AMINONIR® For Evo

18/09/2018
AMINONIR®
Ms. Sheila Heidi M. Ramos

Senior Technical Service Manager
Evonik SEA PTE LTD
Validation Results
Customer: PT. Universal Agri Bisnisindo

N = number of samples measured at the host
Achieved N Goal
Parameters Conditions
% 40 %
DM <0.75 85 34 60
0.75-1.25 13 5 30
>1.25 3 1 10
CP <3.0 90 36 80
3-5 10 4 15
>5.0 0 0 5
AA <5 88 424 80
5.0 - 10.0 10 47 15
>10 2 9 5
2
1
18/09/2018
Agenda
Introduction
− AMINONIR®
− AMINOProx®
− AMINONRG®
Importance of grinding
Interpretation of Analytical Data
How to maximize the analytical data
Global Analysis Overview
Comparison of key raw materials
Conclusion
Your Challenges:
• Do we use the most cost-effective raw materials?

• Do we screen and monitor raw materials frequently?
• Do we utilize the most up-to-date assays to control our feed specs?
• Do we use current assay data in our
nutritional matrix?
• Is our data accessible for all decision-
makers?
Amino acid analysis is costly, huge number of samples are required

to be analyzed to capture raw material variation….
4
2
18/09/2018
Importance of evaluating raw material quality
Ingredient quality is the foundation upon which

an animal ration is built.
Establishing an ingredient quality evaluation

program is an essential component of a
successful feed processing plant.
Routine evaluation of incoming feed ingredients

will help insure the adequate use and proper
storage are performed.
Variation is additive and costs money!
The main contributors to nutrient variation and increased safety

margins in compound feed are:
- Raw material variation (batch to batch)
- Inconsistent feed mixing (within batch)
3
18/09/2018
Factors that contribute to raw material variation:
Technical Raw material
Weighing Breed/Variety
Dosing Nutrient Origin
Mixing variation Fertilizer
Transportation Processing
Analytical/Human Storage
factor
Introduction:
- AMINONIR®
- AMINOProx®
- AMINONRG®
4
18/09/2018
NIR Spectroscopy Basics
NIR Spectroscopy Basics
• NIRS = Near InfraRed Spectroscopy

• Non-visible light
• Typical spectral range for feed raw material analysis:
1100 – 2500 nm
Light Prism Sample
Reflection
Detector
10
5
18/09/2018
NIR Calibration Development

Spectra Reference Analysis
Collect spectra and corresponding reference analysis.
There are correlations between spectra and reference values.
Calculation of multiple regression equations.
11
Spectral range for feed parameters
12
6
18/09/2018
NIR Calibration requirements

NIR Calibration requirements
• Calibrations need accurate wet chemistry reference values

• Minimum 100 selected samples needed for a first calibration
version
• At least 30 more samples needed for independent

validations
• Samples must cover a wide range of variation in nutrient

contents, by global origin and different genotypes,
processing conditions
13
AMINOLab®:
The most reliable reference for amino acid analysis
• 55 years of experience in wet chemistry amino acids analysis

• Data basis of more than 50,000 analysed feed ingredients
• Continuous participation at „ring tests“ to check for the precision
and accuracy of our analytical methods (AAFCO, BIPEA, VDLUFA)
AMINOLab® ‒ the ideal basis for accurate NIR-calibrations
14
7
18/09/2018
AMINOLab®
Since 1960 Evonik (former Degussa) supports their customers in the feed
industry with AMINOLab® and AMINOBatch® services for raw material
selection and procurement, quality control, feed formulation and feed mill
management.
Wet Chemical Analysis
− Total amino acid content (Ion Exchange Chromatography)
− Supplemented amino acid content (Ion Exchange Chromatography)
− Total and supplemented Tryptophan (HPLC)
− MHA-FA / MHA-Ca (HPLC)
− Dry Matter (TGA)
− Crude Protein (Dumas)
− 13 Biochrom Amino Acid Analysers (Ion Exchange Chromatography)
− 7 Shimadzu HPLC UV/Fluorescence (HPLC)
− 4 Elementar Nitrogen Analysers
15
AMINOLab®
− 58 years of experience in wet chemistry amino
acids analysis
− Data basis of more than 100.000 analysed feed
ingredients
− Continuous participation at „ring tests“ to check
for the precision
and accuracy of our analytical methods (AAFCO,
BIPEA, VDLUFA)
− Confirmed performance according to stringent
standards leads to EC directives and official US
methods:
• Total content after Oxidation • AOAC 994.12 (1994), EU Commission
and Hydrolysis (Directive 1998, Regulation 2009)
• Supplemented Amino Acids • EU Commission

(Directive 1998, Regulation 2009)
• Lysine, Methionine und Threonine in
trade products and premixes • AOAC 999.13 (1999),
VDLUFA 4.11.6 (1999)
• Tryptophan total content and
supplemented Tryptophan • EU Commission
(Directive 2000, Regulation 2009)
• Methionine in supplemental feed with
high chlorine content • VDLUFA 4.11.5 (1997)
16
8
18/09/2018
AMINONIR®
Prediction Accuracy
17
Validation of Soya Products
Validation with independent samples is the only

way to ensure the accuracy of a NIR calibration
NIR-value (%)
55
Crude protein
50
Argentina
Brazil
USA
45
40 ideal line
35 y = 0.42 + 0.99x R2 = 0.99
30
30 35 40 45 50 55
Lab-value (%)
18 NIRS prediction compared to reference analysis (n = 260)
9
18/09/2018
Validation of Soya Products
NIRS prediction compared to reference analysis (n = 260)
NIR-value (%)
Argentina Methionine
Brazil
USA
ideal line
y = 0.06 + 0.98x R2 = 0.95
Lab-value (%)
19
Evonik AMINONIR® and

