OPERATING MANUAL

SMART GX
 SMART GX

INTRODUCTION
Introduction
This manual contains a description of the equipment's, installation, operation and maintenance
instruction for the Biotron GX system manufactured by the BIOTRON, INC

DESCRIPTION OF EQUIPMENT
The new developed bioreactor is well designed to provide a stable, reliable bio environment for
various cell culture applications. This system can be employed for batch culture fed-batch culture
or continuous culture with microprocessor control and PC control of pH, DO, agitation,
temperature, antifoam, pump feed, vessel level accurately.
 SMART GX

DESCRIPTION OF PARTS

DESCRIPTION OF VESSEL
The vessels are normally decided according to the working volumes of real media and provide
interchangeable vessel and easy to scale up with various different sizes of vessels from 2.5L, 5L, 7L
and 10L. The glass vessel connected with stainless steel head plate is assembled by simple clamp. It
is a convenient device to assemble into the vessel or to disassemble from one.
There are optional choices between the single wall vessel and double (jacket) vessel.

Single vessel Double vessel Bowl vessel

The stainless steel head plate is composed of 10 location free ports.

Ports are provided in the head plate for the fixed temperature sensor,
Sparger, sampling, pH electrodes, DO electrodes, a foam probe,
exhaust condenser. 4 different tubes of peristaltic pump would be
connected to only one line, which could be space saving in the head
plate.
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DESCRIPTION OF PARTS

Vessel parts and structure

N1 Inoculum Port V1 Heater Plate

N2 Sampling Port V2 Bottom Plate
N3 Sparger Port V3 Packing
N4 DO Sensor Port V4 Support
N5 Thermowell Port V5 Glass Vessel
N6 ColdFinger Port-1 V6 Middle Plate
N7 4P Feeding V7 Top Plate
N8 ColdFinger Port-2 V8 Handle
N9 Condenser V9 DC Motor
N10 PH Sensor Port V10 Bearing Housing
N11 Transfer Line V11 Shaft
N12 Foam Sensor Port V12 Foam Breaker
V13 6th Turbine Impeller
V14 U-type ColdFinger
V15 Hexagon Nut
V16 Top Plate Bolt
V17 Wrench Bolt
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DESCRIPTION OF PARTS
Vessel assembly

7.Thermowell 8.Baffle
3
4
5

9 9.Cooling coil 10.Sparger

10

1 Agitation motor 6 Vessel stand

2 Condenser 7 Thermowell
3 Lifting handle 8 Baffle
4 Headplate 9 Cooling coil (cold finger)
5 Clamping ring 10 Sparger

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DESCRIPTION OF PARTS
Headplate • Vessel cap array (10L more)
• Use blind rubber when you don’t use Foam sensor.
• Foam sensor places other ports easily along the user’s needs.
• Feed line can be changeable in accordance with control
cabinet’s site

Inoculum
Sampling
Exhaust Condenser

Form probe

Sparger DO probe

PH probe

4P feeding

Cooling coil 1 Cooling coil 2

(cold finger 1) Thermowell (cold finger 2)
(Temp Sensor)

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DESCRIPTION OF PARTS

AGITATION SYSTEM
A removable agitation DC motor located on the top of bearing housing is connected to the agitation
shaft. DC motor should be removed before the sterilization and then placed in the bearing housing
after the sterilization. The motor will provide agitation speed rage of 50 to 1200 rpm.
In case of the motor mode, it is controlled by the speed that users set and in case of the DO model, it
is controlled by the measured DO value through the DO sensor to keep the DO value that users set.

DO CONTROL
DO value is controlled in the range of 0 - 100%. DO value is sensed by D.O electrode and also is
controlled and kept by the agitation speed. In case DO value can not be maintained by the agitation
speed, DO should be increased by the pure oxygen supplies by the use of high-pressure oxygen or
oxygen cylinder.

pH CONTROL
pH is controlled in the range of 2.00-12.00. The pH is measured by a glass electrode.
Control is maintained by Aci and Base peristaltic pump by PID

TEMPERATURE CONTROL
Temperature automatically controls itself in the range of 8-80. This Fermentor is able to control the
temperature by the use of circular water-bath and by the heat plate and cooling water of the bottom
part in the single vessel and also in case of bigger vessel than the volume of 10L, cold finger is used
for it.
As the water for controlling the temperature does not pass the body case, it prevent the aging and
corrosion of the controlling plate and also in case f the single vessel, it is possible to control the
temperature by the air cooling. RTD (Resisteance Temperature Detector) is used for temperature
measure.

FOAM CONTROL
Firstly, foam is removed through the foam breaking impeller and then in case of the remaining foam, it
is sensed by foam/level sensor and anti-foam is injected and removes the foam by antifoam pump.

AERATION
Air and oxygen can be supplied to the medium through the ring sparger. The flow rate is controlled
through the control valve, which is located on a flow meter. In case quantities of DO that users want to
get by the agitation is not provided, DO should be increased by the pure oxygen supplies by the use
of high-pressure oxygen or oxygen cylinder.
9.1. AIR SUPPLYING CONTROL
The air flowing amount is controlled in the range of 0-23L/min by the control of a flow meter and the
flow meter suitable for the using vessel is installed. If it requires more air flowing amount, the flow
meter should be replaced.
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DESCRIPTION OF PARTS

EXHAUST SYSTEM
Gas moisture going out to the outside is condensed and then comes back to the vessel. As the gas
goes out through the 0.2 air filter, there is no contamination without the air flowing.

SAMPLING SYSTEM
Sample is collected through the installed sampling line before the fermentation for the sampling of
components during the fermentation. You can use a convenient one of 2 methods following below.
- Close the gas out line, open the sampling line and then do the Sampling by the pressure.
-Connect the silicon tube linked to the sampling line to the peristaltic pump and then do the Sampling
by the pump working.

FED-BATCH SYSTEM
Add a small quantity of the substrate continuously after measuring DO by the added substrate of low
concentration in the early process of fermentation as the indirect post. There are four type of Fed
Batch mode in this system. User can select one of the mode to feed the medium or a special
substrate to the fermenter.
 SMART GX

INSTALLATION PROCEDURE

INSTALLATION POSITION
1. Locate this unit where the air circulation is easy.
2. Install this unit on the place, which has no incline and is stable.
3. Use 220V (110V) main power.
4. Install this unit on the place, which is able to supply the cooling water or temperature controlling
water, or be ready the cooling-water bath.
5. Install the unit on the place providing stable temperature & moisture level.

FERMENTOR INSTALLATION

Vessel:
① Install baffle inside of a vessel.
② Mount the foam breaker onto the shaft of suitable place for the using things. (Fix it a little bit
over the water surface for removing the foam)
③ Install impeller with even distances on the shaft below the foam breaker.
④ Install various sensors (Temperature, pH, DO, Foam sensors) with sealing rubber inspect
whether it is suitable for the orifice on the surface of top plate.
⑤ Connect condenser with rubber holder and connect silicon tube the end of condenser.
⑥ Use vessel clamp to seal between the top plate of vessel and flange.(★ use screwing bolt to
tighten the clamp very slowly and firmly.)

Heating plate
① Locate on the place, which is not too far from the main control case
② Heating line (3 pin) and cooling line (6 pin) connect between port of main case and heating
port.

Main control case:

① Connect the main power supplying line (220V or 110V).
② Connect each motor cable (6 pin), temperature cable (3 pin) and foam sensor cable (2 pin) to
the ports of main case.
③ Install water-supplying line for cooling water and temperature controls.
★ Refers to water supplying line Be careful of safety accidents.
④ Connect the rube of the air pump to the Air in of the main case.
⑤ Check the airflow by operating Air pump and check airflow meter.
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INSTALLATION
Physical location The surface on which you place the SMART GX should be smooth, level
and sturdy. Ensure that the surface can bear the weight of the system
plus vessel contents and any applicable ancilliary equipment.

Also ensure that there is enough space around the back and the front
of the SMART GX for proper operation and access. Allow at least 4
inches of clearance behind the equipment for heat dissipation.

