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Page IV-1
FPE 31806 – Sustainability in food chains
physically modifies the starch into a form that can be digested well, and may denature of degrade
the anti-nutritional factors. Many vegetables hardly have any nutritional value without having
been cooked, as this opens up their strong, impermeable cell walls, and makes their contents
accessible for our digestive fluids.
A third motivation for processing in our modern society is that not everyone can or wants to spend
the full time necessary to produce a good meal from the raw ingredients. The development of
‘convenience foods’, i.e., foods that have been processed such that the consumer does not need
to spend much time in the kitchen, has been important in the emancipation movement, and
therefore in the development of the industrial world1.
In general, processing comprises some preliminary treatment done at the farm (before
transportation), and processing after transportation to a centralised factory. Some products may
be ready at this stage, and are transported, may be chilled, stored and sold by groceries: fruit,
vegetables, potatoes, rice, and milk. Other products merely act as ingredients in other products:
sugar, wheat flour, tomato paste, non-roasted coffee beans, cocoa nibs and butter, soybeans, malt.
They are therefore transported to other factories, and are converted into so-called processed
foods. One can think here of ice cream (produced from milk powder, sugar and a few other
components), soft drinks (produced from glucose or fructose syrup and concentrates), beer
(produced from malt, which in its turn is produced from barley), bread (wheat flour and yeast),
pasta sauce (from mashed tomatoes that have been concentrated and spices), but also cheese,
salami, Serrano ham, etcetera.
The food processing sector is essential in providing us all with the foods that we want, any time of
the year. It is the most important industry in the EU, with a turnover of €900 billion by about 310
000 companies, and provides jobs for more than 4 million people2.
1
It is however not true that convenience foods are only of our times. In the past, people often were
travelling, and even in prehistoric times, people needed to have quick food when hunting for several
days.
2
Interestingly, if we would not have a food processing industry, but would prepare all our foods at
home from raw materials, our energy and water expenditure would magnify to humungous amounts.
Processing foods on large scales enables us to re-use heat and water many times, and to generate it in
efficient ways. At home we have to use natural gas or even electricity for heating and we can only use
it once. Similarly, leftovers can be used for other products in a factory, while they most of it may be
thrown away at home.
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Chapter IV – Sustainable Food Processing
that form the basis of our industrial society. Even though their report ‘Limits of Growth’ created a
lot of turbulence, the net effect was small, as prices of oil, coal and ores remained relatively low.
From about 1990, people started to realise that health cannot be taken for granted. It was found
that many diseases (cardiovascular diseases, diabetes, some forms of cancer) have a close relation
to the type of diet that one follows. A trend towards healthier foods emerged, all the way towards
the modern so-called functional foods, which usually have an added component that is supposed
to have a specific (beneficial) effect on our health. In 2006 the prices of staple foods suddenly
started to rise, while at the same time (fossil) fuel prices went up. There was much turmoil around
the realisation that human activities produce enormous quantities of greenhouse gases, and the
Earth therefore warms up slowly. In an attempt to reduce the dependency on fossil fuels (which
are generally found in instable regions in the world) the US government then decided to establish
an industry that converts part of their corn harvest into ethanol by fermentation. Similar initiatives
were taken elsewhere, albeit not at the same scale. In the EU, gasoline is supplemented with a
small amount of bioethanol, and diesel fuel has to contain a few per cent biodiesel, for example.
Only from about 2008, the world has realised that something needs to change. First of all, the
industry was confronted with sudden and drastic increases in prices of food commodities and
energy. These price increases were caused partly by the use of agricultural products for energy
and fuel, partly due to the fact that East Asia developed its economy at an enormous rate and
needed the resources to sustain that, and partly due to large-scale speculation. There was a
general worry that the shortage on raw material and energy would be permanent. Even though
the prices have now stabilised, they are till on a higher level. The structural causes are still there:
many regions in the world are still developing rapidly, and need resources to run their factories,
provide their people with houses and cars and enable the people to run their cars, central heaters
and air conditioners.
Now we see a new, even larger threat: global warming. All human activities combined emit a lot
of greenhouse gases (CO2, CH4, NOx, O3 and others), and this causes the planet to heat up. This has
triggered an auto-accelerated process where the heating up melt part of the ice caps, which reduce
the albedo (reflectance) of the Earth, and release of even more greenhouse gases from previously
frozen permafrost tundra, leading to even stronger warming. This already causes droughts and
floods, storms and landslides, which have already taken a toll on global harvests. Therefore, the
expectation is that in the coming decades the agricultural productivity will be under stress; even
as the world human population will continue to rise towards 9 to 10 billion.
This realisation has led to a number of international agreements, such as the Paris Agreement in
2015 and ratified (with the exception of a few important countries) in 2018, which compels the
members to reduce their greenhouse emissions such as to remain below a 2℃ increase in global
average temperature. This has been translated in a strong reduction of the use of fossil fuels, and
much better use of feedstock. The government of The Netherlands has translated the guidelines
into a target of reducing the use of greenhouse gas emissions by 49% in 2030, and 90% in 2050.
