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Contents

CONTENTS

S.No. Particulars Page No.


1 Introduction 1-8

2 Objectives 9-10

3 Review of literature 11-27

4 Materials & Methods 28-61

5 Results 62-71

6 Discussion 72
7 Summary 73-74
8 References 75-8
LIST OF FIGURES

Page
S. No. Name of the Figures
No.
1 Growth and spreading of cancer. 3
2. Soft tissue sarcoma. 5
Global Maps Present (A) the 4‐Tier Human Development Index and (B) 20
Areas of the World. The sizes of the respective populations are included in the
3. legend. Source: United Nations Procurement Division/United Nations
14
Development Program.
Pie Charts Present the Distribution of Cases and Deaths for the 10 Most
Common Cancers in 2018 for (A) Both Sexes, (B) Males, and (C) Females. For
4.(a-c) each sex, the area of the pie chart reflects the proportion of the total number of 15-16
cases or deaths; nonmelanoma skin cancers are included in the “other” category.
Source: GLOBOCAN 2018.
5. Ovarian cancer incidence exhibits wide geographic variation. 24
6. CO₂ incubator 29
7. Inverted microscope 30
8. Laminar air hood/ Bio-safety cabinet 32
9. Centrifuge 33
10. Plate shaker 34
11. Plate reader 34
12. Chemidoc 35
13. Microscopic Image of Breast Cancer Cell Lines. 43
Cell counting chamber and graphical representation of grid present in the
14. chamber observed under microscope.
47
15. Binding of Annexin V/PI Staining 55
16. Different stages of cells during Annexin V/PI Binding. 56
Figure shows how western blotting separates the sample in gel, transfer to the
17. membrane, staining from antibody and visualization.
61
Percentage inhibition of all compounds in prostate cancer and ovarian cancer
18 cell lines.
64

19 Percentage inhibition of all compounds in breast cancer cell line 65


20 Effect of CDRI-2 on MDA-MB-231 cells in 24 hrs. 68
21 Effect of CDRI-2 on MDA-MB-231 in 48 hrs. 69
22 effect of CDRI-2 on MCF-7 cell in 48 hrs. 69
23 Effect of CDRI-2 on MCF-7 in 72 hrs 70
24 Effect of CDRI-2 on Autophagic Marker LC-3 II in MCF-7. 71
LIST OF TABLES
S. No. Name of the table Page no.
1 Established and probable risk factors for breast cancer 18-19
Rough guideline of surface area and its recommended cell seeding
2 40
density, media, and trypsin volume and expected cell yield
Percentage inhibition in cell viability of all compounds in breast
3 63
cancer cell lines
4 Percentageinhibition of all compounds in breast cancer cell lines 64-65

5 Percentageinhibition of all compounds in different cancer cell lines 66

6 IC50 of Different CDRI Compounds 68

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