Post-translational Modification Analysis

Post-translational modification (PTM) refers to the covalent, usually

enzymatic modification of proteins, and protein process during or after
protein biosynthesis. Protein post-translational modification (PTM) increases
the functional diversity of the proteome by the modifying proteins with
functional groups, such as phosphate, acetate, amide groups, or methyl
groups, and influences almost all the aspects of normal cell biology and
pathogenesis. It plays a key role in many cellular processes such as cellular
differentiation, protein degradation, signaling and regulatory processes,
regulation of gene expression, and protein-protein interactions. The
modifications genererally include phosphorylation, glycosylation,
ubiquitination, nitrosylation, methylation, acetylation, lipidation and
proteolysis and influence almost all aspects of normal cell biology and
pathogenesis. Therefore, characterization of PTM, including the modification
categories and modified sites, is critical in the study of cell biology and
disease diagnostics and prevention.

Identification of Post-Translational Modifications (PTM) is a tedious process.

It can be affected by many factors. For example, most of the
post-translational modifications are present in very low level. Therefore,
enrichment steps are necessary before identification process. Additionally,
stability of modification, and detection efficiency of mass spectrometry are
also critical factors in PTM identification process. Creative Proteomics offers
an advanced analytical platform for the characterization of various
post-translational modifications (PTM).

overview

Function of PTMs:

Category of PTMs Functions

Phosphorylation Change protein comformation; Activate/inactivate catalytic
activity
Ubiquitination Target protein for degradation
Glycosylation Direct a protein to its destination
Acetylation Affect protein comformation and its affinity to other proteins
Lipidation Affect the activity and subcellular location of a protein
Methylation Affect protein comformation and its affinity to other proteins
Proteolysis Remove peptide sequences or regulatory domains

Types of PTMs analysis service we provide:

Phosphorylation
Glycosylation
Ubiquitination
S-Nitrosylation
Biotinylation
Methylation
N-Acetylation
Lipidation
S-myristoylation
S-prenylation
Alkylation
Glutamylation
Workflow of our PTMs analysis service:

Digestion of proteins into small fragments

Protein separation and analysis using LC/MS/MS
Database search
PTM mapping
Full protein annotation
Technology platform:

2D Fluorescence Difference Gel Electrophoresis (DIGE)

Liquid Chromatography (LC)
High Performance Liquid Chromatography (HPLC)
Matrix Assisted Laser Desorption Ionization Mass Spectrometry (MALDI-MS)
Ordering Procedure:

Ordering Procedure

Our customer service representatives are available 24 hours a day, from

Monday to Sunday.