Documente Academic
Documente Profesional
Documente Cultură
MARCH 2019
CHAPTER 1
INTRODUCTION
Bacteria can spread throughout our body and cause some infections that
willaffect into some infections. Doctors, scientist and researchers is making a move to
prevent such problem. We can’t predict where bacteria occurred or why get some
infections specifically, skin diseases. Peoples health are the important key of this
Asiatica to be the main ingriedients of the soap. In many studies it is found out that C.
asiatica has antimicrobial activity and can be good anti-bacterial soap. According to a
study of Shinomal and Muralidhara (2011), Gotu kola were suggested as a treatment
of dermatoses skin lesions such as, burns hypertrophic scars, eczema, excoriations.
Moreover, C. asiatica contains triterpenoids that can strengthen the skin and bring
vitamins such as Vitamin C, A B1,B2, niacin and beta carotene that is known for skin
brightening.
Gotu kola were used in many cosmetics products such as creams, facial wash
and etc. the role of the soap is to help lessen the problem of expeditious spreading of
the bacteria. Many researches proven the anti-bacterial properties of the plant
(Centella Asiatica) extract and usinf that extract as on eof the ingredient for producing
soap.
staphylococcus bacteria?
2. Is the soap making using the gotu kola extract feasible?
3. Does soap making using Guto kola extract as main-ingredient cost-
efficient?
CONCEPTUAL FRAMEWORK
INDEPENDENT VARIABLE
DEPENDENT VARIABLE
IMPLICATION
HYPOTHESIS
1.The production of soap incorporated with Gotu kola extract as main-ingredient is
feasible.
2.The gotu kola extract exhibits antibacterial activity towards staphylococcus aureus.
3.Soap making using Gotu kola extract as a main ingredient is cost efficient.
feasibility of Gotu kola based soap will generate data that will attempt to help people
who are seeking with cost efficient and environment fiendly soap. Likewise, the data
obtained could serve as the baseline information for future researchers that will
undergo with similar study. Furthermore, this will help also entrepreneurs who are
This study will deal with Gotu Kola (Centella Asiatica) extract, its
antimicrobial activity towards staphylococcus aureus and as the main ingredient for
the soap. Therefore, this study will evaluate the effectiveness of the extract by
measuring tge extent of inhibition towards staphylococcus aureus bacteria. Also this
production of the soap will be limited to bath soap using cold process.
DEFINITION OF TERMS
CENTELLA ASIATICA – Commonly known as Gotu Kola as main
study.
native to land of tropical regions of both hemispheres including India, and extend all
over to Sri Lanka, parts of China, Western South Sea Islands, Mexico South East
USA, South Africa, Columbia, Eastern South America, Venezuela and Madagascar
(Zahara etal., 2014).Due to its therapeutic use it is feasible to use use th whole plant
including leaves, stem and roots.( Brinkhaus et al., 2000). C. asiatica are found in the
damp places and wet places such as paddy fields of river banks that can maximixe its
growth. However, the Gotu Kola habitation are in sandy loams rather than clayey
triterpenenes are the bioactive compound found in the plant such as the
wounds and raise blood flow in the wound and the surrounding area and built up the
skin elasticity.
2.2 ANTIMICROBIAL
through viable cell counts method, find out that the alcohol extract of its plant shows
the bactericidal action within 2 hours against the Staphylococcus aureus, Shigella
species and Vibrio cholera. Moreover, it is believed that it C. Asiatica has active
microorganisms ( Talabani et al., 2016). Further, Talabani et al. (2016) reported that
Gotu kola leaves where rich in asiaaticoside and Asiatic acid as a good primitive
Even it is not found the exact scientific basis according to Ullah et al., 2009) it has
been shown that the leafs extract can be credible as an antibacterial agents of
Escherichia coli. In the study of Seeraratnam et al., 2012 states that C. asiatica is a
fatty acids and is prepared through hydrolyzing a fat alkaline (basic) conditions
an acid and a base to form a salt which an olive oil, coconut oil or vegetable oil can be
your acid. An acid is a molecule or ion that contributes protons or hydrogen ions (H+)
and/or accepts electrons, and a base is any substance that donates electrons or
hydroxide ions (OH- ) and/or that accepts protons.( Domingues & Schul, 2014)
Further the two procedures for soap making are given “cold” and “hot”. Also in the
study of Debesh Mishra soaps were created by saponification process which is, the
soap has the reacting oil containing the triglycerides with caustic soda or sodium
Study found by Zahara et al. , 2014, found out the vitamins A,(retinol),
(niacin) and carotene that is good for skin brightening. Vitamin C (ascorbic acid)
discovered as acting role of repairing damaged skin and the stimulator of collagen
production (Boyera et al., 1998; Barneset et al., 1975) and also in the study of
Hashim, 2015 Vitamin C also used as a ppositive control and a probable treatment of
cells. Likewise, in the study of Lee et al., (1992) Vitamin A shown the endothelial
effect proliferation when there is wound healing process. Gohil et al. C. asiatica could
be a good model for reducing skin aging by lowering capillary filtration and meant to
appearance of skin aging , collagen and topical scars due to its dose (Hamidpour et al.,
2015).In addition, it is recognize that the plant extract has the ability to stimulate
wound healing and used in pharmaceutical and skin care applications (Loiseau and
Mercier, 2000). Conferring to the study of Hashim et al., (2011) have reveled the C.
lotions, creams and etc. ( Zahara et al., 2014). In accordance to the study of Hashim
(2014) it is introduce as an skin care products that has been claimed as the one who
reconstruct collagen formation and to build up the damaged tissues and leads into
restoring the skins elasticity and firmness as well the improvement of its skin
feasible wound healing properties.( Hong et al., 2005; Shetty et al.,2006). In detail of
C.asiatica has the new topical preperations for various indications: nourishing skin
METHODOLOGY
RESERCH DESIGN
This study will used Experimental design. This study will test the Anti-
bacterial efficacy of a soap towards the specific bacteria Staphylococcus aureus. This
After the process of extraction of Gotu kola its extract will undergo the testing
bacteria. Its extract will be added also to the mixture of the soap using it as the main
ingredient of the soap containing its extract depending to its designed dosage. The
experiment will have to make three soap with different amount of extract every soap.
extract added to the soap. These three soaps also undergo the antibacterial test to see
aureus bacteria.
The materials which the researchers will use are Gotu Kola plant and the
NaOH solution, also the oils consisting the olive oil, palm oil and coconut oil and
distilled water. The soap bacteria testing will staphylococcus aureus bacteria.
The equipment are beaker, stirring rod, pot (casserole), analytical weighing
scale, plastics soap molders. Furthermore, this research will use laboratory
experimental method for the evaluation of the Gotu kola extract against
consist of different stages, the first is the preparation of all raw materials, next is the
First step of this research is to prepare NaOH (sodium hydroxide) with 74.35g
and distilled water with 111.52g to create a 40% solution of lye, then prepare the raw
materials in making the soap including the Gotu kola plant, and oil total of 500g such
as olive oil with 275g, palm oil with100g and coconut oil with 125 g.
fresh 100g Gotu Kola will be chopped and be placed inside the blender. The mixture
will then be added with distilled H2O with 200g. Thereafter, the resulting mixture
will be blended for about 2-3 minutes. This will then be filtered using filter or
cheesecloth.