All industry sectors are expected to contribute to this. The agro-food sector is one of these sectors.
Also the general public is now well aware of the need for reduction in fossil fuels and greenhouse
gas emissions. This means that for the first time, sustainability is a marketing issue, also for food
products. It is clear that this has attracted the attention of major players in the food industry. As
an example, Unilever launched its Sustainable Living plan in 2010, which specifies amongst others,
that they will half their environmental impact while growing their business. This includes the
emission of greenhouse gases, the use of water, more sustainable sources (i.e., not only focusing
on their own operations) and reducing the use of packaging and the production of waste.
To become more sustainable, the food industry has to work along several paths. Of course they
will continue to improve the efficiency of their current processes, and while this will continue to
deliver small improvements, this will not bring the emission anywhere near the large reductions
that have been agreed. Therefore they have to take radical steps. These are aimed at processes
that are inherently more efficient, but also at the use of raw materials that are more efficient to
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FPE 31806 – Sustainability in food chains
produce. And creating more product out of the same raw material obviously will reduce the
emissions per kg product as well.
In the Sustainable Food Initiative, a collective of the Dutch (-based international) food industry,
the objectives have been formulated as ‘develop the 4th generation food processing technology
enabling a decrease in footprint (Water, GHG) of 50 % in 2025 whilst preserving the inherent
goodness of agricultural raw materials; enable us to reduce waste by 50 % in 2025, enable better
than 95% use of agricultural raw materials and ensure full circularity of packaging materials by
2025.’ In addition, it commits that it should ‘be able to handle 90 % of available raw materials and
raw materials qualities in 2025.’
It is clear that the food industry can only make this transition, by combining this with attention to
the other two big societal challenges it faces: the global epidemic of overweight, and the loss of
trust that consumers have in processed foods.
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Chapter IV – Sustainable Food Processing
An alternative process, mostly used for non-food purposes (e.g., production of bio-ethanol) is the
dry corn milling process. Here, the corn is cleaned and milled into dry flour. It is then mixed with
water. The slurry is heated for gelatinisation of the starch and enzymes are added to hydrolyse it
into single sugars (glucose), after which the slurry is fermented by yeast into ethanol. The gluten
is not separated but present during the fermentation, and is partly consumed by the yeast. The
concentrated and dried remains after the fermentation is called the Distillers Dried Grain with
Solubles (DDGS). It is mainly used as cattle feed.
While the wet milling process produces good, food quality products, it is quite wasteful in terms
of water and energy usage. The dry milling processes is simple and effective, but very coarse, and
3 C.Galitsky, E. Worrell, M.Ruth, Energy Efficiency Improvement and Cost Saving Opportunities, for the Corn Wet
Milling Industry, Lawrence Berkeley National Laboratory, report LBNL-52307
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FPE 31806 – Sustainability in food chains
wastes a very good crop into low-value, low-quality products: bio-ethanol4 and DDGS. Therefore,
researchers from Cargill (one of the largest corn processors in the world) developed the CDCM
(Cryogenic Dry Corn Milling) process5. Here, the corn kernels are cleaned and then soaked in water
for a short time, during which they take up about 20 – 25 wt% water. During soaking, water seeps
in between the bran (pericarp), the germ and the rest of the kernel. The kernels are then frozen.
As you know, water expands when freezing. This effect is strong enough to break rocks when the
water is inside its cracks. It is the same in the corn kernel: the water that freezes in between the
kernel and the bran expands and causes it to separate. A simple mechanical rubbing is then
sufficient to remove the bran and the germ.
The rest of the kernel – mostly starch and gluten – is milled while frozen. At low enough
temperatures, all materials become brittle at some stage. The temperature at which this occurs is
called the glass transition temperature. This transition is different for each material, and for
biomaterials, is dependent on the amount of water present in the material. A brittle material can
be easily milled into a fine powder; a ductile material (above the glass transition temperature) is
just deformed and yields to it. Therefore, if we mill at a moisture content and temperature at which
one of the constituents is brittle, and the other one is not, we may get good separation of the two:
one constituent will fracture and make small particles; the other will stay intact and form larger
particles. These particles can then be separated by for example wind sifting.
Since the only water introduced is with the initial soaking (in which the moisture cont goes from
16 to 25 wt% (or only an addition of 0.397 kg water), the total energy requirement (which generally
is mostly needed for dehydration) is only a fraction that that of the conventional wet milling
process. Interestingly, the quality of the gluten and starch fractions are better compared to the
ones obtained from the conventional process as well. The process has not yet been applied on
commercial scales.
