Name Karen Paula Manalo Score

Group 2 Date Nov. 23, 2018

WORKSHEET 5

ENZYMATIC ACTIVITIES IN BACTERIA

1. Fill in Table 5.1 with the following results: A for acid production only, G for
gas production only, AG for both acid and gas production, and N for no
change.

Table 5.1. Fermentation reactions of the test bacteria

Bacteria Sugars
Glucose( Fructose Lactose Sucrose Mannitol Galactose
1) (5) (3) (4)
S. aureus A A A A A A
M. luteus N N N N N N
P. aeruginosa N N N N A N
Unknown A - N A A -

2. E. coli ferments glucose but not sucrose although sucrose is a disaccharide

of glucose and fructose. Explain why this bacterium uses glucose and not
sucrose. (2 pts)
E. coli does not have a sucrose operon responsible for sucrose
fermentation. However, many studies have already developed E. coli
strains that can ferment sucrose (Lee et al., 2010).

3. Fill in Table 5.2. Place a (+) for positive result and a (-) for negative result.

Table 5.2. Methyl Red and Voges Proskauer Test of the Bacteria
Test Bacteria Methyl Red Voges-Proskauer

E. aerogenes - +
E. coli + -
Unknown + -

4. Would it be possible to have an MR+ and VP+? Provide an explanation. (2

pts)
_

_
5. Fill in Table 5.3. Place a (+) for positive result and a (-) for negative result.

Table 5.3. Catalase and oxidase Test of the Bacteria

Test Bacteria Catalase Oxidase

S. epidermidis + -
S. aureus + -
P. aeruginosa + +
E. coli + -
Unknown

6. Fill in Table 5.4. Place a (+) for positive result and a (-) for negative result.

Table 5.4. Utilization of urea, gelatin and citrate

Test Bacteria Urea Gelatin Citrate

S. epidermidis + + -
S. aureus + + +
P. aeruginosa +/- + +
E. coli - - -
Unknown

7. Fill in Table 5.5. Place a (+) for positive result and a (-) for negative result.

Table 5.5. Utilization of amino acids

Test Bacteria Tryptophan Cysteine Lysine Phenylalanine

S. epidermidis + + + -
S. aureus - + - -
P. aeruginosa + + - -
E. coli + + + -
Unknown
 8. Identify the unknown bacteria
_

9. Conclusion
_

10. References

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Identification of Pseudomonas aeruginosa. Online Microbiology Notes. 2018
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Bortolotti P, Hennart B, Thieffry C, Jausions G, Faure E, Grandjean T, Thepaut

M, Dessein R, Allorge D, Guery BP, et al. Tryptophan catabolism in
Pseudomonas aeruginosa and potential for inter-kingdom relationship. BMC
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Carbohydrate Fermentation Test: Uses, Principle, Procedure and Results -.

Home -. 2016 Dec 10 [accessed 2018 Nov 23].
https://microbeonline.com/carbohydrate-fermentation-test-uses-principle-
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European Bioinformatics InstituteProtein Information ResourceSIB Swiss

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https://www.uniprot.org/uniprot/A0A0H2VKT7

European Bioinformatics InstituteProtein Information ResourceSIB Swiss

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Nov 7 [accessed 2018 Nov 23]. https://www.uniprot.org/uniprot/Q02RW8

European Bioinformatics InstituteProtein Information ResourceSIB Swiss

Institute of Bioinformatics. Tryptophanase. Drs - Drosomycin precursor -
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Biochemical Test and Identification of Staphylococcus aureus. Online
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https://microbiologyinfo.com/biochemical-test-and-identification-of-
staphylococcus-aureus/

Halsey CR, Lei S, Wax JK, Lehman MK, Nuxoll AS, Steinke L, Sadykov M,
Powers R, Fey PD. Amino Acid Catabolism in Staphylococcus aureus and the
Function of Carbon Catabolite Repression. mBio. 2017;8(1).
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Long S. Principles and practice of pediatric infectious diseases. 4th ed. 2012.

Microbiology Tests. Antranikorg. [accessed 2018 Nov 23].

https://antranik.org/microbiology-tests

Microbiology 20 Biochemical Unknown. 2009 [accessed 2018 Nov 23].

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Nilsdotter-Augustinsson A, Wilsson A, Larsson J, Stendahl O, Ohman L,

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epidermidis, modulates the oxidative response and induces apoptosis in
human neutrophils. Apmis. 2004;112(2):109–118. doi:10.1111/j.1600-
0463.2004.apm1120205.x

Otto M. Staphylococcus epidermidis — the accidental pathogen. Nature

Reviews Microbiology. 2009;7(8):555–567. doi:10.1038/nrmicro2182

Wu Y, Yu K, Xu B, Chen L, Chen X, Mao J, Danchin A, Shen X, Qu D, Jiang H.

Potent and selective inhibitors of Staphylococcus epidermidis tryptophanyl-
tRNA synthetase. Journal of Antimicrobial Chemotherapy. 2007;60(3):502–
509. doi:10.1093/jac/dkm229

Note: Some tests were not performed in the lab. Please research on the
positive results for these