Documente Academic
Documente Profesional
Documente Cultură
Presented by
Dilruba Afrin
Course : GEB-407
Dept. of Genetic Engineering & Biotechnology
University of Rajshahi
Contents
Bisubstrate Reactions
sequential or single-
displacement reactions
Ordered sequential
reactions
Random sequential
reactions
ping-pong or double-
displacement reactions
Bisubstrate Reactions
3
Bisubstrate Reactions
Bisubstrate Reactions
Ordered Random
1.sequential or single-
displacement reactions
Sequential reactions are one of the classes involved in
multiple substrate reactions.
In these types of reactions ,all the substrates involved
are bound to the enzyme before catalysis of the
reaction takes place to release the products.
In this reaction, a ternary complex of the enzyme and
both substrates forms.
There are two types of sequential reactions-
Ordered sequential reactions
Random sequential reactions
sequential or single-displacement
reactions
1.Ordered sequential reactions
Figure :
The structures of
creatine and
creatine phosphate,
guanidinium
compounds that are
important in muscle
energy metabolism.
2. ping-pong or double
displacement reactions
Group transfer reactions in which one or more
products are released before all substrates bind
the enzyme.
The defining feature of double-displacement
reactions is the existence of a substituted
enzyme intermediate, in which the enzyme is
temporarily modified.
In this reaction, no ternary complex is formed.
ping-pong reactions
Two stage reaction:
1.A functional group from 1st sub (A) is transferred to the 1st
product (P) forming a stable enzyme (E') –The Ping