Romanization
seodulleo on gyeoul seoreowoseo
eoje byeori geuri balganneunji
seodureuneun sigan aswiwoseo
eoje kkumi geuri gireonneunji
[Jinyoung/Minah] saranghago innayo
geudaeui harudeureul
[Jinyoung/Minah] saranghago innayo
geudaeui saramdeureul
[Baekhyun/D.O] nan mideoyo geudae
sojunghan
[Baekhyun/D.O] maeumi mandeureonaen
gijeog
[Yoseob/Hyunseung] geudaega imi hago
isseoyo
[Yoseob/Hyunseung] sesange inneun
geotmaneuro
[Jun.K/Wooyoung] geudaeneun imi
nugungaui
[Jun.K/Wooyoung] Miracle areumdaun
Miracle
oneuldo nugungareul utge hagetjyo
urineun imi algo isseoyo
mueoseul saranghaeya haneunji
geudae nuni danneun modeun
goseseo sijakdoego isseoyo
ama naeireun deo nunbusigetjyo
[Jonghyun/Yonghwa] geudaeraneun iyuro
[Jonghyun/Yonghwa] There can be no
greater love
I am the light of world
You are the light of world
We are the light of world
We’re shining bright together
hoksina sesangi gachi seulpeulkkabwa
goyohido naerineun bitmuldeul
haengbokhaeseoneun an doel
saramcheoreom
eodironga tteomillin saramdeul
modeun geosi eoryeowo
saneun geosi seoreowo
Don’t be afraid my friend
Korean Translation
서둘러 온 겨울 서러워서
Was I sad that the winter was
어제 별이 그리 밝았는지 so rushed
서두르는 시간 아쉬워서 That the stars were that bright
last night?
어제 꿈이 그리 길었는지 Was I sad that I rushed time
That my dream was so long last
사랑하고 있나요 그대의 night?
하루들을 Are you loving your days?
사랑하고 있나요 그대의 Are you loving your people?
사람들을
I believe in the miracle
That your precious heart made
난 믿어요 그대 소중한
마음이 만들어낸 기적 You’re already doing it,
Just by being in the world
You are already someone’s
그대가 이미 하고 있어요 Miracle, a beautiful miracle
세상에 있는 것만으로 You will make someone smile
today
그대는 이미 누군가의
We already know
Miracle 아름다운 Miracle What we need to love
오늘도 누군가를 웃게 It is beginning in all the places
You set your eyes on
하겠죠 Tomorrow will be even more
우리는 이미 알고 있어요 dazzling
Because of you –
무엇을 사랑해야 하는지 There can be no greater love
그대 눈이 닿는 모든
I am the light of world
곳에서
You are the light of world
시작되고 있어요 We are the light of world
아마 내일은 더 눈부시겠죠 We’re shining bright together
그대라는 이유로 In case the world gets sad too,
There can be no greater The rain falls silently
love People have been pushed away
As if they shouldn’t be happy
I am the light of world
You are the light of world Everything is so hard,
We are the light of world Life is so hard
We’re shining bright Don’t be afraid my friend
together I’m your fam Your biggest fan
I believe that your small touch
혹시나 세상이 같이 Can stop tears
슬플까봐
I’m your fam Your biggest fan 고요히도 내리는 빗물들 You’re already doing it,
[Taeyang/Ailee] nan mideoyo geudae jageun Just by being in the world
songillo 행복해서는 안 될 사람처럼 You are already someone’s
[Taeyang/Ailee] meomchul nunmureul 어디론가 떠밀린 사람들 Miracle, a beautiful miracle
You will make someone smile
[Gyuri/Jiyoung] geudaega imi hago isseoyo today
모든 것이 어려워
[Gyuri/Jiyoung] sesange inneun We already know
geotmaneuro 사는 것이 서러워 What we need to love
[Raina/Niel] geudaeneun imi nugungaui Don’t be afraid my friend It is beginning in all the places
[Raina/Niel] Miracle areumdaun miracle I’m your fam Your biggest You set your eyes on
[Raina/Niel] oneuldo nugungareul utge fan Tomorrow will be even more
hagetjyo 난 믿어요 그대 작은 dazzling
urineun imi algo isseoyo Because of you –
mueoseul saranghaeya haneunji 손길로 There can be no greater love
[JYP/Suzy] geudae nuni danneun modeun 멈출 눈물을
goseseo Don’t forget
[JYP/Suzy] sijakdoego isseoyo Through you, light falls on the