4 Bio-ethanol is basically a very low value product. Corn growth on the land costs a lot of soil tillage, fertilizer and
other stuff, and the processing needs a lot of energy, especially for the distillation of the ethanol from the
fermentation broth: if the ethanol concentration becomes larger than about 12%, the yeast is inactivated.
Therefore, there has to be a lot of water present (around 80% at the end), which makes it costly to isolate the
ethanol The DDGS is a heavily degraded fraction, which can only be used as additive in cattle feed, for its nitrogen
content.
5 US patent US20020086097; similar work has also been done with cereals and legumes.
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Chapter IV – Sustainable Food Processing
Figure 2: Material, water and energy streams in the current wheat processing system. The thickness of the arrows
indicates the size of the streams. Light blue is water, dark blue is starch, black is gluten, ad red is energy. The same
scale is used as in Figure 4.
Usually, the concentrated suspension is then directly fed towards a hydrolysis unit, which uses
enzymes to hydrolyse the starch into glucose. The gluten is separated, and dried. There is a fraction
that is not well separated, and which contains part of the starch (the so-called B-type starch). This
fraction is about 20 wt% of the total amount of dry weight of the original wheat flour, and has to
be concentrated and can only be used as cattle feed. As with the corn wet milling process, the flour
fractionation process uses a lot of water and needs a lot of energy to dehydrate and dry the
products. In addition it produces streams which may not be so valuable compared to the primary
products (gluten and starch).
So, is there any way in which we can intensify this process as well? A process developed about 10
years ago in Wageningen6 is now commercialised by Cargill. If you prepare a dough from wheat
flour (using about 40% moisture), and then subject this flour to mild shearing, gluten starts to
aggregate. After a few minutes the aggregates become really large, and when using a concentric
shearing device, it starts to accumulate in the centre of the device.
Figure 3: Subjecting a wheat flour to just shear flow in a curvilinear flow field, leads to spontaneous migration of
the gluten towards the centre of the device. The gluten is of high quality (see right hand picture). The separation
takes place at around 60% solids.
The most important aspect is that no dilution with a large amount of water is necessary: since only
about 45 wt% of moisture is added to the dough, less energy is needed to remove the water again.
Thus the amount of energy used in the separation is much less.
6
S.H. Peighambardoust et al. (2008), J. Cereal Sci., 48(2), 327-338; E.E.J. van der Zalm et al., (2009), J. Food Eng.,
95(4), 572-578
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FPE 31806 – Sustainability in food chains
This illustrates the typical advantage of process intensification: if you don’t add water, you don’t
need to remove it again, and sometimes processes are quicker and more selective. And if you don’t
produce a waste stream, you have no bother with it. However, it is usually difficult to get it working
in practice. The conventional processes work well, and the producers are used to it. Even when a
new principle offers many advantages, it will cost major investments to build such a new plant,
with the risk that in the end such a new system will not perform as expected. Therefore, one sees
a very slow adoption of these types of processes in industry. One may expect however with the
coming increases in energy and food prices that this will change in the coming years.
The main product for the wheat milling process is starch. This is typically produced by suspending
the starch in water (at about 30 – 40 wt% solids), heating by injection of steam, and adding an
enzyme (amylase), which can hydrolyse the starch into smaller fragments (this is called liquefaction
since it renders the suspension of solid starch into a liquid, clear solution). After some time, the
solution is partly cooled, and other enzymes are added (glucoamylase), which break the fragments
down into glucose or lower molecular weight sugars (this is called saccharification).
Vapors
Vapors
Flash
Cooling
Flash Cooling
(vacuum)
Retention
Loop
Retention
(6-9 min) T: 60°C
Steam Loop
DE: 8-15 Steam
(60-90min)
T: 60°C
DE: 52-95 Evaporator
Starch slurry T: 105-110°C
30-35% D.M.+ JetCooker
-amylase T: 95-100°C Vacuum filter
Gelatinisation Saccharification
60°C; 24-48h
Ion-exchange Carbon
colums column
Saccharification
enzyme Finished
product
80% d.s.
Figure 5: The conventional process for enzymatically hydrolysing starch into glucose syrup (used e.g., in soft drinks).
At the end of the process, the excess water has to be evaporated, which again costs a lot of energy.
The amount of water needed can be represented by the size of arrows, which is done in Figure 6.
Evaporation
Evaporation
Steam injection
IX regeneration
Glucose
Starch Maltodextrins Glucose syrup
Figure 6: Diagram of the enzymatic hydrolysis of starch. Once more, the thickness of the arrows indicate the size of
the stream. Water is light blue, green is starch, yellow is the partly hydrolysed starch after liquefaction, and ochre
is glucose syrup7.
