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TECHNICAL SPECIFICATIONS

SHIMADZU PROMINENCE LC-20A MODULAR HPLC SYSTEM

Grupo de Ingeniería y Biotecnología Ambiental ● www.eula.cl/giba ● giba@udec.cl ● 41 2204067 – 41 2661033


Shimadzu LC-20A System with UV-VIS Detector

System Controller
 Remotely monitor instrument status using a
portable terminal.
 Overlapping injection function results in
shorter analysis times carryover.
Pump
Check valve improvements provide more
stable solvent delivery.
UV- Vis Detector
Offer an exceptional level of
Quaternary gradient unit sensitivity and stability.
Stronger support for buffer solutions with high
salt concentrations.

Column oven
Autosampler  Improved leakage detection (leak sensor).
Multi-rinse function (max. two solutions) achieves  Improved separation in simultaneous analysis of
even lower carryover. multiple components by simultaneous control of up
to four units.

 Wide Variety of Application Systems Available

Several application-specific systems are available that take advantage of the remarkable expandability of Prominence
systems.

Foods: Chemical Industry:


- Food components - Plastics
- Additives - Solvents
- Residual pesticides - Paints
- Fragrances - Fiber and paper

Environment: Pharmaceuticals:
- Atmosphere - Drug ingredients
- Drinking water - Antibiotics and antimicrobials
- Soil - Herbal medicines
- Biofuels - natural products
- Veterinary pharmaceuticals

Grupo de Ingeniería y Biotecnología Ambiental ● www.eula.cl/giba ● giba@udec.cl ● 41 2204067 – 41 2661033


Shimadzu 20A LC System with UV-VIS Detector is currently used in a wide variety of fields. Higher reliability in analysis
data and higher efficiency in total analysis workflow are required for faster development of new drugs, for better food
safety, and for meeting higher standards in environmental regulations. The system has Shimadzu Lab Solutions software
for LC Data System.

1. LC-20AT Solvent Delivery Module

Description Specification
Pump type Double reciprocating plunger pump
Pumping methods Constant flow delivery and constant pressure delivery
Flow rate setting range 0.001 – 5 mL/min (1.0 – 40 MPa) 5.001 – 10 mL/min (1.0 – 20 MPa)
Flow rate accuracy ± 2% or ± 2 µL/min, whichever is greater (when water is pumped at 0.1 – 5 mL/min, 1
- 40 MPa, with water and room temperature constant between 20 - 30°C)
Flow rate precision ± 0.3% (RSD 0.1% and below) (when water is pumped at 0.1 - 5 mL/min, 1 - 40 MPa,
with water and room temperature constant between 20 - 30°C)
Pressure setting range 1.0 – 40 MPa (0.1 MPa steps)
Pressure accuracy ± 10% or 1.0 MPa, whichever is greater
Pressure limits function Upper and lower limit
Wetted surface materials SUS316L, PEEK, PTFE, ruby, sapphire, HastelloyC®
Gradient system Quaternary in Low pressure
Gradient profile Step, linear and exponential functions (only when LC workstation controls) possible at
multiple levels
Maximum program steps Max. 400 steps per program, 20 program files

2. SPD-20 AV- UV-Vis Detector

Description Specification
Light source Deuterium (D2) lamp, tungsten (W) lamp.
Wavelength range 190 to 900 nm.
Bandwidth, slit width 8 nm.
Wavelength accuracy 1 nm max.
Wavelength precision 0.1 nm max.
Noise 0.5 X 10-5 AU (under specified conditions).
Drift 1 X 10-4 AU/h (under specified conditions).
Linearity 2.5 AU (ASTM standard).
Dual-wavelength detection in the range 190 to 370 nm and upwards of 371, ratio-
Functions
chromatogram output, wavelength scanning.
Cell Path length: 10 mm Volume: 12µL Pressure: 12 MPa.
Cell temperature-control range 5 ºC above room temperature to 50 ºC
Operating temperature range 4 ºC to 35 ºC (non-condensing atmosphere)

3. DGU-205 Degassing unit

Description Specification
"On line" five channels and vacuum filtration membrane for five channels solvents (four
Type
mobile phase and a fifth for solvent washing autosampler)

Grupo de Ingeniería y Biotecnología Ambiental ● www.eula.cl/giba ● giba@udec.cl ● 41 2204067 – 41 2661033


4. CTO-20AC Column Oven

Description Specification
Temperature-control method Forced-air circulation
Cooling method Electronic cooling
Temperature-setting range 4 ºC to 85 ºC
Temperature-control precision 0.1 ºC max.
Temperature-control range 10 ºC below room temperature to 85 ºC
Storage capacity 10 columns, max 25 cm length
Safety measures Solvent sensor, temperature fuse, temperature upper limit
Operating temperature range 4 ºC to 35 ºC

5. CBM-20A HPLC System Controller

Description Specification
Communication type (INPUT) Optical fiber
Type Communication
Communication by ETHERNET
(OUTPUT), Controller-PC
External control Direct PC or connection through WEB
Units can control 8 remote connectors (up to 12 with optional); manual injector input and error.

6. SIL-20AC Autosampler SIL-20AC

Description Specification
Injection Method Metering pump, variable volume, no sample loss
Injection Volume 0.1 to 100 µL (std), 1 to 2,000 µL (opt)
Sample Capacity 70 1.5 mL vials (incl. std.); Control Rack: 10 1.5 mL vials
Injection Volume Accuracy + 1% (50µL, n=10)
Injection Volume Precision RSD < 0.3% (10µL)
Carryover 0.005% (Chlorhexidine)
Sample Aspiration Rate 0.1-15 µL/sec (0.1µL steps)
Rinsing Rate 1-35 µL/sec (1µL steps)
Rinsing Options Before Aspiration, After Aspiration, Before and After Aspiration, or None (Optional rinse
pump for multiple solvent wash available)
Temperature Control 4o -40o C w/ built-in dehumidifier
pH Range 1 to 14
Operating Temperature 4o C to 35o C (non-condensing atmosphere)

Grupo de Ingeniería y Biotecnología Ambiental ● www.eula.cl/giba ● giba@udec.cl ● 41 2204067 – 41 2661033

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