Laboratory worksheet 3

Rocket Immunoelectrophoresis (RIEP)

NAME : Bilal Tahir
STUDENT ID: 0328555

Introduction [3 marks]

Aim [1 mark]
Results:

Part I: Data Collection & Interpretation

1. Enclosed your gel picture here & show how you obtain the rocket height value for each antigen.
[4 marks]
2. Record the values obtained in (1) here.

[1 mark]

SI. Unit Standard Concentration (mg/ml) Rocket height in mm

1 4.0 24

2 2.0 16

3 1.0 7

4 0.5 3

5 Test 1 22

6 Test 2 10
3. Based on your data in (2), plot a standard curve. [3 marks]

Title:
Part II: Discussion Questions

1. From the standard curve, determine the concentration of the test antigen. [1 mark]

2. Discuss the principle of rocket immunoelectrophoresis in 3 to 4 sentences. [4 marks]

3. Rocket immunoelectrophoresis is an adaptation of the radial immunodiffusion (Prac 1). Discuss

the advantage and disadvantage/limitation of rocket immunoelectrophoresis as compared to radial
immunodiffusion. [4 marks]
4. Give one example of clinical (or industrial) application of rocket immunoelectrophoresis. Discuss
in details how this assay is set up. [5 marks]

Conclusion [2 marks]

Marks: / 28