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Chapter IV – Sustainable Food Processing
We have shown some time ago that it is possible to do this also in an intensified mode: even at 60
– 75 wt% solids, the liquefaction and saccharification can take place, but one has to be very careful:
the enzymes may be inactivated at too high temperatures or by too intense mixing7. In recent
research it was found one may replace the part where the starch is gelatinised and the part where
the enzymatic hydrolysis takes part, by a single extruder. An extruder is basically a big screw,
rotating in a cylinder. One can push a paste forward in between the screw and the cylinder, and
squeeze it with large force through a small opening at the end. The paste is heated by external
heating and by the friction inside the extruder, and gelatinisation takes place. Concurrent dosing
of enzymes to the mixture will start the enzymatic hydrolysis. Incubation for some time in a holding
vessel will allow for sufficient reaction time.
Vapors
Vapors
Flash
Cooling
Flash Cooling
(vacuum)
Retention
Loop
Retention
(6-9 min) T: 60°C
Steam Loop
DE: 8-15 Steam
(60-90min)
T: 60°C
DE: 52-95 Evaporator
Starch slurry T: 105-110°C
30-35% D.M.+ JetCooker
-amylase T: 95-100°C Vacuum filter
Gelatinisation Saccharification
60°C; 24-48h
Ion-exchange Carbon
colums column
Saccharification
enzyme Finished
product
80% d.s.
Figure 7: The conventional hydrolysis process can be replaced for a major part by a single extruder and a holding
tank. The whole process can then be carried out at much lower water contents; such that no or hardly any water
needs to be evaporated at the end7.
Evaporation
Evaporation
Steam injection
IX regeneration
Figure 8: As Figure 6, but now for the intensified process. Hardly any water needs to be added, and therefore the
energy consumption is reduced dramatically (note that in these figures the energy consumption is not shown) 7.
7M. van der Veen, Towards intensification of starch processing, PhD thesis, Wageningen University, 2005; T. Baks,
Process development for gelatinization and enzymatic hydrolysis of starch at high concentrations, PhD thesis,
Wageningen University, 2007
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FPE 31806 – Sustainability in food chains
Figure 9: Cross-section through a potato (left hand drawing). The bulk of the potato consists of cells (right hand
drawing) that contain a lot of starch granules. Of course the cells contain protein to run their metabolic processes.
In the processing of potatoes (e.g., by the Aardappelen VErwerkend BEdrijf, or Avebe), the
potatoes are crushed and mixed with a lot of water. The diluted suspension then contains starch
granules and other components, which have to be separated. This is done with hydrocyclones,
which use the centrifugal force to isolate the starch granules and leave the other components in
the suspension. Since this is not a very efficient process, one needs to do that six times. The starch
is then filtered on a drum filter, which removes most of the water. The last bit is removed by drying.
The water contains a lot of protein and other components. In the past this water was discarded
(e.g., by Avebe in Groningen to the Waddenzee), and later it was first treated before discarding. In
recent years, it was found that the hydrocyclone process could be omitted, by using the drum filter
itself not only as filter but also as washing system. Simply filtering the (more concentrated)
suspension onto the drum filter, and then washing it a number of times with fresh water leads to
just as high a purity, while the total amount of water consumption was a lot less: 50 to 80 % water
could be saved, which also led to a reduction in energy use of 60%. Interestingly, the whole process
also led to less loss of the starch into the waste water.
3 m3/h 3 m3/h
1 mol/m3 diss. 0.67 mol/m3 diss.
Starch
suspension
3 m3/h 3 m3/h
0.33 mol/m3 diss. 0.67 mol/m3 diss.
Figure 10: Separating starch from mashed potatoes is done with hydrocyclones, in which the starch is suspended
with a lot of water. One needs six such stages to obtain sufficient purity of the starch. A hydrocyclone is basically a
funnel in which the suspension undergoes a swirling flow. The heavier particles swirl outward, and the liquid leaves
at the centre8.
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Chapter IV – Sustainable Food Processing
Figure 11: Left hand diagram: the conventional process used by Avebe for the purification of potato starch. The
multistage hydrocycloning step has been simplified to three stages, in reality there are six. Right hand diagram: the
new process, simpler and saves a lot of water and energy8.
The juice is first mixed with a suspension of calcium hydroxide, which precipitates with multivalent
anions such as sulphate, phosphate, citrate and oxalate and large organic molecules, such as
proteins, saponins and pectins, which aggregate. Next, carbon dioxide is bubbled through the
solution, which precipitates the calcium salts again as calcium carbonate (chalk). The chalk
particles entrap some impurities and absorb others. At the end more carbon dioxide precipitates
more calcium from solution, which is filtered off. At the end, the sugar is crystallized by boiling the
concentrated solution, boiling away the water, which makes the sugar solution supersaturated,
and the sugar crystallises. The interesting thing about this process is that most of the process is
meant to repair the damage that is done at the beginning of the process. The diffusion step
8
Application of Process Intensification in Starch Processing, presentation by M.L.F. Giuseppin, Detmold, 9 April
2003
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FPE 31806 – Sustainability in food chains
(leaching of the cossettes in hot water) degrades and releases many components, which then have
to be removed at some later stage. It would be much more efficient if we could somehow extract
the sugar from the beets, without degrading and extracting other components as well.