Physical location

least
4inch

Environment The SMART GX fermentor operates properly under the following

conditions:

• Ambient temperature range 10C to 35C

• Relative humidity up to 80% non-condensing

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• Position the SMART GX control station cabinet on a firm, level

Installing surface in an area where utilities are readily available.
the Control
• Connect the mains/power cord to the rear of the control cabinet.
Cabinet At a later time, once the system is completely assembled and all
connections have been made, you will plug the mains/power
cord into a suitable electrical outlet.

Front View

1 Touch screen display

2 Rotameters(from0to4)
1
3 Air out
4 Service connections
5 Pumps(4)
5-1 Pump Swich (4)
2 6 Motor connection
7 Heater/cooler connection

5-1
6

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Installing • Position the SMART GX control station cabinet on a firm, level

surface in an area where utilities are readily available.
the Control
• Connect the mains/power cord to the rear of the control cabinet.
Cabinet At a later time, once the system is completely assembled and all
connections have been made, you will plug the mains/power
cord into a suitable electrical outlet.

Rear View

1 Gas connections
2 Air connections

1 2 3 Cooling vent
4 Cabinet port
5 Overload pump
6 Overload motor
7 Power in
3
8 Power Switch
9 Power out

5 7

6 8

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Heating cooling - Jacketed Vessel Assembly

Front View

1 2 3 4 5 6

1 JACKET OUT 6 WATER OUT

2 JACKET IN 7 CONDENSER OUT
3 CONDENSER IN 8 WATER IN

Heating cooling - Jacketed Connections

Rear View 1
Control Cabinet
Rear
Power out

2
Control Cabinet
Front

Heater/cooler
connection

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Risk of water leaks!

Water and drain Make sure all utility connections have been securely made before
connections connecting to WATER-IN and before turning on the main water supply.

Failure to observe these precautions will result in water leaking out of the
unconnected hoses and the cabinet.

Jacket In & Return

B2 A2
Cooling coil 1 Cooling coil 2
(cold finger 1) (cold finger 2)

B1 A1
JACKET OUT JACKET IN JACKET OUT JACKET IN

Silicone Tubing
1 JACKET IN : A1 – A2
2 JACKET OUT : B1 – B2

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Exhaust Condenser In & Return

filter

Tubing Clamp

C2
Condenser Out

D2
Condenser In

Condenser In
D1 C1
Condenser In Condenser In Condenser Out

Silicone Tubing
1 Condenser Out : C1 – C2
2 Condenser In : D1 – D2

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Main In & Out

F1 E1
Water Out Water In

water drain water supply

Silicone Tubing

1 Water In: E1 – connect water supply

2 Water Out: F1 – connect water drain

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Motor ① Check the connection of coupling between the inside bottom cap of
motor and topside of housing of vessel top plate.
installation ② If the motor is installed on the housing of vessel top plate, there is
5mm of differences. (Refer to the picture following below)
③ If you turn the motor, the differences are disappeared.
④ Tighten the nut located in the bottom side of motor to make sure that
motor does not move.

②→③

Coupling
Coupling disk herb
Coupling
5mm

Motor power Connection

motor

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Sparger Connection

1 Connect the barbed sparger connection here.

filter Connection

tube tube
filter

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Prior to installation, any pH probe you are using should be inspected for
Install pH probe damage, and replaced if necessary.

To avoid damage to the probes during operation, be sure that there is no

interference between the probes and the baffle assembly, impeller blades,
or cooling coil.

1. Wear protective gloves to protect yourself in case of accidental breakage.

2. Lightly coat the pH probe with glycerol.

PH probe

Connect PH probe

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Install thermowell 1. Working from above the head plate, insert the Temperature sensor into
the appropriate port.
(Temperature probe)
2. Finger tighten the knurled adapter

Temperature probe

Connect
Temperature probe

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Prior to installation, any dissolved oxygen probe you are using should be
Install dO2 probe inspected for damage and replaced if necessary.

To avoid damage to the probes during operation, be sure that there is no

interference between the probes and the baffle assembly, impeller blades
or cooling coil.

1. Wear protective gloves to protect yourself in case of accidental breakage.

2. Lightly coat the dO2 probe with glycerol.

dO2 probe

Connect dO2 probe

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Install foam probe 1. Working from above the headplate, insert the foam sensor into the
appropriate port.

2. Finger tighten the knurled adapter

foam probe

Connect foam probe

Fixed to the metal

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Install sampling Refer to the below with the installation

Silicone Tubing

Sampling

Sparger

filter

Clamp A

Clamp B

Clamp C
filter

Sparger
Sampling

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Sampling/harvest 1. Cleaning
method ▪ Clamp B를 열고 A,C를 닫아 공기가 샘플링 라인으로 흘러가게 한다.
▪ 샘플링 라인의 내부가 청소된다.

b
a

2. Sampling/harvest (Sparger)

▪ Clamp C를 열고 A,B를 닫아 공기가 Sparger line 으로 흘러가게 한다.

▪ 내부 공기 압력으로 인하여 Sampling/harvest가 된다

b
a

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Sampling/harvest 3. Sampling/Harvest ( Peristaltic Pump, Syringe )

method ▪ Clamp C를 열고 A,B를 닫는다
▪ sampling line에 연결된 실리콘 튜브를 peristaltic pump 혹은 syringe를 연결하여
샘플링 한다

Peristaltic Pump

Syringe

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• Put each reagent (acid, base, anti-foam, other supply solution) into each
Reagent bottle reagent bottle.
Connection
• Amongst the pipes (2 units) connected to the reagent bottle cap, one is
connected to the air tube by the silicon tube and the other one is
connected to the both parts (the length for the outside of the cap to the
inside of the cap for sinking to the reagent) with the silicon tube. Insert
the both-direct fitting into the end of the silicon tube to make it easy to
connect with the connecting tube of the vessel peristaltic pump after the
sterilization.

• Sterilize on condition that the cap is not closed too tightly but a little bit
opened.

• Close the silicon tube connecting the air filter by the use of clamp for a
tube before the sterilization and then wrap all the air filter with the
aluminum foil not to get wet the inside of the filter during the sterilization.

• Close the silicon tube that both-directs fitting is connected with clamp for
a tube and then wrap the end part with the aluminum foil.

2 3

1 Reagent bottle 3 Peristaltic Pump

2 Filter 4 4P feeding port

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Before you insert tubing into the PUMP CHANNEL , verify that the
Connection PUMP is in the OFF control mode. With reference to the drawing
between below , follow these steps to properly load tubing into the PUMP HEAD
peristaltic pump
and tube Loading Pump Tubing
1 2

6 4
5

1 Upperspring-loadedclamp 4 Pumpcover
2 Pumphead 5 Pumpchannel
3 Pumpheadrotor 6 Lowerspring-loadedclamp

1. Open the PUMP cover to gain access to the interior of the pump.

2. Select the desired tubing size and cut a length ufficient to reach from the
inlet source, through the pump, and to the outlet recipient, allowing a few
extra inches.

3. Form a loop large enough to go around the pump head.

4. Fit one side of the tubing loop into one of the spring-loaded clamps,
pulling the clampopen with your finger.

5. Then, as you rotate the pump’s rotor by hand in a clockwise direction to

clear the channel, lay the tubing in the channel around the pump head.

6. Fit the other end of the tubing loop into the second spring-loaded clamp,
making sure the tubing fits tightly around the pumphead.

7. 7. Press and hold the pump mode Manual button or change the pump
mode to ON at 100% setpoint and ensure that the pump operates
smoothly.

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Gas Connections • Gas inlets are located on the rear panel of the control cabinet. The
sparge outlet is located on the left side of the cabinet.

• There are push-in tube connectors for air, nitrogen, oxygen and
carbon dioxide.

• Insert the tubing into the connection simply by pushing it in. Check to
be sure the connection is secure by pulling gently on the tubing.

