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 Test Your Knowledge

Discover whether your knowledge base is current and up to date! Answer the following questions and then check
the answer key below.

1. A patient with which of the following diseases 4. What gene is used for identifying CMV infection
would most likely be immunocompromised? by RT-PCR?
a. Chronic Obstructive Pulmonary Disease a. Glycoprotein B gene

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b. Tuberculosis b. Mitogen activated protein kinase
c. Chronic Myelogenous Leukemia c. Glycoprotein 120 gene
d. Crohn’s Disease d. Toll-like receptor 7

2. Which of the following is not a common symptom 5. Which of the following techniques is least helpful
identified in immunocompromised patients with in detecting the presence of a point mutation in the
Cytomegalovirus (CMV) infection? primer binding site?
a. Vanishing Bile Duct Syndrome a. Lowering the annealing temperature of the PCR
b. Graft rejection reaction
c. Aggressive bacterial and fungal infections b. Identification of changes in the melting temperature
d. Vasodilatation of the PCR product
c. Sequencing the PCR product
3. Which of the following tests is not used for clinical d. Immunohistochemical staining
detection of CMV infection?
a. Real-time polymerase chain reaction (RT-PCR)
b. Viral culture
c. pp65 antigenemia assay
d. DNA sequencing

If you’ve had difficulty with these Test Your Knowledge questions, please refer to the following article
for review: Cytomegalovirus-Antigenemia-Positive and Polymerase-Chain-Reaction-Negative
Transplant Patient (LabMed. 2008;39:341–342) by Amanda K. Hernandez, MS, Jacqueline
Emmons, MD, Daniel B. Wimmer, MS, Deborah A. Payne, PhD. These Test Your Knowledge
questions were generously submitted by CPT Anthony D. Kang, PhD, Medical Service Corps, US c, d, d, a, d
Army, Armed Forces Radiobiology Research Institute, Bethesda, MD. Correct answers:

248 LABMEDICINE ■ Volume 41 Number 4 ■ April 2010 labmedicine.com

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labmedicine.com April 2010 ■ Volume 41 Number 4 ■ LABMEDICINE 249

 Products
Polysciences
Test Tube Rack
Polysciences offers the Smooth Rack test tube rack, for holding test tubes firmly and
preventing them slipping out of position. The Smooth Rack holds a variety of tubes from
10 - 16 mm in diameter and has a 72 tube capacity. It is autoclavable, made of heavy-
duty polypropylene, and has excellent chemical resistance. The Smooth Rack is available
in either blue or white.
www.polysciences.com

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Polysciences
Digital Camera And Imaging Software
Polysciences offers the CMOS digital camera and imaging software. The camera
can preview live video and capture images without interrupting the live-video screen.
Captured images can be stored in over 10 different formats. Calibrated on-screen lin-
ear or area measurements can be done, including point to point, circle, angle, radius,
and free hand. Measurements can be superimposed and saved on the images. Image
processing allows interactive adjustment of hue, saturation, intensity, and sharpness;
in addition to noise suppression and removal of bad pixels. System requirements are
Windows XP, USB 2.0, Pentium III (or equivalent), 256 MB of system RAM, a video
card with either 24- or 32-bit true color graphics, and 8 MB of video memory.
www.polysciences.com

Polysciences
Staining Kit
Polysciences has the picrosirius red kit for staining fibrillar type collagen I and III. The kit
works with paraffin sections fixed for at least 24 hours in a neutral-buffered formaldehyde
solution. The kit has 3 bottles of solutions (A, B, and C). Specimens only require 2 min-
utes in solutions A and C, and exposure to solution B is 110 minutes. After staining, type
I fibrillar collagen is yellow, and type III fibrillar collagen is green.
www.polysciences.com

Phenix Research Products

Disposable Serological Pipettes
Phenix Research Products has the Phenix Series disposable serological pipettes. They
can be ordered either individually wrapped or as a bulk pack. They are a single piece
design and are available in 5, 10, and 25 mL sizes. The 5 and 10 mL sizes are also
available with a wide tip.
www.phenixresearch.com