This is the aim of a recent development in plant crop processing, called application of pulsed
electric fields (PEF). The cossettes are first made somewhat finer, and then put with some water
into a treatment cell. In the cell, short pulses of an intense electric field are applied. The electric
field causes a polarization of charged ions against the cell membranes, which burst when the force
against them becomes too strong.
The contents of the cell are then released. This happens not at elevated temperatures but at
ambient temperature, which means that most proteins will not be degraded. At the same time,
the cell membranes usually only make small pores, which – when controlled carefully – may only
allow passage of smaller molecules, such as sugar.
There is a number of effects from this PEF treatment. First, less water is needed, which implies
that less water needs to be evaporated at the end. Second, the amount of contaminants in the
juice may be much less. One has to consider that much of the whole sugar refining has to do with
removal of these contaminants and degradation products, and subsequently removing the water
from the sugar. By making the first release more selective and milder (giving less degradation),
most of the removal steps can be omitted or replaced by simpler processes.
After the conventional release by diffusion, the carbonation, liming and clarification removes all
contaminants and degradation products that were created during the high-temperature diffusion
process. When the high-temperature diffusion is replaced by the milder PEF, these steps may be
omitted and replaced by a few membrane steps (mostly ultrafiltration); the evaporation step after
this will be less resource intensive, since less water is added and thus less water needs to be
removed.
9
E. Vorobiev, N. Lebovka (eds.), Electrotechnologies for Extraction from Food Plants and Biomaterials, Springer
Science and Business Media, 2008
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Chapter IV – Sustainable Food Processing
Finally, the pulp will not have been degraded by high temperature, which means that more high-
value components may be isolated from this pulp. This will enable further bio-refining of the raw
material into food products. This is discussed in the next section.
IV.3.5 Conclusions
The traditional ways of processing in the food sector were not efficient, involving a lot of water,
and thus requiring energy-intensive concentration and drying, and generally aimed at producing a
very limited set of products.
The last decades have seen a shift towards intensified processes, requiring less water, combining
process steps to reduce loss, and switch towards milder processes, that limit the degradation
taking place during processing. This then makes many separation and clarification steps
superfluous.
Component $/kg $/L milk % of milk Component $/kg $/L milk % of milk
Milk 0.36 Lactoferrin 300 0.030 8
Butter 5.40 0.22 61 Glycomacropeptide 70 0.110 31
Cheese 4.80 0.48 133 α-lactalbumin 60 0.072 20
Total 0.340 95
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FPE 31806 – Sustainability in food chains
The tables show that milk is valued at about $ 0.35/L (US values; fluctuate in time), and the value
of whey is at $0.06 per litre milk quite low, even after being dehydrated. This is the reason that
whey traditionally was seen as suitable for cattle feed. However, the whey contains a host of
proteins that have value ranging from $20 to $300 per kg, three orders of magnitude more
expensive that the original milk. This represents a significant value to recover from te milk. So, if
we isolate these proteins, we can in total create $0.34 per litre of milk in value, which is 5.7 times
more than the value of the dry whey, and if we add to this $0.34 the co-production of butter and
cheese, we arrive at a total value of $1.04 per litre milk, instead of $0.36 of the original milk – an
increase of 289%.
This can be augmented by creating value from more ingredients, or even converting some lower-
value ingredients into higher valued ones. An example here is the enzymatic conversion of lactose
into galacto-oligosaccharides, an important ingredient in infant formula and a prebiotic. All in all,
this had led to the creation of true dairy refineries.
Conventional
Cream
dairy Lactoferrin
retentate
Lactoperoxidase
Centri- permeate IEX/chroma
Milk
fugation
MF UF tography α-lactalbumin Figure 15
retentate Caseins
β-lactoglobulin A modern dairy, a true milk
IEX/ED
permeate
Immunoglobulins
refinery, converting milk almost
completely into many high-value
Precipi- Curdling ingredients.
Acid Minerals Lactose
tation
In practice factories may be even
Cheese GMP Enzymes more complex, producing more
Filtr./
Caustic
washing
and other products.
Galacto-oligo-
saccharides
Drum
Caseinates
drying
Thus, modern dairy represents the best developed example of food industries not just improving
the efficiency in their processes, but also towards making better use of the complete raw material
by not focusing on a small set of products (cheese, butter, milk), but on the use of all components
in the raw material for high-value food use.
Page IV-14
Chapter IV – Sustainable Food Processing
One of the modern methods to isolate these proteins is by making use of membrane separation.