AMINOProx® Calibrations
20
10
18/09/2018
AMINONIR® calibration list

Animal by-products Cereal by products
Blood meal Bakery meal
Plasma protein Corn gluten meal
Feather meal Corn products*
Fish meal Noodle by product
Meat meal products Rice bran and polishings
Poultry by-product meal Wheat bran
Poultry meal, feather rich
Pulses
Guar meal
Brewing and Distilling Lupines
Distillers Dried Grains and Solubles Peas
(DDGS), Corn
Distillers Dried Grains and Solubles Milk by-products (includes % lactose)
(DDGS), non-corn
Yeast Milk powder
Whey powder
Cereals
Barley Oil seeds and meal
Corn Coconut meal (Copra)
Millet Cotton seed meal
Oats Mustard meal
Rice Palm kernel meal and expeller
Rye Peanut, Ground meal
Sorghum Rapeseed, Canola meal and expeller
Triticale Sesame
Wheat Soybean hulls
Soybean meal, full fat
Silages Soybean meal and expeller
Alfalfa Soybean protein concentrate
Grass Sunflower meal and expeller
21 Corn by-products (corn bran, corn germ, corn germ meal (de-oiled) , corn gluten feed, corn
hominy feed, corn feed flour)
AMINONIR®
Evonik’s AMINONIR® calibration series to predict nutrients from feed raw

materials by near infrared (NIR) technology
Essential Amino Acids Non-Essential Amino Acids
Methionine Glycine
Cystine Serine
Methionine + Cystine Alanine
Lysine Aspartic Acid
Arginine Glutamic Acid
Isoleucine
Threonine Ammonia (NH3)
Tryptophan Total Amino Acids with NH3
Leucine Total Amino Acids without
Valine NH3
Histidine
Phenylalanine Dry Matter
Crude Protein
22
11
18/09/2018
AMINOProx® Raw Materials and Feed
• Evonik’s calibration series to predict nutrients

from feed raw materials by near infrared (NIR)
technology
• Evonik’s new calibration series to predict nutrients

from poultry and layer feed by near infrared
(NIR) technology
Proximate analysis
(Weende Analysis)
Phosphorus
23
AMINOProx® calibrations
• AMINOProx® reports the following crude nutrients based on

internationally agreed standard methods of extended Weende
Analysis:
− Dry Matter
− Crude Ash
− Crude Protein
− Crude Fat
− Sugars
− Starch
− Crude Fibre
− Neutral Detergent Fibre
− Acid Detergent Fibre
− Phosphorus
− Phytic Phosphorus
24
12
18/09/2018
Equipment used for Proximate Analysis
ICP
25
AMINONRG® Feeds and Raw Materials
• Estimation of energy contents in feed raw

materials and finished poultry and layer feed by
near infrared (NIR) technology
AMINONRG® *:
Swine:
Gross Energy (GE) – Ewan, 1989
Digestible Energy (DE) – Noblet & Perez, 1993
Metabolizable Energy (ME) GP – NRC, 2012
Metabolizable Energy (ME) S – INRA, 2004
Net Energy (NE) – Noblet et al., 1994
Poultry:
Apparent Metabolizable Energy (AMEn) – WPSA, 1989
*AMINONRG® estimates the energy (GE, DE, ME, NE and AMEn) contents of different Feed Raw materials by
using selected regression equations that link the proximate composition predicted by AMINOProx® via Near
Infrared (NIR) spectroscopy.
26
13
18/09/2018
Continue maintaining existing and developing new NIR

calibrations
• Raw material qualities are ever-changing and mandate calibration updates

• With insufficient global supplies and higher prices of standard raw materials
there is a trend towards increased use of alternative ingredients
• It is important that those raw materials are well described in terms of their
quality and variability
This requires NIR calibrations and Evonik is

well positioned to support in that respect
27
Calibration Fact Sheets
Calibration Fact Sheets are

available containing all
information about:
• Version
• Products
• Number of Calibration
Samples
• Collection Time of
Calibration Samples
• Origin of Calibration
Samples
• Calibration Parameters
• Calibration Statistics
28
14
18/09/2018
AMINONIR® Advanced - Outliers
Results are quality checked

− False and incorrect predictions are eliminated
− Results are checked on agreement to AMINONIR®
and AMINOProx® calibration limits
− Results are checked with regard to gH / MD values
− Regular ring tests and comparisons to validate all
NIRS spectrometers in the Evonik network
Enhanced data handling
− PDF-reports and Excel data can be downloaded within
3 minutes
− Amino acid and nutrient contents presented as
needed (standardized DM, as is, AA/CP)
− Data base functionality of „ Analytical Data Advanced”
available online in the Evonik portal
29
AMINONIR® Fact Sheet: Soybean meal
30
15
18/09/2018
AMINOProx® Fact Sheet: Soybean meal
31
AMINONIR® Evonik Lab Network
32
16
18/09/2018
AMINONIR® Customer Network
Europe
181
North America ASIA North

