactive button to define borders directly within the image. This
button is only available if the active image buffer contains an image at the
full resolution (1 x 1 binning) of the logical input channel. A red frame will
appear within the image. This frame can be enlarged or reduced in size by
keeping the left mouse button depressed and adjusting the frame size by
moving the mouse accordingly. Position the frame by moving the mouse
(the left button no longer depressed). Rightclick to confirm the frame. The
image area outside of the frame becomes the image borders and thus will
be clipped.
Click the No Clipping button to reset the number of lines and columns back
to zero ("0") - meaning that no borders will be clipped. The Image format field
will once again show the maximum image format corresponding to the res-
olution of the logical input channel.
For estimation purposes, the Active area field will provide you with a sche-
matic preview of how much area is to be clipped at the image borders. The
blue frame represents the maximum image format available for this logical
input channel. The cross-hatched area represents the image area. The
white areas represent the borders to be clipped.
32
Quick-start Installation
Quick-start Installation
This section serves as a guide for those who have installed a KeenView sys-
tem before. It is a quick reminder for a sequence of steps that should be fol-
lowed for a successful installation.
If you are installing for the first time or you are unsure of the procedure then
please skip this section and read all other sections carefully before begin-
ning the actual installation.
The basic installation steps are:
1) Install a FireWire board if not yet existing.
2) Air the TEM viewing chamber.
3) Remove TEM flange cover.
4) Install TEM specific adapter flange.
5) Remove protective cover on scintillator.
6) Install KeenView to adapter flange.
7) Evacuate the TEM viewing chamber.
8) Connect the Remote Control cable from TEM to the serial port of the
PC (normally COM1), if this option is available.
9) Install Remote Control software, if this option is available. Note that for
some microscopes, the remote control is installed during installation of
your image analysis program.
10) Install the image analysis program.
11) Install camera driver (Windows XP only).
12) Connect the cable from the KeenView to the FireWire board on PC.
13) Set up remote control for all input channels, if this option is available.
14) Set up offset and gain correction image for the two image formats.
15) Calibrate input channels.
33
Related topics
Software Installation under Windows XP p. 35
Software Control of the KeenView camera p. 3
KeenView • Camera Installation Procedure
Camera Installation Procedure

FireWire board IEEE-1394
When is this necessary? This procedure is necessary only if the PC does not have a FireWire on-
board hardware configuration and is not yet equipped with a FireWire board.
The FireWire board
How to install the FireWire board

1) Turn off the PC and disconnect the power supply.
2) Remove the cover from the PC.
3) One PCI slot is required for the FireWire board. Insert the board into the
slot.
FireWire board installed
34
4) Replace the cover onto the PC.

5) You are now ready to first mount the camera onto your microscope,
then to install the image analysis program onto your PC, and finally to
connect the camera with your PC.
Software Installation under Windows XP
Software Installation under Windows XP

Remote control software
Remote Control driver Remote control is available for some later models of microscopes. It allows
software your image analysis program to read some microscope parameters, such as
magnification, via the serial port. It is also used to send control commands
to the microscope, such as moving the sample stage.
Certain models of microscope require a separate installation of the Remote
Control driver software. This should be done before installation of your
image analysis program. This software is normally supplied in a separate
disk media and installation instruction.
Some models of microscope will need the remote drivers to be installed dur-
ing installation of your image analysis program.
Image analysis program, camera driver, and remote control

System requirements The KeenView camera requires the software analySIS or iTEM and
Microsoft Windows XP (or higher). Your image analysis program also sup-
ports multithreading on multiprocessors, thus making full use of the power
of the latest multi-CPU PC’s.
Camera driver instal- Installation of the KeenView camera driver starts via your image analysis
lation program setup. You are required to select FireWire and specify the addi-
tional camera driver during setup. Then you have to get Windows to use the
KeenView camera driver.
analySIS or iTEM setup Installing the analySIS or iTEM software is a simple process following on-
screen instructions.
1) Place the installation CD of your image analysis software into the CD-
ROM drive.
" The setup program will start automatically - unless you have deac-
tivated the autorun function. If so, start the setup.exe file manually
via Windows Explorer.
2) Follow the installation wizard.
35
Related topics
Camera driver installation p. 39
KeenView • Software Installation under Windows XP
Carefully read the License

