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Extraction of dye from Ixora coccinea and Beta vulgaris for eco-dyeing
Veerabhuvaneshwari Veerichettya*, Baby Shalini Ma, Balaji Sadhasivamaa, Saraswathy
Nachimuthua
aDepartment of Biotechnology, Kumaraguru College of Technology, Coimbatore-641049, Tamil Nadu, India
*Corresponding Author E-mail: veerabhuvaneshwari.v.bt@kct.ac.in
ABSTRACT:
Dyeing is an important application in textiles because; it allows people to wear fabrics of different shades.
Environmental issues of synthetic dyes led to gained customer interest in the natural dyeing sector again. In this
article an effort has been made to use two plant sources as natural dye. Beta vulgaris commonly known as
beetroot or sugar beet has several applications in food sector. Ixora coccinea is generally used as ornamental
plant but it has been found to have several medicinal properties. In this study, extract from the flowers of Ixora
coccinea and extract obtained from the peel of Beta vulgaris using methanol as solvent were analyzed for their
phytochemical properties using TLC. The phytochemical compounds were identified as anthocyanins, phenolic
acids in Ixora coccinea and carotenoids in Beta vulgaris. The extracts were then applied to treated, desized
cotton fabric using various natural and chemical mordants. Based on analysis, the best mordant and technique for
using mordant were determined for dyeing fabric. The fabric was subjected to dyeing and tested for its color
fastness such as rubbing, washing and sunlight to know the best of all, with a view to natural dyeing of fabric
and minimizing synthetic dyes, consequently to save environment.
KEYWORDS: Ixora coccinea, Beta vulgaris, TLC, anthocyanins, phenolic acids, carotenoids, mordants, color
fastness.
INTRODUCTION: Indians are considered as forerunners in the art of natural dyeing. Dyeing is an important
application in textiles because; it allows people to wear fabrics of different shades. Initially, dyes were derived from
naturally available plant materials predominantly leaves, flowers and from certain insect secretions (Cardon (2007)).
The chemicals used for synthetic dyeing are toxic to the environment. Hence, the use of non-toxic, eco-friendly
natural dyes on textiles has become a matter of significant importance due to increased environmental awareness.
Natural dyes find their applications in various sectors such as in the coloring of textiles, cosmetics and drugs
(Gulrajani et al (1992), Deo & Desai et al (1999), Cristea & Vilarem (2006), Chengaiah et al (2010)). Owing to
their non-toxic effects, they are also used in coloring food products (Reddy et al (2005), Korankye (2010)).
Natural dyes produce soothing and soft shades when compared to synthetic dyes. Although indigenous
knowledge system has been practiced over the years in the past, the use of natural dyes has been diminished over
generations due to excessive demand, lack of documentation, precise knowledge on the extraction and dyeing
techniques. Due to these reasons, it has not commercially succeeded like synthetic dyes.
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Fig 1. (A) Flowers of Ixora coccinea (B) Beta vulgaris
2.2 De-sizing
The fabric was de-sized and treated to remove starch if any, as it might hinder the bonding between
mordant, dye and the fabric. For de-sizing, the cloth was initially boiled in soap water for about 30 minutes. Then, it
was washed in a current of warm water and finally in cold water. The fabric was then left to air dry and tested for
dye absorption in all the 3 solvents. The dye extracted using acetone did not fix properly in the fabric which leads to
a conclusion that ethanol and methanol works better in the fabric.
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2.9 Identification of phytochemicals using Thin Layer Chromatography
For Beta vulgaris, the solvent/mobile phase Hexane: Ethyl acetate (8:2) and for Ixora coccinea solvent
mixture containing Hexane: Toluene (9.5:0.5) was efficient to resolve the compounds. The separated spots were
visualized under UV light in order to identify and detect the compounds resolved in the plates.
3.2 Absorbance of the Dye Extracted from Ixora coccinea and Beta vulgaris
The dyes extracted separately from the flowers of Ixora coccinea and from the peel of Beta vulgaris were examined
to spectrophotometer, from 100 to 600 nm and the maximum absorbance of the individual extract was determined.
The maximum absorbance of the dye extracted from Beta vulgaris using methanol as solvent was at 287 nm and for
Ixora coccinea maximum absorbance was at 483 nm (Fig 3) and (Table 1).
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Fig.3. B. Absorption maximum of dye extracted from Ixora coccinea
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Fig.4. Thin layer chromatogram of (A) Beta vulgaris and (B) Ixora coccinea
Simultaneous-mordanting technique was identified to be the most effective for using natural mordants.
Similarly post-mordanting technique was found to be effective for chemical mordants, which is similar to the results
reported by Divya Lekshmi et al., 2013.
After performing the color fastness tests of the dyed fabrics, it was seen that the Ixora coccinea dyed fabric without
the use of any mordants showed very good rubbing fastness (Table 2). Also the fabric dyed with extract from Beta
vulgaris dye using pomegranate rind extract as natural mordant showed very good to excellent washing fastness
(Table 3). On exposure of these fabrics to sunlight, it was observed that the extract of Ixora coccinea along with
pomegranate rind extract as natural mordant and alum as chemical mordant showed good light fastness (Table 4).
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Table 2- Rubbing fastness tests
Sample Change in color Grade Staining Grade
Ixora coccinea 2/3 4/5
(w/o)
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Table 4 - Sunlight fastness test
Sample Day 1 Day 2 Day 3 Day 4 Day 5 Day 6 Day 7
Ixora coccinea ½ ½ ½ 1 1 1 1
(w/o)
Ixora coccinea ¾ ¾ ¾ 3 3 2/3 2/3
(Nm)
Ixora coccinea ¾ ¾ 3 3 2/3 2/3 2
(Cm)
Beta vulgaris ½ ½ 1 1 1 1 1
(w/o)
Beta vulgaris 2/3 2/3 2 2 2 1/2 ½
(Nm)
Beta vulgaris 2 ½ ½ 1 1 1 1
(Cm)
4. CONCLUSION
This study reveals that the pigment extracts obtained from the plant source Beta vulgaris can be of major
use as colorants. TLC (Thin Layer Chromatography) separation of these extracts revealed the presence of various
phytochemicals such as carotenoids, anthocyanins and phenolic acids in the extract. The extracts can hence be used
as natural dyes to provide aesthetic color shades in cotton clothing. On performing the color fastness tests of the
dyed fabrics, it was seen that the Ixora coccinea dyed fabric with pomegranate rind as natural mordant showed very
good rubbing fastness. Also the fabric dyed with extract from Beta vulgaris dye using pomegranate rind extract as
natural mordant showed very good to excellent washing fastness. On exposure of these fabrics to sunlight, it was
observed that the extract of Ixora coccinea along with pomegranate rind extract as natural mordant and alum as
chemical mordant showed good light fastness. On the whole, 100% cotton fabric dyed using the extract obtained
from flowers of Beta vulgaris with the help of pomegranate rind as mordant is the most preferred.
ACKNOWLEDGEMENT:
The authors are grateful to the Dr.R.Shanthi for her support and guiding us in using facilities at Department of
Fashion Technology, Kumaraguru College of Technology.
CONFLICT OF INTEREST:
The authors declare no conflict of interest.
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