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Article history: Objective: Berberis aristata (Berberidaceae) is an important medicinal plant, found in the
Received 15 June 2012 different region of the world. It has significant medicinal value in the traditional Indian and
Received in revised form 27 July 2012 Chinese system of medicine. The aim of the present investigation includes qualitative and
Accepted 18 October 2012
quantitative analysis of Berberis aristata extract. Methods: Present study includes determination
Available online 28 October 2012
of phytochemical analysis, solubility test, heavy metal analysis, antimicrobial study and
quantitative analysis by HPTLC method. Results: Preliminary phytochemical analysis showed the
Keywords: presence of carbohydrate, glycoside, alkaloid, protein, amino acid, saponin, tannin and flavonoid.
Berberine Solubility in water and alcohal were found to be 81.90% in water and 84.52% in 50% in alcohal.
Berberis aristata Loss on drying was found to be 5.32%. Total phenol and flavonoid content were found to be 0.11%
HPTLC and 2.8%. Level of lead, arsenic, mercury and cadmium complies the standard level. E. coli and
Phytochemical analysis salmonella was found to be absent whereas total bacterial count, yeast and moulds contents
Standardization were found to be under the limit. Content of berberine was found to be 13.47% through HPTLC
techniques. Conclusions: The results obtained from the present studies could be used as source
of valuable information which can play an important role for the food scientists, researchers and
even the consumers for its standards.
Organoleptic study revealed that the extract has brown V4.06 S/N:0612A004 CAMAG SOFTWARE (c) 1998 SCANNER 3: 061121
Track 1, Analysia a: be5mcl
colour and bitter test. Preliminary phytochemical analysis Peak Start max end area
of Berberis aristata showed the presence of carbohydrate, # Rf H Rf H [%] Rf H F [%]
glycoside, alkaloid, protein, amino acid, saponin, tannin 1 0.27 0.2 0.25 51.8 11.82 0.41 12.4 2120.8 7.73
and flavonoid. TLC analysis incorporating solvent system 2 0.41 12.4 0.50 386.8 88.18 0.62 3.0 25325.9 92.27
Total helght=438.7 total area=27446.7
(Benzene: ethyl acetate: diethyl amine (6: 3: 1)) showed to
contain three prominent spots with Rf (0.02, 0.05, 0.01) and in Figure 1(A): HPTLC chromatogram of standard berberine marker
(n-propanol: formic acid: H2O (90:1:9)) showed to contain four compound.
prominent spots with Rf (0.12, 0.07, 0.35, 0.07) respectively.
Parameter such as solubility in water and alcohal were
S138 Dinesh K. Patel et al./Asian Pacific Journal of Tropical Disease (2012)S136-S140
70000
y=613.9x+1059.1
60000 R =0.996
2
5
500
4
50000
400 40000
Peak area
30000
300
1
20000
200 3 10000
0
100 2 0 20 40 60 80 100 120
Concentration
0
0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1.0 Figure 2: Standard calibration curve of berberine.
[Rf]
Wavelength: 254 nm
Track: 2, noise level: 0.473AV, raw data file: DINBER_2
V4.06 S/N:0612A004 CAMAG SOFTWARE (c) 1998 SCANNER 3: 061121
Track 2, Analysia a: ba5mcl
Peak Start max end area
# Rf H Rf H [%] Rf H F[%]
1 -0.01 5.5 0.05 186.2 16.48 0.11 11.0 6019.8 7.03
2 0.11 11.0 0.11 12.6 1.11 0.14 0.1 142.7 0.17
3 0.25 0.0 0.35 103.9 9.20 0.40 54.1 5292.0 6.18
4 0.40 54.1 0.49 396.8 35.04 0.63 76.1 34138.3 32.88
5 0.64 76.9 0.87 431.1 38.16 1.04 2.4 40043.3 4676
Total helght=1129.5 total area=85636.1
500
450
400
350 In UV 254 nm
300
250 Figure 3(A): HPTLC photograph of standard berberine and Berberis
200 aristata extract at 254 nm.
150
100
50
0
0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1.0
[Rf]
Wavelength: 254 nm
File name: DINBER_2 track 1 to 3
V4.06 S/N:0612A004 CAMAG SOFTWARE (c) 1998 SCANNER 3: 061121