Agreement and accept it by
clicking Yes.
Select the appropriate

model of microscope.
36
Image analysis program, camera driver, and remote control
Then select the KeenView

entry.
Note Your image analysis program can support several different types of FireWire
cameras simultaneously.
3) Clickt Next > and finish the installation.
37
Camera cable
How to connect camera and PC
1) Plug one end of the FireWire cable into the camera connector.
" The FireWire cable plug is not symmetrical. Make sure that the
cable plug is facing the same way as the camera socket when
you plug the cable into the camera connector. The camera chip
may be ruined otherwise.
Make sure the cable plug is
facing the right way before
you plug it into the camera
socket.
2) Plug the other end of the FireWire cable into one of the small connector
sockets on the FireWire board.
" There are two or more connector sockets depending on the
FireWire board. You can use any of them.
3) Continue with the camera driver installation before starting your image
analysis program.
38
Camera driver installation

After having installed your image analysis program and connected the cam-
era to the PC the camera is not yet ready for operation. You have to go
through the following steps to get Windows XP to use the correct camera
driver.
1) Open the Windows Device Manager via Start > My Computer > (right
mouse) > Properties > Hardware > Device Manager... .
" The Device Manager dialog box shows the Generic 1394 Desktop
Camera entry in the Imaging devices section. The entry is marked
with a warning sign.
2) Select the Generic 1394 Desktop Camera entry and click the Update
39
button in the device manager button bar.
3) Select the No, not this time option and click the Next > button.
4) Select the Install from a list or specific location (Advanced) option and
click the Next > button.
40
5) A new Hardware Update Wizard dialog box will be opened.

6) Select the option Don’t search. I will chose the driver to install. and click
the Next > button.
" A list with differrent compatible drivers will be shown.
The driver for your Keen-
View camera has been
found.
41
7) Select the Olympus Soft Imaging Solutions FireWire Camera entry

from the list.
" The following dialog box appears.
9) Click the Continue Anyway button.

" The Hardware Update Wizard dialog box which had been in the
background, will become visible.
" After you have finished the KeenView camera driver installation,
the following dialog box will be opened.
42
10) Click the Finish button.

" The Hardware Update Wizard dialog box will be closed.
" The warning sign for the entry Generic 1394 Desktop Camera in the
Imaging devices section of the Device Manager dialog box is now
gone.
" The camera is now ready for use.
Troubleshooting
Troubleshooting
What to do when the 1) Open the Windows Device Manager via Start > My Computer > (right
driver siscamfw has not mouse) > Properties > Hardware > Device Manager... .
been found.
" The Device Manager dialog box shows the Generic 1394 Desktop
Camera entry in the Imaging devices section.
2) Select the Generic 1394 Desktop Camera entry and click the Update
button in the device manager button bar.
43
3) Select the Install from a list or specific location (Advanced) option and
4) Select the Don’t search. I will choose the driver to install. option and
44
" The Model list contains the siscamfw entry.

" The next Hardware Update Wizard dialog box will be opened in the
background displaying the message to wait while the Wizard in-
stalls the software.
" Additionally, the Hardware Installation dialog box will pop up in the
foreground.
Troubleshooting
6) Click the Continue Anyway button.

" The Hardware Update Wizard dialog box which had been in the
background, will become visible.
" After having finished the KeenView camera driver installation the
following dialog box will be opened.
45
KeenView • Appendix
Appendix
Technical specifications
Image resolution Standard resolution: 1376 x 1032
2x binning: 688 x 516 pixel
Pixel size 6.45 x 6.45 µm
Pixel clock rate 20 MHz @ 12 bit
Dynamic range 4096 gray levels @ 12 bit
Exposure time 100 µs - 160 s
Frame rate (depending on reso- Standard resolution: > 10 fps
lution and PC speed) 2x binning: > 20 fps
Display Full image in real time
Cooling element 10°C @ 25° ambient temperature, Peltier
cooled
Temperature monitor CCD chip & Housing
Camera position on axis
Camera coupling tapered fibre optics
Anti-Blooming 300 x
Interface FireWire (IEEE1394)
Packing List
1) KeenView camera unit assembly.
2) TEM specific adapter flange.
3) analySIS PC with software and FireWire board installed. (If complete
system is delivered.)
4) FireWire cable (PC to camera).
5) Image analysis software package.
6) This User’s guide
46
Index
A L
Acquire Images 3 Live Acquisition
Automatic Gain Display intX 13
Camera Control 22 Live overlay 29
C O
Camera Control 4, 21 Offset image 7
Exposure time 22 Online shading correction 5
Gain display 22
Sharpness Monitor 24
Camera resolution Q
in intX 15
Check reference images 11 Quick-start installation 33
Configure Input
Input 26
R
Remote control 20
D Resolution
Display tab intX 15
Palette 30 RTFFT Control 19
E S
Exposure time Set Magnification 20
Camera Control 22 Sharpness Monitor 24
Snapshot 5
intX 14
Software Installation
F
47
Windows XP 35
FireWire board IEEE-1394
Installation 34
W
Windows XP
I Software Installation 35
Image acquisition
RTFFT Control 19
Input Channel 3
Calibrate 16
Input channel
Configuring the input 25
Duplicating already existing input 25
intelligent Exposure 13
intX 12, 13
Exposure time correction 14
Resolution 15

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© Olympus Soft Imaging Solutions GmbH

ACQUIRING IMAGES ...............................................................3

Camera Installation Procedure 34

Software Installation under Windows XP 35

Technical specifications .....................................................46

Software Control of the KeenView camera

Predefined input channels

Scanning the specimen

2) Select the Image > Camera Control... command.

4) Locate the sample position you are interested in.

" The Histogram dialog box will be closed.

Defining offset and gain correction

How to acquire reference images

Acquiring offset images

" The offset image will be acquired.

8) Live acquisition is started

12) Click the Finish button.

How to check the shading correction images

2) Click the Info button.

How to adjust the exposure time

How to check reference images

2) Acquire an image - without specimen - using the Image > Acquire or

Acquiring images using intX

The Intelligent Exposure dialog box

The Intelligent Exposure

Calibrating input channel

6) Enter the current magnification level in the Magnification field.

15) Exit this dialog box by clicking on OK.

Using the RTFFT

matches that of the eyepiece image display.

Activating the remote control

Using the Macro tab in the input channel

Setting the magnification

4) Confirm the setting by clicking OK.

The Camera Control dialog box

The Camera Control dialog

Automatic Gain Display and Fixed Scaling

If you start a live acquisition with an activated Sharpness Monitor or activate

Configuring Input Channel

The Configure Input dialog box

The Input tab

well. Otherwise the shading correction will not work satisfactorily.

The KeenView FW Informa-

The Display tab

An image is overexposed when a certain percentage of all pixels have

The histogram is calculated either on the full image, along a crosshair or on

The Format tab

Camera Installation Procedure

How to install the FireWire board

4) Replace the cover onto the PC.

Software Installation under Windows XP

Image analysis program, camera driver, and remote control

Carefully read the License

Select the appropriate

Then select the KeenView

Camera driver installation

5) A new Hardware Update Wizard dialog box will be opened.

7) Select the Olympus Soft Imaging Solutions FireWire Camera entry

9) Click the Continue Anyway button.

10) Click the Finish button.

" The Model list contains the siscamfw entry.

6) Click the Continue Anyway button.