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SERVICE MANUAL
COD. 610056_14
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FULLY and FULLY SMART Service Manual
TABLE OF CONTENTS
1. INTRODUCTION............................................................................................................. 5
2. INSTRUMENT’S DEVICES DESCRIPTION ................................................................... 6
2.1 Liquid Processing System ........................................................................................................................ 6
2.1.1 Well Selector...................................................................................................................................... 7
2.1.2 Pipetting System ................................................................................................................................... 7
2.2 Reading Group ......................................................................................................................................... 7
2.4 Application Program ................................................................................................................................. 8
3. DESCRIPTION OF THE ELEMENTS ............................................................................. 9
3.1 Well Selector ............................................................................................................................................ 9
3.1.2 Rotor Gear ......................................................................................................................................... 9
3.1.3 Pre - Warming Of The Reaction Well .............................................................................................. 10
3.2 Diluter ..................................................................................................................................................... 11
3.3 Transfer Arm........................................................................................................................................... 12
3.3.1 Arm .................................................................................................................................................. 13
3.4 Sipping System....................................................................................................................................... 15
3.5 Optical System ....................................................................................................................................... 16
3.5.1 Filter Equalization................................................................................................................................ 17
4. ELECTRONIC CIRCUIT ............................................................................................... 19
4.1 Block Diagram............................................................................................................. 19
4.2 Electronic Board placement ................................................................................................................... 22
5. LAYOUT AND CALIBRATIONS .................................................................................... 26
5.1 Ordinary / Occasionally Maintenance..................................................................................................... 26
5.2 Extraordinary Maintenance .................................................................................................................... 27
5.2.1 Service ............................................................................................................................................. 27
5.2.2 Layout And Calibration Menu .......................................................................................................... 28
5.2.2.1 Reagent layout.............................................................................................................................. 29
5.2.2.2 Sample layout ............................................................................................................................... 30
5.2.2.3 Reaction tray layout ...................................................................................................................... 31
5.2.2.4 Washing well layout ...................................................................................................................... 32
5.2.2.5 Sensor testing............................................................................................................................... 33
5.2.2.6 Temperature Adjusting ................................................................................................................. 34
5.2.2.7 Pre – Amplifier Board calibration ................................................................................................. 37
6. MAINTENANCE ............................................................................................................ 40
6.1 Disconnecting Suction and Dispensing Flexible Tubes ......................................................................... 40
6.2 Disassembling the transfer arm............................................................................................................. 40
6.3 Changing the casing............................................................................................................................... 42
6.4 Changing the main board ....................................................................................................................... 43
6.5 Changing the optical group .................................................................................................................... 43
6.6 Changing the filter wheel........................................................................................................................ 44
6.7 Changing the filter .................................................................................................................................. 45
6.8 Changing the filter wheel motor.............................................................................................................. 45
6.9 Changing the peristaltic pump................................................................................................................ 45
6.10 Changing the peltier cell....................................................................................................................... 45
6.11 Changing the photodiode ..................................................................................................................... 45
6.12 Changing the fan .................................................................................................................................. 46
6.13 Changing the temperature sensor........................................................................................................ 46
6.14 Changing the lamp ............................................................................................................................... 46
6.15 Changing the lens ................................................................................................................................ 47
6.16 Changing the transfer arm vertical motor ............................................................................................. 47
6.17 Changing the transfer arm rotation motor ............................................................................................ 48
6.18 Changing the rotor motor ..................................................................................................................... 49
6.19 Changing the diluter motor ................................................................................................................... 49
6.20 Changing the pre-warming device of the reaction well ........................................................................ 50
6.21 Changing the safety spring................................................................................................................... 51
6.22 Changing the needle set ...................................................................................................................... 51
6.23 Changing the pre-warming device of the reagent ................................................................................ 51
6.24 Lubrication ............................................................................................................................................ 51
7. CARE AND CLEANING ................................................................................................ 53
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7.1 Cleaning of the optical components (lamp, lens, filters and photodiode)............................................... 53
7.2 Cleaning of the filters.............................................................................................................................. 53
7.3 Cleaning of the lens................................................................................................................................ 53
7.4 Cleaning of the photodiode .................................................................................................................... 53
7.5 Cleaning the flow-thru cuvette................................................................................................................ 54
7.6 Cleaning Aspiration Circuit ..................................................................................................................... 54
7.7 General cleaning of the instrument ........................................................................................................ 55
7.8 Preventive maintenance......................................................................................................................... 55
APPENDIX 1 ..................................................................................................................... 56
APPENDIX 2 ..................................................................................................................... 59
APPENDIX 3 ..................................................................................................................... 61
TUBING ........................................................................................................................ 61
APPENDIX 4 ..................................................................................................................... 62
TROUBLESHOOTING ...................................................................................................... 62
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RELEASE HISTORY
1. INTRODUCTION
This manual contains all the informations about the automatic analyzer FULLY and it could be considered a
training document for the Technical Assistance Service and a reference guide for repair and maintenance.
This manual is divided in three parts: the first one is about the description of mechanical groups, the second
one is about the configuration of the instrument and the third one is about the electronic circuits.
This manual is valid for FULLY and FULLY SMART analysers. FULLY SMART analyser is not provided with
display and internal PC: the software, which is the same for FULLY and FULLY SMART, should run on a
external PC which should be connected to the instrument via serial RS232 port. The connection between
external PC and the internal printer is achieved through a parallel port.
FULLY is an instrument that automatically performs clinical chemistry tests, by mixing samples and reagents
and then measuring their absorbance. It has been designed as a processing and reading unit, connected to
an internal computer (external for FULLY SMART) where the application runs.
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FULLY and FULLY SMART consists in a liquid processing systems that pipettes the reagents from their
bottles and the samples from their wells, and mixes them up in the reaction wells where the reaction takes
place. The reaction wells are warmed up in order to have the reaction mixtures at the temperature near to
that of the reading cell.
The system is made up of in some groups: peristaltic pump, diluter, transfer arm, optical group, rotor gear,
reagent plate.
All of these groups (except reagent plate that is fixed) are controlled by an electronic system with a
microprocessor, by means of the corresponding power circuits. The microprocessor is linked with the
internal (or external) pc that contains the application program with all the needed tools (programming of
tests, working lists….)