그대가 이미 하고 있어요
ama naeireun deo nunbusigetjyo world
geudaeraneun iyuro 세상에 있는 것만으로 Any kind of darkness, in the end
There can be no greater love 그대는 이미 누군가의 Is just a shadow of a moment
Please never forget
itji marayo geudaero inhae Miracle 아름다운 Miracle
sesange naerin bichi isseo 오늘도 누군가를 웃게 You’re already doing it,
[Taemin/Jonghyun] eotteon eodumdo Just by being in the world
gyeolgugeneun 하겠죠 You are already someone’s
[Taemin/Jonghyun] challaui geurimjail 우리는 이미 알고 있어요 Miracle, a beautiful miracle
ppunirangeol You will make someone smile
무엇을 사랑해야 하는지
budi itji marayo today
그대 눈이 닿는 모든 We already know
geudaega imi hago isseoyo 곳에서 What we need to love
sesange inneun geotmaneuro It is beginning in all the places
[Sungchul/Eunji] geudaeneun imi nugungaui 시작되고 있어요 You set your eyes on
[Sungchul/Eunji] miracle areumdaun miracle 아마 내일은 더 눈부시겠죠 Tomorrow will be even more
oneuldo nugungareul utge hagetjyo dazzling
urineun imi algo isseoyo 그대라는 이유로 Because of you –
mueoseul saranghaeya haneunji There can be no greater There can be no greater love
geudae nuni danneun modeun goseseo love
sijakdoego isseoyo I am the light of world
ama naeireun deo nunbusigetjyo 잊지 말아요 그대로 인해 You are the light of world
geudaeraneun iyuro We are the light of world
세상에 내린 빛이 있어
There can be no greater love We’re shining bright together
어떤 어둠도 결국에는
I am the light of world 찰나의 그림자일 뿐이란걸 I am the light of world
You are the light of world You are the light of world
We are the light of world 부디 잊지 말아요 We are the light of world
We’re shining bright together We’re shining bright together
그대가 이미 하고 있어요
I am the light of world Make love ladyrainycorn,
세상에 있는 것만으로
You are the light of world You’re a beautiful miracle, new
We are the light of world
We’re shining bright together
Make love ladyrainycorn geudaeneun
areumdaun gijeok New Babies born
(You already know that)
geudae nuni danneun modeun gose sijagi
dwae
Come on we’ ll light up the world
We’’ re shining bright like the stars
Come together there’’s no greater love
geudaeraneun iyu Yes you are
You are baby light of world
And we gonna shine together
Like diamonds and pearls
geudaega imi hago isseoyo
sesange inneun geotmaneuro
geudaeneun imi nugungaui
Miracle areumdaun Miracle
oneuldo nugungareul utge hagetjyo
urineun imi algo isseoyo
mueoseul saranghaeya haneunji
geudae nuni danneun modeun goseseo
sijakdoego isseoyo
ama naeireun deo nunbusigetjyo
geudaeraneun iyuro
There can be no greater love
그대는 이미 누군가의 babies born
(You already know that)
Miracle 아름다운 Miracle It begins in all the places you
오늘도 누군가를 웃게 set your eyes on
Come on we’ll light up the
하겠죠
world
우리는 이미 알고 있어요 We’re shining bright like the
무엇을 사랑해야 하는지 stars
Come together there’s no
그대 눈이 닿는 모든 greater love
곳에서 Because of you, Yes you are
You are baby light of world
시작되고 있어요 And we gonna shine together
아마 내일은 더 눈부시겠죠 Like diamonds and pearls
그대라는 이유로
You’re already doing it,
There can be no greater Just by being in the world
love You are already someone’s
Miracle, a beautiful miracle
I am the light of world You will make someone smile
You are the light of world
today
We are the light of world
We already know
We’re shining bright
What we need to love
together
It is beginning in all the places
You set your eyes on
I am the light of world
Tomorrow will be even more
You are the light of world
dazzling
We are the light of world
Because of you –
We’re shining bright
There can be no greater love
together