34 228
867 NIR-Spectrometers
use
AMINONIR® calibrations
(January 2018)
Brazil MEA
81 58
ASIA South
190
Spanish-
speaking
America
95
33
AMINONIR® Advanced
Registration of the
Validation of the Upload spectra files
customer instrument
customer’s of a single ingredient
in Evonik’s NIR
instrument in Evonik portal
network
Results can be
Start spectra
downloaded in
processing
Evonik portal
34
17
18/09/2018
AMINONIR® Advanced
Application
35
AMINONIR® Advanced
- Select spectra files
Browse spectra files or

drop them directly from
Windows Explorer
36
18
18/09/2018
AMINONIR® Advanced
- Upload spectra files
Upload spectra files
Either Foss or
Bruker spectra
37
AMINONIR® Advanced
- Check suggestions
38
19
18/09/2018
AMINONIR® Advanced
- Start analysis
39
AMINONIR® Advanced
- Spectra are processed
40
20
18/09/2018
Analytical Data
Advanced 3.0
41
Analytical Data Advanced
Select the Sample Type /

Method
Select individual samples
Download report
42
21
18/09/2018
Influence of
Grinding on NIR
prediction accuracy
43
Influence of Grinding on NIR prediction accuracy
• AMINONIR® is a very quick and accurate method to analyse feed raw materials.
• Compared to wet chemistry the procedure of AMINONIR® is more simple.
• Correct grinding is the only sample preparation needed.
• Because a capable grinder is essential and grinding is time consuming the
importance of grinding often is put into question.
• To quantify and visualize the influence of grinding on NIR prediction accuracy an
extensive grinding test was performed covering all current AMINONIR®
calibrations.
44
22
18/09/2018

• Of each raw material 5 different samples have been taken out of routine AMINONIR®
service
• Samples have been divided into 5 sub-samples for the following grinding procedures:
AMINONIR®
recommended
grinding
Different grinding procedures for a Sunflower Meal
unground Coffee grinder, 15 sec Coffee grinder, 60 sec Retsch ZM200, 1 mm Retsch ZM200, 0.5 mm
45
• All samples were analysed at the Evonik master instrument.
• The optimum grinding is given with 0.5 mm Retsch grinder.
• For all other grinding procedures the relative differences are

shown related to 0.5 mm Retsch grinding.
46
23
18/09/2018
Corn: Relative differences of different grinding vs.

AMINONIR® recommendation
47
Incorrect particle size results in sample outliers
Coffee grinder 15
Raw material Samples Retsch 1 mm Coffee grinder 60 sec
sec
Barley 5 3 5
Corn 5 1 3
DDGS, Non-corn Cereals 5 2 2 2
DDGS, Corn 5 1
Fish Meal 5
Meat Meal 5 1
Peas 2 2 2
Rapeseed Meal and Expeller 10
Rice Bran 5
Rye 5 1 5 5
Sorghum 5 2 5
Soybean Meal and Full Fat 10
Sunflower Meal 5 1 2
Triticale 1 1
Wheat 10 3 10
Wheat Bran 2
Coconut Meal (Copra) 5 5 5 5
Total 90 9 24 41
48
24
18/09/2018
AMINONIR®
Requirements for customer labs to ensure good results
• Capable instrument
• Capable grinder
- Retsch ZM type with

0.5mm ring sieve
- Foss Cyclotec with
1mm screen
49
Grinding tips:
Recommended rotor speed for feed samples: (Retsch ZM200)
Sample Type Speed (rpm)

High fat content 6000
Fatty Samples include animal-based raw materials and

fatty plant-based materials:
Whole Soyabean Meat Meal
Full fat Soybean Meal Meat and Bone Meal
Whole Rapeseed Poulty by-product
Sunflower Seeds Whey
Fish Meal
Fish Feed, pelleted
Sample Type Speed (rpm)

Non-fatty 12000 - 16000
Non-fatty Samples include plant-based raw materials:

Complete/Trials feeds
(Broiler, Pig, Duck, Turkey and Layer etc.)
50
25
18/09/2018
• There is a clear effect of grinding on the AMINONIR® prediction.

• Scale of effect depends on raw material type and analytical parameter.
• For amino acid predictions grinding with a Retsch grinder using 1.0 mm ring sieve results in
differences of approx. 5% compared to a 0.5 mm sieve (reference grinding).
• In general using a coffee grinder results in higher differences than using a Retsch grinder.
• The result of the coffee grinder is significantly influenced by the grinding time and sample
type.
• Raw materials which were already homogenised by a production process (e.g. fish meal,
soya, rape) are less effected by different grinding procedures.
For accurate NIR analysis correct grinding is highly

recommended.
51
Interpretation of Analytical Data
52
26
18/09/2018
Total Amino Acid Contents
• Customer
• Sample description
• Lab code
Raw Material by Wet Chem • Date of delivery / Date of
or AMINONIR® release
• Crude Protein – standardized /
as is
• Dry matter
• Amino Acids total contents -
standardized / in CP / as is
• Sum Amino Acids (without
NH3)
• Ammonia (NH3)
Dry Matter standardized
• Total (including NH3)
information
53
Dry matter standardization
• Data for amino acids and crude protein contents are expressed on a
dry matter basis to eliminate variations in the analytical results due to
moisture
The standard dry matter (DMS) contents include:

