A59439 AA
July 2008
Combination Calomel pHree Electrodes
A51705 12mm x 150mm epoxy sealed
A51706 12mm x 150mm glass sealed
A51707 12mm x 150mm epoxy refillable
A51708 12mm x 150mm glass refillable
A51709 9.5mm x 300mm epoxy sealed
A51710 9.5mm x 300mm glass sealed
A51711 6mm x 150mm epoxy sealed
A51712 6mm x 150mm glass sealed
Combination Ag/AgCI Electrodes
A57177 12mm x 150mm epoxy sealed
A57186 12mm x 150mm epoxy refillable
A57178 12mm x 150mm double-junction epoxy
sealed
A57184 12mm x 150mm double-junction flat bulb
epoxy sealed
A57185 9.5mm x 150mm double-junction
spear-tipped epoxy sealed
A57181 12mm x 150mm glass refillable
A57179 9.5mm x 300mm epoxy sealed
A57182 9.5mm x 300mm glass refillable
A57180 6mm x 150mm epoxy sealed
A57187 6mm x 150mm epoxy refillable
A57183 6mm x 150mm glass refillable
pH and Reference Electrodes
A57188 pH measuring half cell, epoxy
A57189 pH measuring half cell, glass
A57190 Calomel pHree reference only, epoxy
sealed
A57192 Calomel pHree reference only, glass
refillable
A57193 Ag/AgCI reference only, epoxy sealed
A57194 Ag/AgCI reference only, glass refillable
Combination Low Ionic Strength Electrodes
A57195 12mm x 150mm epoxy refillable
A57196 12mm x 150mm glass refillable
Combination pHRESH Research-Grade
Electrodes
511110 12mm x 150mm epoxy refillable
511111 12mm x 150mm glass refillable
4 4300 North Harbor Boulevard, Fullerton, CA 92834-3100
pH/Reference/ATC (3-in-1) Electrodes
511052 12mm x 150mm 3-in-1 electrode for pHi
series 2xx, 3xx, 6xx meters
511053 12mm x 150mm 3-in-1 electrode for pHi
series 2xx, 3xx, 6xx (with waterproof
connector)
A57198 12mm x 150mm 3-in-1 electrode for pHi
series 4xx and 5xx
pH Buffer Solutions
582517 pH 4.00 Buffer, Red, 6 x 475 ml bottle
582521 pH 7.00 Buffer, Green, 6 x 475 ml bottle
582525 pH 10.01 Buffer, Blue, 6 x 475 ml bottle
566575 pH assortment:
3 x 475 ml pH 7.00
2 x 475 ml pH 4.00
1 x 475 ml pH 10.01
3005 pH 4.00 Buffer, powdered, 12 pack
3006 pH 6.86 Buffer, powdered, 12 pack
3007 pH 7.00 Buffer, powdered, 12 pack
3008 pH 7.41 Buffer, powdered, 12 pack
3009 pH 9.18 Buffer, powdered, 12 pack
3019 pH 10.01 Buffer, powdered, 12 pack
3010 pH 12.45 Buffer, powdered, 12 pack
Refill Solutions
A51713 Calomel pHree refill solution, 4 x 118 ml
bottle
A60217 Ag/AgCI refill solution, 4 x 118 ml bottle
A58729 Low ionic strength refill solution,
4 x 118 ml bottle
511469 pHRESH refill solution, 1 x 120 ml bottle
NOTE
Please visit www.beckmancoulter.com for a
complete list of electrochemistry meters,
electrodes, and accessories.
NOTE
For MSDS information, please visit
www.beckmancoulter.com and click on
Customer Support, then MSDS.
NOTE
If you experience problems with your Beckman
Coulter electrode that these procedures cannot
resolve, call your local Beckman dealer. In the
U.S., please call the toll-free Product Support
line at (800) 742-2345.
Beckman Coulter Sealed and Refillable Electrodes
pH/Ref/ATC (3-in-1) Electrodes
Combination Ag/AgCI Electrodes
Combination Calomel pHree Electrodes
Combination Low Ionic Strength Electrodes
Combination pHRESH Research-Grade Electrodes
pH and Reference Electrodes
Beckman Coulter has a comprehensive offering of 3-in-1, combination, pH, reference, and specialty
electrodes in epoxy or glass bodies. Refillable or sealed models offer the same superior performance.
Helpful Operating Tips Figure 1. Removing the soaker bottle
1. Depending on the model, your new Beckman
Coulter electrode is shipped in either a soaker
storage bottle or a protective cap. Remove these
from the electrode, and retain for storage when
the electrode is not in use (See Figure 1).
2. If using refillable models, ensure fill solutions are
within 2.5 in. (6.4 cm) of the fill hole. Ensure fill
hole is open during use to prevent vacuums.
Sealed models require no special attention.
3. Combination electrodes combine pH and
reference cells in one electrode. Use of pH
electrodes require use of reference electrodes.
Attach appropriate connector to mating
connection on meter. If using a pH/Ref/ATC
(3-in-1) electrode, an additional connection is
provided for the temperature connection. If using
Refillable Models
a separate temperature sensor, attach it to the
appropriate mating connection. The fill holes should be open when measurements
are being made, when the junction is being renewed
4. Vigorously stir the electrode in the sample buffer
or the electrode is being refilled. It should be closed
or rinse solution. This action will bring the
at other times. The reference solution level should
solution to the surface of the electrode more
not be allowed to drop more than 2.5 in. (6.4 cm)
quickly and improve speed of response.
below the refill line in the electrode.