It is not the aim of this manual to describe the way this program works (for detailed information about the
program operation, refer to the User’s Manual), but only the parts required for the maintenance of the
instrument will be considered.
As shown in the diagram, FULLY has four main systems that will be explained in the follow chapters:
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This system is in charge of carrying the reaction mixture from the reaction well into the flow- thru cuvette,
where it is precisely thermostated and its absorbance read.
The reaction mixture is removed from its well by means of the suction needle and, through the peristaltic
pump, delivered into the cuvette. For kinetic measurements where readings take place at programmed time
intervals in which temperature should be kept stable, the cuvette is thermo- regulated by means of the peltier
cell.
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In order to avoid the presence of bubbles inside the cuvette, that could interfere with reading, the optical
block is assembled with a 10° angle, so that any eventual bubbles produced along the circuit and retained by
the cell, are quickly removed.
All these elements are controlled by means of electronic boards, including a microprocessor with a program
able to handle them. This program receives from the internal PC detailed instructions of the diverse steps to
be performed.
http:\\www.biosys.it\software
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The rotor gear allows the positioning of the sample and reaction wells below the arm needle.
1. Rotor support
2. Base
3. Axle pulley
4. Driving belt
5. Driving pulley
6. Stepping motor
7. Home sensor
8. Stirrup
9. Rotor axle
10. Centering support
11. Dowel pins
12. Knob
The centering support is moved by a stepping motor that steers the movement of driving pulley, driving belt,
axle pulley and rotor axle. All the systems is fixed on the base and the base is fixed on the rotor support.
Inside of the support the are two bearings that allow the rotor axle rotation.
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On the centering support there are two dowel pins that allow a correct positioning of the sample/reaction
plate. The centering support is fixed on the rotor axle through a screw and the sample/reaction plate is fixed
on the centering support by a knob.
In order to monitoring the position of the rotor, there is an home sensor fixed on the base and a stirrup fixed
on the axle pulley. Using this reference, the mechanism moves forward a definite number of steps till
reaching the established position.
Around the anodized-aluminum pre-warming box there is a resistor wire that warms up the walls of the box.
The reaction wells are positioned inside of the box and they reach the temperature close to that of the walls.
Under the pre-warming box there is a box cover that protects the rotor gear from the dusty and the liquid
leakage.
This group is fixed to the rotor gear base by four box support.
The temperature is controlled by a temperature sensor and there is a safety bimetallic thermostat that
switches off the current intensity only if the temperature achieve 50°C.
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3.2 Diluter
The diluter allows to aspirate and dispense samples and reagents into the reaction wells.
1. Syringe
2. Valve
3. Inlet hole
4. Outlet hole
5. Special screw
6. Guide axle
7. Revolving screw
8. Plunger displacer
9. Home sensor
10. Position plate
11. Supporting plate
12. Motor
13. Belt
14. Axle pulley
15. Driving pulley
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The syringe and the e-valve are connected to the dispensing needle and to the washing bottle by tubing
attached to the corresponding connector. The right connector is different from the left one.
The syringe is connected to the plunger displacer by a special screw, and the plunger displacer is moved by
the system driving pulley- belt- axle pulley, moved by a stepping motor.
The maximum volume in the syringe is controlled by the position plate that stops the syringe when it goes
through the home sensor.
The diluter is fixed directly on the vertical diaphragm and it is in vertical position in order to avoid the
presence of bubbles inside the syringe. In this way, any eventual bubbles produced and retained by syringe,
are quickly removed.
NOTE:
The join that are screwed to e-valve are weak. So excessive strength on
fixing the plastic join to e-valve will damage the e-valve permanently.
Note that you should fix these joints by hands, or if you are using tools
pay attention on doing this.
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3.3.1 Arm
1. Arm support
2. Centering box
3. Arm screw
4. Tube in
5. Tube out
6. CS
7. Reagent warming case
8. Reagent warming box
9. Spacer
10. Home sensor stirrup
11. Spring fixation plate
12. Home sensor
13. Spring
14. Needles holder
15. Suction needle
16. Dispensing needle
17. Level sensor connector
18. Arm axle
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3.3.2 Rotor
19. Support block
20. Guides
21. Safety Spring
22. Arm displacer
23. Stepping Motor for the arm rotation
24. Motor pulley for the arm rotation
25. Encoder plate
26. Axle pulley for the arm rotation
27. Belt for the arm rotation
28. Axle bearings
29. Belt block
30. Belt for the up and down movement
31. Axle pulley for the up and down movement
32. Motor pulley for the up and down movement
33. Stepping motor for the up and down movement
34. Bearings group
35. Vertical Home sensor stirrup
36. Home sensor for the up and down movement
37. Rotation Home sensor stirrup
38. Home sensor for the arm rotation
All this group is assembled on a support block that consists in 2 parts: a principal holder part and two guides.
The arm displacer is moved along these guide by a pulley- belt- motor system. The belt is fixed to the arm
displacer by a belt block. With this movement, the arm can introduce the needles in the wells.
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To regulate the highness of the arm, on the arm displacer there is a home sensor that allows to stop the
group when vertical home sensor stirrup pass trough the home sensor (upper position).
To preserve the needles and to avoid the downwards displacement when you disconnect the instrument,
there is a safety spring around the left guide.
The arm can rotate because of pulley- belt- motor system that it is on the arm displacer. There is a double
tree motor that moves the pulley on the back side and it moves the encoder plate on the up side. The
encoder picks the movement impulses corresponding to the number of the steps of the motor.
In case of blocking of the rotation, there will be a discrepancy in this number and the system alerts on a
possible obstacle.
On the right side of the arm displacer, there is a home sensor that stops the rotation of the arm when the
Rotation Home sensor stirrup pass trough the home sensor. There is a mechanical stop on the home sensor
stirrup that allows to stop the rotation even if there is an error. On the other side, the right guide is the other
mechanical stop.
On the arm support there is a centering box that is fixed on the arm axle by an arm screw.
On the arm support there is a Printed circuit and, on this one, there is a Reagent warming box that allows to
pre- warm the reagent inside of the tube because inside of this box there are two heating resistor. Inside of
this box there is also a temperature sensor to keep the temperature stable.
The Reagent warming box with the tube turned around, is covered by a Reagent warming case.
