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6 : Determining the concentration of an external solution which is isotonic to the cell sap
of a plant
Aim __________________________________________________________________________
Problem What is the concentration of an external solution which is isotonic to the cell sap?
statement
Hypothesis The concentration of the solution which is isotonic to the cell sap of plant cells is _________
Materials and Potato, tissue papers, distilled water, 0.1 M, 0.2M, 0.3M, 0.4M, 0.5M and 0.6M sucrose
apparatus solutions, scalpel, cork borer, boiling tube, test tube rack, forceps, ruler, 50ml beaker and
electronic balance
Procedure
*draw a graph of percentage difference in the length of potato strips against the
concentration of sucrose solution
Discussion 1. What is the texture and appearance of the potato strips after being immersed in the
various concentrations of sucrose solution?
3. How do you determine the concentration of sucrose solution which is isotonic to the cell
sap of the potato from your graph?
a)
b)
c)
4. Based on the experiment, discuss the process of osmosis in the various concentrations of
sucrose solutions.
a)
b)
c)
Conclusion Based on the graph, the concentration of the solution which is isotonic to the cell sap of
plant cells is ___________________.