Adventist University of the Philippines

Medical Laboratory Science Dept.

Introduction to Hemostasis Testing

NAME: ___________________________________ Lab Schedule _______________ Date _________

I. Basic Hemostasis
1. Definition of terms:
a. Hemostasis

b. Coagulation

c. Fibrinolysis

d. Fibrinogen

e. Fibrin

f. Plasminogen

g. Plasmin

2. What are the three stages of hemostasis and the end product for each stage?
a.
b.
c.
3. Draw the three stages of hemostasis.

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 Adventist University of the Philippines
Medical Laboratory Science Dept.
Introduction to Hemostasis Testing
II. Tests for Evaluation of Hemostasis

What specific Specimen

Which stage of component of the stage Used (Indicate
Lab Test recommended
hemostasis is tested for? of hemostasis is this test
for? anticoagulant)
performed on
Number and function of
Bleeding time Primary hemostasis a standard
platelets
wound
Capillary fragility
test

Platelet Count

Platelet
Aggregation test
Clotting
time/Coagulation
time (Lee &
White)

Clot retraction

Clot lysis time

Prothrombin Time

Activated Partial
Thromboplastin
Time
Fibrin Stabilizing
factor

Fibrinogen assay

D-dimer test

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 Adventist University of the Philippines
Medical Laboratory Science Dept.
Introduction to Hemostasis Testing
III. Specimen Collection and Handling in Hemostasis Laboratory
1. Describe the ideal way of collection venous blood for coagulation tests.

2. Why is it important to adjust the volume of citrate based on the patient’s hematocrit?

3. Compute for the following: (show the formula you used, your computation and cite the
source of your formula)

a. Volume of citrate required for 5 mL of blood from a patient with a hematocrit of

0.20 L/L

b. Volume of citrate required for 5 mL of blood from a patient with a hematocrit of

0.40 L/L

c. Volume of citrate required for 5 mL of blood from a patient with a hematocrit of

0.60 L/L