There are two tubes: one, connected to flow cell, is connected into the suction needle, the other one,
connected to the diluter and turned around the reagent warming box, is connected into the dispensing
needle. Both of needle are fixed on the Needles holder and tightened by two screws. They are connected to
the sensor level by a wire. The needles holder is mobile and is fixed into a conical lodging by a spring. The
spring fixation plate and the spacers fix the spring by its upper side. The home sensor stirrup, that works
together with the some sensor is fixed to the holder. If when lowering the arm the needles find any obstacle,
the plate moves out from the center of the home sensor and this sends the blocking signal.
The needles, made of stainless steel, are parallel and have same length.
The arm circuit is connected to the electronic board by the socked.
The cover is fixed on the arm displacer by three screws.
On the cover there are two led, the red one on the left side for the impact, and the green one on the right
side for the level sensor.
The sipping system allows to carry the reaction mixture from the wells and to delivery the mixture to the flow–
cell cuvette.
1. Washing bottle
2. Diluter
3. Transfer arm
4. Needles
5. Flow- cell cuvette
6. Peristaltic pump
7. Waste bottle
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The sample is sipped by the suction needle and delivered to the cuvette. The tubes between needle and
cuvette is made by teflon and it is covered and protected by a silicon tube. It has a specified length on which
the instrument is calibrated. Each time this tube is changed, the corresponding adjustment of the pump must
be performed.
When the sample is inside of the cuvette, the reading is performed.
After the reading, the peristaltic pump aspirate the sample through a tefllon tube covered by a silicon tube
and it delivers the sample into the waste bottle through a tygon tube.
The peristaltic pump consists in six- roller rotor and it is moved by a stepping motor. The peristaltic pump
tube is a special rubber tube and it is fixed instead to be parallel each other.
If you prefer to use a external tank, there is a connector on the vertical plate of the instrument. The waste
tygon tube has to be fixed on this connector and on the back side of the instrument there is another blue
connector on which the external tank tube is to be fixed.
On the two bottles, washing and waste, there is a level sensor that alert the operator when the washing
bottle is empty and when the waste bottle is full. When you have to extract the bottles, you have to take off
the gold connector in order to switch off the level sensor.
1. Incubator block
2. Conductor pad
3. Peltier cell
4. Dissipator
5. Tilt base
6. Fixing screw
7. Temperature sensor
8. Isolator
9. Halogen lamp
10. Lamp support
11. Filter wheel
12. Filter wheel support
13. Stepping motor
14. Photodetector
15. Photodetector protection
16. Incubator fan
17. Incubator fan support
18. Lent
19. Lents holder
The incubator is a thermostating system, it is designed in order to keep the temperature inside the flow- cell
at the programmed value and inside a precision range (+/- 0.2°C).
The cuvette is positioned into the cuvette holder that is covered with a conductor pad, in order to guarantee a
homogeneous temperature distribution along the cuvette. A special screw fixes the cuvette inside of the
incubator.
The incubator block is in contact with one of the sides of the Peltier Cell and it is connected with the
dissipator by four insulated screw. The other side of the Peltier Cell is in contact with the dissipator. The
Peltier Cell pumps heat from one side to the other according to the direction of the current flow.
A power control circuit is in charge of guiding this current flow in the proper direction in order to warm or cool
according to the instructions coming from the microprocessor through peltier unit. The optical block is
equipped with a dissipator and a fan in order to dissipate the heat coming from the cuvette holder.
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There is a temperature sensor that measures the temperature in the cuvette holder and the signal is sent to
the microprocessor through the amplifier. The thermostatic program is located in the microprocessor and,
according to the programmed temperature and to the current value, it switches the power control on,
warming or cooling as required.
The light source is an halogen lamp. The light goes first trough the optical lent and through the diaphragm
that limits the amount of light and directs the beam towards the flow cell. After the flow cell, the light goes
towards the interferential filter located in the filter wheel that is moved by a stepping motor and behind the
filter there is the photodetector plus preamplifier, protected by a cover, that convert the light into a electric
signal that will be used by the electronic circuits to perform the measurement.
magnet
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Preamplifier
board
Filter motor
Alignment sensor to
magnet
The filter motor home sensor is a HALL effect sensor, so it detects a magnetic
field generated by a magnet mounted inside the filter wheel.
The alignment of the sensor with magnet can be performed by losing the screw
that fix the magnet sensor to the filter box and move sliglty up/down the
sensor, till the magnet is properly detected.
The magnet is properly detected when the output between ground and the yellow
terminal of the 3 wires that exit form the sensor PCB goes to 0 to 0.5 V.
Filter wheel
Magnet for
filter wheel
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4. ELECTRONIC CIRCUIT
4.1 Block Diagram
PC group is not present in FULLY SMART
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Speaker
PC Peltier for
optical
(external in Lamp: group
Preamplifier Activity
board LED
FULLY SMART) ~ 11V termostating
Prehetaer
termostating Syringe Electrovalve Filter Optical
motor wheel group 12V DC
motor FAN (60x60)
Level
sensing
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N.B.
Mother Board, Hard
disk, Cd Rom and
5 4 Floppy disk are not
present in FULLY
SMART
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ID: 3
ID : 4
ID : 5
ID : 6
ID : 7
ID : 8
RX TX LED
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READING BOARD
COD. 110702
T40 C Power supply
TO BOARD
COD. 110703
PRE-
AMPLIPHIER
CONNECTOR
Board ID 1
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RED ON = Heating
GREEN ON = Cooling
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• Priming diluter
• Wash cuvette and needles
• Peristaltic pump auto - calibration
• Volume calibration.
The following calibrations needs to be performed on the instrument only as extraordinary service is required:
Explanation:
• Initialize: Basi alignment and homing. Will perform all the motors on the start right position.
• Prime diluter: will perform 3 cycle of diluter priming. Useful to fill up all the hydraulics, for checking
hydraulics, to remove air bubbles from syringe. Perform this at the first installation of analiser.
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• Wash cuvette: will perform an aspiration with peristaltic pump through cuvette. Can be used if the
peristaltic pump needle is dirty or blocked and the instrument do not have good aspiration from peristaltic
pump. Sodium hypoclorite (10% solution in water) can be a good washing solution.
• Volume calibration: only perform this kind of calibration if you are experiencing too high dead volume in
reagents bottle or if a washing cycle is not efficient (because of the too high residual volume left in the
washing well), or too high residual solution inside the reaction cuvette, or too high carry over.
