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I. GENERAL OBJECTIVE
After completing this the students will be able to perform Female Venereology
Examination
III. METHODE
Presentation
Coaching
Self practice
IV. EQUIPMENT
Audiovisual aids
Learning guide
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V. COURSE STUDY GUIDE HISTORY TAKING AND VENEREOLOGICAL EXAMINATION
PERFORMANCE
No. PROCEDURE SCALE
0 1 2
CLIENT ASSESSMENT
01. The patient should be given adequate explanation about the
examination (purpose, procedure)
02. Ask the patient to urinate before examination.
03. Ask permission before the examination be carried out.
Ask the patient to take off her under wear, then lay down in
lithotomy position on the examining table
04. Wash your hands and dry with clean dry towel, then put on the
gloves.
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position of the speculum by tightening the thumb screw.
12. Inspect the vaginal wall for the presence of erythema,
discharge or other lesions such as: papules, vesicles,
erosions, ulcers. If discharge is presence, note the amount,
color and consistency.
13. The external ostium of the cervix is wiped clean to remove
mucus using forcep with gauze or cotton wool.
14. Inspect the exocervix for the presence of erythema, ectopy,
discharge, and lesions such as papules, vesicles, erosions,
ulcers. If discharge is presence, note the amount, color and
consistency.
SPECIMEN COLLECTION : examiner in sitting position
15. Neisseria gonorrhoeae :
Insert a cotton-tipped swab about 2 cm into the cervical
canal. Rotate the swab gently for 5 – 10 second to allow
absorption of the exudates. Smear thinly the specimen
on a microscopic slide for the Gram staining (look for
PMN cell and Gram negative intra celular diplococcus
(ICDC))
Second swab is done with the same way and placed into
transport medium for culture.
16. Chlamydia trachomatis :
Insert a chlamidya specimen collecting swab 2 cm into the
cervical canal and rotate it for 10 second, gently scrap epithelial
cells from the surface. After that, place the specimen into the
transport medium for antigen/nucleic acid detection.
17 Clue cell:
Insert other swab into introitus vagina. Swab the vaginal wall,
put it to the microscopic slide for Gram staining, and look for the
Clue cells. (Microscopic interpretation: Positive if clue cell >
20% of epithelials per high power field)
18 Candida sp.:
Prepare a microscopic slide first which already been add
drop of 10% potassium hydroxide.
Insert other cotton-tipped swab to vaginal wall, and put it
on the slide and cover with cover slip. (Microscopic
intepretation: Look for pseudohyphae, budding cell)
19 Trichomonas vaginalis:
Prepare a microscopic slide first which already been add
drop of saline.
Insert other cotton-tipped swab to the posterior vaginal fornix,
and put it on the slide and cover with cover slip (Microscopic
intepretation: Look for the motion of Trichomonas vaginalis)
pH DETERMINATION OF VAGINAL FLUID
20. Hold the pH paper with forcep, then apply it to the vaginal wall
near the lateral fornix for a while. Pull it out and measure the
acidity of vaginal fluid by comparison with a standardized color
scale.
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WHIFF TEST/AMINE TEST
21. Remove the speculum gently.
Add a drop of 10% potassium hydroxide to vaginal fluid
pooling at the speculum.
The test is positive if smell of “fishy odour”.
Pull the speculum and put it in the basin filled with
decontamination solution.
CLOSING
24. Tell to the patient that the examination is finish and ask her to
get dressed. All of the examination result is written to the
medical record and fill the laboratory form.