• 88% for plant derived raw materials and feeds
• 91% for animal products
• 93% milk products
• Calculation: AAASIS
AADMS = _________ * DMSFeedIngredient
DMASIS
54
27
18/09/2018
Total Amino Acid Contents
• Customer
• Sample description
• Lab code
Raw Material by Wet Chem • Date of delivery / Date of
or AMINONIR® release
• Crude Protein – standardized /
as is
• Dry matter
• Amino Acids total contents -
standardized / in CP / as is
• Sum Amino Acids (without
NH3)
• Ammonia (NH3)
• Total (including NH3)
Crude Protein based
information
55
Crude Protein: Dumas Combustion Method
• This method does not distinguish between protein nitrogen and non-
protein nitrogen
• The CP content is calculated: CP = nitrogen content (%) x CP factor
• CP Factors:
− 6.25 for typical feed ingredients and mixed feeds
− 6.37 for dairy products
− 5.68 for collagen, gelatin
• This number is derived from the fact that the average nitrogen content
of proteins is 16%, thus 6.25 is the reciprocal of 0.16 or 16%
56
28
18/09/2018
Crude Protein: Dumas Combustion Method
• The sum of the analyzed amino acids (wet chemistry: essential and non
essential) is not 100%, compared to crude protein, but mostly 10% or
more below
• Calculation: AAASIS
AAin CP = _________ * 100
CPASIS
• The total amino acid to CP ratio is quite constant within a feedstuff type
• The CP content should not be used as the only criteria for quality
control
• The nitrogen content has its origin not only in amino acids, but also in
non-protein-nitrogen sources (NPN) potentially added to pure protein
57
Ingredient with non-protein byproduct
Raw Material by Wet Chem

or AMINONIR®
Typical CP Level for

Sesame Meal
Sum without NH3 low

(expected 70-90%)
High level of NH3

(expected 2.2-2.5%)
58
29
18/09/2018
CP is not enough as the only criteria for quality control
Meat and bone meal A Meat and bone meal B
59
Standardized ileal digestible contents
• Calculated from Wet Chemical

Raw Material by Wet Chem
and NIR analytical results, by
Std Ileal Dig. Contents multiplication with the
Digestibility Coefficient per
amino acid, per raw material,
per species (poultry, pigs)
• Digestibility coefficients are

published in AMINODat
60
30
18/09/2018
How to make use of the

Analytical Data
61
Quality Purchasing Storage/

control Warehousing
medium quality
What do we do with the information
from the Analytical Data?
Evaluation top quality ????

of quality
low
Suppliers
% Lysine
Evaluation
0,60 0,70 0,80 0,90 1,00 Formulation
1,10 1,20
of Origin
62
31
18/09/2018
Recommendations:
• Increase the number of spectra uploads based on the historical observed variation per raw material
• Standardize the raw material sample identification
Sample name Batch Container number
Sample ID Incoming date PO No.
Origin Supplier Vessel
• Analysis of the raw material data on a regular basis
• Information exchange on the results of data analysis to key persons:
Raw Material Supplier or Origin

Quality Control Feed Formulation Use/Storage Purchasing Evaluation Etc.
• Identification of additional support from Evonik (sampling, data analysis, raw material reporting, etc.)
63
Impact of different
SBM Qualities on
Diet Formulation
64
32
18/09/2018
Average Composition of SBM per Origin, %
Origin DM CP Ash EE CF NDF Sugar AMEn, MJ/kg
Argentina 89.24 47.48 6.55 1.49 3.59 7.64 11.20 10.13
Brazil 88.18 47.12 6.47 2.42 4.89 10.39 9.07 10.04
India 89.31 48.12 7.05 1.65 5.01 11.01 8.01 9.87
USA 88.97 46.67 6.70 2.22 4.08 8.63 10.80 10.20
Origin Lys Met+Cys Thr Trp Val Ile Leu
Argentina 2.93 1.40 1.88 0.65 2.25 2.12 3.57
Brazil 2.88 1.34 1.84 0.63 2.22 2.13 3.57
India 2.99 1.33 1.84 0.63 2.22 2.13 3.62
USA 2.93 1.37 1.84 0.65 2.23 2.11 3.54
65 Evonik - Animal Nutrition
Impact on Diet Formulation and Diet Costs is substantial:

1.10 – 4.93 €/ton
Broiler Starter Composition SBM SBM SBM SBM

Argentina Brazil India USA
SBM, % 37.01 37.36 36.84 37.34
Corn, % 35.34 34.71 35.11 35.06
Wheat, % 20.00 20.00 20.00 20.00
Soybean oil, % 3.42 3.60 3.79 3.57
Vit. + Minerals, % 3.59 3.60 3.59 3.37
MetAMINO®, % 0.275 0.297 0.300 0.283
Biolys®, % 0.266 0.305 0.237 0.257
ThreAMINO®, % 0.054 0.070 0.070 0.064
ValAMINO®, % 0.049 0.063 0.063 0.053
Diet cost*, €/ton 313.90 318.83 318.09 315.00
*Assumption that cost of all SBM is always 401 €/ton.

