Biotechnology

1. Used biological processes as a tool to improve human life.

2. Come from an ancient Saxon meaning barley
3. Process in which fluid is sampled, to screen for abnormalities in the developing fetus.
4. Brewed the first beer using barley.
5. Used of heating and enzymes.
6. Biochemical process which transforms sugars and carbohydrates to alcohol.
7. Alcohol percentage that affects bacterial plasma membrane.
8. Found in non-purified beer.
9. Discovered the first bacteria using lens.
10. Name of the first bacteria discovered.
11. Magnification of the first microscope.
12. Described the cells using cork.
13. Cells with no true nucleus.
14. Cells of that contains nucleus and organelles.
15. Source of the first bacteria.
16. Came to Pasteur concerning problems in alcohol distillery.
17. Process of selectively killing bacteria by means of heating.
18. Greek word which means rod.
19. Founding father of microbiology and biotechnology.
20. Father of molecular biology.
21. Father of GMO
22. Fungicide used to lengthen ripening of fruits.
23. Facilitates fermentation.
24. Made an art of wine making.
25. Bacteria that causes holes in cheese which ferments sugar to propionic acid, acetic acid and
carbon dioxide.
26. Acid produced by propionic bacteria.
27. Process of deliberately introducing naked nucleic acids of animal cells to an organism or microbes
to produce products for human use.
28. Hormone that regulates the circulating blood sugar.
29. Organism used to produce human insulin.
30. Insulin for human using bacteria.
31. Involve DNA sequencing and bioinformatics to assemble and analyze the function and structure of
entire genome.
32. Company that produced the first cloned pet animal.
33. Company that produced Dolly.
34. Name of the first cloned animal.
35. Name of the first cloned animal that was sold.
36. Cost of the cloned cat.
37. SNP meaning.
38. Proprietary method of performing multiplex gene expression and genotyping analysis.
39. Developed the first vaccine for rabies.
40. Year microscope was invented.
41. Invented the first microscope.
42. Year when cells was first described.
43. The word biology first appears.
44. Inoculated a child with viral vaccines to protect from small pox.
45. Discovery year of bacteria.
46. Discovery of Escherichia coli.
47. Chromosome theory of heredity.
48. Particle theory of inheritance.
49. Austrian monk that studied Pisum sativum.
50. Discovered sperm in trout.
51. Studied Streptococcus pnuemoniae
52. Ability of the chromosome to absorb stain.
53. Developed the first stain for chromosomes.
54. Fist stain for chromosomes.
55. Discovered chromatin in1883.
56. Discovered the first cancer causing virus in 1911.
57. Tumor cells in connective tissues.
58. Discovered bacteriophages.
59. The word biotechnology was first used by Hungarian agricultural engineer.
60. Discovered the first antibiotic from fungi.
61. Species of fungi used to produce first antibiotic.
62. Name of the first antibiotic.
63. The term molecular biology was coined.
64. Danish microbiologist that used the term genetic engineering.
65. One gene one enzyme theory
66. Molecular disease resulting from the protein molecule hemoglobin.
67. Triple helix structure of DNA
68. Double helix structure of DNA.
69. Discovered DNA polymerase 1.
70. Specific restriction nucleases opening way for gene cloning.
71. A plot using automated DNA squencer
72. Enzyme used for replication
73. Enzyme that causes replication fork.
74. Enzyme that joins replicated DNA fragments.
75. Other term for lagging strand.
76. DNA strand that is continuous and into the replication fork
77. Technology used to male mosquitoes in eradicating zika.
78. Cost per GM mosquito
79. Produce GM mosquito
80. Produced sterilized male mosquito
81. Other techniques aside from SIT.
82. Stage when mosquitoes get irradiated.
83. Signed a bill that requires labelling of GMO products
84. Bill that requires labelling of GMO products.
85. Brain abnormality associated with Zika virus.
86. Fist case of zika virus is at.
87. Genetically engineered plants that uses shortcuts during photosynthesis.
88. Increase percentage of GE tobacco.
89. Enzyme that grabs CO2 molecules.
90. Eats up some of the energy the plants have stored as sugar that reduces yield by 20-50%.
91. First in asia to approve commercial cultivation of GMO crops in 2002.
92. Performs the first successful recombinant DNA experiment.
93. Used for prenatal diagnosis of alpha thalassemia.
94. Show it is possible to introduce specific mutation to specific sites in the DNA molecule.
95. Company that produce human insulin.
96. Created PCR.
97. Used to fight kidney transplant rejection.
98. Company that produce the product of no. 97.
99. Used to regulate the response of the immune system to bacteria, virus, cancer and other foreign
substances that invade the body.
