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International Journal of Chemical Studies 2019; 7(3): 1632-1637

P-ISSN: 2349–8528
E-ISSN: 2321–4902
IJCS 2019; 7(3): 1632-1637 Post-harvest processing of custard apple (Annona
© 2019 IJCS
Received: 19-03-2019
squamosa L.): A review
Accepted: 21-04-2019

Charul Jain, Champawat PS, Mudgal VD, Madhu B and Jain SK


Charul Jain
M. Tech Student, Department of
Processing and Food engineering, Abstract
CTAE, Maharana Pratap Custard apple being highly perishable fruit, is highly susceptible to spoilage, softens very rapidly during
University of Agriculture and ripening, and becomes squashy and not easy to consume fresh. The fruit has a short shelf-life due to high
Technology, Udaipur, respiration and ethylene production. Hence there is greater need to preserve the custard apple fruits and
Rajasthan, India to process into suitable products viz., juice, jam, ice cream, milkshake etc. to minimize the post-harvest
losses which further helps in value addition and in generation of a good income for a needy farmer.
Champawat PS Therefore we attempt here to review some important aspects such as the effect of packaging material and
Professor and Head, Department
chemical treatments on the storage and shelf life of fruit pulp including the use of modified and
of Processing and Food
engineering, CTAE, Maharana
controlled atmosphere, processing of stored pulp into various value-added products.
Pratap University of Agriculture
Keywords: Custard apple, storage, value addition, packaging, post-harvest processing
and Technology, Udaipur,
Rajasthan, India
Introduction
Mudgal VD Custard apple (Annona squamosa L.), is an important dry land fruit which belongs to the genus
Professor and PI, AICRP Annona of the family Annonaceae, which is indigenous to tropical America and found in
PHET, Department of tropical, subtropical and arid zones of the world. Out of 100 species of Annona, only 5 species,
Processing and Food engineering,
namely the custard apple, cherimoya, soursop, bullock’s heart and atemoya are of commercial
CTAE, Maharana Pratap
University of Agriculture and importance. This fruit is sometimes considered as poor man’s rich food in the arid zones of the
Technology, Udaipur, country. In India, production of custard apple is 298.01 thousand tonnes (http://apeda.in) [1].
Rajasthan, India Custard apple (Annona Squamosa L.) fruit grows well throughout the plains of India at
elevations not exceeding 4,000 ft. It prefers tropical climate. Tree shows yellow trumpet-
Madhu B shaped flowers that emit a pleasant sweet smell, with only a small number of flowers setting
Ph.D. Scholar, Department of
Processing and Food engineering, fruit. The fruits are variable in shape with the outer aspect being covered in rounded knobs,
CTAE, Maharana Pratap with the inside containing a custard like flesh. Taxonomy of fruit is given in Table 1.
University of Agriculture and
Technology, Udaipur, Table 1: Taxonomy of custard apple
Rajasthan, India
Kingdom Plantae
Jain SK Family Annonaceae
Professor, Department of species A. squamosa
Processing and Food engineering, order Magnoliales
CTAE, Maharana Pratap Genus Annona
University of Agriculture and
Technology, Udaipur,
Rajasthan, India
Custard apple has many medicinal properties like pain relief and wound healing. It is also used
as an antioxidant, anti-diabetics, hepatoprotective, cytotoxic activity, gene-toxicity, antitumor
activity and used as antilice agent (Pandey and Barve, 2011) [2]. This fruit also has the ability
to lower blood glucose levels. The anti-bacterial properties of custard apple can kill strains like
E.coli, but may also benefit acne (https://india.curejoy.com/content/health-benefits-of-custard-
apple/) [3].
Storage of the fresh fruits of (A. squamosa) and its pulp, has limitations, since it is perishable,
and cold storage is not promising because of the development of unattractive brown colour
which decreases the market value. The shelf life of fruits can be improved by slowing down
Correspondence the biochemical processes during storage by various treatments and by freeze-drying of pulp.
