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view. If your estimate is 5 and your field of view 2 mm then the size of
the cell is 2000 µm/5 = 400 µm.
Repeat for each objective
X10
X40
X100
Ocular graticule method: The size of each division on the eyepiece (ocular)
graticule which is located in the eyepiece of the microscope) is calibrated
against the stage micrometer. After the ocular calibration is made for each
objective the eyepiece graticule can then be used as a measure (or measuring
stick) to calculate the size of the object viewed. Calibrating the microscope:
WRITTEN TUTORIAL DUE WEEK 4 during tutorial session.
Name:
ID No:
Tutorial date and time:
Tutor:
Answer questions 1-9
1. Why are microscopes important to the study of biology?
2. Define scale of magnification or magnification of the drawing.
3. Define magnification and resolution so as to distinguish between them.
4. What are the limitations of the light microscope?
5. In a table format describe the function four parts of the microscope (you
can choose any four parts.
6. Identify t least 4 things which can be done when using and storing the
microscope to ensure that the microscope remains in good condition.
7. What is meant by the term par focal as it refers to the microscope or
objective lens. Are the student microscopes in our lab par focal?
8. Describe the field of view method used to calibration of the light
microscope so as to allow for the determination of the size of the object
viewed at each objective lens.
9. Why is it necessary to calibrate at each objective?
10.Is the object changing in size as you go from one objective to the next?
Explain what is happening.