1 Air.
2 Gas = O2, N2, CO2.

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STERILIZATION METHOD
STERILIZATION

1. Clean each part of vessel.

2. After extending the baffle, then install it not to follow the shaft turning.

3. Install impeller on the shaft with consideration of the using volume.

4. Install the parts on the top plate of vessel with sealing caution.

5. Change the installation according to the users’ needs. (pH sensor, DO sensor, foam sensor,

6. Sampling line, peristaltic pump connecting line, sparger, air filter and condenser…)

7. In case of the foam sensor, use the port cap (has a small hole on the top of the cap) possible to
insert the folder.

8. In case you use the pH sensor, install it after the calibration.

9. After the calibration, keep the power of controller turning on until you finish to use it.

10. Sterilize by covering the electrode of probe with a cap not to get wet.

11. In case of using the DO sensor, do the calibration after the sterilization.

12. Sterilize by covering the electrode of probe with a cap not to get wet.

13. Install the clamp to seal the vessel and top plate.

14. Be careful of the glass part of vessel not to install by force.

15. Connect by using the silicon tube for the part (sparger, reagent bottle, condenser) that needs
the air filter and also close perfectly the front and back of the air filter with clamp for a tube.
16. In case of using the peristaltic pump, connect the acid, base, anti-foam and peristaltic pump 3
ports (4 ports) with the silicon tube and close each tube with clamp for a tube.
17. Sterilize only each bottle and then it can be used by the connection with each vessel. But after
sterilizing the end of each valve, fix the connector in it for the convenient connection and then
close it with aluminum foil.
18. When the condenser is connected, disassemble the fitting itself that silicon tube is connected
to and sterilize.
19. If there is nothing connected to the top plate, close the rest port with rubber cap for sealing.

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20. In case of the double jacket type, filling half of the jacket with the distilled water is good for the
heat transmission but actually it does not matter that the distilled water is perfectly eliminated for
easy transmission.
21. After the sterilization, in case of closing the opened port and separately sterilizing the medium
after getting cold, add the medium within the bench. When the inoculation inside bench, it does
not need much of caution but when the inoculation in the outside, be careful not to be
contaminated by sterilization. Connect the air line and cooling-water line.
22. Connect the pH, DO, antifoam sensor with the cable.
23. In case of the foam sensor, the one for a folder of the sensor cables should be accurately fixed
in the hole of the port cap.
24. Set the temperature, agitation and aeration.

In the situation that the distilled water fills it, open the top out line not to destroy the vessel by tension.

Do the sterilization
 The condition of sterilization is operated at the time of 121, 20 minute.
 It can be sterilized for a long time by depending on the users.
 When the sterilization is working, open one of the ports no to destroy the vessel by tension.
 When the sterilization is working, it depends on the users’ habits to sterilize the medium in the vessel
together. In case of sterilizing after separating the medium from the vessel by a specific factor,
sterilize the vessel, which is filled with the vessel factors and water except the specific factors and
sensor is closed.

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SENSOR CALIBRATION

a. OPERATING STATUS IN THE CALIBRATION

• It can be calibrated right after the main power on.
• In case the operating status of control panel is ‘RUN’, calibration does not
work so after the calibration of the stop key, press the ‘RUN’ and then it
operates.

b. TEMPERATURE CALIBRATION METHOD

Turn off the main power switch when the unit stops. Turn on the main power
switch, pressing the temperature setting key. In case the temperature value is
different from the real standard temperature value, input the temperature
value of standard thermometer by the up-down control key (do not press the
enter key) and then turn off and on again the power switch. Check the
temperature value on the panel and value of the standard thermometer.

c. FOAM CONTROL METHOD

• Turn the foam tunings controller on the left side of panel to the opposite
direction of clockwise rotation and the sensitivity of water and foam will
increase when it turns to the clockwise rotation.
• With the exception of a special case, as the antifoam does not dwindle, it
does not have to be continuously put into.
• A small quantity of foam is removed through the foam breaker of a physical
method.
• Caution : As the sensitivity reacting according to the type of foam and depth
of sensor touching needs to be controlled, the control for the sensitivity key
also needs. If the foam tunning key turns to the clockwise rotation, the
sensitivity increases.

d. ADDING NUTRIENT METHOD

Supplying nutrient method is following below.
• Automatic feeding method by the DO value (optional specification of
peristaltic pump for feed)
• Automatic feeding method by the pH value (use of pH peristaltic pump)
• Feeding by computer control. (It requires additional devices.)

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e. pH METER CALIBRATION METHOD

① Connect pH sensor to cable.
② Prepare pH buffer solution (7.0, 4.0, 10.0).
③ Stop to operate vessel. (Stop stage)
④ Press pH calibration key.
⑤ Signal of calibration pH 7 is lightened and also pH in the agitation window and cal
signal in the pH window flash.
⑥ Put pH and temperature sensors into 7.0 pH buffer solution and wait until the value is
stable.
⑦ During the calibration of pH 7, the measure value of pH 7 buffer solution shows up
on the pH window.
⑧ If the pH 7 is calibrated, pH 7 is flickering on the pH window and automatically the
lamp of calibration pH 4 is turned on.
⑨ If the lamp of 4.0 turns automatically on, put the pH sensor and temperature sensor
into the buffer solution 4.0 and wait until the value is stable.
⑩ During the calibration of pH 4, the measure value of pH 4 buffer solution shows up
on the pH window
⑪ If the pH 4 is calibrated, pH 4 flickers on the pH window and automatically the lamp
of calibration pH 10 turns on.
⑫ If the lamp of 10.0 turns automatically on, put the pH sensor and temperature sensor
into the buffer solution 10.0 and wait until the value is stable.
⑬ If the calibration of the pH 10 is finished, the indicating lamp is turned off, the ‘END’
flickers on the pH window and then pH calibration is finished.
⑭ Until the fermentation is finished, keep the controller of the fermenter main case
turning on.

☞ On condition of showing the stable values of each pH 4, 7, 10 buffer, if the time

converting into the next step takes long, user can press the calibration set key to
keep going to the next step. Keep your pH electrode with electrode solution or
other solutions to prevent the electrode from drying (solution is provided by pH
sensor company)

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f. DO METER CALIBRATION
① Connect DO sensor to cable.
② Stop the operation of vessel. (Stop status)
③ Set the 500rpm (inputting the motor speed value depending on users) of motor speed.
(During the calibration, the set value of motor speed can be changed at any time)
④ Press the DO Calibration key.
⑤ 0% of calibration DO lamp turns on, the operating motor speed value shows up on the
agitation window and also the dcal indication flickers on the pH window.
⑥ During the calibration of 0%, the ‘0’ value flickers on the DO window.
⑦ If the 0% is automatically calibrated, the 0% lamp turns and also automatically 100%
lamp is turned on.
⑧ During the calibration of 100%, relative DO value, which is read as DO sensor, shows
up on the DO window.
⑨ If the aeration and agitation are supplied most highly, 100% calibration is possible by
pressing the set key once more.
⑩ If the calibration of 100% is finished, ‘END’ indication flickers on the pH window and
the DO calibration is finished.
This Fermentor fixes ‘0’ degree of saltiness inside.

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CONTROL SYSTEM OPERATION • Introduction

A. Function Introduction

B. The Touch Screen

This System Is Developed Touch Screen For Display All Of Data And For The Operation. The User
Can Select The Function Menus And Setup Parameters By Touching The Corresponding Keys On
The Screen.

C. Window Composition
All Of The Windows Are Composed Of The Title Bar, Working Area And The Function Keys. The
Name Of Window Is Displayed In The Title Bar. In Working Area All Of The Operation Data Can Be
Displayed And The Operator Can Select And Set Parameters.

D. Numerical Input
You Can Use The Numerical Input Key Pad To Setup Parameters.

Clicking It, Number Of The Set Value Is Deleted

From Right To Left One By One.

After Clicking It, Message Box Is Disappeared

And It Comes Back To Set Menu.

After Set Value Input, Clicking Enter Key,

Message Box Is Disappeared And It Comes Back
To Set Menu. You Can Confirm The Value Changed Numerical input
In Set Menu Screen.