33
18/09/2018
Impact on Shadow Price
SBM SBM SBM SBM

Argentina Brazil India USA
SBM shadow 401.00 387.82 389.64 398.05
price, €/ton
Differences in the nutritional composition of the

different SBMs should be reflected in a price
difference of up to 13.18 €/ton of SBM!
Soybean meal: comparison of AMINODAT® v5.0

vs. 2017 Indonesia data
AMINODat® v. 5.0 Average:

n CP LYS MET CYS M+C THR TRP ARG ILE LEU VAL HIS PHE
Soybean meal 1241 Mean 46.47 2.81 0.62 0.66 1.28 1.80 0.62 3.38 2.11 3.53 2.20 1.21 2.36
2017 Indonesia Average:

n CP LYS MET CYS M+C THR TRP ARG ILE LEU VAL HIS PHE
Mean 47.26 2.92 0.63 0.67 1.30 1.84 0.63 3.48 2.18 3.62 2.26 1.24 2.43
SD 1.50 0.09 0.02 0.03 0.04 0.05 0.02 0.11 0.08 0.11 0.07 0.03 0.09
Soybean meal 8246 CV 3.17 3.15 3.16 3.80 3.40 2.64 3.13 3.28 3.63 3.08 3.05 2.79 3.72
Min 34.17 2.17 0.47 0.49 1.01 1.34 0.46 2.55 1.56 2.59 1.62 0.90 1.73
Max 51.49 3.23 0.68 0.73 1.41 1.97 0.70 3.86 2.49 3.99 2.49 1.35 2.82
68
34
18/09/2018
Impact on Feed Cost
Cost per kg: IDR 4,897 Cost per kg: IDR 4867
Difference: IDR 30/kg

≈ USD 2.22/MT of Finished Feed
If a feed producer is manufacturing 100,000MT of broiler feeds a month the cost savings on using
actual values for Corn and SBM alone in this example would amount to USD 222,000 annually
69
Stringent Quality Control will identify Sub-

populations
Example: Wheat – Different Crude Protein Content according to its Origin
70
35
18/09/2018
Histogram for Crude Protein in Wheat samples of

different Origin
Origin A Origin B
Histograms for selected Amino Acids for Wheat samples

from 2016
Origin A Origin B
36
18/09/2018
Average Composition of the Wheat Samples, %
Origin DM CP Ash EE CF ADF NDF Starch AME,

MJ/kg
A 89.35 10.51 1.62 1.97 2.24 3.05 11.48 60.27 13.20
B 90.07 13.50 1.80 2.06 2.26 3.08 11.85 59.73 13.26
Origin Lys Met + Cys Thr Trp Val Ile Leu
A 0.31 0.42 0.31 0.14 0.46 0.35 0.69
B 0.37 0.51 0.38 0.16 0.57 0.45 0.88
Impact on Diet Formulation and Diet Costs is substantial: 9.39 €/ton
Diet Composition: Wheat A Wheat B

Broiler Finisher Feed
Wheat, % 52.39 55.99
SBM, % 28.37 25.27
Corn, % 10.00 10.00
Soybean oil, % 5.97 5.40
Vit. + Minerals, % 2.78 2.81
MetAMINO®, % 0.22 0.19
Biolys®, % 0.22 0.29
ThreAMINO®, % 0.05 0.05
Diet cost*, €/ton 275.60 266.21
*Assumption: Wheat 168.00 €/ton; SBM 354.00 €/ton; Corn 164.00 €/ton; Soybean oil 761€/ton;
37
18/09/2018
Impact on Shadow Price
Price of Wheat B: 168.00 €/ton
Competitive Price of Wheat A: 150.08 €/ton
Differences in the nutritional composition of the two

different Wheat Qualities should be reflected in a price
difference of 17.92 €/ton of Wheat!
Raw material storage based on nutrient content
76
38
18/09/2018
Scatter-grams show a distinct difference in AA content in

CP depending on the origin: Soybean meal
77
Economics of using AMINONIR® to control variability

200,000 MT feed production/year
Typical ration: 60% Corn, 30% Soybean meal, 5% Rice bran, & 5% others
Needs per year:
120 KT Corn
60 KT Soybean meal
10 KT Rice bran
QC sampling program:
Corn
Soybean meal
Ricebran
78
39
18/09/2018
Economics of using AMINONIR® to control variability
Total samples/year based on actual:

