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Day 1 (afternoon): inoculate 50 ml culture SGI medium with yeast colony in 250 ml flask.
Grow at 28 C – 250 rpm
Day 2: -
Day 3 (afternoon): Centrifuge culture in bluecap 5 min 1000g (2300 rpm table centrifuge)
Resuspend pellet in 250 ml YPL medium
Grow at 28 C - 250 rpm o/n
Day 4 (morning): measure OD600 – YPL medium as blanc (about 6 is OK)
Microsome isolation