Phenix Research Products

Freezer Rack
Phenix Research Products markets the Phenix Flexible Freezer Rack for 2- and
3-inch boxes. It is an upright freezer rack that can be modified with removable
clips to hold 2- and 3-inch boxes. The rack’s dimensions are 22 1/2 inches long,
9 5/16 inches high, and 5 1/2 inches wide.
www.phenixresearch.com

250 LABMEDICINE ■ Volume 41 Number 4 ■ April 2010 labmedicine.com

 Products

Phenix Research Products

Break-A-Way PCR Plate
Phenix Research Products offers the Break-A-Way PCR Plate. It has a 96-well plate
format and fits all cyclers. The plate has a standard profile, and it can be separated
without scissors into any combination of 8 walls. The plate can be sealed with either
strip caps or film.
www.phenixresearch.com

Phytotechnology Laboratories
Bottle Rack
Phytotechnology Laboratories has a bottle rack for both 250 mL (64 mm diameter)
and 500 mL (74 mm diameter) bottles. The rack holds up to 3 bottles of either size.
The rack also has a breakaway inner ring to fit 500 mL bottles.
www.phytotechlab.com

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Phytotechnology Laboratories
Sterilization Monitor
Phytotechnology Laboratories offers the Diack Sterilization Monitor to verify if a ster-
ilization temperature was reached during autoclaving. The Diack Sterlization Monitor
is a temperature-sensitive pellet, which melts at 121°C. The pellets can be stored at
room temperature.
www.phytotechlab.com

Phytotechnology Laboratories
Laboratory Dressing Forceps
Phytotechnology Laboratories markets Miltex Brand laboratory dressing forceps. They
are 9 1/2 inches long with 25 mm serrated tips. The forceps are German and hand made
of stainless steel.
www.phytotechlab.com

Fisher HealthCare
Coagulation Analyzer
Fisher HealthCare has the Roche Diagnostics CoaguChek XS System. It is a coagu-
lation analyzer, and it monitors patients under anticoagulant therapy. It tests for PT
levels and provides results in 1 minute. It stores up to 60 test results and does not
require calibration, pipetting, or reagents. The CoaguChek XS System includes a
monitor, battery, and a manual.
www.fishersci.com

Fisher HealthCare
Chemically Resistant Markers
Fisher HealthCare has the Fisherfinest chemically resistant markers. They leave
permanent marks on slides, cassettes, and most laboratory plastics. The markers
have fine tips and make smearproof and waterproof marks. The marks are resistant
to most chemicals, and the markers do not readily dry out. The markers are available
in black and come 12 per box.
www.fishersci.com

labmedicine.com April 2010 ■ Volume 41 Number 4 ■ LABMEDICINE 251

Products

Torbal Scales
Industrial Bench Balance
Torbal Scales has the BA Series Industrial Bench Balances. The balances have
die-cast metal bases and large stainless steel weighing platforms. Each unit also
has a USB port for easy PC connection. There is a large LCD display with backlight-
ing, a leveling system, an auto-tare function, and a tare storing function.
www.torbalscales.com

Torbal Scales
General Purpose Scale
Torbal Scales has the BT200D: a general purpose, low-priced scale for everyday
weighing. It is useful for low-capacity, high-resolution weighing. The BT200D has
rechargeable batteries, and it is portable. The scale has a draft shield to protect the
balance from air movement. Other features include a large LCD display with back-
lighting, an RS232 interface port, a leveling system, auto-tare, and tare storing.