Charul Jain
M. Tech Student, Department of
But freeze drying of pulp appears commercially unavailable because of high costs. Therefore
Processing and Food engineering, there is a need to develop some cost-effective methods of preservation.
CTAE, Maharana Pratap
University of Agriculture and Fruit composition
Technology, Udaipur, The custard apple fruit is mostly used as a dessert fruit because of its delicious taste and
Rajasthan, India nutritive value. This fruit contains moisture (70.5%), carbohydrates (23.5%), proteins (1.6%),
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International Journal of Chemical Studies

fat (0.4%), mineral matter (0.9%), iron (1.0%), calcium storage of fruit decreased in consumer acceptability of fruit
(0.2%), phosphorus (0.04%) and energy value 104 Kcal/100g (Pawar and Hashmi, 2010) [11].
of the edible portion (Gopalan et al., 2004) [4]. Custard apple
fruit has TSS of 26.40B, pH 5.5 and contains 0.5% tannins. Factors influencing storability
The edible portion of the fruit varies from 28-37% of the total Effect of temperature
fruit weight and seeds correspond to 23-40% (Kumar et al., Ripening of custard apple fruits occur at temperatures
2014) [5]. Beerh et al., (1983) [6] studied the chemical between 15 and 30 °C, although the fruits were susceptible to
composition of custard apple pulp of 7 different varieties and fungal attack at temperatures above 25 °C and storage
concluded that all varieties were a poor source of vitamin C, temperatures below 15 °C caused chilling-injuries. Ripening
pectin and protein. The total soluble solids varied from 20.6- delayed by the addition of carbon dioxide or removal of
28°brix, total sugars from 14.9-21.6%. Nutritional oxygen and ethylene had no apparent effect on ripening.
composition of custard apple is given in Table 2 (Leung and Proposed conditions for storing custard apple are:
Flores, 1961; Janick and Paull, 2006). temperatures between 15 and 20 °C, low oxygen and ethylene
tensions coupled with 10% carbon dioxide and a relative
Table 2: Nutritional composition of custard apple (Food value per humidity of 85–90% in the storage atmosphere (Broughton
100g of fresh edible portion) and Guat, 1979) [12]. Chemical changes in sugar apples stored
S. No. Particulars Quantity at 26, 21 and 16 °C with 85-90% relative humidity were
1 Moisture 70-80% followed to determine the optimum storage and ripening
2 Sugar 18.0-24.0% conditions. The fruit weight loss was higher at higher storage
3 Fibre 0.05-4.5% temperatures than the fruits stored at lower storage
4 Protein 1.0-2.0% temperatures. Results showed that higher the temperature
5 Ash 0.4-0.8% was, the more rapid the increase in glucose, fructose and
6 Lipid 0.3-0.6% sucrose concentration. The starch of the fruits gradually
7 Energy 95-110(kcal) decreased as the storage period proceeded (Chen W, 1999)
8 Minerals 332.4mg [13]
. The effect of storage temperature on ripening, shelf life
9 Ascorbic Acid 10-300mg and chemical composition of custard apple fruits stored at 10,
10 Niacin 0.8mg 15, 20 and 25 °C was studied. The storage temperature
11 Vitamin E 0.6mg between 15 and 20 °C was found to be safe, with maximum
12 Vitamin B6 0-0.2mg
shelf life at 15 °C. The colour of the pulp, texture, taste and
13 Vitamin A 0-6IU
flavour of ripe fruits held at 25 and 20 °C were superior
followed by fruits stored at 15 °C (Prasanna et al., 2000) [14].