5. When calibrating, use a buffer close in value to
that expected from the sample. This will minimize Standard models use a sleeve to cover the fill hole.
span errors. Before use, slide the sleeve to open the fill hole and
allow air flow for measurement.
6. Keep buffers and samples at the same
temperature. This will eliminate the need to The pHRESH series electrodes feature Rapid-Renew
correct values for temperature effects. Reference Junctions. To open the sleeve-type
reference junction, press down on the spring-loaded
7. pH readings stabilize faster in some solutions than
Top Cap. While the junction is open, the solution
in other solutions. Allow time for reading to
inside the reference flows out of the electrode. This
stabilize. In general, buffers provide stable
flow flushes out contaminants. When the Top Cap is
readings within several seconds.
released, a new reference junction is formed. These
electrodes also feature a Twist-Seal design
reference solution fill hole. The hole is opened or
closed by rotating the knurled knob.
©2008 Beckman Coulter, Inc. 1
Printed in USA.
Calibration Procedure
As a rule, follow the procedures recommended by the
pH meter manufacturer, keeping in mind the Helpful
Operating Techniques in this guide. The
frequency of calibration is a function of the
electrode, the pH meter, and the solutions to which
the electrode is exposed. The electrode and meter
should always be calibrated together, with the
calibration frequency determined by experience. Use
the following procedure for both calibration in
buffers and for sample measurements (See Figure 2):
1. Remove the electrode from its soaker bottle or
cap. Save the bottle or cap for reuse.
2. Vigorously stir the electrode in a rinse solution.
3. Shake the electrode with a snap action to remove
residual drops of solution.
4. Vigorously stir the electrode in the buffer or
sample and allow the electrode to rest against the
wall of the beaker.
5. Allow reading to stabilize and take the reading.
6. Repeat these steps for each sample or buffer
determination.
Electrode Storage
When pH readings are made infrequently (several
days or weeks apart, for example), the electrode can
be stored by replacing it in its soaker bottle or cap.
First, slide the cap onto the electrode. Next, slide the
o-ring onto the electrode, insert the electrode into
the bottle, and tighten the cap. If storage solution is
not available, fill the bottle with pH 4 buffer.
Electrode Cleaning
Coating on the measuring surface can lead to
erroneous readings, including shortened span
(slope). Never use a brush or abrasive on any pH
electrode (see Figure 3). Instead, use the following
techniques to clean the electrode measurement bulb:
1. Soft coating can be removed by vigorous stirring
or by using a squirt bottle filled with water.
2. Organic, chemical, or hard coatings should be
removed by soaking the electrode in a 5-10%
hydrochloric acid (HCI) solution for a few
minutes.
3. Grease, oil, or other surfactants can be removed
by rinsing with a solution of 50% acetone and
50% isopropyl alcohol. Then rinse with deionized
water and soak in pH 4 buffer for one hour.
4. Protein can be removed by soaking the electrode
in a 10% bleach solution (9 parts deionized water
to 1 part bleach) for fifteen minutes. Rinse in
deionized water and soak in pH 4 buffer for fifteen
minutes. Rinse again in deionized water.
2 3
Figure 2. Calibrating the electrode Electrode Reconditioning
When reconditioning is required due to electrode aging, the following chemical treatments may be tried.
They are presented in the order of the severity of attack on the pH glass and may not improve, and in some
cases may actually deteriorate, electrode performance.
Figure 4. Reconditioning the electrode
WARNING
Use proper precautions when handling these
hazardous chemicals. Ammonium bifluoride
and hydrofluoric acid (HF) are extremely
hazardous and should only be used by qualified
personnel.
警告
请在处理这些有害化学物质时采取适当
的预防措施。氟化氢铵和氢氟酸 (HF)
极为有害，仅限合格的人员使用。
1. As shown in Step 1, immerse the electrode tip in
0.1 M HCI for fifteen seconds, rinse in deionized
water, then immerse tip in 0.1 M NaOH for fifteen
seconds. Rinse the electrode in deionized water
again. Repeat this sequence three times, then
recheck the performance of the electrode. If
performance has not been restored, proceed to
Step 2.
2. Immerse the tip of the electrode in a 20% solution
of ammonium bifluoride (NH4F•HF) for two to
three minutes, rinse in deionized water, and
recheck performance. If performance has not
been restored, proceed to Step 3.
3. Immerse electrode in 5% HF for ten to fifteen
Figure 3. Cleaning the electrode seconds, then rinse thoroughly in deionized water
(Step 3a). Rinse in 5N HCI, rinse thoroughly in
deionized water, and recheck performance
(Step 3b). If performance has not been restored, it
is time to replace your electrode.
WARNING
Always follow laboratory safety procedures,
including wearing protective gloves and eye
wear. Avoid consuming, inhaling, or coming
into direct contact with any chemicals or
solutions.
警告
始终按照实验室安全程序操作，包括佩
戴防护手套和眼罩。避免吞食、吸入或
直接接触化学物质或溶液