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www.torbalscales.com

H & A Scientific
Laboratory Information Management System
H & A Scientific markets the IntelliLIMS, laboratory information management system
software, developed in accordance with FDA regulations. The software’s functional-
ity includes sample log-in and tracking, barcode support, scheduling, chain of cus-
tody, result entry with audit trails, and result reporting. IntelliLIMS allows access from
multiple computers, and it runs on Windows 2000, XP, Vista, and Windows Servers
2003/2008.
www.hascientific.com

Thermo Scientific
Reagent Dispenser
Thermo Scientific offers the Multidrop Combi nL, a nano to micro volume bulk
dispenser. The Multidrop Combi nL has precise and accurate submicroliter volume
dispensing. Also, high throughput is combined with full robot compatibility. Multidrop
Combi nL can be controlled with PC software, and an intuitive visual user interface
makes all fuctions easy to set up and use.
www.thermo.com

252 LABMEDICINE ■ Volume 41 Number 4 ■ April 2010 labmedicine.com

 ASCP Calendar
April 2010
1 th Audio Teleconference Molecular Diagnostics and Targeted Therapy for Colorectal Carcinoma
2 fr  udio Teleconference
A Standard of Care in Liver Biopsy Evaluation: What Is It?...And How Can It
   Be Used To Optimize Liver Biopsy Reporting?
6 tu Audio Teleconference Improving Patient Safety in Surgical Pathology
7 we Audio Teleconference A Passion for Challenge
8 th  udio Teleconference
A Diagnosing Thyroid Follicular Lesions on FNA Biopsy: How Helpful Are New
   Immunocytochemical Markers?

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8-14 th-we Resident Review Course (Hoffman Estates, IL)
12-14 mo-we Educational Course (Las Vegas, NV) Current Issues in Liver Pathology
13 tu Audio Teleconference Continued Importance of Blood Lactate Measurements in Critical Care
15-17 th-sa Educational Course (Chicago, IL) Update in Pulmonary Pathology: Contemporary Classification and Diagnosis
16 fr  udio Teleconference
A Well-Differentiated Lipomatous Tumors: A Common and Challenging
   Problem to the Practicing Pathologist
19-21 mo-we Educational Course (Ft Lauderdale, FL) Molecular Surgical Pathology for the Practicing Pathologist
20 tu Audio Teleconference Laboratory Testing In Hepatitis Syndromes: A Widening Spectrum
21 we Audio Teleconference Pathology: Error Reduction in an Error-Prone Environment
22 th Audio Teleconference Lung and Mediastinal Fine-Needle Aspiration Cytology - Newer Perspectives
27 tu Audio Teleconference Age-Specific Care for Phlebotomy
28 we Audio Teleconference Essential Components of a Successful Method Evaluation Experience
29 th Audio Teleconference Molecular Diagnostics of Thyroid Cancer
30 fr Audio Teleconference Liquid Gold : Review and Update of Routine Urinalysis

May 2010
4 tu  udio Teleconference
A Why Is the Diagnosis of Type 1 von Willebrand Disease So Difficult?
   Clinical and Laboratory Considerations
5 we Audio Teleconference Group B Streptococcus Update
6 th Audio Teleconference Endoscopic Ultrasound-Guided Fine-Needle Aspiration of Pancreatic Lesions
7-9 fr-su Weekend of Pathology (Toronto, Canada)
11 tu  udio Teleconference
A Non-RBC Components: Hemotherapy Decisions and Management of
  Transfusion Reactions
12 we Audio Teleconference Thromboelastography in the Clinical Laboratory
14 fr Audio Teleconference Legal Aspects of Phlebotomy
17-21 mo-fr Educational Course (Santa Fe, NM) Surgical Pathology of the Gastrointestinal Tract
19 we Audio Teleconference Update of Newborn Screening in the Public Health Laboratory
21 fr Audio Teleconference Cool Under Pressure: Survival Guide to Intraoperative Neuropathology

labmedicine.com April 2010 ■ Volume 41 Number 4 ■ LABMEDICINE 253

Calendar

26 we Audio Teleconference Using DNA-Based Testing To Manage Patient Care in Transfusion Medicine
27 th Audio Teleconference Liver Function Testing
28 fr Audio Teleconference Fixation Theory and Reagents