Corn : 1200
SBM: 1800
Ricebran: 500
Analytical costs:
If analyzing for crude protein and then using regression equations for amino
acid values:
$280,000/year
If analyzing via wet chemistry:
$1.05 million/year
79
Global Analysis Overview
80
40
18/09/2018
AMINONIR®
Allocation of samples 2017
AMINONIR®-Countries with more than 150 uploaded spectra are listed
Brazil
USA
Brazil
India
China
Mexico
Ecuador USA Pakistan
Chile South Africa
Ukraine
Argentina
Bolivia
Indonesia
Russia
Jamaica
Australia
Germany India Germany
Australia Russia
Bolivia
Jamaica China Ukraine
Indonesia Chile
Ecuador
Argentina Spain
Philippines
Mexico Peru
South Africa Mexico Hungary
Pakistan Vietnam
81
AMINONIR® Advanced
Online NIR prediction via Internet
900000
850000 AMINONIR®-Spectra
800000 (predicted manually in Hanau)
750000
700000
650000 AMINONIR®-Advanced
600000 (fully automated application)
550000
500000
450000
400000
350000
300000
250000
200000
150000
100000
50000
0
2006 2007 2008 2009 2010 2011 2012 2013 2014 2015 2016 2017
A total of 869,840 (2016: 677,711) spectra of ingredients, ground and scanned

with NIRS in customer labs were analysed 2017 on their amino acid contents.
82
41
18/09/2018
2017 Asia South top 20 customer uploads

30000
26771 55% ( 221,590) of

spectra uploads
25000
Average: ≈ 2,000
20000
15000
10000 9202
8507 8287 8191
6576
5908
4969 4880 4724
5000 4142 4025 3681 3674
3652 3630 3584 3484 3343
83
AMINONIR® ̶ Hanau lab
Types with 100 and more analyzed samples are listed

Peas
Soya Expeller Soybean Meal
Rapeseed Meal
Corn
Meat & Bone meal
Wheat Bran Wheat
Poultry meal Barley
Meat Meal
Sorghum Sunflower Meal
Soybean Full Fat
Soya, full fat DDGS Corn Poultry Byproduct Meal
Meat and Bone Meal
Soybean Expeller
Sunflower Peas
meal Rapeseed Meal
Wheat Bran
Meat Meal
Sorghum
Soybean DDGS, Corn
Barley meal Blood Meal
Cottonseed Meal
Mixture Animal Protein Blend
Triticale
Feather Meal
Wheat Corn Coconut Meal (Copra)
Fish Meal
84
42
18/09/2018
AMINONIR® ̶ Singapore lab
Types with 10 and more analyzed samples are listed

Soybean Meal
Ricebran
Corngluten meal Corn
Poultry meal Meat and Bone Meal
Fish meal Fish Meal
DDGS, Corn
Corn Gluten Meal
Rice Bran/Polishings
Poultry Byproduct Meal
MBM Rapeseed Meal
DDGS, Corn
Rice Bran, Deoiled
Meat Meal
Coconut Meal (Copra)
Wheat
Corn
Wheat Bran
Soybean Full Fat
Cottonseed Meal
Wheat Feed Flour
Rice, Broken
Blood Meal
Soybean meal Feather Meal
85
What is the current practice?

− How do we collect our samples?
− How may samples do we collect per truck load? Per container? Per bag?
− What sampling rules/patterns do we follow?
− How do we divide our samples?
− Where do we store our samples?
− How long do we need to store our samples?
86
43
18/09/2018
Spectra information
Sample name
Sample code
Country of Origin (Imported or Local)
Supplier (trader)
Delivery date
Truck number
Analyst
P/O Number
Lot no.
…..
Information required varies, depending on what is important to the customer.
87
Pre-requisites for Collaboration with Evonik‘s NIR

Services
• Air-conditioned lab
• Experienced and reliable person who maintains
the NIR instrument and runs the analytical service
• Retsch Grinder ZM 200 (0.5 mm sieve)
• Suitable NIR instrument
• NIR instrument connected with internet
• Ongoing assessment of the analytical results
according supplier, origin or processing plant
• Standardization of the customer instrument will be
done by one of our regional NIR experts
44
18/09/2018
Comparison of key raw

materials
89
Corn: Amino Acids Profile
ESSENTIAL AMINO ACIDS

Region n CP
LYS MET CYS M+C THR TRP ARG ILE LEU VAL HIS PHE
Mean 8.04 0.25 0.17 0.18 0.35 0.29 0.06 0.38 0.27 0.98 0.39 0.24 0.39
SEA 13568
CV 7.50 7.02 7.25 6.50 6.63 7.18 5.32 8.28 8.15 9.11 6.66 6.81 8.93
Mean 8.25 0.25 0.17 0.18 0.36 0.30 0.06 0.40 0.28 0.97 0.39 0.24 0.40
India 9418
CV 6.15 5.67 6.40 4.92 5.20 6.05 4.57 5.79 6.85 8.32 5.87 6.14 7.52
Mean 7.52 0.24 0.15 0.17 0.32 0.27 0.06 0.36 0.36 0.91 0.36 0.22 0.37
USA 475
CV 7.31 4.17 6.67 5.88 6.25 7.41 4.71 5.56 7.69 8.79 5.56 4.55 8.11
Mean 7.80 0.23 0.16 0.18 0.34 0.28 0.06 0.37 0.27 0.95 0.37 0.23 0.38
Brazil 9515
CV 4.61 5.10 5.79 3.99 4.35 4.51 3.71 4.90 5.25 6.68 4.47 4.56 5.89
Mean 7.21 0.24 0.14 0.16 0.30 0.26 0.06 0.37 0.24 0.81 0.34 0.21 0.33
Argentina 294
CV 8.30 9.70 7.30 8.10 6.70 6.00 8.60 11.80 14.80 9.80 10.20 13.50 8.30
Poultry SIDC 91 95 89 92 89 83 89 98 93 95 97 93
Swine SIDC 75 87 83 85 80 77 89 86 89 85 87 87
Crop report, 2017
90
45
18/09/2018
DDGS Corn: Amino Acids Profile

