Protein Methylation Analysis

Protein methylation is a post-translational modification (PTM) process, in

which highly specific enzymes called methyltransferases are responsible for
the addition of methyl groups to a targeted molecule and S-adenosyl
methionine (SAM) as the primary donor of methyl group. Protein methylation
commonly occurs on arginine, lysine, histidine, proline, and carboxyl groups.
Protein methylation plays an important role in modulating cellular and
biological processes, including transcriptional regulation, RNA processing,
metabolism and signal transduction.

protein-methylation-analysis-1.jpg

Figure 1. Types of methylation on arginine residues (Yang Y and Bedford M T,

2013).

Overview of Protein Methylation Service

Creative Proteomics has already developed a highly sensitive HPLC-MS/MS

pipeline that can analyze N- and O-methylation. With powerful and sensitive
tools, we can identify, quantify and characterize protein methylation. Metabolic
labeling strategies can be coupled with MS to measure dynamic and
differential in vivo protein methylation rates. In addition, we also provide
bioinformatics services in Protein Post-translational Modification Analysis. We
have optimized our protocol to enable more fast and sensitive services for
methylation analysis. Shown as Figure 2, our protein methylation service
contains digestion, enrichment, LC-MS/MS analysis, and data analysis.

protein-methylation-analysis-2.jpg

Figure 2. The workflow of protein methylation analysis.

Sample Requirements

Plant roots, xylem, phloem, etc.: 5g or more

Animal tissues: wet weight >200 mg
Microorganisms: wet weight > 2 g
Body fluids (saliva, amniotic fluid, cerebrospinal fluid, etc.): 10 mL or more
Serum: 500 μL or more
Urine: 50 mL or more
Protein extract: concentration > 2 mg/mL, total not less than 1 mg. In order to
ensure the test results, please inform the buffer components, whether it
contains thiourea, SDS, or strong ion salts. In addition, the sample should not
contain components such as nucleic acids, lipids, and polysaccharides, which
will affect the separation effect.
If you want to know specific samples requirements, please feel free to contact
us.

Delivery

Detailed report, including experimental materials, methods, procedures, and

results
Raw data and data analysis result
Our Advantages

High-throughput: Identify and quantify up to thousands of proteins at once

100% coverage: Use 2-3 enzymes for protein digestion to ensure full-coverage
Ability to analyze low abundance ubiquitin-proteins
State-of-the-art facilities: Triple TOF 5600, Q-Exactive, Orbitrap Fusion Tribrid
Constantly optimized and validated protocol
Technology platforms

Ion Chromatography
High Performance Liquid Chromatography (HPLC)
Matrix Assisted Laser Desorption Ionization Mass Spectrometry (MALDI-MS)
Matrix Assisted Laser Desorption Ionization Mass Spectrometry (MALDI-MS)

At Creative Proteomics, many excellent and experienced experts will optimize

the experimental protocol according to your requirement and guarantee the
high-quality results for protein methylation analysis. As every project has
different requirements, please contact our specialists to discuss your specific
needs.

Reference

1. Yang Y, Bedford M T. Protein arginine methyltransferases and cancer.

Nature Reviews Cancer, 2013, 13(1): 37.

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Monday to Sunday.