June 2010
1 tu Audio Teleconference
2 we Audio Teleconference
3 th Audio Teleconference
4 fr Audio Teleconference
8 tu Audio Teleconference
9 we Audio Teleconference
How Safe Is Your Laboratory From Occupationally Acquired Infections?
The Impact of NAT for HIV-1 and HCV on Blood Donor Reentry
Cervical Cancer Prevention in Vietnam: Lessons Learned
Chondroid Lesions of the Head and Neck
Low-Incidence Blood Group Antigens
Molecular Methods to Support Infection Control and Prevention Programs

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9-13 we-su Educational Course (Santa Barbara, CA) Dermatopathology: Contemporary Diagnositc Criteria and Strategies
10 th Audio Teleconference Introduction to Cytogenetics and Molecular Cytogenetics - Session 1
15 tu  udio Teleconference
A Laboratory Diagnosis of Infections Caused by the Dimorphic Fungi--Culture,
   PCR, and Serology
16 we Audio Teleconference The Gram Stain, a Real-Time Multiplex Classic Test in the Molecular Era
17 th Audio Teleconference Introduction to Cytogenetics and Molecular Cytogenetics - Session 2
18 fr Audio Teleconference A Practical Approach to Pulmonary Hemorrhage
23-25 we-fr Educational Course (New York, NY) Current Issues and Problems in Breast Pathology

July 2010
26-30
mo-fr Educational Course (Montreal, Quebec) Pathology Update: State-of-the-Art Diagnostic Approaches to
   Surgical Pathology

October 2010
27-31 we-su ASCP Annual Meeting (San Francisco, CA)

254 LABMEDICINE ■ Volume 41 Number 4 ■ April 2010 labmedicine.com

 Author Guidelines
LABMEDICINE LABMEDICINE is a monthly periodical that Preparing a First, determine the article type above that best
Mission and publishes original articles on scientific, technical, Manuscript meets the purpose(s) of the manuscript you
Scope managerial, and educational topics related to for Submission and your co-authors intend to submit to
laboratory medicine. Manuscripts are sent LABMEDICINE.
for peer review and published based on the
Then, find a recent article of the type you
quality of their content, reviewers’ comments,
selected, published previously in LABMEDI-
and their “audience appeal” to the readership CINE, and use the format of that article as
of LABMEDICINE. a template to format your manuscript with
considerable attention to the details of that format.
Types of The following types of manuscripts can be
Manuscripts submitted to LABMEDICINE. Important
note to authors: Prior to submitting