Region n CP
Mean 27.67 0.81 0.53 0.51 1.03 1.02 0.22 1.20 0.99 3.10 1.31 0.72 1.32
SEA 7935
CV 4.86 7.30 6.77 6.23 5.93 5.07 6.27 5.91 6.10 6.82 5.30 5.23 6.33
Mean 37.73 1.11 0.87 0.72 1.57 1.35 0.41 2.24 1.47 3.25 1.98 0.85 1.86
India 74
CV 23.51 29.50 31.14 25.45 30.61 22.55 40.47 41.16 28.78 11.87 29.13 18.20 25.43
Mean 27.12 0.79 0.52 0.51 1.02 1.00 0.22 1.17 0.97 3.00 1.27 0.70 1.28
USA 2667
CV 6.12 7.59 9.62 8.82 8.82 6.00 9.09 9.40 7.22 7.00 7.09 7.14 7.81
Poultry SIDC 65 86 82 85 72 81 82 80 86 78 74 80
Swine SIDC 63 83 75 76 72 77 81 78 85 77 79 82
Crop report, 2017
91
SBM: Amino Acids Profile

Region n CP
Mean 47.21 2.92 0.63 0.67 1.30 1.84 0.64 3.46 2.18 3.61 2.26 1.24 2.42
SEA 14237
CV 2.90 3.13 2.72 3.58 3.02 2.43 2.88 3.21 3.49 2.93 2.88 2.59 3.58
Mean 47.14 2.88 0.61 0.64 1.26 1.80 0.62 3.48 2.16 3.58 2.22 1.23 2.41
India 10686
CV 1.72 2.35 2.45 4.92 2.70 2.22 2.57 2.14 2.17 2.09 2.17 2.26 2.08
Mean 46.12 2.87 0.62 0.67 1.30 1.79 0.64 3.38 2.11 3.50 2.21 1.20 2.32
USA 253
CV 1.41 1.39 1.61 1.49 1.54 1.12 1.56 1.48 1.42 1.43 1.36 1.67 1.72
Brazil Mean 46.50 2.87 0.62 0.67 1.28 1.81 0.63 3.39 2.14 3.56 2.22 1.21 2.38
(46-47% 8814
CP) CV 0.60 1.89 1.57 1.93 1.63 1.27 1.34 1.23 0.92 1.14 0.96 1.47 1.12
Mean 45.38 2.79 0.60 0.64 1.25 1.77 0.61 3.28 2.08 3.47 2.18 1.19 2.30
Argentina 158
CV 1.60 2.00 2.20 2.80 2.80 1.60 1.80 1.80 2.00 1.70 1.80 1.80 1.90
Poultry SIDC 89 90 79 84 83 89 92 87 88 87 90 89
Swine SIDC 90 92 85 88 88 89 95 90 89 90 91 90
Crop report, 2017
92
46
18/09/2018
Wheat: Amino Acids Profile

Region n CP
Mean 10.89 0.31 0.17 0.25 0.42 0.31 0.14 0.52 0.36 0.71 0.46 0.25 0.49
SEA 8978
CV 8.00 8.55 7.90 8.79 7.92 8.12 6.57 9.74 8.16 8.08 7.51 7.85 10.23
Mean 11.92 0.31 0.19 0.27 0.44 0.33 0.14 0.52 0.40 0.79 0.49 0.26 0.56
India 47
CV 4.63 13.54 5.27 6.24 5.62 6.67 8.83 12.10 5.05 4.78 6.32 6.40 6.00
Mean 11.26 0.31 0.25 0.17 0.42 0.30 0.14 0.53 0.37 0.72 0.47 0.26 0.48
Ukraine
CV 10 8.23 7.69 7.92 7.04 8.50 6.80 9.10 9.68 9.23 8.86 9.12 10.52
Mean 11.95 0.35 0.19 0.27 0.45 0.33 0.15 0.58 0.40 0.78 0.51 0.28 0.51
Argentina 187
CV 12.30 15.50 12.70 10.30 11.00 15.10 10.70 18.30 13.00 12.60 14.40 13.70 13.50
Poultry
86 91 91 92 88 86 86 94 91 91 90 91
SIDC
Swine SIDC 83 89 89 89 86 88 92 90 90 88 91 91
Crop report, 2017
93
SBM – Jan to May 2018 – South East Asia Data
2696 1279
2308
8342
USA Argentina India Brazil
94
47
18/09/2018
SBM sources 2018 – Jan to May- Proximate composition

Proximate composition
AA n CP EE Fiber NDF ADF P Phytic

P
USA 1279 47.25 2.17 4.01 10.35 6.17 5980 3588
Argentina 2308 47.04 2.09 3.42 9.84 5.40 6242 3745
India 8342 47.97 1.87 6.02 13.76 8.09 5217 3130
Brazil 2696 48.81 2.33 3.63 9.99 5.73 6093 3656
CP EE Fiber ADF NDF