Make love ladyrainycorn

그대는
아름다운 기적 New Babies
born
(You already know that)
그대 눈이 닿는 모든 곳에
시작이 돼
Come on we’ll light up the
world
We’re shining bright like
the stars
Come together there’s no
greater love
그대라는 이유 Yes you are
You are baby light of world
And we gonna shine
together
Like diamonds and pearls

그대가 이미 하고 있어요
세상에 있는 것만으로
그대는 이미 누군가의
Miracle 아름다운 Miracle
오늘도 누군가를 웃게
하겠죠
우리는 이미 알고 있어요
무엇을 사랑해야 하는지
그대 눈이 닿는 모든
곳에서
시작되고 있어요
아마 내일은 더 눈부시겠죠
그대라는 이유로
There can be no greater
love
Materials Required:

 Bacterial suspensions in broth: With a sterile

cooled loop, place a loopful of the broth culture on
the slide. Spread by means of circular motion of
1. Clean glass slides the inoculating loop to about one centimeter in
2. Inoculating loop diameter. Excessive spreading may result in
3. Bunsen burner disruption of cellular arrangement. A satisfactory
4. Bibulous paper smear will allow examination of the typical cellular
5. Microscope arrangement and isolated cells.
6. Lens paper and lens cleaner
 Bacterial plate cultures: With a sterile cooled loop,
7. Immersion oil
place a drop of sterile water or saline solution on
8. Distilled water
the slide. Sterilize and cool the loop again and pick
9. 18 to 24 hour cultures of organisms
up a very small sample of a bacterial colony and
gently stir into the drop of water/saline on the slide
to create an emulsion.
 Swab Samples: Roll the swab over the cleaned
Reagents: surface of a glass slide.

1. Primary Stain - Crystal Violet Please note: It is very important to prevent preparing thick,
2. Mordant - Grams Iodine dense smears which contain an excess of the bacterial
3. Decolourizer - Ethyl Alcohol sample. A very thick smear diminishes the amount of light
4. Secondary Stain - Safranin that can pass through, thus making it difficult to visualize
the morphology of single cells. Smears typically require only
a small amount of bacterial culture. An effective smear
appears as a thin whitish layer or film after heat-fixing.
Procedure:
Part 4: Heat Fixing
Part 1: Preparation of the glass microscopic slide
Heat fixing kills the bacteria in the smear, firmly adheres
Grease or oil free slides are essential for the preparation of the smear to the slide, and allows the sample to more
microbial smears. Grease or oil from the fingers on the readily take up stains.
slides is removed by washing the slides with soap and
water. Wipe the slides with spirit or alcohol. After cleaning,
dry the slides and place them on laboratory towels until
ready for use.  Allow the smear to air dry.
 After the smear has air-dried, hold the slide at one
end and pass the entire slide through the flame of
a Bunsen burner two to three times with the
Part 2: Labeling of the slides smear-side up.

Drawing a circle on the underside of the slide using a
glassware-marking pen may be helpful to clearly designate
the area in which you will prepare the smear. You may also
label the slide with the initials of the name of the organism
on the edge of the slide. Care should be taken that the label
should not be in contact with the staining reagents.
Now the smear is ready to be stained.
Please Note: Take care to prevent overheating the slide
because proteins in the specimen can coagulate causing
cellular morphology to appear distorted.
Part 5: Gram Stain Procedure