• Photometer check: manual functioning of photometer.
• Pump calibration: to compensate the loose of the peristaltic pump rubber, an auto calibration of
peristaltic pump is recommended as occasional service operation. Good value should be inside: 0.8 –
3.5. Use this too decrease carry over.
• Service: by pressing this button you will access to service menu (extraordinary maintenance). This
operation needs password and is for authorized person only.
WARNING:
THIS MENU IS PASSWORD PROTECTED BECAUSE AN INCORRECT OPERATION AT
THIS LEVEL MAY DAMAGE SERIOUSLY THE INSTRUMENT. IF YOU DO NOT KNOW
WHAT YOU ARE DOING NEVER ENTER TO SERVICE MENU AND EXTRAORDINARY
MANTEINANCE.
5.2.1 Service
This is the maintenance to be done every time you disassemble the instrument for changing some of its
hardware parts.
When you click Utility button on the Main menu, you enter in Service.
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Execute menu:
• STOP button: reserved for internal use
• Execute buffer button: reserved for internal use
Temperature menu:
• Quick view to temperatures.
Action menu:
• Refresh: Is refreshing board version and temperature status in board menu and temp menu.
• Macro: Single command parametric execution.
• Reset board: reset all boards.
• Layout: Will give you access to layout and calibration menu (see later)
LAYOUT MENU:
• Reagent layout: reagent position centering.
• Sample tray: sample tray centering
• Reaction tray: reaction well centering
• Washer: washing well centering
TEST MENU:
• Volume: volume adjustment, do not use. Use Utility -> volume calibration instead (from main
nnnnnnnnnnnnnnnnmenu).
• Sensor: to test the status of the sensors.
• Temperatures: to test the status of the temperatures.
• Photometer: to check the photometer
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CALIBRATION:
• Pump: peristaltic calibration, do not use. Use Utility-> Pump calibration instead (from main menu).
• Diluter: needs non adjustment. Do not use.
SPECIAL:
Burn In: Performs randomic movement cycles.
The instrument will select the 1st reagent (the one that is marked “1” on the reagent tray). Adjust it to its
position using the steps arrow (left and right).
When reagent 1 is centered, press “Last” button.
The instrument will select the last reagent.
Center in the same way the last reagent.
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The instrument will select the 1st sample (the one that is marked “1” on the sample tray). Adjust it to its
position using the steps arrow (left and right) for horizontal arm.
Use also left and right for rotor centering if needed.
When sample 1 is centered, press “NEXT >” button.
Note that if you exit without saving you will loose your changes.
Click “cancel “ in every moment to exit.
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The instrument will select the 1st reaction well (the one that is marked “R1” on the reaction well tray). Adjust
it to its position using the steps arrow (left and right) for horizontal arm.
Use also left and right for rotor centering if needed.
When the position 1 is centered, press “NEXT >” button.
The instrument will calculated all the other values, and will select “R7” (second row).
Center it in the same way as done with “R1”.
When sample “R7” is centered, press “NEXT >” button.
Note that if you exit without saving you will loose your changes.
Click “cancel “ in every moment to exit.
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Note that if you exit without saving you will loose your changes.
Click “cancel “ in every moment to exit.
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The instrument has a special mode that lets you to know if the sensor are working properly or not.
Click “cancel “ in every moment to exit.
Washing solution is OK
Waste is full.
No Liquid
Liquid is detected.
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Open the instrument as explained in section 6.3. You have to work on Temperature board, on the left side of
the instrument, shown in the figure below.
Put the multimeter metal point in order to read the voltage between test – point TP2 (TEMP) (marked in
yellow in the figure below) and the ground marked GNDA (marked in red). Move the trimmer RV1 (marked in
orange) in order to read the voltage 3.95V to 4.0V. This will set temperature near to 37°C (+/- 0.5°C). If
needed more precision use temperature SOFTAWRE FINE TUNING.
FINE TUNING
Without opening the instrument, switch it on. The Main windows appears.
Click on Utility bottom
Now, on the Utility window, click on the Service button (“service” is password
required)
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Click on the button with the number 37° on the “Flow cell “ square
Put the thermocouple in the cuvette lodging. Take care that the thermocouple touches the bottom of the
lodging. Wait 15 minutes in order to reach the temperature homogeneously.
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NOTE:
Calibration is no needed for Preheater and reaction well rotor (first incubation). This temperatures
are digitally controlled by microprocessor.
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GAIN ADJUSTMENT
Without opening the instrument, switch it on. The Main windows appears.
Click on Utility bottom and then on the Service button (“service” is password required)
Click on the Layout button and than click on Test next to Photometer.
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In the section “Filter”, select “340nm” and verify that the value on T1 window is between 1600 and 8000. If
the value is out of range, you have to adjust the trimmer RV2 that is on the optical group. In order to work on
the trimmer RV2, you have to open the instrument as explained in section 6.3. The RV2 trimmer is marked in
red in the figure below.
T2 is not important and not used by this instrument.
PRE
AMPLIFIER
OFFSET
PRE
AMPLIFIER
GAIN
Adjust the GAIN trimmer in order to obtain that all the value on all the wavelengths are inside the range 1600
and 8000.
IMPORTANT:
The trimmer RV2 acts as a general GAIN. Increasing or decreasing the GAIN will affect all the wavelength
transmittance value (T1).
T1 is a parameter that is related to the opposite of the filter transmittance.
The BIGGER is the value of T1, the LOWER is the filter transmittance.
The T1 value is printed each time you perform a test with the instrument as INITIAL BASELINE.
The instrument has autodiagnosys on startup to check if this value exceeds the maximum treshold allowed.
If so the instrument will give you a warning:
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OFFSET ADJUSTMENT
Offset adjustment is achivied by setting the photodiode dark current to 0 when there is not light in to the
photodetector surface. Note that this calibration is stable and needs to be required rarely or after major
revision and upgrade of the instrument. Recommended a revision every 2/3 years.
To make this calibration, the following procedure should be applied:
OFFSET
5. Adjust the trimmer RV1(OFFSET) of the preamplifier board
till the value between 0 and + 1.0 mV is reached.
