BIOTECHNOLOGY 8

Pre Test
Name: _____________________________________ Year & Section: _______________ Date: ______________
I. Multiple Choice: Choose the best answer, write only the letter of the correct answer on your answer sheet.
_____1. Which of the following is an example of a product easily found in nature?
a. Antibiotic b. GMO c. Recombinant DNA d. All of the above
_____2. In a biotechnology lab, tap water is best used for___________________.
a. Making buffer c. the final rinse when washing glassware
b. b. washing glassware d. all of the above
_____3. The order of amino acids on a polypeptide chain is encoded by______
a. DNA b. mRNA c. rRNA d. Regulatory protein
_____4. How does DNA molecules vary close from one species to another?
a. The type of nitrogenous bases they contain c. the way nitrogenous bases pair
b. Number of genes and non-coding regions d. The directionality of the strand
_____5. What is Agar?
a. Solid media on which bacteria grow c. Region on bacterial operon
b. Liquid media for bacterial growth d. Matrix used for vertical get electrophoresis
_____6. What part of an amino acid differs from one to the other?
a. Amino acid b. Carboxyl group c. R group d. All of the above
_____7. Which of the following is a codon?
a. ATA b. UAU c. Tyrosine d. All of the above
_____8. What are the most common molecules in cells?
a. Sugars b. Lipids c. Proteins d. Nucleic Acids
_____9. Glucose is a _______ a. Peptide b. polysaccharide c. disaccharide d. monosaccharide
_____10. Where does translation occur in prokaryotes?
a. Endoplasmic reticulum b. Ribosomes c. Outside the cell d. Nucleus

II. Please match the word with the description.

Activity assays Genetic Engineering Recombinant DNA
Concentration assays PK/PD assays Bacteria cells
Molarity Transcription Potency assays
Buffer Plasmids Gene therapy
pH Spectrophotometry Genetic disorders
Biotechnology HPLC Gel electrophoresis
Translation Acid Choromatography
ELISA Assay

__________11. A process of making an RNA code from DNA.

__________12. A process in which RNA is decoded and polypeptides are formed.
__________13. Extra small rings present in some bacteria.
__________14. Modifications of DNA code of an organism.
__________15. Parkinson’s diabetes, cystic fibrosis (CF) are examples of ____.
__________16. Do not contain nucleus or membrane bound organelle, So, DNA is floating in cytoplasm
__________17. Helps researchers measure the amount of protein present.
__________18. Determines the amount and length of activity for a given protein in a test organism.
__________19. Shows if a specific protein is present and conducting the expected reaction.
__________20. A highly specific test to determine protein concentration based on a particular antigen-antibody interaction
__________21. Used for understanding the relationship between dosage and activity of a given pharmaceutical.
__________22. A test for determining the presence or a particular characteristic of a protein of interest.
__________23. An aqueous solution that ionizes and yields hydrogen ion.
__________24. A solution that resists changes in pH.
__________25. A measurement of the concentrations of hydrogen ion in a sample
__________26. Separation of molecules on or through a stationary phase.
 __________27. Quantitative study of electromagnetic spectra deals with visible light, near ultraviolet, and near-infrared light sources.
__________28. Form of liquid chromatography used to separate compounds that are dissolved in solution. Instruments consist
of a reservoir of mobile phase, a pump, an injector, a separation column, and a detector.
___________29. Defined as the moles of solute per liter of solution.
___________30. Using electricity to separate molecules on a gel slab.
___________31. Process of correcting DNA codes that cause genetic diseases and disorders.
___________32. The study and manipulation of living things or their components.
___________33. A combination of DNA from different sources in one molecule.

III. Answer the following questions.

34. A piece of one strand of a DNA molecule has the following code on it ATG CCC GTG TTA AAA TGT GGG ATC CCC GGT
GTG CCC TTA. What is the nitrogen base code opposite DNA strand?

35. A piece of structural gene has the code TAC CCC ATG GGG TAA GGC GTC. What is the mRNA transcript made?

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