1. Place slide with heat fixed smear on staining tray.

Part 3: Preparation of the smear
 2. Gently flood smear with crystal violet and let stand 7. Wipe the glass slide with spirit and wave the slide
for 1 minute. over the Bunsen burner to remove any unwanted
microorganisms in the slide.
3. Tilt the slide slightly and gently rinse with tap water 8. Label one side of the glass slide with
or distilled water using a wash bottle.
1. Your initials
4. Gently flood the smear with Gram’s iodine and let 2. The date
stand for 1 minute. 9. While flaming the inoculation loop be sure that
each segment of metal glows orange/red-hot
5. Tilt the slide slightly and gently rinse with tap water before you move the next segment into the flame.
or distilled water using a wash bottle. The smear will 10. Once you have flamed your loop, do not lay it
appear as a purple circle on the slide. down, blow on it, touch it with your fingers, or
touch it to any surface other than your inoculums.
6. Decolorize using 95% ethyl alcohol or acetone. Tilt If you do touch the tip to another surface or blow
the slide slightly and apply the alcohol drop by drop on it, you will have to re-flame the loop before you
for 5 to 10 seconds until the alcohol runs almost proceed to your experiment.
clear. Be careful not to over-decolorize. 11. Allow your loop to cool before you try to pick up
your organism. If you pick up organism with a hot
7. Immediately rinse with water. inoculation loop, your cells will be killed and will
affect your results.
8. Gently flood with safranin to counter-stain and let 12. When removing the caps from tubes, always keep
stand for 45 seconds. the caps in your hand. Never set them on the
table, as they could pick up contaminants.
9. Tilt the slide slightly and gently rinse with tap water 13. Always handle open tubes at an angle near to the
or distilled water using a wash bottle. flame of the burner; never let them point directly
up, since airborne or other environmental
10. Blot dry the slide with bibulous paper. organisms could fall into the tube and cause
contamination.
11. View the smear using a light-microscope under oil- 14. As soon as you transfer the organism into the slide,
immersion. flame your loop. Never place a contaminated tool
on your workbench.
15. Try to prepare a single cell layer of organism (a thin
smear). Otherwise the all cells will appear as gram
Differences Encountered in a Real laboratory: positive in thick area.
16. Do not overwarm the cells. Dry the slide
thoroughly prior to heat fixing.
17. Use young, vigorous cultures rather than older
cultures for your experiment.
In an actual laboratory setting, there are certain important
18. Decolorisation step should not exceed the time
steps that are not necessarily applicable in a virtual lab:
limit.
19. While washing the slide after staining, do not let
the water stream fall directly on the smear. This
may disrupt the smear. Let the stream of water
1. Always wear gloves, and lab coat. flow slowly along the surface, such that only the
2. Tie your hair properly to prevent any stain is flooded and the smear is intact.
contamination from the culture you are working 20. Always prefer to observe under 10X first. This will
with. give you an idea of the location of a good area for
3. After entering the lab, make sure that the observation. After this you may prefer to switch
microscope is working properly. Oculars and over to 40X.
objective lenses should be cleaned before and 21. Do not ever observe at a specimen at 100X without
after each use with lens paper. oil.
4. Adjust the illumination before using the 22. While focusing the microscope, glass slides should
microscope. be handled carefully to avoid the chance of
5. Prepare your work space (Laminar Air Flow chipping or breaking.
Cabinet) or lab bench by wiping down the area 23. After the observation, wipe the microscopic lens
with disinfectant. with an absorbent paper and cover the microscope
6. Properly adjust the flame of the Bunsen burner. properly.
The proper flame is a small blue cone; it is not a
large plume, nor is it orange.
 24. Discard all contaminated materials properly and
return your supplies to the proper storage
locations, and clean up your working area.
25. Always disinfect your work area when you are
finished.
26. Ensure proper hand washing before you leave
from the laboratory.
Typical Gram-negative bacteria:
1. Bordetella pertusis, the causative agent of
whooping cough
2. Salmonella typhi, the causative agent of typhoid
3. Vibrio cholera, the causative agent of cholera
4. Escherichia coli, the normally benign, ubiquitous,
gut-dwelling bacteria
Typical Gram-positive bacteria:
1. Staphylococci such as Staphylococcus epidermidis
and Staphylococcus aureus which is a common
cause of boils.
2. Streptococci such as the many species of oral
streptococci, Streptococcus pyogenes which causes
many a sore throat and scarlet fever and
Streptococcus pneumoniae which causes lobar
pneumonia.
3. Clostridia such as Clostridium tetani, the causative
agent of tetanus (lockjaw).
4. Actinomyces such as Actinomyces odontolyticus
which is found in mouth.
5. Species of the genus Bacillus such as Bacillus
subtilis which are common microbes living in soil.
Generally cocci are Gram-positive but there are exceptions.
The most significant from a clinical point of view is the
gonococcus, Neisseria gonorrhoea which typically appears
as a Gram-negative diplococcus looking very much like a
pair of kidney bean.