GAIN
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6. MAINTENANCE
Be careful in the case you open the instrument for operation because of possible electric shocks
1. Disconnect the bottle’s connectors from the vertical wall (C1 and C2 in fig).
2. Disconnect the suction and dispensing flexible tubes. In order to avoid the leakage, you have to follow
these steps
You have to rotate the peristaltic pump by hand anti clockwise in order to make the tube empty.
Take off the tubes in this order: n°1, n°2
Move the arm in order to have the needles above the rotor gear plate, and then, take off the tube n°3.
Take off the tube n°4 from the connection.
WARNING: the calibration of the reaction wells and needles centering is lost when the transfer
arm is disassembled, thus is necessary to re-calibrate both afterwards.
• Disconnect the suction and dispensing flexible tubes as shown in section 6.1.
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A A B
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The casing consists in 2 principal parts: the upper part that contains the
round plexiglas protection and the base, on which there is the reagent plate.
This two parts are fixed together by 6 screws and they are fixed to the
aluminum base by two screw on the front side and two on the back side.
1. Take off the arm case, unscrewing the 3 screws on the top and
detaching the connectors that link the two led with the main board
2. Disconnect the suction and dispensing flexible tubes as shown in
section 6.1.
3. Remove two screws on the front side and two on the back side of the
instrument
4. Lift the little case cover that is under the arm (in picture below, it is
indicated with the big black arrow).
5. Detach the connector that links the plate led with the main board that is on the left back side of the rotor
gear group.
6. Take care that there is a connector coming from the display and the ground wire that are so longer
(1,5m) but you have to take the case near the instrument.
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7. Reverse this steps to reassemble, taking into account to perform the calibration of the reaction well and
needle centering. Take care not to crunch the display connector and the ground wire under the case.
1. Remove the nuts and the washers that fix the dissipator to the instrument.
2. Remove the dissipator.
3. Remove the screws fixing the main board to the base.
4. Remove the faulty board and reverse the process to reassemble with the new one.
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7. Remove the 4 screws that fix the optical group to the base, marked with a circle in the picture.
8. Reverse this steps to reassemble.
WARNING! Proceed very carefully when handling the filter wheel in order to avoid to scratch or soil
the filters.
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Take off the case taking care not to move the transfer arm group. Now you can fasten the screws.
6. Attach the connectors to the main board as shown in the following pictures.
Attach here the connector coming
from the new motor
This is the board on the vertical wall,
on the right side of the transfer arm
group
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To detect if the reaction well warmer is working properly, the metal should reach the target temperature of
40°C, this to ensure 37°C in the reaction well itself.
The resistance between the connector to the resistance that HEATS the plate is approximately 4.5 Ohm.
4.5 Ohm
Safe themostate
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6.24 Lubrication
In order to have a fluently movement of the arm (vertical and horizontal), of the rotor gear and of the diluter,
you have to lubricate the following components one time in a year. If the instrument works in a dusty
ambient, you have to lubricate two times in a year.
• Up/Down movement: you have to lubricate the belt using grease and the two columns using oil
• Rotation arm movement: you have to lubricate the belt using grease
• Rotor gear movement: you have to lubricate the belt using grease
• Diluter: you have to lubricate the belt and the revolving screw using grease
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Refer to this picture for the highness on mountng the needles correclty.
Note that the aspiration needle (connected to flow cell) is edge cutted and should be mounted 0.5 to 1 mm
lower than the sampling (or preparation) needle (connected to the syringe). This will prevent accidentally
obstruction of the sampling needle.
METALLIC PLATE
and ARM COVER
NEEDLE SUPPORT
(Derlin or Teflon)
ASPIRATING
NEEDLE
(to flow cell)
SAMPLING NEEDLE
(to sirynge)
55 mm
0.5 mm
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In order to get an optimal operation of this instrument, it is necessary to follow some minimal maintenance
rules.
1. Never use detergents or abrasive products for cleaning the outside of the instrument. Use only a cloth
with water and neutral soap.
2. Avoid the penetration of liquid into the inner part of the instrument. If liquid is spilled into the instrument,
clean it with damp paper or cloth.
3. Put the display in the down position when not in use in order to avoid to hit it.
4. Close the round plexiglas protection when not in use in order to avoid dusty.
7.1 Cleaning of the optical components (lamp, lens, filters and photodiode)
Recommended material:
non abrasive, lint free paper
alcohol + ether (50/50) solution
cotton ear picks
lens cleaner (blower type)
For a proper manipulation of the optical components, the following general precautions should be taken
into account:
→ The working area should be clean and in order
→ As the components are fragile, they should be treated carefully; a fall could result in breakage
→ Avoid touching the operative surfaces with the fingers. Lenses, filters and photodiode should be held
by their sides and the lamp by its connecting terminals.
→ To clean the components, first undust with the rubber bulb to avoid the scratches caused by small
particles on the surface when rubbing with the paper.
→ In case of persistent or greasy dirt, slightly rub with a paper moisten with the alcohol/ether solution
and then with a dry paper. Sometimes, when cleaning the filters or the photodiode window, the use
of the cotton hear picks may be helpful together with the paper to clean the most delicate parts.
→ Once the cleaning is finished it is convenient to go over with the rubber bulb in order to remove any
residual paper or cotton lint left on the surface.
→ When assembling or disassembling any component take care to use the corresponding tools and
follow the written procedures, since they have been devised to avoid manipulation problems.
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Insert the syringe in the tube so that small internal tube is inside the syringe and large external tube is
outside of the syringe:
Place a reagent bottle filled with water in a convenient position and rotate the arm until it reaches the bottle.
Move the arm down inside the bottle until the green led turns on. Push and pull the piston of the syringe
slowly several times: this will clean aspiration circuit.
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ROTOR MECHANISM
1. Verify the belt tension. By rotating the driving pulley, its movement be fully transmitted to
the pulley axle.
2. Verify the centering of the reaction wells in the heating channel.
PRE-HEATER
1. Its maintenance consists only on checking the proper status of the pre-warming channel
and the tray.
ARM MECHANISM
1. Verify the tension of the belt in charge of the horizontal and vertical movements.
2. Lubricate the displacer guides (use oil S.A.E.:30 or similar)
DILUTER MECHANISM
1. Verify the tension of the driving belt.
2. Clean and lubricate the revolving screw (use oil S.A.E.:30 or similar)
OPTICAL SYSTEM
1. Clean the optical components.
2. Clean the filters.
3. Clean the lens.
4. Clean the photodiode.
5. Wash the flow-thru cuvette.
6. Calibrate the photometric system.
PIPETTING SYSTEM
1. Check the water-tightness of the syringe piston. Verify that there is no leakage or
formation of micro bubbles. Substitute in that case.