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your manuscript to LABMEDICINE using Section Contents
Manuscript Central (http://mc.manuscriptcentral.
com/labmed), please review the article types Title page Title of article; authors’ full names (first, MI, surname), degrees,
(especially their formats) published in any and/or nationally-recognized professional certification designations;
issue of LABMEDICINE and format your institution affiliation with complete mailing address, telephone and
manuscript to comply with the format require- fax numbers, and e-mail address of corresponding author.
ments of the article type most appropriate to Abstract • Write a brief (150 words) Abstract and use bullets to list key
the content of your manuscript. topics covered.
• Number pages consecutively, beginning with this Abstract.
Equipment Where applicable, list all equipment/instruments mentioned in the
article with the manufacturer’s complete contact information (eg,
company name, address, telephone number, and Web site address).
Manuscript Description
Type (Codes) Introduction Include the background information, with appropriate reference
citations to the references listed in the References portion of the
Solicited (S) An article solicited by the editors from a specific author(s). manuscript, related to the topic of the manuscript. Include a
Unsolicited (U) An article not solicited by the editors from the author(s). sentence(s) that inform the reader about the purpose of the work
reported in the manuscript.
Feature (F) An article solicited by the editors about a cover-story topic.
Materials and Describe with sufficient details to allow a reader to reproduce
Overview (O) A comprehensive overview of a particular scientific topic, also Methods your findings using all materials and methods used to obtain the
known as a Review article. findings presented in the manuscript.
Continuing Provides recent information on a selected topic and includes Results Describe the findings, preferably using tables, figures, graphs,
   Education 5 multiple choice questions. Similar to an Overview article, and/or images wherever pertinent. Ensure that legends
   (CE) Update only shorter and more concise. applicable to all tables, figures, graphs, and/or images included in
Case Study (CS) Reports on actual cases with an emphasis on educational value. your manuscript can “stand alone” (ie, all information necessary for
Case Study manuscripts must adhere stringently to the required the reader to interpret them is included in the legend such that the
   format (patient history/chief complaint/clinical and lab info, etc.; reader does not have to refer to any other section within the manu-
   “Questions,” including “most likely diagnosis”; and “Possible script for interpretive information relevant to tables, figures, graphs,
Answers” to all questions). and/or images).
Rounds (R) Uses a short Case Study format as a springboard to a mini-review Units of Use conventional (ie, non-SI) units.
of the clinical and laboratory aspects of the most likely diagnosis. Measure Note: LABMEDICINE does not use SI units.
   Typically, Rounds articles are similar to Case Studies but without Discussion Explain the meaning of the results, any significant implications, or
the “Questions” and “Possible Answers” portions of the Case additional studies required.
Study manuscript format.
Acknowledg- • Acknowledge any financial support.
Science (SCI) Original research article related to a laboratory medicine topic ments
likely to be of interest to laboratory professionals. Note: As • Acknowledge technical assistance or other collaboration involved
a general rule, LABMEDICINE does not accept manuscripts in the manuscript preparation. For example, indicate whether equip-
reporting basic research performed on animals. ment or procedures used were being field tested or if a diagnostic
company provided support (financial or otherwise) in the completion
Media Review A review of a book or another form of media. of the work.
   (MR)
References Authors bear full responsibility for the accuracy of references and
News Brief (NB) An item on a timely, news-oriented subject. other citations. References to unpublished works or verbal commu-
Letters (L) A letter to the editor concerning an article/topic published nications should appear within parentheses in the text and not in the
previously in LABMEDICINE. reference list at the end of the manuscript.
• Place the reference section at the end of the manuscript before
Q and A (QA) A question related to a laboratory medicine-related problem/topic the table(s) and image or figure legends.
that the author(s) wish to obtain an answer to from an appropri- • List references in the order in which they are cited in the manu-
ately-qualified and knowledgeable individual(s). script using standard reference styles (see below). Note: A single
Special Report A report on any timely (“hot-button”) topic related to any reference used more than once in the manuscript needs only one
   (SR) subspecialty of laboratory medicine. reference number.

labmedicine.com April 2010 ■ Volume 41 Number 4 ■ LABMEDICINE 255

Author Guidelines

Content and Length: Manuscripts should be between • the reviewers’ use of a structured reviewer’s
form that is subsequently sent to the “author
Sequence 1,200–2,400 words, including Abstract
for correspondence” for response;
and References.
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 get to
work!
ASCP Career Center

According to US News and World Report,

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your career as a medical laboratory scientist
or laboratory technician is one of the 50 Best
Careers in 2010.

opportunities at ASCP’s online career center.

Start your search today

at www.ascp.org/careercenter

Employers!
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 Congratulations to the winners
of the 2nd Annual ASCP Lab Professionals
Week Pizza Party!

Pinnacle Health Kosciusko Medical Clinic

Harrisburg, PA Kosciusko, MS

Sutter Roseville Medical Center Henry M. Jackson Foundation Naval

Roseville, CA Health Research Center
San Diego, CA

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Marcus Daly Memorial Hospital
Hamilton, MT Honorable Mentions

Our Lady of Fatima Hospital Fleming County Hospital

North Providence, RI Flemingsburg, KY

Internal Medicine of Northern Michigan Brazosport Regional Health System

Petoskey, MI Lake Jackson, TX

South Austin Medical Center Howard University Hospital

Austin, TX Washington, D.C.

Wright Medical Center Doctors’ Hospital of Michigan

Clarion, IA Pontiac, MI

National Medical Laboratory Professionals Week is fast approaching!

pproaching!
Are you ready? Find dozens of ideas to make your Lab Week
eek
celebration a success at www.ascp.org/labweek!

National Medical Laboratory

Professionals Week
is April 18-24, 2010.