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CONTROL SYSTEM OPERATION

Main

Turn on the main power, the main screen below is displayed.

◀ Main Screen

Title Bar
BioTron : The company name, click it you can find the product information.
Main : The name of current screen.
Date & Time : Display the current time, click it you can set the date and time.

Working Area
Mode : The name of main parameters of fermentation, click it you can enter to the calibration
process.
S/V : Displays the present set value.
P/V : Displays the present measured value and operating value.
Foam sensitive : It indicate the foam sensor sensitivity, the bigger the number, the more sensitive the
sensor.
Start time : Click it, system starts to record the fermentation time.
Cumulative time : Click it, system stops recording fermentation time.

Menu Bar
Calibration : Select this key, it moves to the Calibration Menu screen.
Setup : Select this key, it moves to the Setup Menu screen.
Control : Select this key, it moves to the Control Menu screen.
Data Log : Select this key, it moves to the Data Log Menu screen.
System : Select this key, it moves to the System screen.
Run and Stop:
Run/Stop : [Run] shows the status that fermenter system is stopped and [Stop] shows the status
that fermenter system is in operation.
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Product information
Touch the [BioTron] in the Main Screen, the Product Information box as below will pop up and show
the product name, version of the current program, the company name and website which developed
this system.

Product information ▶

Date and Time

Touch the [Date & Time] in the Main Screen, the Date & Time Set window as below will pop up.
User can set the date and time by input number using Numerical Input Key Pad.

Date & Time Set ▶

현재시간이 표시된다.

년도 월 일

시간 분 초

Set Value
Touch the [S/V] of each parameter in the Main Screen, the Numerical Input Key Pad will pop up.
The user can input the set value use the Key Pad.
To select the [Foam sensitive] button user can setup the sensitivity of the foam sensor.

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A. Calibration
Select The [Mode] Of Each Parameter, Then Enter To The Corresponding Parameter’s Calibration
Screen.
In This System You Can Calibrate Ph Sensor, Do Sensor, Temperature Sensor And Four Peristaltic
Pumps By This Way. While The Ph Or DO Is Selected, You Can Also Look Over The Recent
Calibration
Information On The Cal Status Screen. Here The DO Is Shown As One Example.

▲ DO calibration Menu ▲ DO calibration status

Click The [Do]key In The Main Screen, Then The Above Selection Window Will Pop Up.
If The [Do Calibration] Selected, The Do Calibration Screen Will Be Displayed And You Can Calibrate
The DO Sensor (Refer To 3.2 DO Calibration).
If The [Cal Status] Selected, The Do Cal Status Below Will Pop Up.

B. Fermentation Time
If The Fermentation Started, You Must Click The [Start Time] On The Main Screen. Then The System
Record Fermentation Time Automatically, And The Time Shows In The [End Time] Button. When The
Fermentation Is Finished, You Can Click The [End Time] Manually To Stop The Time Record.

C. Trend Graph
Click Any Place Of Working Area, The Trend Graph Window Pops Up To Show The Real Time
Recorded Data.

D. Run
Click The [Run] On The Main Screen, The Fermenter System Is Operated. During The Fermenter
Running,
The [Run] Key Changed To [Stop]. Click This Key, The Fermenter Is Stopped.

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 SMART GX

Calibration Menu
Click the [Calibration] on the Main Menu of the Main Screen, the below selection window pops up and you
can calibrate the sensors and pumps etc.
Select one of the items, then you can enter the calibration process of the parameter.

Calibration Menu ▶

pH Calibration

A. Ph Calibration
Get Into The Ph Calibration Process, There Are Three Options, Such As The Normal Ph Calibration, Ph
Recalibration And Cal Status.
The Normal Calibration Can Be Done Using Two Or Above Ph Buffers Before The Fermenter
Sterilization.
In This Fermenter System, User Can Choose The Discretional Two Or Three Buffers Between Ph7, Ph4,
Ph10 Buffer To Do Calibration.
The Ph Recalibration Is Done During Fermentation To Correct The Sensor If Any Uncertainty
Measurement Observed.
Click The [Calibration] On The Main Menu Of The Main Screen, The Below Selection Window Pops Up
And You Can Calibrate The Sensors And Pumps Etc.
Select One Of The Items, Then You Can Enter The Calibration Process Of The Parameter.

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 SMART GX

B. The Normal Ph Calibration

Click The [Ph Calibration] On The Ph Calibration Option Window To Enter The Above Screen.
Then You Can Do The Ph Calibration Following The Below Procedure.
 Put The Ph Sensor Into The Discretional Ph Buffer (Eg. Ph7), Then The Present Measured
Values Display In The Ph, Temp, Mv Cells At The Top Left Corner Of The Screen.
 To Start Calibration, Click The Corresponding Beaker Image Key (Eg. [Ph7]).
 The Calibration Is Done Automatically And The Progress Bar Indicates How Far The
Calibration Has Progressed.
 After Confirming That Currently Measured Value Of Buffer Is Stable, Click The [Nex Cal] To
Finish Calibration Manually.
 Pull Out The Sensor From The Buffer And Cleanse Sensor Glass Part With Clean Water
(Sprayer).Get Rid Of Moisture With Dry Soft Paper Or Cloth.
 Put Ph Sensor Into Another Buffer Solution. The Operating Method Is Same As Above.
 If The Calibration Finished, Click The [Ok] To Exit.
 Click [Cancel] To Cancel It During Buffer Calibration And Exit The Ph Calibration Screen.

▲ pH Calibration Menu ▲ pH Calibration

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 SMART GX

C. Ph Recalibration
Click The [Ph Recalibration] On The Ph Calibration Option Window To Enter The Above Screen.
Then You Can Do The Ph Recalibration Following The Below Procedure.
 The Present Measured Value Displays In The <Process Value>.
 Take Out Some Ferment Medium From The Fermenter.
 Measure The Ph Value Of The Medium Sample Using Other Standard Ph Measure Device.
 Input The Measured Value From The Exterior Device Into The <Recal Set Value> Cell. Then Click
[Cal Start] To Start Calibration.
 If The Calibrating Finished, The Input Value Shows In The <Process Value>cell. Then Click [Close]
To Exit The Ph Recalibration Procedure.

pH ReCalibration ▶

D. Ph Calibration Status
Click The [Cal Status] On The Ph Calibration Option Window To Enter The Above Screen. You Can
Look Over The Recent Ph Calibration Information From It.

pH Calibration Status ▶

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 SMART GX

DO Calibration
Click [DO Calibration] on the Calibration Menu screen to enter the DO Calibration window
as above. Then you can do the DO calibration following the below procedure.
 Before start the DO calibration you must open the air supply, and set the fermentation
temperature and the agitation speed in the Setup screen.
 To start Zero calibration, Click the beaker image key
 [Zero]. In this system the zero calibration do automatically.
 If the present measured value in the top left corner shows 0%, that indicates the zero
calibration finished.
 Click the beaker image key [Sat], to start span calibration. Then the agitation speed and the
temperature reach to fermentation value.
 If the present measured value in the top left corner shows 100%, that indicates the span
calibration finished.
 If the calibration finished, click the [OK] to exit.
 Click [Cancel] to cancel it during buffer calibration and exit the pH Calibration screen.

▲DO Calibration

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 SMART GX

Temp. Calibration
Click [Temperature Calibration] on the Calibration Menu screen to enter the Temperature
Calibration window as above. Then you can do the Temperature calibration following the below
procedure.
 The measured temperature value displays in the <P/V> cell.
 Put other standard temperature sensor to the same solution(or also set the two sensor in air), get
a temperature value.
 Click the [Set temperature input], and input the measured temperature value from the exterior
sensor.
 That value displays in the <P/V> cell.
 Click the [OK] to finish the temperature calibration and exit.
 Click [Cancel] to cancel calibration and exit the Temperature Calibration screen.

