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There are various kinds of materials were found to inhibit the uptake of
glucose by the cell both In Vivo and In Vitro. The glucose uptake
inhibitors are two types.
Abstract
The effects of the turmeric ethyl acetate fraction (TEF) from the
methanolic extract from Curcuma longa L. on lipid metabolism and
underlying mechanisms of lipolysis were investigated in 3T3-L1
adipocytes. The intracellular lipid droplets were stained with Oil red O
dye and quantified. Compared to the control, lipid accumulation was
significantly decreased by 46.6% with treatment by TEF at the
concentration of 20 microg/mL. The intracellular triglyceride (TG) level
was also reduced by 37.9% at the concentration of 20 microg/mL. To
determine the mechanism for TG content reduction, levels of glucose
uptake and glycerol release were measured. Incubation of the 3T3-L1
adipocytes with TEF for 4 hours significantly lowered the cellular level
of glucose in a dose-dependent manner. Furthermore, cellular
expression of insulin-responsive glucose transporter (GLUT)-4 was
decreased by 46%, indicating that reduced glucose uptake was due to
a decrease in cellular GLUT-4 expression. In addition, the level of free
glycerol released into the cultured medium was increased by 36.4%
with the treatment by TEF. In subsequent measurements using
quantitative real-time polymerase chain reaction, mRNA levels of
hormone-sensitive lipase (HSL) and adipose TG lipase (ATGL) were
elevated by 34.8% and 16.9%, respectively, at the concentration of 20
microg/mL. These results suggest that TEF partially inhibits lipogenesis
by the suppression of glucose uptake via the decreased expression of
cellular GLUT-4 and stimulates lipolysis through the induction of HSL
and/or ATGL gene expression, resulting in the increased glycerol
release.
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3. Arsenicals.
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INITIAL STUDY
Step – I. Blood of 2 ml is taken and centrifuse to get plasma and
estimate the Blood glucose immediately. It is (Initial control).
Glucose
Glucose
level
Body wt. in level (final
S.No. Sex Age in yrs (initial
kg. control in
control in
mg/dl)
mg / dl)
P1 FM 54 65 202 154
P2 M 63 70 212 160
P3 M 65 68 223 156
P4 FM 67 66 240 190
P5 M 72 75 261 178
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Discussion :-
Glucose
Glucose
level
Body wt. in level (final
S.No. Sex Age in yrs (initial
kg. control in
control in
mg/dl)
mg / dl)
P1 FM 54 65 154 160
P2 M 63 70 160 165
P3 M 65 68 156 163
P4 FM 67 66 190 198
P5 M 72 75 178 186
Discussion :-
Advantages :
Disadvantages :
Future plan :
Conclusion: