Enzyme Quiz

1. Which of the following diagrams correctly shows the reaction pathway of a reaction that absorbs energy?
Circle the correct letter.

2. The energy needed to get a reaction started is referred to as:

a. adhesion energy
b. activation energy
c. cohesion energy
d. chemical energy

3. Which of the following statements is true about catalysts?

a. Catalysts slow down the rate of chemical reactions.
b. All catalysts are enzymes.
c. Catalysts are used up during a chemical reaction.
d. Catalysts lower the activation energy of a chemical reaction.

4. The part of the enzyme where the substrate binds is called the:
a. active site
b. catalyst
c. inhibitor
d. large subunit

5. When a piece of potato is dropped into hydrogen peroxide, the peroxide bubbles vigorously as a
result of what reaction?
a. peroxide being broken into water and oxygen
b. peroxide is destroying germs in the potato
c. more peroxide is being created by the potato
d. potato and peroxide are joining together to make a new protein

PART II – Factors Affecting Enzyme Action

Enzymes work best under certain conditions.

1. Using the data in the chart on the next
page to plot a graph showing the rate of
enzyme action for the enzymes Pepsin
and Trypsin at different varying pH.
pH Rate of Enzyme Rate of Enzyme

Action (for Action (for

Pepsin) Trypsin)

1 1 0

2 3 0

3 7 0

4 3 0

5 1 1

6 0 3

7 0 7

8 0 9

9 0 7

10 0 3

11 0 1

12 0 0

13 0 0

14 0 0
 Name: Date: Period:

2. Considering the range of pH that it is most effective, where in the body might Pepsin be
found?

3. At what pH is Trypsin most effective?

4. Temperature is also an important influence on enzyme action. At what temperature do you think
most human enzymes work best?

5. Sketch a graph of what you think the rate of enzyme action would look like between 0 and 100oC.

Enzyme Rate

0 100

Temperature oC

6. Can you think of any part of the body where an enzyme might work at a slightly different
temperature?

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Part III. Enzyme model

Image modified from Wikimedia Commons (CC BY-SA 3.0)

1. What letter represents the activation energy of the reaction in the presence of enzyme?

2. What letter represents the activation energy of the reaction with no enzyme?

3. What 4 things can affect the way enzymes work? Explain how each thing affects an enzyme.

4. Label the following terms in the following picture

 Enzyme___

 Product(s)___

 Substrate___

 Active site___

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5. The enzyme amylase breakdown the substrate ______________ to produce the
______________.

6. The enzyme sucrase breakdown the substrate ______________ to produce the

______________ + ______________

7. The enzyme lipase works on the substrate called ______________ to produce the
______________.

8. The enzyme lactase works on the substrate called ______________ to produce the
______________.

9. The enzyme pepsin works on the substrate called protein polypeptide to produce the
______________.

10. The enzyme trypsin works on the substrate called protein peptide to produce the
______________.

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 Image modified from Wikimedia Commons (CC BY-SA 3.0)

1. Mark the point on the Y axis that indicates the free energy of the reactants. Green arrow
Mark the point on the Y axis that indicates the free energy of the products. Purple arrow

2. What letter represents the activation energy of the reaction in the presence of enzyme?
A

3. What letter represents the activation energy of the reaction with no enzyme? B

4. Is the reaction exergonic or endergonic? Exergonic

5. Draw a chemical reaction curve of the opposite process (draw an exergonic reaction if
the above is endergonic or draw an endergonic reaction if the above is exergonic).

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