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Principle:
An aliquot of the stock solution is reacted with an excess of Na2SO3 (Sodium
Sulfite) followed by back titration with standard H2SO4 (Sulfuric Acid) solution
in the presence of THYMOLPHTHALEIN indicator.
Apparatus:
Burette 50ml, pipette 1ml, 10ml, 20ml, Erlenmeyer flask 250ml.
Checking:
Check that all the glass wares are washed and dried properly. Also check
that all the reagents and reference standards are made properly and the
place of working should be clean.
Environmental Condition:
Cool and dry place.
Reagents:
1. 1M Na2SO3 (prepared by dissolving 126g of anhydrous Na2SO3 in 1 L).
2. Thymolphthalein indicator (10g/l in ethanol).
3. 0.01M H2SO4 (take 0.54g or 1ml of conc. H2SO4 in 1L of DW and
Standardized).
Procedure:
1. Fill the burette with 0.01M H2SO4. Pipette out 50 ml of 1M Na2SO3
in to Erlenmeyer flask. Add two drops of Thymolphthalein
indicator. Add a few drops of 0.01M H2SO4, if necessary until the
blue color disappears.
2. Pipette 10 ml of the stock HCOH solution to the flask (the blue color will
reappear).Titrate the solution with 0.01M H2SO4 until the blue color is
discharged. Record the volume of H2SO4 solution used.
3. Carry out the procedure in duplicate.
Calculation & Results:
RUSSEL GARMENTS
56/1 S.M. MALEH ROAD, OSMAN TOWER, NARAYANGANJ
Calculate the average of the results and use the accurate concentration in the
stock solution (in µg/ml) from the following equation:
Formaldehyde (µg/ml) = vol of H2SO4 (ml) x 0.6 x1000 x Ac.con of H2SO4 (M)
Volume of sample (ml) x Con. of H2SO4 used (0.01M)
Con. of HCHO, ppm or µg/ml = 17.8 x 0.6 x 1000 x 0.0098 / 10 x 0.01 = 1047.
% of HCHO = 37 x 1047 / 1500 = 25.82 %
Precaution:
1. Before starting work with chemicals, must go through the M.S.D.S. of all
the chemicals and follow all the instruction strictly.
2. Wear gloves, goggles, mask, and apron before use of harmful chemicals
and use all the chemicals in fume hood.
a. In case of any damage or harm, stop the working immediately and
inform to supervisor.
1. Preparation of Potassium Hydrogen Phthalate (KHP) soln- 0.01M:
RUSSEL GARMENTS
56/1 S.M. MALEH ROAD, OSMAN TOWER, NARAYANGANJ
Molarity of KHP = W1
Mw x V
Suppose,
Volume of NaOH = 20 ml
Strength of NaOH = 0.0101M (previous find out)
Volume of H2SO4 = 10.3 ml