▲Temperature Calibration

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 SMART GX

Pump Calibration
Click [Pump Calibration] on the Calibration Menu screen to enter the Pump Calibration window as
above. Then you should select one of the four pumps.
But the calibration procedures of four pumps are the same. And there are two options in pump’s
type. One is the constant speed pump, and another is the variable speed pump.

▲ Pump Calibration Menu

If the pump is constant speed type, the calibration screen is as below.

The calibration procedure is:
 Place one end of the tubing into a beaker filled with water and the other end into a
measuring beaker.
 Complete fill the tubing with water. For this you can manually activate the pump.
 Input the active time to the <active time input>.
 Start the calibration of the pump via [pump on start].
 The pump will automatically stop after the inputted time passed.
 Measure the volume of liquid transferred, and input this value to the <Volume>.
 The system automatically calculates the flowrate considering the inputted active time and
volume and displays the result in the screen.
 Click the [OK] to finish calibration and exit the window.
 Click [Cancel] to cancel calibration and exit the Pump Calibration screen.

If the pump is variable speed type,

the calibration screen is as below

▲ Constant speed pump calibration

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 SMART GX

Gas / Pressure Calibration

a. Gas calibration
(It is an optional function.)

b. Pressure calibration
(It is an optional function.)

◀ Pressure Calibration

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 SMART GX

Setup
Click The [Setup] On The Main Menu Of The Main Screen, The Below Setup Menu Window Pops
Up And You Can Do Setup For Various Parameters.
A. Ph Setup
The Ph Of Fermentation Is Controlled Using The Acid And Base Pump.
Click [Ph Setup] On The Setup Menu Screen To Enter The Ph Setup Window As Above. Click
Each Of The Cells To Set The Ph Set Value, Acid And Base Pump’s On/Off Time.

Setup menu ▲

pH Setup ▶

B. DO Setup
If You Choose The [DO Setup] On The Setup Menu Screen, It Moves To The DO Setup Window.
Then You Can Input The Do Set Value By Numerical Input Key Pad.

C. Temperature Setup
If You Choose The [Temperature Setup] On The Setup Menu Screen, It Moves To The Temperature
Setup Window. Then You Can Input The Temperature Set Value By Numerical Input Key Pad.

D. Agitation Speed Setup

If You Choose The [Agitation Setup] On The Setup Menu Screen, It Moves To The Agitation Setup
Window. Then You Can Input The Agitation Set Value By Numerical Input Key Pad.

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 SMART GX

E. Foam Setup
Click [Foam Setup] On The Setup Menu Screen To Enter The Foam Setup Window As Above. Click
Each Of The Cells To Set The Foam Set Value, Foam Pump’s On And Off Time.
F. Feed Pump Setup
Click [Feed Setup] On The Setup Menu Screen To Enter The Feed Setup Window As Above. Click
Each Of The Cells To Set The Feed Pump’s On And Off Time.

Foam Setup ▲ Feed Setup ▲

G. Pressure Setup
If You Choose The [Pressure Setup] On The Setup Menu Screen, It Moves To The Pressure Setup
Window. Then You Can Input The Pressure Set Value By Numerical Input Key Pad.
H. Alarm Setup
Click [Alarm Setup] On The Setup Menu Screen To Enter The Alarm Setup Window As Above.
In This System You Can Set The Alarms For 9 Parameters, Ph, Do, Agitation Speed, Acid Pump,
Base Pump, Foam Pump, Feed Pump And Pressure.
These Parameters Can Be Selected Optionally And The Condition, Such As Low And High Level,
Should Be Inputted By The Operator.

Alarm Setup ▲
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 SMART GX

Control Menu
Click The [Control] On The Main Menu Of The Main Screen, The Below Control Menu Window Pops
Up. Select One Of The Buttons To Enter Each Control Mode Setup Process. To Exit The Window,
Click The [Close].
A. Fed Batch Setup
Click [Fed Batch Setup] On The Control Menu Screen To Enter The Fed Batch Setup Window As
Above. There Are Four Options In The Fed Batch Setup Screen, Ph Stat, DO Stat, Continuous Culture,
User Feeding, Glucose And Not Use. Select One Of These Modes, And Click The [Setup] To Setup
That Mode.
B. Ph Stat
Select [Ph Stat] On The Fed Batch Setup Screen And Click The [Setup] To Enter To The Below
Window. Description Of Fed Batch Setup-ph Stat Screen:
 Set Point: Ph Set Value
 Active Level: If The [HI] Selected, The Feed Pump Runs When The Current Measured Ph Value Is
Higher Than The Set Point; And If The [LO] Selected, The Feed Pump Runs When The Current
Measured Ph Value Is Less Than The Set Point.
 Delay Time: The Time For The Feed Pump Switch Over.
 Feed Pump: For Set The Pump’s On And Off Time.
 Ph Control Use: Control The Ph Of The Fermentation By Acid And Base Pump While Do The Fed-
batch Culture.

Control Menu ▲ FedBatch Setup ▲

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 SMART GX

C. Ph Stat
Select [Ph Stat] On The Fed Batch Setup Screen And Click The [Setup] To Enter To The Below
Window. Description Of Fed Batch Setup-ph Stat Screen:
 Set Point: Ph Set Value
 Active Level: If The [HI] Selected, The Feed Pump Runs When The Current Measured Ph Value Is
Higher Than The Set Point; And If The [LO] Selected, The Feed Pump Runs When The Current
Measured Ph Value Is Less Than The Set Point.
 Delay Time: The Time For The Feed Pump Switch Over.
 Feed Pump: For Set The Pump’s On And Off Time.
 Ph Control Use: Control The Ph Of The Fermentation By Acid And Base Pump While Do The Fed-
batch Culture.

▲ FedBatch Setup pH Stat

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 SMART GX

D. DO Stat
Select [DO Stat] On The Fed Batch Setup Screen And Click The [Setup] To Enter To The Below
Window.
Description Of Fed Batch Setup-do Stat Screen:
 Set Point: Do Set Value
 Active Level: If The [HI] Selected, The Feed Pump Runs When The Current Measured DO Value
Is Higher Than The Set Point; And If The [LO] Selected, The Feed Pump Runs When The Current
Measured DO Value Is Less Than The Set Point.
 Delay Time: The Time For The Feed Pump Switch Over.
 Feed Pump: For Set The Pump’s On And Off Time.
 Do Control Use: Control The Do Of The Fermentation By Acid And Base Pump While Doing The
Fed-batch Culture.

▲ FedBatch Setup DO Stat

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 SMART GX

E. User Feeding
Select [User Feeding] On The Fed Batch Setup Screen And Click The [Setup] To Enter To The Below
Window.

Screen Description:
 [Current Step(state)]: It Displays The Number Of Current Step That Is In Progress.
 [Step]: It Is The Number Of Feeding Sequence.
 [Time(min)]: It Is The Feeding Time, Every Step’s Time Can Be Set Differently.
 [S/V(ml)]: It Is The Total Volume Of Each Step.
 [Start]: Click This Button, Start The Feeding Step From Step 1.
 [Pause]: Click This Button During The Steps Progress, Stop The Progress.
 [Stop]: Click This Button, Stop The Step Progress, And You Can Restart From Step 1.
 [Close]: If The Setup Is Finished, Click This Button To Exit This Screen.

Operation:
 Input The Each Step’s Time And Total Volume.
 Click The [Start] Button To Start User Feeding Pump Control Mode.
 The System Automatically Implements These Steps According To The Step’s Sequence Number.
 Click The [Pause] Button During The Steps Progress, Stop The Progress. And To Continue The
Step, You Can Click The [Start] Button.
 Click [Stop] Button, Stop The Step Progress, And You Can Restart From Step 1.