Since the proteins are the largest molecules, ultrafiltration (preceded by microfiltration to remove
contaminating particulates) can isolate the proteins by diafiltration and subsequent concentration,
followed by drying. This results in an isolate with good properties; however it is not possible to
separate the proteins from each other, and the ultrafiltration membranes are heavily fouled by
the fruit juice.
Another method is the use of isoelectric precipitation. The patatines have an isoelectric point of
4.6 – 5.2, while the isoelectric points of the protease inhibitors range from 5.6 – 6.5. This close
enough to obtain isoelectric precipitation by adjusting the pH to around 5.5. The IEPs are too close
to get any real separation between the proteins, and in fact exacerbates complexation with
phenolic components in the juice, which tend to form complexes with proteins, and are prone to
enzymatic oxidation.
A third method is by using ion exchange, using so-called expanded bed adsorption. Here, use is
made of synthetic resins that contain charged groups. When these groups are positively charged,
such as quaternary amino groups, then these groups will associate with negatively charged
molecules; hence they are called anion exchange resins. When they contain negatively charged
groups, such as −𝑆𝑂32− and −𝐶𝑂𝑂−, then they will associate with positively charged molecules,
hence called cation exchange resins. Beads of these resins will adsorb proteins whenever they are
exposed to a solution of these, depending on the overall charge of the proteins.
+ + +
+ +
+ Figure 16
+ +
Expanded bed adsorption (EBA) for isolation of
+ - -
+
potato proteins. By adjusting the pH it is
+ - -
+-
-
+
+
possible to isolate but also separate different
-
+ - - + proteins (patatine, protease inhibitors) from the
+ - +
- - + +
-
-
+
+ potato fruit juice.
+ + - - - +
- - + +
Depending on the pH of the juice, this can be used to isolate the proteins in the potato fruit juice.
It is even possible to selectively isolate the patatine and the protease inhibitors, by carefully
adjusting the pH. This is done using subsequent anion and cation exchange in expanded beds. Here,
resin particles are lightly fluidized by the feed flow coming from the bottom. This ‘expanded mode’
is used to avoid clogging that would occur in a normal compact bed of resin particles. If the pH is
carefully adjusted, the first column will isolate the anionic proteins, and the second the cationic,
thus separating the patatines from the protease inhibitors. By running at very low temperatures,
it is possible to avoid excessive enzymatic oxidation of the phenolics, which would bind to the
proteins and strongly reduce their value. The isolated proteins have excellent foaming and
Page IV-15
FPE 31806 – Sustainability in food chains
emulsifying properties, are used as gelling agents, and are even considered for use in meat
analogues.
This recovery of proteins from a former waste streams is a good example of the trend towards
‘total use’ of raw materials. Obviously, it forms an important step towards the production of more
food from the same amount of raw materials, which also translates into a strong reduction of
resources per kg of food. It is expected that in the future, more components, such as the smaller
organic acids will also be isolated and marketed as valuable products.
Figure 17
US feed-to-food protein flux into
edible animal products.
63 Mt of feed protein yield edible
animal products containing 4.7
Mt protein, an 8% weighted
mean protein conversion
efficiency.
Source: A Shepon et al., 2016,
Environ, Res. Lett, 11 105002
Even though insects are much more efficient meat producers than warm-blooded animals, they
are still an extra step in the food chain. The ultimate way to produce foods is directly from those
organisms that capture the sunlight and nutrients into their tissues. Replacement of meat by plant
based protein products is therefore potentially the best option.
If we want to make protein foods from plants, we face two major hurdles: the first is the extraction
of the proteins from the plants, and the second is to structure these proteins such, that we can
make nice-tasting products out of it – not necessarily imitations of meat, but at least tasty in their
own right. It is possible to extract a protein rich fraction out of many plants – soy, pea, wheat, etc.,
but this is typically done by milling and dissolving part of the protein in a lot of hot water (compare
the production of sugar). In the production of soy protein isolate (SPI) for example, the proteins in
solution are then often precipitated with acids. The resulting curd is then washed, centrifuged and
10
Weidema B.P., et al., 2008, Environmental improvement potentials of meat and dairy products, Report for the
European Commission Joint Research Centre
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Chapter IV – Sustainable Food Processing
dried. In the production of soy protein concentrate (SPC, different from SPI), the soy flour is
contacted with 20 – 80 % of alcohol at low pH (4 - 5), which will allow dissolution of carbohydrates,
salts and some of the proteins, but the rest of the proteins will stay undissolved. Neutralizing the
residue and drying then results in soy protein concentrate. From the description it may be clear
that once more much dilution, chemicals and salts are used in the production of these types of
components. This does cost a lot of energy and water, produces large quantities of waste and is
therefore not very sustainable. In effect, this leads to the surprising result that while plant proteins
are still more sustainable to produce than meat, they still are quite resource intensive to produce.
This is especially true for isolates, which have been processed more heavily, and they do get into
the range of meat proteins, especially pork. For the less processed concentrates, we see lower
global warming potential, but still not as low as one might expect.