100. Interferon drugs used to treat cancer, kaposi7s sarcoma and complications of AIDS.
101. Human vaccine used for prevention of hepatitis B.
102. Used in treatment of hemophilia.
103. Developed treatment for hepatitis B.
104. Developed treatment for hemophilia.
105. Developed for treating human growth hormone deficiency.
106. Genetically altered bacterium that inhibits frost formation on crop plants.
107. Activase for treat heart attacks.
108. Permitted funding of research for existing stem cells.
109. Lead scientist that created Dolly.
110. Treatment for renal disease anemia.
111. Treatment of white blood cells in chemotherapy patients.
112. Used to replenish WBC count after bone marrow transplant.
113. Treatment for Gaucher’s disease.
114. Treatment for renal cell cancer.
115. Treatment for multiple sclerosis.
116. Genetech’s treatment for growth hormone deficiency.
117. Commercially sold crop engineered to resist rotting.
118. First breast cancer gene
119. Precursor of Vit. A.
120. Rice variety modified to make vit. A.
121. Genetic code of Drosophila melanogaster was published.
122. First ever completed sequence of plant genome.
123. Scientific name of thale cress.
124. 8 giant chromosome of Drosophila melanogaster.
125. Experimented on common fruit fly.
126. First crop to have its genome decoded.
127. First anti-angiogenic drug for cancer.
128. To aid in selecting medications by finding specific gene of the patient that will determine how he
metabolize certain medicine.
129. Product for age-related wet muscular degeneration.
130. Vaccine for cervical cancer.
131. Virus associated to cervical cancer.
132. Patented and trademarked brand of genetically modified fluorescent fish.
133. Source of fluorescent genes.
134. First bitotech pet that hit North America market.
135. Endangered species of wild cattle that was first time.
136. Other animals cloned in 2003.
137. Another name of banteg.
138. Domesticated banteg.
139. Inheritance came from the blood of parents.
140. Mendel’s particle of inheritance.
141. Replaced Mendel’s transmissible factor to gene.
142. Greek word meaning descent
143. Isolated compound called nuclein from pus, kindey, liver, yeast and sperm of salmon
144. Substance resistant to proteases.
145. Miescher renamed nuclein to.
146. Fist US biologist to win Nobel prize.
147. Studied and reconfirm Mendel’s study of peas
148. Colored bodies that could be seen in cell with the use of microscope during mitosis.
149. Indicated that chromosomes were paired and thus could be the source of inheritance.
150. Proposed that mendel’s theory would work if the inherited factors where present in the
chromosomes.
151. Discovered that the amount of the DNA is the same in most cell.
152. Haploid cells
153. Diploid cells
154. Sex cells
155. Somatic cells
156. Contains the chromosome.
157. Conceived of and published the first chromosome map.
158. Demonstrated radiation accelerate mutation rates.
159. Spotted mutation of common fruit fly.
160. Trait linked to y chromosome
161. Trait linked to x chromosome
162. Composed of replicated units
163. Replicated units.
164. Tetranucleotide theory
165. DNA was made up of equal amounts of adenine, guanine, cytosine and thymine.
166. Combination of any of the 4 nitrogen bases.
167. Process of making amino acids from RNA
168. Process of making RNA from DNA
169. Process of making DNA from RNA
170. DNA
171. RNA
172. Carries the codon near the ribosome
173. Provide anti-codon
174. Site where translation occurs
175. Austrian-Irish phycist the word genetic code
176. Book where genetic code was published
177. Also known as genetic code.
178. Embedded in the molecular fabric of chromosomes.
179. N bonds of A and T
180. N bonds of C and G
181. Reason or equal amount of purine and pyrimidine in the DNA
182. Year when Linus Pauling identified the triple helix of DNA
183. Used x-ray in identifying the DNA structure but died before the awarding of nobel prize
184. Two strands of DNA separate first to replicate while being conserved.
185. Found the composition of DNA to be constant within a species but to differ widely between
species.
186. Used streptococcus pneumoniae and proved that DNA is the genetic material.
187. Researched on Streptococcus pneumoniae helped ascertain that DNA is the substance
responsible for heredity.
188. Experimented Neurospora crassa and established each gene in the DNA typically codes for 1
protein.
189. Studies virus and identified DNA to be the genetic material of phages.
190. Small virus that infects bacteria
191. Alfred Day Hershey and Martha Chase named phages as.
192. Double helix unwinds and the original strand of each serve as a template for synthesis of new
strand.