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SIPPING SYSTEM
1. Change the teflon tubing.
2. Change the peristaltic tubing.
3. Check the waste tubing. Change it in case of cracking or obstruction.
APPENDIX 1
General characteristics
Processing capacity: up to 54 positions (including samples, calibrators and controls) per tray in a work plan.
Incubation 1: 21 to 9999 s
Incubation 2: 0 to 180 s
Unlimited replicates for blanks, calibrators and samples
Calibration storing
Patient data (name, age, sex, etc. ) files – demography data base
QC
Sample tray
Sample cup capacity: 1.2 ml maximum
Tray capacity: 54 cups for samples, calibrators and controls
Reagent tray
Tray capacity: 20 reagent bottles of about 45 ml
Reaction wells
Reservoirs
Wash bottle: 0.5 l
Waste bottle: 0.5 l
There is also the possibility to connect to external waste tank.
Programming
Tests: unlimited
Profiles: Up to 9 with an unlimited number of tests
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Calibrators
Controls
Filters
Reagents
Analysis modes
End point: 1 or 2 reagents
Differential
Fixed time
Kinetic
Multi Standard
Kinetic analysis
Absorbance measurements during the programmed interval
Linearity evaluation
Use of factor or calibrator
Calibration types
Factor
Single calibrator: for one test (specific) or common to several test (multiple)
Calibration curve
Calibration curve
Up to 8 standards
Axes: Linear and Logarithmic
Calculation functions: Spline, Linear Regression, Square Regression, Polygonal
Quality Control
Analytical limits Control: Blank, Linearity, Factor
Up to 3 control materials per test
Levey- Jennings charts/shewart
Temperature control
3 thermostated areas
Reagent pre-warmed in the transfer arm (+/-1°C)
Reaction mixture thermostated in the reaction wells to 37°C ± 2°C
Reaction mixture thermostated in the flow cuvette to 37°C ± 0.2°C
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Optical system
Transfer system
The personal computer inside the instrument has the following requirements:
Intel Processor
128 Mbytes RAM
Hard disk capacity> 20 GBytes
Operating system: Windows™
Drive for 3.5” 1.44 Mbytes disks
Cd-rom
Built-in network adapter
Output: USB port
Built in thermal printer, 120 mm (N.B. the thermal printer is present also in FULLY SMART)
TFT 12”Display, 800x600
Physical dimensions
FULLY
680 (wide) x 720 (large) x 750 (high) mm open display /
680 (wide) x 720 (large) x 560 (high) mm closed display
Weight: 55 kg
FULLY SMART
680 (wide) x 720 (large) x 560 (high) mm
Weight: 55 kg
Electrical requirements
Assistance to users
Automatic selection of the calibrators and controls required for a work plan
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Graphics
Calibration and Kinetic curves
Quality Control (Levey-Jennings)
APPENDIX 2
CODE DESCRIPTION
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APPENDIX 3
TUBING
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APPENDIX 4
TROUBLESHOOTING
SECTION A:
Operational and manteinance error (no need of opening the instrument cover)
Aspiration needle
45 mm
Dispensing needle
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Pump calibration value > 1.6 - Aspiration needle is obstructed (clean necessary from top
to bottom)
- Tube disconnected (check)
Too long time depleting washing cup > 20 sec. - Aspiration needle obstructed
- Volume calibration required
Air bubbles in to the cuvette - Flow cell is dirty (need washing with deproteinizer agent)
- Check tube size (110 to 125) and air gap value
- Peristaltic pump calibration needed
- Aspiration needle obstructed
Volume calibration - R1 not present (put R1 in the carousel)
Error: R1 not present or air into the dispensing circuit - Air inside dispensing circuit (make diluiter prime)
- Dispening needle obstructed (clean from top to bottom)
Prime diluiter - Perform volume calibration
Needles bump into the washing well - Make diluiter prime without washing cup, then put again
the washing cup and make volume calibration
High CV (low precision / repetibility) - Dispening needle obstructed (clean from top to bottom)
Diluiter drift observed for same sample - Use washing solution (perchloric acid 50% diluited) or
pepsine based solution for clean dispensing needle (use
special “clean dispening needle” program, in MAIN-
>utility)
Linearity test out of 3% max error - Adjust offset of preamplifier board through “Dark current
setting procedure”
- Check for obstruction in the needles
Too much residual solution IN ALL reaction well - Volume calibration is needed to adjust minimum volume
Too much residual solution IN SOME reaction well - Aspiration needle is obstructed. Need to be cleaned.
First sample underestimation in kinetic test (GOT, GPT) - Reagent is too cold (wait more before performing test)
- The cuvette is cold due to the previous washing: increase
st
the 1 incubation time
- Washing solution is too cold?
Leakage from ASPIRATION needle. - Check the connection of (B) tube with flow cell and
aspiration needle
- Check the peristaltic pump rubber grey tube
- Check the connection of (A) tube
- Check integrity of tube (A) and (B)
Leakage from DISPENSING needle. - Check the connection of (C) tube with (D) tube. Screw the
joining to seal it.
- Check the tube (D) connection to electrovalve. Replace
the O-ring if necessary.
- No need to thight excessive! O-Ring is present. Excessive
strenght will damage permanently the Electrovalve.
- Check integrity of tube (C) and (D)
No aspiration from WASH BOTTLE - Check the O-Ring presence and integrity on the left side
of electrovalve (E-tube)
- Check the connection of E tube
The probe is not connected properly
The probe does not aspirate sample and/or reagent If the module works correctly, check if there are problems in
the tubing and if there is enough washing solution in the
container.
Check the diluter valve
The probe does not dispense the solution into the sampling Check if there is enough wash solution in the container
well Check all the tubing.
Check the sampling probe. If it is dirty, clean it. If there are
The test using a 3 µl sample size is not reproducible some scratches on it, change it.
Check the flow cell; clean it if it is necessary.
Check if the aspiration probe may be dirty or blocked.
Bad aspiration into the flow cell. Check the tubing.
Check the movement of the aspiration arm.