15
2 Plant protein isolates
Figure 18. Global
10
warming potential of
Beef
plant protein isolates
5 Pork, chicken
(upper figure) and
0
concentrates (lower
figure).
Safflower
Hemp
Cottonseed
Mustard
Rapeseed
Lentil
Lupine
Pea
Sesame
Poppy
Soybean
Groundnuts
Linseed
Chickpea
Sunflower
Beans dry
Pigpeon bean
Broadbean
Rapeseed
Wheat
Hemp
Mustard
Potato
Safflower
Broad bean
Quinoa
Lentil
Lupine
Surghum
Sesame
Pea
Cassava
Pearl millet
Triticale
Soybean
Buckwheat
Poppy
Chickory root
Sunflower
Maize
Chickpea
Ginger
Sweet potato
Barley
Oat
Rice
Pigpeon bean
Linseed
Yam
Groundnuts
Beans dry
Source: N. Braconi,
Wageningen Univ. /
Blonk Consultants, 2018
This does not yet include the further processing of these proteins, since they still need to be
assembled into meat analogues. This is generally done by first structuring them into ‘texturised
vegetable proteins’ (TVP) using extrusion, creating a half product that then has to be further
processed into final products. The proteins are suspended into water, and some components are
added, when it is introduced into the extruder. The extruder heats the suspension, and (some of
the) proteins set and crosslink into a solid matrix. By the constant churning of shearing of the
extruder, an internal structure is produced, which comes out in the form of a roughly fibrous
structure. This is the basis of for example the Tivall products.
Another process is the type that is based on the Kweldam patent11. Here, a suspension of the
protein is mixed with alginate, a carbohydrate that does not mix with the proteins, but which
becomes solid with calcium ions. After mixing the alginate with the protein, calcium salt is added,
which solidifies the alginate, which then also fixates the protein phase, with which it is entangled.
Also this process requires a lot of water, just like the production of more or less pure alginate (done
with a lot of chemicals), and calcium salts.
A third process is to use the proteins as nutrient for fungi, which produce fibrillar hyphae.
Harvesting these hyphae then gives a fibrillar product as well. This process, used for production of
Quorn is not very efficient as well: it is done in a lot of water, the conversion of plant protein to
fungal hyphae is not very efficient (a lot is lost) and the whole process requires lengthy post-
processing and repeated heating and cooling.
In the end, most of the half-products are assembled into complete products by (high-moisture)
extrusion, setting, sometimes crosslinking, and hydrating and infusing them with a bouillon and
11
US patent 20050112268
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FPE 31806 – Sustainability in food chains
flavour mixtures. They are then often pasteurised and cooled or frozen for distribution towards
retail.
As discussed, much of the resource efficiency that could be gained by switching to plant proteins
is lost in the isolation or concentration of the plant proteins. Therefore, there is a lot of activity in
finding more resource-efficient alternative ways of processing. There is much attention to ‘gentle’
processing, in which either only low water or no water at all is used. Examples of this way of
processing were already shown in sections IV.3.1 and IV.3.2. Similar technology can be applied for
plant proteins. The raw material may first have to be mechanically dehulled; after that it is milled
into a flour, and either air classification or other methods such as electrostatic separation are used
to separate the flour particles that are rich in protein.
Starch-rich Oil-rich
legume legume
Milling Milling
Suspension Extraction
in water oil Legume
Dry
Protein isolate
Figure 19. A typical conventional wet process for the isolation of plant proteins (left hand diagram) and a dry
route (right hand diagram), which has been proven for cereals such as corn and wheat, legumes and pulses
such as yellow pea, chickpea, faba, soy, lupine, lentils, and for some pseudo-cereals such as quinoa.
Since the process does not involve any water, no drying is required, so it is low in energy use. The
yield (recovery) of the proteins can be quite high, however the purity of the enriched fraction is
not very high at 50 – 75%. This implies that the fractions contain a significant amount of other
materials; mostly fibre. Surprisingly, it was found that these impure fractions were quite suitable
for creation of meat analogues. The hypothesis is that this is because the impurities act as second
phase that enables the creation of an inhomogeneous structure consisting of elongated domains
of one phase, separated by the other phase.
The digestibility of these ‘gently processed’ proteins appears to be quite high and higher than more
heavily processed plant proteins. These proteins are also excellent emulsifiers. Emulsions
stabilised with these proteins (slightly purified using water as sedimentation medium) were much
more stable than emulsions with their conventionally isolated counterparts. This shows that less
purification may not imply poorer performance and in fact may even give better performance in
food products.
Of course, while making protein concentrates, one also makes fractions that are depleted in
proteins. Next to the protein concentrate, a starch concentrate can be created. Also this
concentrate is impure, with for example about 32% fibre for pea starch. However, the thickening
effect that can be achieved in a sauce with such a starch concentrate is just as large as with a
conventionally processed pure starch; even though the concentrate contains app. 30% less starch.