193. Deciphered the first 64 codon in the genetic code by using nucleic acid homopolymers.
194. Experimented who uses uracil as a template.
195. Devised precise and intricate biochemical methods to produce well defined nucleotide in exact
position.
196. Nucleotide triplet that signals a termination of translation.
197. Termination codon or.
198. DNA hydroxyl end
199. DNA phosphate end
200. Y-shaped region where new strands of DNA are elongating
201. Nature of DNA
202. Can only add nucleotides at free _____ end.
203. Bond created by ligase
204. Facilitates joining of replicated DNA strands together.
205. Enzyme for successful replication and provide primer.
206. Received the nobel prize in chemistry in 1993
207. Developed the DNA sequencing.
208. Short strand for DNA/RNA that serves as a starting point for synthesis.
209. PCR.
210. PCR also known as.
211. Melt DNA strands apart at 94 degrees Celsius
212. Step of PCR for 45 sec at 54 degrees Celsius
213. Thermostable DNA polymerase
214. Thermophilic bacteria that was the source of Taq polymerase
215. Enzyme used in RT-PCR
216. The determination of the precise sequence of nucleotide in a sample of DNA
217. DNA sequencing also called as.
218. Developed the method of DNA Sequencing and received Nobel prize in chemistry 1980
219. DNA sequencer based on the method invented by Frederick Sangers.
220. Foreign DNA introduced in a bacterial cell or group of cells which will replicate and increase its
number.
221. Coaxing microbes to produce valuable and unusual products
222. Generating series of DNA fragments from different source with gene sequence of interest.
223. Joining DNA segments to DNA molecule that will replicate
224. Serves a carrier of foreign gene and will replicate the foreign gene and eventually be part of the
bacteria.
225. Recombinant molecule that will replicate and express the desired substance or trait.
226. Specific restriction endonuclease isolated from Escherichia coli.
227. Function for a successful transfer of foreign gene.
228. Collection of containing large fragments where the entire genomic DNA is represented.
229. Defective gene on the phenylalanine hydroxylase
230. Introducing/ transferring copy of gene that is defective to affected individual.
231. Discovered DNA fingerprinting
232. 100% accurate to identify person’s identity
233. Series of identical DNA sequence
234. Transfer of superior genes to recipients
235. Enzyme that disrupts drug’s chemical structure resulting to drug resistance
236. Scientific name of yeast
237. Yeast product against growth deficiency
238. Play and important role in regulation the immune and inflammatory in response to infection
239. Yeast product for pain management
240. Discovered that foreign DNA can be transferred and be part of yeast genome
241. Carlsberg beer yeast
242. Yeast as sugar fungus (Zuckerpilz-Sacchromyces)
243. Fermentation is due to simple chemical reaction.
244. Studied sex life of yeast that lead to identification od best protein processing system
245. Worlds largest milk producer breed of cow
246. Phenomenon of egg with milk
247. IVM-IVF-ET
248. Product of no. 247
249. Product of induced polyploidy in fish
250. Administration of this to peak production increases milk production.
251. Percentage of increased milk production
252. Dolly was cloned using the technology
253. Institute that produce dolly
254. Number of sheep used to create dolly
255. GE animal producing therapeutic proteins in their milk
256. GE animal to reduce waste output from industrial breeding units.
257. GE animal for speeding up industrial fish production
258. Crops genetically modified through the addition of small amount of genetic material from other
organisms through molecular techniques.
259. Soil bacterium used in GMO plants
260. Protein that is specific to lepidopteran insects especially corn borer caterpillars.
261. First public-sector initiated research and first biotech food crop developed in the Philippines.
262. FSBR product
263. Tissue culture is used to produce volumes of cloned plants.
264. Cells capable of producing new individual or clone
265. Greek word meaning branch or shoot.
266. Nutrient jelly treated with pant growth hormone ____ and _______.
267. Cloned animal by Japan using SCNT.
268. Snip, join, shuttle and select

Discussion.
1. Discuss in molecular level the DNA replication
2. Discuss hoe Dolly was made
3. Discuss in molecular level the Protein synthesis
4. Compare DNA from RNA

Enumeration
1. Step of PCR
2. Important gene application
3. Human genetic diseases for which genes are cloned
4. Composition of Nucleotide
5. Type of strand during DNA Replication
6. Enzymes involved in DNA replication
7. Drosophila as a choice of TM Hunt
8. Nitrogen Bases
9. Chargaff’s Rule
10. Three Postulated Methods of DNA Replication
11. Two types of protein
12. Requirements of DNA Replication
13. GE plants
14. GM animals