Check if the probe is positioned correctly
Check the tubing from the well to the valve.
The peristaltic pump works correctly but the washing well is Check the valve.
not emptied. The silicon tube inside the peristaltic pump may be damaged
or badly set.
Check the tubing of the peristaltic pump.
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SECTION B:
Hardware error (need of opening the instrument cover and full board access)
Error Solution
- Check the connector of the sample rotor motor
Arm bump due to rotor misaligment and home sensor to the sample rotor board (ID
5).
Error msg - Check for false or bad contact of the connectors
HOME ERROR: ID 6 to the motor and to the home sensor. Plug the
HOME ERROR: ID 5 connector properly and check the continuity of
NEEDLE IMPACT ERROR: ID 6 the wiring with a “beeping” multimeter.
- Clean the home sensor by blowing pressured air
from the dust.
- The home sensor is not working properly and
causing spike. Change the home sensor board
(Generally this cause wide misaligment).
- Lubricate the belt and the gear of the motor.
- Check the dissipator of the board that is
assembled properly.
- Cuvette friction: switch of the instrument and
check that the sample rotor wheel is running
without friction (some cuvette are causing
excessive friction in to the rotor): use cuvette
supplied from BSI.
- Replace the motor control board (ID 5).
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Arm bump due to horizonatal arm misaligment - Switch off the instrument and check if tha arm
is running free without friction when moving it
Error msg by hand.
HOME ERROR: ID 6 - Check the connector of the horizontal arm home
NEEDLE IMPACT ERROR: ID 6 sensor to the horizontal arm motor board (ID 4).
- Check for false or bad contact of the connectors
to the motor and to the home sensor. Plug the
connector properly and check the continuity of
the wiring with a “beeping” multimeter.
- Clean the home sensor by blowing pressured air
from the dust.
- The home sensor is not working properly and
causing spike. Change the home sensor board
(Generally this cause wide misaligment).
- Lubricate the belt and the gear of the motor.
- Check the dissipator of the board that is
assembled properly.
- Replace the motor control board (ID 4).
Vertical movement of the arm malfunctioning - Switch off the instrument and check if the
vertical movement is running free without
Error msg friction when moving it by hand, without loosing
HOME ERROR: ID 6 position.
NEEDLE IMPACT ERROR: ID 6 - Check for the belt assembly (there is a block
that fix the belt to the arm for motion
transmission, fixed with screws. Check if this
screws are loose).
- Check for false or bad contact of the connectors
to the motor and to the home sensor. Plug the
connector properly and check the continuity of
the wiring with a “beeping” multimeter.
- Clean the home sensor by blowing pressured air
from the dust.
- The home sensor is not working properly and
causing spike. Change the home sensor board
(Generally this cause wide misaligment).
- Lubricate the belt and the gear of the motor.
- Check the dissipator of the board that is
assembled properly.
- Replace the motor control board (ID 6).
Encoder error - Switch off the instrument and check if the
Error msg horizontal movement of the amr is running free
Encoder error: ID 4 without friction when moving it by hand, without
loosing position.
- Check for false or bad contact of the connectors
to the motor and to the encoder board. Plug the
connector properly and check the continuity of
the wiring with a “beeping” multimeter.
- Check if the encoder board leds are active when
moving the horizontal arm by hand (Be sure that
the instrument is reset or not initialized!,
otherwise you can damage the horizontal arm if
it stay in tension).
- The encoder board needs to be replaced if the
activity leds are not blinking when moving by
hand the arm in horizontal way.
- Replace the motor control board (ID 4).
Timeout error on finding home - The home sensor is not connected or not working.
Error msg Check connections.
TIMEOUT ON FINDING HOME. ID (all) - The motor is not moving: check motor connection
to its moving board.
- Check if the flag of the home is correclty
entering into the home sensor.
- Check the continutiy of the home sensor with the
motor board.
Diluiter and syringe error - Check SW version: install latest release.
Error msg - Check if no cable are interfering with the motor
HOME ERROR: ID 3 or the flag that fit into the home sensor.
TIMEOUT ON FINDING HOME. ID 3
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Digital output.
Connected to board number 4.
Code number: 110700
Rules:
The encoder board works properly when turning the horizontal arm by hands the signal changes form 0 to
5V continuosly approximately every 10 steps.
The 3 LEDs on the encoder board must change their state when changing the positioning of horizontal arm.
Example:
Arm position sensor : Output = 5V
Different arm position : Output = 0V
2 1
FILTER MOTOR CONNECTOR:
4 MOTOR PHASES
Gnd Gnd
+5V +5V
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Digital output.
Connected to BOARD number 7.
Code number: 110700
Rules:
The sensor board works properly when turning the filter wheel by hands there is a point where the output
goes low (0V). This is the home position for the filter wheel.
Example:
Magnet is far from sensor : Output = 5V
Magnet is on the sensor : Output = 0V
2 1
FILTER MOTOR CONNECTOR:
4 MOTOR PHASES
Gnd
+5V
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Analouge output.
BOARD number 2.
Code number: 110703
Rules:
The sensor board works properly if output is related to temperature with the following equation:
Temperature = Output (mV)/10
Example:
Output = 235 mV means temperature detected by sensor board is 23.5 °C.
+8V
Sensor board
Gnd Output
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Digital output.
Connected to BOARD number 4, 5, 6, 3.
Code number: 110700
Rules:
The sensor board works properly when moving the motor by hands when the optical sensor is obstructed
the output goes low (0V). This is the home position for the motor.
You can also use a paper to obstruct the path of the optic sensor to diagnostic the sensor.
Example:
Optic obstructed : Output = 5V
Optic free : Output = 0.5V
Sensor board
2 1
MOTOR CONNECTOR:
4 MOTOR PHASES
Gnd
+5V
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-----------------------------
Computer info
-----------------------------
Family : 6
Model : 6
PHASE 1:
Speed : 1469 MHz
Information regarding current computer and operative
OS : Microsoft Windows 98 SE
Total memory : 128 MB system inside the instrument.
IP Address: : 10.0.0.1
Total disk space: 38160 MB
Free disk space : 31398 MB
-----------------------------
Initializing diagnostic.
2003-10-17 16:10:52 :Resetting hardware :OK!