▲ User Feeding Pump Control

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 SMART GX

F. Continuous Culture
If The [Continuous Culture] On The Fed Batch Setup Screen Is Selected, The Feed Pump Will
Continuously Feeds The Substrate Or Medium According To The On/Off Time Which Set In The Feed
Setup Screen (Refer To The 4.6 Feed Pump Setup).
G. Glucose
(It Is An Optional Function.)
H. Not Use
If The [Not Use] In The Fed Batch Setup Screen Is Selected, The Feed Pump Not Automatically
Feeds The
Substrate Or Medium.
I. DO Control Mode
Click [DO Control Setup] On The Control Menu Screen To Enter The DO Control Mode Window As
Above. There Are Four Cascade Control Mode In The DO Control Mode Screen, RPM Control, Gas
Control, Air Flow Control And The Press
Control Modes. You Can Click The [Setup] Key To Setup Each Control Mode And In The Right Part
Of The Above Screen Can Set The Control Sequence.

▲ DO Control Mode(Cascade Select)

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 SMART GX

J. DO Control mode Setup

RPM Setup
Screen description:
 [Action Delay Time]: Delay time for the motor switched over.
 [Maximum RPM]: The maximum value that the agitation speed can reach.
 [Increase Step]: Steps in which the agitation speed increases.
 [Increase Time]: The time of speed increasing.
 [Stable RPM Return]: If the RPM increasing can not fulfill dissolved oxygen requirement, then the
 RPM return to the Set Value and the Gas mode is switched.
 [RPM After Gas Insert]: If the RPM increasing can not fulfill dissolved oxygen requirement, then the
RPM maintain the maximum value and the Gas mode is switched.
Operation:
 Set the each parameter using Numerical Input Key Pad.
 Click the [Close] button, the setup box disappeared and it returns to the DO Control Mode window.

▲ DO Control-RPM Setup

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 SMART GX

K. Gas Setup
If the [Gas control] on the DO Control Mode screen is selected and click the [Setup] key, the above
screen pops up.
You can select the kind of gas and input the valve’s on/off time for control the dissolved oxygen level.
L. Air flow Setup : (It is an optional function.)
M. Pressure Setup : (It is an optional function.)
N. Control Sequence Select
If you click the [Control Sequence] from 1 to 4, then the above selection box pops up. You can choose
one of them, then click the [Close] key to return to the DO Control Mode screen.
O. Interface Setup
Click [Interface Setup] on the Control Menu screen to enter the Interface Menu window as above.
There are 9 items in the Interface Menu screen, pH, DO, Temp, Level, Balance, Turbidity,
YSI2700 Glucose Analyzer, Gas Mixer, Aux3.

Control Sequence Select

▲ DO Control-Gas Setup

Interface Menu ▶

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 SMART GX

Data Acquisition and Storage

Click the [Data Log] on the Main Menu of the Main Screen, the below Data Log Menu window pops up.

DataLog Menu ▶

A. Data Log Setup

Click The [Data Log Setup] On The Data Log Menu Window, The Data Log Setup Window Pops Up
As Below. User Can Select The Kind Of Data Logging Devices And The Data Items. The Data Log
Interval Time And The User Name Can Be Setup.

DataLog Setup ▶

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 SMART GX

System
Click [System] button on the menu to advance to [Lock Warning] window where you put ID and
PASSWORD. (As default ID : BIOTRON / PASS : 1945)
※ Caution : System menu involves the setting and operation of the whole system in BIOEXCEL M,
it is prohibited for user to enter into the menu.
Description:
 Click [Diagnostic] to move on to the module test window.
 Click [System Configuration] to move on to system configuration window
 Click [Advanced User Configuration] to move on to the advanced user configuration window
 Click [Close] to exit and return to the main window

▲ Diagnostics Menu ▲ System

A. Diagnostics Menu
Touch [Diagnostic] Button On The <System> To Go To The [Diagnostics Menu]
Description:
 Click [Protocol Test] To Screen Protocol Status Of Each Data Module And Report.
 Click [Temp] To Display First Setup Data In Graph And Temp Related Values,p,i,d,heater And
Cooler, Are Able To Be Set-up.
 Click [Variable/Constant] To Configure Time Interval Of Output Data.
 Click [H/W Port Test] To Test Hardware Of Pump,
 Protocol And Means Of Protocol.
 Click [Motor Test] To Examine The User Configured Inverting Rate And Frequency.
 Click [Flash Test] To Examine Flash Memory.
 Click [Close] To Exit To The Main Window.

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 SMART GX

B. Protocol Test
Touch [Protocol Test] On The <Diagnostic Menu> Screen To Go Advance To The Screen For Testing
State Of Protocol Between CPU And Sensor Board.

Description:
[Gx/Double/Multi/Pc]: Select One Among The Radio Button. Here You Choose The GX.
 Click [Send 1 Packit/Receive 1 Packit] To Send And Receive Packit In 1 Unit.
 Click [Ack Code/Asck Code] To Receive A Text Message Of Ack/Nack Code After Sending Packit.
 Click [Auto Send/Receive] To Make The Protocol Among Modules Correspond Automatically.
The Result Data From Sensor B/D Is Shown In Mv Units And If You Click This Button Again, It Makes
The Auto Send/Receive Stop. When You Can See Variable Mv Value, Nothing Is Failure, This
Function Is For Checking Error In Sensing.
 Click [Remote On] To Help Correspond Data Wirelessly.
 Click [Id] To Change Protocol Channel For Multi Fermenter.
 Click [Close] To Exit This Window.

▼ Protocol Test Screen

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 SMART GX

C. Temp Test
Click [Temp Test] Button On The <Diagnostic Menu> Screen To Go Advance To The Window As
Below. Input Proportional(p), Integration(i) And Derivative(d) Constant Applied Optical Constant. You
Might Not Need To Change These Digit Value Later. This Screen Shows PID Constants As Graph.

Description:
 Kp Const: This Is For P To Increase Temperature Control Rate.
 Ki Const: This Is For I To Increase Temperature Control Rate.
 Kd Const: This Is For D To Stabilize Temperature Into Safety Range.
 As Per Above Constants, You Can Confirm P,i,d And Also Temperature In Temp P/V Cell.
 Input Temperature Value Onto Sampling Time Cell For Configuring Temp.
 Set Temp Value Which You Want To Reach Finally In Temp S/V Cell.
 You Can See Calculated Digit Value On Heater, Cooler Cells As On Or Off Sign.
 Click [Start] Button To See Starting Time, Cumulating Time And Sampling Time, This Button Also
Shows You The Maximum And Minimum Value For Confirmation And Graph Of Test Status.
 Close Pop Screen.

▼ Temperature Test

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 SMART GX

D. H/W Test
Click [H/W Port Test] Button On The <Diagnostic Menu> Screen To Go Advance To The Window As
Below. This Is For Checking If CPU Controls All Of The Functions As Programmed, Generally It Is
Used For Quality Control Under Manufacturing.

Description:
 [Valve]- Test All Of The 16 Valves.
 [Pump]- Test Each Pump’s Condition.
 [Temp]-test The Signal Condition Of Heater And Cooler.
 [Motor]-check Motor’s Condition. It Figures Out If There Is Any Variable Error.

▲ H/W Port Test

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 SMART GX

E. System Configuration
Consisting Menus In [System Configuration Menu] Are Under Development.
Click [System Configuration] Button On The <System> To Go Advance To The Window As Right Side.

Description:
This Screen Which Sets System Properties Is Composed Of Menus For Each Sensor And H/W
System. This Is Optional Menu And Specialized For Connecting Other Programs For Example When
We Connect Other Manufacturer’s Brand Products.

System Configuration ▲

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 SMART GX

F. Advanced User Configuration

Touch [Advanced User Configuration] Button On The <System> Screen To Go Advance To The
Window As Right Side.

Description:
This Menu Is For Meeting Advanced User’s Needs And It Moves On To Each Cascade Menus By
Selecting On The Menu Screen.

Advanced User Configuration ▼

pH Configuration ▲

G. Ph Configuration
 Click [Ph Configuration] To Set Ph Value From The <Advanced User Configuration> Screen.
 This Is Ph Advanced Configuration Window.
 [Offset]- Input An Aberrational Ph Value Which Is Out Of Control By Setting Ph.
 [Calibration Automatic Range]- It Calibrates Ph Range Automatically In Buffer Solution Then The
Result Is Saved.
 [Calibration Temp Compensation]- “Yes” To Select Temp Compensation Option And “No” Not To
Use.
 [Buffer 1] ~ [Buffer 3] – Input Ph Value Of Reagent To Use When Calibrating Ph.
(Ex. “4.0”, “7.0”. “10.0”)

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 SMART GX

H. DO Configuration
Click [DO Configuration] Button On The <Advanced User Configuration> Screen To Go Advance To
The Window As Right Side.

