BIRLA INSTITUTE OF TECHNOLOGY AND SCIENCE, Pilani

Pilani Campus
AUGS/ AGSR Division

SECOND SEMESTER 2018-19

COURSE HANDOUT
Date: 07.01.2019

In addition to part I (General Handout for all courses appended to the Time table) this portion gives further
specific details regarding the course.
Course No : BIO F110
Course Title : BIOLOGY LABORATORY
Instructor-in-Charge : Meghana Tare
Instructor(s) : Team

Tutorial/Practical Instructors: Dr. Uma S. Dubey, Dr.B. Vani, Dr. Shilpi Garg, Dr. Sudeshna Choudhury, Dr.
Sandhya Marathe, Vikram Pareek, Leena Fageria, Neelam Mahala, Heena Saini, Ankita Daiya, Sumukh Thakkar,
Aishwarya Singh, Sonia Narwal.
1. Course Description:
Understanding Biological sciences, analysis and estimation of biomolecules, preparation of temporary slides for
microscopic analysis, study of cell structure and division, investigation of catalytic activity of enzyme, physiology of
plant and animal systems, diversity of living systems.

2. Scope and Objective of the Course:

The major objective of this course is to offer a hands-on experience on fundamental aspects of practical biology. The
student would observe and understand various biological phenomena and also be equipped with some simple
techniques which form the basis of research in biology. The course has been revised to run parallel to General Biology
classes.

3. Text Books:Laboratory Manual for Biology, BITS Pilani, revised, 2019.

4. Reference Books:Simon, E.J. et al: Campbell Essential Biology with Physiology (5th Edition, BITS Pilani custom
edition). Noida: Perason India Education Services Pvt. Ltd., 2015
5. Course Plan:

Module No. Lecture Session/Name Reference Learning Outcome

of the Experiment
Experiment – 1: Scientific Process Lab
Experiment-2: Microscopy Lab
Experiment – 3: Protein Lab: Measurement
of total protein content in
the given sample by
Lowry’s method.
TB/RB Understanding Scientific process, and its components,
learning to design experiments using different
variables while devising a testable hypotheses,
learning to present the results.
TB/RB Learning to handle and use microscope, making
observations using different slides. Permanent/
temporary mounts to understand the relationship
between structure and function.
TB/RB Proteins: the building blocks; their role in humans;
methods to quantify proteins in different samples.

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 BIRLA INSTITUTE OF TECHNOLOGY AND SCIENCE, Pilani
Pilani Campus
AUGS/ AGSR Division

Experiment – 4: Enzyme Lab: TB/RB Properties of an enzyme and chromogenic detection

Tostudytheeffectof the methods.
enzymelactaseonmilk
Experiment – 5: DNA Lab: To extract total TB/RB Basic knowledge about the genetic material, principle
genomic DNA from cells of its isolation.
Experiment – 6: Electrophoresis Lab: TB/RB Understand characterization of DNA based on
Visualizing DNA using gel molecular weight, using electrophoresis.
electrophoresis
Experiment – 7: Transport Lab: TB/RB Effect of tonicity of a solution on different cell types;
Tostudythephenomenon of osmosis & osmoregulation.
plasmolysisinonion peel.
Experiment – 8: Transpiration Lab: TB/RB Understanding the role of stomata in controlling
Preparation of temporary transpiration in plants. Transpiration rate and factors
mount of leaf epidermis to affecting it.
study the structure of
stomata and measurement
of transpiration rate
Experiment – 9: Mitosis Lab: Measurement TB/RB Understanding different phases of cell division,
of mitotic index and different factors affecting it and preparation of slides
duration of mitosis in the to view mitosis in a plant meristematic tissue.
given plant tissue.
Observation of various
stages of mitosis through
permanent slides.
Experiment – Physiology Lab: TB/RB Blood group incompatibility, genetics behind blood
10: Measurement of group inheritance, hemoglobin and its importance;
haemoglobin content in the Blood typing.
human blood and
determination of blood
group and Rh status.
Experiment–11 To study photosynthesis in TB/RB Understanding the process of photosynthesis,
the leaves observing it happen in the lab, importance of different
conditions governing photosynthesis.
6. Evaluation Scheme:

Component Duration Weightage Date & Time Nature of component

(%) (Close Book/ Open Book)
Mid-Semester Test 90 Min. NA NA NA
Comprehensive Examination 3h 40% 30/4 FN CB
Quizzes 30 mins 30% TBA CB
Daily Performance - 30% - NA
(Attendance+ Record)

7. Chamber Consultation Hour:

By appointment over email (meghana.tare@pilani-bits.pilani.ac.in)

8. Notices:
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 BIRLA INSTITUTE OF TECHNOLOGY AND SCIENCE, Pilani
Pilani Campus
AUGS/ AGSR Division

Will be announced on Nalanda and at Biological Sciences display board.

9. Make-up Policy:
No make ups will be granted without prior permission from instructor-in charge. Genuine cases such as
hospitalization, (upon production of the relevant documents as records/ proof) will be considered upon discussion
with team.

10. Note (if any):

The order of experiments listed above may change depending onthe availability of chemicals, enzymes, and other
requirements for a specific experiment.

Instructor-in-charge
Course No. BIO F110