Crop report 2017
47.21 2.14 3.58 5.81 9.93
95
SBM sources 2018 – Jan to May- Proximate composition
Starch Sugar
0.8 0.71 0.74 0.69 12
0.6 10.03 9.95
10 8.82
0.6
8 6.79
0.4 6
0.2 4
0 2
0
Starch Sugar
Crop report 2017
0.76 9.07
96
48
18/09/2018
SBM sources 2018 – Amino acid composition
Total AA
AA n Lys Met Cys Thr Trp Arg Ile Val
USA 1279 2.90 0.63 0.68 1.83 0.64 3.43 2.14 2.24
Argentina 2308 2.88 0.62 0.66 1.83 0.63 3.41 2.14 2.24
India 8342 2.89 0.61 0.65 1.82 0.63 3.52 2.17 2.24
Brazil 2696 2.97 0.64 0.69 1.88 0.65 3.55 2.24 2.32
Lys Met Cys Thr Trp Val

Crop report 2017
2.92 0.63 0.67 1.84 0.64 2.26
97
SBM sources 2018 – Energy
AA n NE Growing NE sows AME

pig poultry
USA 1279 2170 2334 2366
Argentina 2308 2167 2329 2355
India 8342 2023 2177 2337
Brazil 2696 2174 2339 2402
NE growing pigs NE sows AME

Crop report 2017
2135 2301 2343
98
49
18/09/2018
Conclusions
99
Benefits for the customer
• All Raw Material Calibrations available for Amino AMINONIR®

Acids, Proximate and Energy Parameters AMINOProx®
AMINONRG®
• Amino acids, Proximate and Energy from the same
sample combine
• Quality assurance through regular ring tests and sample
performance trials processing
speed with
• No lengthy and expensive wet chemical analyses of prediction
Weende parameters
accuracy
100
50
18/09/2018
Proper raw material monitoring and evaluation of results

brings many benefits
• Raw material purchase Reliable basis for

& management separate pricing &
storage according to
nutritional value
• Feed formulation Economic use of different
qualities in least cost
formulation
Reduced diet cost due
to lower safety margin
on protein and amino
acids
• Live production More consistent

& slaughter performance
= Higher overall profitability

101
Evonik - Turning knowledge into your profit
102
51
18/09/2018
103
52

AMINONIR® For Evo

Încărcat de

Informații document

Titlu original

Drepturi de autor

Formate disponibile

Partajați acest document

Partajați sau inserați document

Opțiuni de partajare

Vi se pare util acest document?

Este necorespunzător acest conținut?

Drepturi de autor:

Formate disponibile

AMINONIR® For Evo

Încărcat de

Drepturi de autor:

Formate disponibile

18/09/2018

Ms. Sheila Heidi M. Ramos

Customer: PT. Universal Agri Bisnisindo

• Do we use the most cost-effective raw materials?

Amino acid analysis is costly, huge number of samples are required

Importance of evaluating raw material quality

Ingredient quality is the foundation upon which

Establishing an ingredient quality evaluation

Routine evaluation of incoming feed ingredients

Variation is additive and costs money!

The main contributors to nutrient variation and increased safety

- Raw material variation (batch to batch)

- Inconsistent feed mixing (within batch)

Factors that contribute to raw material variation:

Technical Raw material

Dosing Nutrient Origin

Mixing variation Fertilizer

NIR Spectroscopy Basics

NIR Spectroscopy Basics

• NIRS = Near InfraRed Spectroscopy

Light Prism Sample

NIR Calibration Development

Collect spectra and corresponding reference analysis.

There are correlations between spectra and reference values.

Calculation of multiple regression equations.

Spectral range for feed parameters

NIR Calibration requirements

• Calibrations need accurate wet chemistry reference values

• At least 30 more samples needed for independent

• Samples must cover a wide range of variation in nutrient

• 55 years of experience in wet chemistry amino acids analysis

AMINOLab® ‒ the ideal basis for accurate NIR-calibrations

Wet Chemical Analysis

− Total amino acid content (Ion Exchange Chromatography)

− Supplemented amino acid content (Ion Exchange Chromatography)

− Total and supplemented Tryptophan (HPLC)

− MHA-FA / MHA-Ca (HPLC)

− Dry Matter (TGA)

− Crude Protein (Dumas)

− 13 Biochrom Amino Acid Analysers (Ion Exchange Chromatography)

− 7 Shimadzu HPLC UV/Fluorescence (HPLC)

− 4 Elementar Nitrogen Analysers

• Supplemented Amino Acids • EU Commission

Validation of Soya Products

Validation with independent samples is the only

35 y = 0.42 + 0.99x R2 = 0.99

18 NIRS prediction compared to reference analysis (n = 260)

Validation of Soya Products

NIRS prediction compared to reference analysis (n = 260)

y = 0.06 + 0.98x R2 = 0.95

Evonik AMINONIR® and

AMINONIR® calibration list

Evonik’s AMINONIR® calibration series to predict nutrients from feed raw

AMINOProx® Raw Materials and Feed

• Evonik’s calibration series to predict nutrients

• Evonik’s new calibration series to predict nutrients

• AMINOProx® reports the following crude nutrients based on

Equipment used for Proximate Analysis

AMINONRG® Feeds and Raw Materials

• Estimation of energy contents in feed raw

Continue maintaining existing and developing new NIR

• Raw material qualities are ever-changing and mandate calibration updates

This requires NIR calibrations and Evonik is