Documente Academic
Documente Profesional
Documente Cultură
Cardio
Mycoplasma Pneumonia
- Atypical (walking Pneumonia)
- <40 age
- Short rod that doesn’t have a rigid cell wall (so it will not pick up gram stain)
- B lactamase resistant
- P-1 adhesin
- Damages the base of cilia (pertussis immobilizes cilia with mucous)
- Can make H2O2 to damage respiratory epithelium
- Diagnosed more readily with Serology
- Treat with a Macrolide, tetracycline or Fluoroquinolone (not tested in micro course)
Chlamydophila Pneumonia
- Atypical (walking Pneumonia)
- Very similar to Mycoplasma
- Obligate intracellular
- Presents with Inclusion bodies
- Elementary body is the infectious body but it is NOT metabolically active
- Reticulate body Metabolically active after invasion NOT infectious
Legionella pneumophilia
- Gram -ve rod
- Can cause Legionaries disease or Pontiac fever
- Most common infection is with L. pneumophilia serogroup 1
- Cultured on Buffered charcoal yeast extract (BCYE)
- Symptoms will include GI and CNS problems along with Hematuria
- Comes from a water source such as aerosols or sprinklers
- Live in alveolar cells and inhibit phagolysosome formation
- Urinary antigen test will be looking for the serogroup 1 (if negative and legionarres is
still suspected a lung culture will need to be done)
- Can do Direct Fluorescent Antibody stains, PCR and serology
Pseudomonas Aeruginosa
- Found in fresh water
- Can cause Otitis externa (swimmers ear)
- Risk for acquiring this bacterium is higher when you have Cystic Fibrosis and burn
wounds (CF also makes it easier Burkholderia Cepacia to infect the person)
- Strictly aerobic and does B and Y hemolysis with mucoid colonies
- Makes a green pigment called pyocyanin and Fluorescein in agars and sputum
Mycobacterium Tuberculosis
- Cough that lasts more than 6 weeks
- Aerobic
- Takes a very long time to grow (2-8 weeks)
- M. Bovis can be used to make a vaccine for TB
- Mycolic acid on the cell wall can be destroyed by heat
- Immunocompromised and patients with AIDS (low CD4 count) are more susceptible to
TB infection
- CD4 with the alpha interferon protect against TB
- Survives intracellularly in alveolar macrophages (inhibits phagolysosome like
Legionella) Mycolic acid have sulfolipids that help with this process
- Cell wall lipids, Lipoarabinomannan and superoxide dismutase helped resist lysosomal
enzymes and ROS
- Also have siderophores: Exochelins
- Requires Lownstein Jensen agar and Oleic acid-albumin broth for culturing
- For microscopy, it needs Ziehl-Neelsen stain and Rhodamine Auramine fluorescent stain
Chlamydophilia Psittaci
- Causes Ornithosis or Parrot fever
- Obligate intracellular bacteria which enters through respiratory system
- Can be transferred from any bird through inhalation of shit and urine
- Has the Elementary body (virulent) and Reticulate body (reproductive) forms
- No Peptidoglycan despite being a bacterium but uses Outer Membrane Proteins which is
found on all Chlamydia
- LPS for endotoxicity
- Affects the Reticuloendothelial cells of the body
- Causes Edema, infiltration of macrophages and necrosis. Mucous in Bronchioles can
cause Cyanosis.
- Main symptoms to look for are CNS and GI with hepato and splenomegaly and Follicular
keratoconjunctivitis
Burkholderia Pseudomallei ( Melioidosis)
- Gram -ve bacteria, facultatively intracellular
- Found in SOIL and fresh water and enters through SKIN (can enter through respiration
too)
- Endemic to Asia and north Australia
- High risk individuals have: CF (like P.Auriginosa), Alcoholism, Renal disease, Lung
disease, Thalassemia
- Use Ashdowns Agar to culture B. Pseudomallei
- Bipolar staining on Microscopy
Histoplasmosa Capsulatum
- Endemic to the Mississippi river valley and the Ohio river Valley
- 2 types H. Capsulatum Var capsulatum (Pulmonary and disseminated infections) and Var
Duboisii ( Skin and bone lesions )
- Var capsulatum is found in east US and Latin America and affects thinner cell walls. Var
Duboisii is found in Africa and affects large cell walls
- Found in soil with high nitrogen content and Bird or bat droppings
- Can affect immunocompetent or immunocompromised patients
- Presents with Hilar lymphadenopathy (like TB )
- Grows inside macrophages and increases pH of phagolysosome
- Microscopically: non-encapsulated thick walled budding yeast within macrophages
Blastomyces Dermatitis
- Endemic to the Mississippi river valley and the Ohio river Valley
-Can affect immunocompetent or immunocompromised patients
- Granulomatous Lesions on the skin or mucous membranes
- Very similar to histoplasmosis
- Microscopically it will have a single broad based bud in a yeast
Cryptococcus Neoformans
- Affects immunocompromised individuals
- Encapsulated
- Transmission through inhalation of the yeast. When inhaled it will produce a capsule
made of GMX (Glucuronoxylomannan). Capsule can be found in blood and body fluids
which downregulate immune system even further
- Oxidizes catecholamines to Melanin to prevent oxidative damage.
- Can cause sarcoidosis and liver disease
Pneumocystis Jirovecci
- Affects immunocompromised individuals
- Usually affects patients with a CD4 count of < 200 cells
- Lacks ergosterol in the cell wall
Aspergillus
- Found in decaying matter, air and soil
- Found in a septal hyphae form at an acute angle
- Has 2 forms: Allergic- asthmatic and cystic fibrosis and an Invasive form: hyphae
invaded tissue.
- “Fungal balls” can form – Aspergilloma
- Can cause acute pneumonia
- HEMOPTYSIS is a common symptom
Gastrointestinal
Remember that Aerobic bacteria are found closer to mouth and Anaerobic closer to the stomach.
Ecoli and streptococci obtained from mother upon birth.
Bifidobacterium kept in baby if it is fed breast milk ( needs this microbe to break down breast
milk)
Lactobacilli are needed to break down the sugars in formula
Eruption of your first teeth you will have Porphyromonas and prevotella and fusobacterium but
growth of teeth gives strep. Sanguis mutans and salvarius which give you dental plaque
Oesophagus has : Streptoccous, Prevotella and Veillonella
Stomach has: H. Pylori
Duodenum: Streptocci, Staphylocci, Lactobacilli, yeast
Jejunum-Ileum: Strep and staph lactobacillus, Bacteroides, Bifidobacterium and clostridium
Large intestine: BACTERIODES AND CLOSTRIDIUM. As well as Bifidobacterium,
Eubacterium, peptostreptoccocus and enterobacteriacea
Increased bacteriodes and decreased enterococci in meat eaters- opposite for vegetarians