Description:
DO Advanced Configuration Menu.
 [Display Mode] – Select One Among [Do], [O2], [Po2], [Air]
 [Calibration Temp Compensation] : “Yes” To Use Temp Compensation Option And “No” Not To Use.
 [Offset]- Input Do Aberration Value Range From + To –
 [Altitude]- Input Necessary Digit Value For Calculating In Accordance With Altitude Of Do Sensor.
 [Salinity]- Input Necessary Digit Value For Calculating In Accordance With Level Of Salinity.

▲ DO Configuration

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 SMART GX

I. Temp Configuration
Click [Temp Configuration] Button On The <Advanced User Configuration> Screen To Change And
Re-input The Temp Value On It.

Description
 [Temp Control] : Choose The Temperature Control Method
PID Control : Control The Temperature By PID
On/Off Control : Control The Temperature By The Onoff Table Set By The User.
Water Bath : Connects With Water Bath By RS232 Communication And Control By The Water
Bath.
 [Proportional Const]: To Configure Proportional Constant For Controlling Temp, The Higher Constant
The More Efficient In Controlling Temp, However It Can Not Reduce An Aberration. Though It
Reduces An Aberration In Normal Condition, It May Cause The Over Shoot Effect For Much
Response.
(Range: 0.00~100.00)
 [Derivative Const]: It Shows Derivative Constant For Temp Controlling. It Enhanced Stability So That
It Reduces Over Shoot Effect And Much Response. (Range: 0.00~100.00)
 [Integrate Const]: Set The Integral Value For The Temp. Control.
 It Has The Advantage Of Removing Normal Status Error, But It May Also Cause The Functional
Degrade By Much Response. It Causes The Over Shoot Effect By Much Response. (Range:
0.00~100.00)
[Error] : Input The Error(difference) Between The Temperature Setting Value
And Actual One.(A Unit: ℃, Range: 0.00~100.00)
[On Time] : Input The Ontime In One Cycle At The Heating And Cooling
When It Is Above The Relevant Error Value. (A Unit: Sec., Range: 0.00~100.00)
[Off Time] : Input The Offtime In One Cycle At The Heating And Cooling
When It Is Above The Relevant Error Value. (A Unit: Sec, Range: 0.00~100.00)

Temp Control & Sensor Configuration ▶

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 SMART GX

J. Other
Click [Other] Button On The <Advanced User Configuration> Screen To Move On The Window As
Right Side.

Description:
 [Variable-default]: Default All Configured Values.
 [Id&password] For Configuring Screen Lock And System Access
 Agitation Setup For Configuring A Type Of Motor And Hardware Control.
 User Login For Unlocking Screen Lock.
 Vorvertrag Work For Scheduled Operation.
 Remote Control For Pc, Double, Multi Channel.

Advanced User Configuration Other ▼

ID & Password ▲

K. ID & Password
Click [ID & Password] Button On The <Advanced User Configuration Other> Screen To Move On The
Window As Right Side.

Description:
 Input Digit Among 1 To 9 Value As ID And Pass Word, Press SAVE To Save Vice Versa.
 Click [Check Id] To Avoid Using The Same Id With Other Users.

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 SMART GX

l. Agitation Setup
Click [Agitation Setup] button on the <Advanced User Configuration Other> screen to move on the
window as right side.

Description:
 [Maximum RPM] : input the maximum RPM
 [Minimum RPM] : input the minimum RPM.
 [Increase Setup] : input increasing steps as digit value.
 [Step Increase Time] : input the interval time of increasing step.
 [Motor Select] : Geared DC low speed motor is used for controlling low RPM rate with gear motor.
 [Gear Rate] : identify gear rate.
 [Start PWM] : configure initial motor frequency. It help avoid becoming motor rate slow.

Advanced
User Configuration
Agitation ▶

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 SMART GX

M. User Login
Click [User Login] Button On The <Advanced User Configuration Other> Screen To Move On The
Window As Right Side.

Description:
Choose Screen Lock Option If “USER” To Use Or “NOT USER” Not To Use.

Screen Lock Display ▶

N. Vorvertrag Work
Click [Vorvertrag Work] Button On The <Advanced User Configuration Other> To Go Advance To
The Window As Right Side.

Description:
It Asks You Whether You Use Reservation Work, If It Is Set, Vorvertrag Work Button Is Displayed On
Main Screen.

Vorvertrag Work Display▶

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 SMART GX

O. Remote Control
Click [Remote Control] Button On The <Advanced User Configuration Other> Screen To Move On
The Window As Right Side.

Description:
 Click [ID NO] To Input ID
 Click [Control] To Decide To Use [Remote Control] Function.

▲ Remote Control Display

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 SMART GX

P. Analog Data Input/Output

Click The <System> Button At The Bottom Of Main Menu To Go Advance To The <Advanced User
Configuration> Screen Then Touch The <Other> Button To Move On To The <Advanced User
Configuration Other> Window. There Are Many Consists Of The Menu, Select <Analog Input Setup>
Button Among Others To Go To The Window As Below.

Window Description
 Click [A1~A8] To Initialize As Hardware Of 3~4 Variable Pumps For Airflow(mfc), O2 And CO2
 Click [Analog] To Input Analogue’s Max And Min Value.
 Click [Pv Value Setup] To Display Analogue Hardware’s Max And Min Value (%).
For Example, Electronic Analogue’s Sign Is Input In [Analog] Cell And It Results Analogue Data From
Primary Equation.

Operation Description:
 Select A Hardware You Want To Set Up
 Click [Analogue] Cell To Input Your Max And Min Value.
 Click [Pv Value Setup] To Input Max And Min Value.
 Click [Close] To Complete.
[Analogue Input] Is Also Able To Configure From Touching C2 Or Co2 Button On The Left Side Of
The Main Screen.

Analog Input(Output)▶

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 SMART GX

Data Log Software Manual

Main Manu

① Main Menu
② Display for the data and status of the controller
③ Graph, S/V Request and Motor’s Run/Stop

Data Log Manual

This manual is explained to set up the parameter and to send, receive and control the
measured data from the controller to PC during the fermentation.

① Display for the data and status of the controller

This territory is displayed each parameter’s set value(S/V), the present value(P/V) and the status of
the pump, cooler, heater and heater.

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 SMART GX

② Main Menu
[File]
 Graph Save : It is to save the present graph into PC. At first, after clicking the [Data Log Start] in
the [Run] menu, you can save the graph.
 Exit : It is to finish this program.
[Configuration]
 Fermenter Serial Port : It is to set up the transmission speed and number.
 Automatic Graph Save : It is to set up the time for automatically saving the graph.
 Graph Line Width : It is to set up the line width of the parameter’s value displayed in the graph.
 Data Log : It is to set up the interval time to save the transmitted data into the PC during the
data log and user name.
[Setup]
 It is to transmit the data from PC to the controller after setting the each parameter’s set value(S/V),
data and related other value.
[Run]
 Fermenter Serial Port Connect : It is to connect the data communication.
 Data Log Start : It is to start the data log.
 Data Log End : It is to stop the data log.
 Remote On/Off : It is to choose the remote control or not.
 SV Data Request : It is to update each parameter’s set value(S/V) data into the present program
screen.
 Pump Reset : It is to initialize the total accumulated volume of the pump.
[Help]
 About : It shows the information related with data log program.