Post-harvest processing of custard apple
The custard apple pulp from mature ripe fruits was subjected
Post-harvest losses are defined as any measurable qualitative
to treatments such as frozen and stored (upto 12 months),
and quantitative loss of food along the supply chain, starting
heated to 55 °C (critical temperature) and 85 °C
from the time of harvest until its consumption or other end
(pasteurization temperature) for 20 min each, and was spray
uses (Hodges et al., 2011) [7]. The post-harvest losses of fruits
dried with skim and whole milk powders. The 12-month-
and vegetables are about 35-40%. This is due to infrastructure
stored frozen pulp was almost same as fresh pulp in the
and management limitations of a given food value chain. The
flavour spectrum. Heating fresh pulp at 55 and 85 °C, tended
management of higher crop production and its conservation in
to produce increased flavour spectrum, the compounds
quality and quantity is necessary to reduce post-harvest
relatively being more at 85 °C (Shashirekha, 2008) [15]. The
losses. Postharvest losses result not only in the loss of the
custard apple pulp stored at -4 and -18 °C temperature
actual crop, but also have an adverse impact on the
showed an increase in sensorial quality viz. Appearance,
environment, resources, labour needed to produce the crop
colour, flavour, texture, and overall acceptability and it can be
and livelihood of individuals involved in the production
stored up to 70 days was reported (Kamble and Soni, 2010)
process. The reduction in post-harvest losses will contribute [16]
. The effect of heat treatment on changes in sensorial
significantly to increasing the availability of nutritionally very
quality (browning and discolouration) was studied and results
important fruits.
showed that steam heating of custard apple pulp at 83 °C
temperature for 2 minutes leads to complete inhibition of PPO
Harvesting and Storage of Custard apple
activity while complete inhibition of PPO activity was also
Custard apple fruits should be harvested at the normal time or
observed at 82 °C for 5 minutes (Pawar et al., 2011) [17]. The
2-5 days earlier (when TSS around 10%) and stored at 25-33
effect of different storage temperature on the storability and
°C at 85-95% relative humidity. For immediate consumption,
postharvest quality attributes of custard apple was examined,
fruits should be harvested at total soluble solid content around
the postharvest custard apple were stored at 4, 15 or 20-28 °C
15-20% (Chen, 1999) [8]. Harvesting of fruits was done at
(room temperature) by measuring the change of weight loss
physiological maturity and stored at 26±2 °C at 60-70%
rate, total soluble solids (TSS), firmness and titratable acid
relative humidity for 4 days (Bolivar et al., 2009) [9]. When
(TA). The result showed that the shelf life of fruits were 4, 7,
fruits were harvested at maturity stage, a climacteric peak was
10 days with 22-28 °C (room temperature), 15 °C, 4 °C,
noticed in the second day of storage at ambient conditions and
temperatures respectively (Cheng et al., 2018) [18].
respiration peaks of 56.32 mg CO2/kgh and 27.42 mg
CO2/kgh respectively (Mallikarjuna et al., 2012) [10]. The
Effect of Chemical Treatment
custard apple fruit harvested at the commercial stage of
Custard apple pulp, when comes in contact with air, turns
maturity resulted in an increase in total soluble solids, total
pink due to activity of peroxidase enzyme and becomes bitter
sugar and acidity up to 4th day of storage, while on further
in taste when heated above 55 °C temperature, which renders
storage reduction in these parameters and organoleptic
preservation by heat treatment inapplicable. To preserve the
characteristics of fruit was observed. The maximum score was
pulp, it is necessary to add 1% of citric acid together with
observed in case of fruit stored till 4th day, while subsequent
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International Journal of Chemical Studies

0.1% of sodium benzoate, while the addition of 50–100 ppm. fruit ascorbic acid content was higher in fruits held in paper
of sulphur dioxide checks the pink discolouration due to bags and wheat straw, sugars were higher in fruits held in
enzymic activity (Sastry et al., 1961) [19]. Custard apple pulp polyethylene bags with sawdust (Chaudhry et al., 1985) [28].