2003-10-17 16:10:54 :Checking hardware :
Board number 1 version: 1.04
Board number 2 version: 3.00
Board number 3 version: 1.13 (PHASE 2)
Board number 4 version: 1.13
Board number 5 version: 1.13
Board number 6 version: 1.13
Board number 7 version: 1.13
Board number 8 version: 1.13
OK!
2003-10-17 16:11:06 :Checking tank level :OK! (PHASE 3)
2003-10-17 16:11:08 :Initializing motors :OK! (PHASE 4)
2003-10-17 16:11:34 :Checking temperature sensors :OK! (PHASE 5)
PCB : actual= 29.0 Set=24.0
Incubat: actual= 39.6 Set=37.0
Rotor : actual= 23.7
Preheat: actual= 34.8
2003-10-17 16:11:36 :Checking filter wheel : (PHASE 6)
Checking position 2 / 8: ...OK!
Checking position 3 / 8: ...OK!
Checking position 4 / 8: ...OK!
Checking position 5 / 8: ...OK!
Checking position 6 / 8: ...OK!
Checking position 7 / 8: ...OK!
Checking position 8 / 8: ...OK!
OK!
2003-10-17 16:12:05 :Now priming diluiter....OK! (PHASE 7)
2003-10-17 16:12:42 :Testing level sensors....OK! (PHASE 8)
Start volume: 3106 uL
2003-10-17 16:12:44 :Aspirating in flow cell....OK!
2003-10-17 16:12:55 :Testing level sensors....OK!
End volume: 1362 uL
Estimated error: 12 % (PHASE 9)
Pump calibration value: 1.506900
2003-10-17 16:12:58 :
------------------------------
Energy levels:
Filter 1 --> 26823 (Old=5326) *WARNING: Energy level under LOW LIMIT*
Filter 2 --> 14009 (Old=4180) *WARNING: Energy level under LOW LIMIT*
Filter 3 --> 7518 (Old=274)
Filter 4 --> 16406 (Old=6070) *WARNING: Energy level under LOW LIMIT* (PHASE 10)
Filter 5 --> 16036 (Old=5998) *WARNING: Energy level under LOW LIMIT* Filter energy values
Filter 6 --> 13984 (Old=5105) *WARNING: Energy level under LOW LIMIT*
Filter 7 --> 15132 (Old=4319) *WARNING: Energy level under LOW LIMIT*
------------------------------
2003-10-17 16:13:15 :Today calibration: 14620 (PHASE 11)
Factory calibration: 11469
2003-10-17 16:13:19 :Compensation values: 14640
Factory calibration: 11469 (PHASE 12)
6 warning(s)
SUCCESS!
MEANINGS
The instrument may fail in one of this phase of autodiagnosys. This is useful to understand the problems.
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PHASE 2: Generally timeout error. A Board is not connected or (if connected) the board is not working properly. Identification
number may be corrupted.
PHASE 3: Tank sensor not working properly or the washing tank sensor is disconnected. Check if the sensor are working with
a multimeter (valid state are 0 or 5 V). If the sensor are working properly, the problem is inside the board number 8
(the board to which is connected tanks sensor)
PHASE 4: The motor cannot initialize. Generally home error. Cause may be motor disconnected (in this case the motor is not
moving) or the home sensor not working properly (Timeout on finding home ). Refer to the ID of the defective board,
and check its connection to the motor and if the home sensor is working properly or not (see procedure above).
PHASE 5: Temperatures:
PCB : actual= 29.0 Set=24.0 <- Internal on the instrument
Incubat: actual= 39.6 Set=37.0 <-Target is 37°C
Rotor : actual= 23.7 <-Target is generally 40°C
Preheat: actual= 34.8 <-Target is around 50°C
If there is a reading error, may be a cable disconnect or a temperature sensor not working properly.
Check with procedure relative.
PHASE 6: Filter positioning check. If error in this phase, there is too much distance between the filter wheel magnet and sensor
in its housing. You should disassesmble the filter wheel and assemble it properly. You can also align the hall sensor.
PHASE 7: If error in this phase you may need to perform a volume calibration, or you have the dispensing needle (the one on
the left, connected with the syringe) obstructed. Use the cleaning needle to clean it.
PHASE 8: Error on level sensor. Touch the needles with the finger and see if the LED GREEN is on.ì (should be ON when
inside water, OFF when outside). If problem check connection of predosifing board with board number 6.
PHASE 9: If error or warning in this phase, recalibrate peristaltic pump through utility menu.
Good calibration value are between 0.8 to 1.8.
If value is bigger, you probably have the aspiration needle obstructed. Use the cleaning needle to clean it.
PHASE 10: If error or warning in this phase are due to filter energy level.
Energy too high. Decrease the gain of the preamplifier board (see page 34).
Energy too low. Increase the gain of the preamplifier board (see page 34).
THE GAIN WILL ACT IN ALL THE FILTER, DECREASING/INCREASING ALL FILTERS ENERGY VALUES.
Pay attention, all the filter must be inside the range, without warnings printed out. If you have to act on a single filter,
please disassemble the filter wheel and add or remove some gelatine (type 5, the lighter, is corrresponding to
1000).
PHASE 11: Calibration value. Check if is inside (9000 to 18000). If outside, there may be problem on preamplifier board or
reading board.
PHASE 12: Compensation values. Factory calibration.
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Unless specified explicitily during the installation or upgrade process, some file maybe backupped during software installation and
upgrade, in order to preserve user job and settings.
C:\instrument
BACKUP
In case you need to backup the software, the simplest things to do is to backup the whole c:\instrument folder. This ensure that no data
is loosed and that the periodic backup is fulfilled completely.
You can copy the c:\instrument folder into a memory stick via USB port to achivie this task.
FILE EXPLANATION
In case a software upgrade is done, some of the setting can be preserved without having to reinstall or edit from the beginning.
This file are inside the c:\instrument folder.
The meaning of the files is explained.
Setting Folder User setting and tables for the workplan. To be backupped and restored.
Tables Folder Hardware tables folder. No necessary to backup, automatically restored.
Utility Folder Not used
Rack.dat File Configuration with the position of the reagents (GLUC at 1, GOT at 2, ...)
Cqdata.mdb File Database containing the archive of the Quality Control data
DbDemography.mdb File Database of the patient demograpy information
Method.mdb File Database of the reagents and methods and quality control and calibrator information
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