③ Graph, S/V Request and Motor’s Run/Stop

 The parameter’s P/V that the user wants to monitor can be displayed in the graph territory.
 This function is convenient and efficient because user can choose and monitor.
 The [S/V Request]button’s function is manually to transmit [S/V] value from the controller to the PC.
The [S/V] data inputted in the PC is automatically transmitted into the controller but the inputted
[S/V]data in the controller doesn’t transmit automatically so you need to click the [S/V
Request]button.
 If you click the [Run] or [Stop], the motor of the vessel will work or stop.

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 SMART GX

File Menu

Graph Save

It is to save the status of the present graph to the PC.

This graph is saved at C:\BioTron\BiotronGX1\DataLog\Graph.
Saving is possible when data log is set up after clicking the [Data Log Start] in [Run]menu .

Exit

It is to finish this Data Log program in the PC.

(To finish this program in the PC is not affected at the controller.)

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 SMART GX

Configuration Menu

Fermenter
Serial Port

PICTURE 1

PICTURE 2

If you click the [Configuration]menu and then click the [Fermenter Serial Port], a window pops up for
setting the data communication speed and communication port.

When you to start and operating the program at first, you necessarily need to set up this menu.
Once you set it up, this set value is kept although you rerun this program so you don’t need to set it
up again.

How to set up

Set up the communication speed(Baud Rate) as 115200.

After connecting the communication cable to the PC and checking the connected communication
port (Serial Port) number in the [Device Manager], set up this connected port.
( [My computer]->[Properties]->[Device Manager]-> Confirm the number of the Com port.)

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 SMART GX

<PICTURE 1>
Automatic PICTURE 1
Graph Save

PICTURE 2 PICTURE 3

 This function is to set up the time that is automatically saved the graph in the PC.
 If you click the square box(①) of the blue color, PICTURE 3 is appeared PICTURE 3 is to input
the interval of saving the graph that user wants.
 The standard unit of time is minute(min).
 As follow set up time, the graph in the screen is automatically saved.
 This graph is saved and located C:\BioTron\BiotronGX1\DataLog\Graph in the PC.
 The name of file saved the graph is named as instant saving time.

 If you input the Setting Value as ‘0’, the graph is not automatically saved in the PC.
 If the value is under the Min Value or exceed the Max Value, error message is indicated

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 SMART GX

Graph
Line Width

If you click the [Graph Line Width], you can set up the width of line indicated in the graph .

Data Log

This function is to set up the time of saving the data and the name of the file saved data.
If you click the [Data Log], you can see the two blanks of blue color, “Time Interval(sec)” and
“File Name”.
① Time Interval: If you click the ①, [Count Key Pad] will be appeared. In here, you can set up
the time interval to save the data measured.
The standard unit for setting the time is second(sec).
② File Name Input : If you click the ②, you can name and input the name of the file
automatically made as excel file. If you want to change that, you also revise in here .

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 SMART GX

Setup Menu

PICTURE 1
PICTURE 2

In the [Setup], you can set up the Setting Value(S/V) of the each parameter and other data.
After that, these set up the data is forwarded and applied to the controller.
[pH Control] :
It is to set up the value of the pH and working of the peristaltic pumps for acid and base
(On/off time).
[Foam Control] : If the value(0~5) of sensitivity for foam is high, the sensitivity is also high.
It is to set up the automatic work of the peristaltic pump to eliminate the foam.
[Temp Control] : It is to set up the temperature(℃) and control as basic PID control.
[Agitate Control] : It is to set up the agitation(rpm) for the working of the motor.
This is controlled basic PI control.
[MFC Control]-[Option] : It is to set up the value(%) of MFC for Air Flow.
[DO Control Mode] : To control the DO, the function of Agitate, Gas1 and Gas2 is used.
In the [DO Control Mode], you can set up the value for that.
[Fed Batch Mode] : In the Fed-Batch fermentation, to control the pH and DO, Feed pump is
used.
In the [Fed Batch Mode], you can set up the value for working of Feed pump. You can control
simultaneously with DO Control Mode. If you choose the “User Feeding”, the feed pump is
controlled by the setting time and feeding amount. 74
 SMART GX

Run -> Fermenter Serial Port Connect

<PICTURE 1> <PICTURE 2>

[Run]->[Fermenter Serial Port Connect]

When you click and enter into the [Run] and [Fermenter Serial Port Connect], if the
① Serial Port
is completely set and connected, this pop up indicated as Com1 Port Establish will
appear.
After that, the data communication with controller is ready. The P/V, the value measured
in
the present from the controller is automatically sent and for the sending the setting
value(S/V), you need to click the [Data Request] button.

※ For the working of the data communication, you need to use

the function of the [Remote Control Display] .
(Choose and click the “Use” button)

② If the Serial Port is not exactly connected , this message, “Serial port1 not found”,
is appeared.
<Situation 1.> When the Serial Port is not completely connected,
1. Confirm the connection of the communication cable and whether the cable is connected
loosely
or connected to other port.
2. Confirm the this serial port is already set and worked.
You can check this information in [Control Panel]->[System]->[Device Manager]->[Com3.]
Confirm that the number of the serial ports in the computer is exceed in 1~5 serial ports.
If your computer is not desktop, there is no serial port. Therefore, you need to connect the
extra
USB serial port and use that. In this case, you can add the number of the serial ports until
256
units but in the Biotron GM PC monitoring software, you can just use among 1~5 serial
ports.
If the number of the serial port is exceeded, you need to change the port in [Control Panel]
->[System]->[Device Manager]->[Com]->[Port Setting]->[Advanced] and then use.

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 SMART GX

Run -> Data Log Start

<PICTURE 1>

[Run]->[Data Log Start]

If you click the [Data Log Start], data log starts.

The folder is made from the start time of the data log and the data is saved the excel and
graph file.
Ex) C:\BioTron\BiotronGX1\DataLog\PV\081128172311 - Excel file
C:\BioTron\BiotronGX1\DataLog\Graph\081128172311 - Graph file

[Configuration]->[Data Log]
In the [Data Log], the data(Excel, Graph) is saved by the interval set.
 SMART GX

Run -> Data Log End

<PICTURE 1>

[Run]->[Data Log End]

If you click the [Data Log End], the data log is ended.

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 SMART GX

Run -> Remote ON/OFF

<PICTURE 1>

[Run]->[Remote ON/OFF]

In the [Remote ON/OFF], the remote control of the controller is set up.

* The indication for the situation of the Remote Control *

When the Remote Control is used : It is indicated as ‘Remote ON’.
When the Remote Control is not used: It is indicated as ‘Remote OFF’.

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 SMART GX

Run -> SV Data Request

<PICTURE 1>

[Run]->[SV Data Request]

This function is to request and bring the setting value of parameter from the controller into
the PC program.
The new values from the controller is set up in the computer screen.

The P/V(Present Value) of parameters is automatically send to the PC after connecting the
communication but you necessary need to click the [SV Data Request] button to send the
value of the S/V into the PC from controller.

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 SMART GX

Run -> Pump Reset

<PICTURE 1>

[Run]->[Pump Reset]

The [Pump Reset] initialize the total accumulated volume flew through the peristaltic
pumps(Acid , Base, Feed, Foam)

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 SMART GX

Help -> About

<PICTURE 1> <PICTURE 2>

[Help]->[About]

In the [About], you can confirm the some information for the Product Name, description
for the Biotron GX Fermentation system, version of the program, copyright and engineers
name developed this program.

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 SMART GX

Display for the data and status of the controller

2
3 6
4

7
8

1. Display for the P/V and S/V.

2. D(DO Control Mode), F(Fed Batch Mode) 9 10
Setting situation is displayed as letter.
3. At the working of the fermentor, the image of motor and impeller run together.

4. Cooling Heating

5. Confirmation for the working of the pump.

(At the real working, the image of the pump runs together.)
6. Blinker for the Foam Sensitivity. (Lighting at the above the value)
7. Confirmation of the Remote Control.
8. Confirmation for the working of the fermentor
9. SV data request
10. Fermentor Run/Stop

82
http://www.biotroninc.com

North America
3430 S Broadway, Los Angeles,CA 90007
sales@biotroninc.com

South America
PO Box 653 Belmont, MA 02478 USA
master@biotroninc.com