The fibre apparently also contributes to the thickening. Once more, we see that less processing
does noty mean worse performance. And of course, a reduction of the starch content with 30
contributes to a lower calorific value, contributing to a better diet.
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Chapter IV – Sustainable Food Processing
Finally, also oils can be extracted with gentle processing. The conventional oil extraction process
involves mechanical expression followed by extraction with hexane. While this is very efficient, it
denatures the proteins in the soy, while the oils are refined so far that they have to be immediately
supplemented with antioxidants (e.g., tocopherol) to avoid very fast degradation. It produces
however very pure oil. There is however concern with consumers about the use of hexane, which
does leave some residue in the oil, and it is not completely clear whether this fully benign. Also
here, the issue around consumer trust is important. The replacement of hexane by other solvents,
such as supercritical CO2 has not been successful, as this requires capital investments that are very
high, which are not feasible for oils with only moderate value.
Soy Heating
Crushing Flaking Pressing
beans (toasting)
Extraction Evapo-
Swelling Separation Soy oil
ration
Defatten
Heating Drying
ed flakes
Figure 20. Conventional expression and hexane extraction of oils from seeds (e.g., soy).
There is strong and renewed interest in a way to gently extract the oils, which does not denature
proteins and until now still gives lower yields, but avoids the use of hexane on favour of just water,
and may also reduce the necessity of additives. Oils in seeds are located in oleosomes or oil bodies,
vesicles surrounded by a phospholipid membrane also containing a variety of proteins. By blending
the seeds in water and filtering off the solids, one may obtain a dispersion of the intact oil bodies.
This suspension still contains other constituents such as proteins and some carbohydrates, but can
be a quite stable emulsion. Given that many oils are in fact applied in the form of emulsions, one
may consider using these dispersions directly in foods. Recent work showed that the oil bodies can
also be dried into a powder (of a liquid oil), which can be easily redispersed into water.
0.05–3 μm Oleaginous
Water
seeds
Blending
Filtering
Centri- Solids
fugation
Oil bodies
Figure 21. Left: oils in oil seeds are present as oil bodies, protected by a phospholipid membrane that also
contains proteins that protect the oils against premature oxidation. Right: the principle of isolating oil bodies
from a seed (left), by first disrupting the seeds by blending, filtering off the solids, a suspension of intact oil bodies
can be isolated.
The most interesting aspect however is that these intact oil bodies exhibit very high physical and
chemical stability. The proteins in the phosholipid membrane act as natural antioxidant systems,
Page IV-19
FPE 31806 – Sustainability in food chains
Mildly Mildly
refined oil refined oil
Figure 22. Foods can be considered to basically consist of three classes of components: protein, carbohydrates
and lipids. We have nowadays concentrated on the use of (almost) pure ingredients, since this gives us the largest
flexibility in formulation and the best stability in quality. The use of less processed ingredients has the potential
to greatly reduce the environmental impact, but reduces the flexibility in product design. Use of the right gentle
processing will open up sufficient flexibility for food design. Source: J. Jonkman, PhD thesis, Wageningen Univ.,
2018
To obtain the same flexibility in food formulation, one can use these gently processed concentrates,
but to obtain a sufficiently large window for application, one has to use more of these
concentrates. Jonkman showed recently, that instead of the conventional three constituents one
has to use at least 8 gently processed concentrates to substantiate its potential in resource
efficiency.
Therefore the use of less processing may well be the way forward, but it will be at the cost of larger
complexity in food production and design, may possible lead to larger variation in quality of food
products in time, and in fact may also lead to changes in texture and taste. Therefore, this change
will be gradual, and will require consumers to be open to it. Since these intermediates also open
the way towards clean labelling, this may well be the case.
IV.4.4 Conclusions
There has been an important trend away from single-ingredient processing and thinking, towards
complete use of raw materials, by converting these fully into high-value food products. This has
fist happened in the dairy industry, and is now happening with potato and sugar refining. This
makes sure that we make much better use of the raw materials, and reduce the amount of watse
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Chapter IV – Sustainable Food Processing
generated along the chain. The disadvantage is that many of the processes used are still quite
resource intensive.
A second way towards better effectiveness, is by omitting part of the conventional chain in the
production of proteins, by the transition from meat towards plant proteins. This has the potential
for a major improvement in sustainability, but is also hampered by the inefficiency in the isolation
of plant proteins from their raw materials.
The only way to improve this efficiency is to reduce the level of processing. This can be done by
using similar technologies as were already discussed in sections IV.3.1 and IV.3.2, but this has
consequences for the whole chain, as the created ingredients are highly functional, but not pure.
This can be accommodated by producing and employing significantly more of those gently
processed intermediates, which adds to the investment and complexity in the overall food
production chain.
Page IV-21