Documente Academic
Documente Profesional
Documente Cultură
Isolation and Identification of High Cellulolytic Activity of Bacteria from Golden Snail’s (Pomacea canaliculata) gut
Isolasi dan Identifikasi Bakteri Selulolitik dengan Aktivitas Tinggi dalam Saluran Pencernaan Keong Emas (Pomacea
canaliculata)
Author :
M. Anam Al-Arif |
Fakultas Kedokteran Hewan Universitas Airlangga
Win Darmanto |
Fakultas Sain dan Teknologi Universitas Airlangga
Ni Nyoman Tri Puspaningsih |
Fakultas Sain dan Teknologi Universitas Airlangga
Suwarno |
Fakultas Kedokteran Hewan Universitas Airlangga
Abstract
Cellulase can degrade cellulose to become glucose. A large number of cellulolytic microbes are available in the
animal’s gut. Theaim of this research was to isolate and identify the cellulolytic bacteria with high activity from
Golden snail’s gut. Some golden snailswere surface sterilized by 70% ethanol and then washed in sterile distilled
water. The entire guts were removed from the abdomenand disrupted then 1 gram of gut debris was suspended in 10 ml
sterile distilled water. After serial dilution, aliquots were transferredinto CMC-solid media and incubated at 35° C for 24
hours. The growing cultures were cultivated on NA-solid media. Bacteria fromsingle colonies were repeatedly grown on
CMC-solid medium and covering the Petri dishes with Congo red dye. The colonies that hadhigh degradation ability then
tested to endoglucanase activity and identifi ed with biochemical and 16S-rRNA tested. The result of thisresearch is there
were two high cellulolytic bacteria isolates: Burkholderia pseudomallei and Klebsiella sp.
Daftar Pustaka :
1. Al-Arif MA, H. Setyono dan Tri-Nurhajati, (2004). Isolasi dan Karakterisasi Ensim Selulase dari Keong Emas dan
Rayap sebagai Bahan Pendegradasi Selulosa. . Surabaya : FKH Unair
2. Anand AAP, Vennison SJ, Sankar SG, Prabhu DIG, Vasan PT, Raghuraman T, Geoffrey CJ and Vendan SE,,
(2010). Isolation and characterization of bacteria from the gut of Bombyx mori that degrade cellulose, xylan, pectin and
starch and their impact on digestion.. - : J. Insect Sci
3. Bakalidou A, P. Kampfer, M. Berchtold, T. Kuhnigk, M. Wenzel and H. Konig, (2002). Cellulosimicrobium variabile
sp. nov., a cellulolytic bacterium from the hindgut of the termite Mastotermes darwiniensis. . - : International Journal of
Systematic and Evolutionary Microbiology
4. Baldani VLD and Doebereiner J,, (1980). Host-plant specifi city in the infection of cereals with Azospirillum spp. . - :
Soil Biol Biochem
5. Barraquio WL, Revilla L and Ladha JK, (1997). Isolation of endophytic diazotrophic bacteria from wetland rice. . - :
Plant Soil
ABSTRACT
Cellulase can degrade cellulose to become glucose. A large number of cellulolytic microbes are available in the animals gut. The
aim of this research was to isolate and identify the cellulolytic bacteria with high activity from Golden snails gut. Some golden snails
were surface sterilized by 70% ethanol and then washed in sterile distilled water. The entire guts were removed from the abdomen
and disrupted then 1 gram of gut debris was suspended in 10 ml sterile distilled water. After serial dilution, aliquots were transferred
into CMC-solid media and incubated at 35° C for 24 hours. The growing cultures were cultivated on NA-solid media. Bacteria from
single colonies were repeatedly grown on CMC-solid medium and covering the Petri dishes with Congo red dye. The colonies that had
high degradation ability then tested to endoglucanase activity and identied with biochemical and 16S-rRNA tested. The result of this
research is there were two high cellulolytic bacteria isolates: Burkholderia pseudomallei and Klebsiella sp.