treated with 100 ppm ascorbic acid as anti-browning agent The custard apple fruits packed in different packaging
and packed in 200 gauge polyethene was frozen at −25 °C in materials were stored at room temperature and zero energy
an alcohol bath and stored at −18 °C in a deep‐ freezer. The cool chamber. It was found that fruits could be stored up to 6
effect of three cryoprotectants (w/w), namely 0.5% propylene days under ambient storage, when wrapped with tissue papers
glycol (I), 0.5% glycerol (II) and 2% glycerol + 1% propylene and kept in cardboard boxes. However, the shelf life of
glycol + 10% glucose syrup + 10% maltodextrin (III), on the custard apple fruits was 9 days when wrapped with tissue
thawing time of pulp was studied for a period of 3 months. papers and kept in cardboard boxes when stored in zero
For comparison a control sample without any cryoprotectant energy cool chamber (Patil et al., 2011) [29]. The custard apple
was included. It was found that formulation (III) gave a fruits submitted to different packaging under refrigerated
product that was pourable even on frozen storage and also had storage. Fruits were unpacked (controlled), individually
the highest half‐ life of 75.33 days for ascorbic acid packed in polyvinyl chloride (PVC) films, or packed in
degradation as compared to 34.82 days for the controlled expanded polystyrene trays enveloped in PVC film, stored at
sample (Chikhalikar et al., 2000) [20]. Custard apple pulp 10 °C and tested at intervals of 0, 4, 8, 12 and 16 days. The
treated with sugar in the ratio of 1:1, 1:2 and 1:4 can be stored experiment showed that the use of packaging films
up to 90 days with minimum changes in physio-chemical significantly reduced weight loss, preserved firmness and
properties. Analysis showed that custard apple pulp treated colour of the fruit. The modified atmosphere allowed for a
with a sugar concentration of 1:4 shows best results (Kolekar, shelf-life up to 12 days at a temperature of 10 °C. The
2002)[21]. Custard apple fruits treated with 6% Waxol and 30 physiological weight loss of fruits, irrespective of treatments,
ppm NAA, and wrapped in poly film reported the slowest increased with the advancement of storage period but, the
weight loss in comparison to unpacked fruits (Kamthe et al., minimum (20.99%) physiological weight loss was recorded in
2004)[22]. The pulp was extracted from custard apples and the fruits treated with sago 5% and maximum (39.11%) was
stored for a period of six months by the addition of 1500ppm observed in untreated fruits at 8 days of storage. The fruits
of potassium metabisulphite (Sravanthi et al., 2004) [23]. The treated with a fine coating of sago (10%) retained excellent
shelf life of custard apple fruits can be extended up to 7 days fruit quality with highest TSS (30ºBrix) and higher sugar
when treated with waxol or waxol + KMNO4 or waxol + NAA (total and reducing sugar) (31.19 and 17.28) at 8 days of
and packed in wrapping poly film of 75 gauge at ambient storage. The minimum sugar (total and reducing sugar) i.e.
storage conditions as against 4 days in untreated and 21.11% and 9.10%, respectively and maximum vitamin ‘C’
unpacked fruits (Masalkar and Garande, 2005) [24]. The seeds content (47.10 mg/100g of pulp) was recorded in the fruits
from the pulp were extracted by machine and manually. The treated with Cacl2 (1.5%) whereas, control treated fruits
extracted pulp was treated whit ascorbic acid (0.25%) and recorded the least (23.10 mg/100g pulp) at 8 days of storage
potassium metabisulphite (0.1%) and stored in the deep and marketable fruits were available even up to eight days of
freezer for six months. Results showed that pulp separated by storage (Gohlani and Bisen, 2012) [30]. Silva et al., (2012) [31]
any method should be stored at -20 °C with 0.1% KMS for packed the custard apple fruits in polyvinyl chloride (PVC)
180 days without spoilage (Bakane et al., 2015) [25]. To film, packed in expanded polystyrene trays enveloped in PVC
preserve the custard apple pulp in the deep freezer for a film and unpacked and tested during a storage period of 0, 4,
period of three months, a combination of chemical 8, 12 and 16 days at 10 °C. The results showed that the
preservative and pasteurization treatments was used. The modified atmosphere allowed for a shelf life of 12 days at 10
results revealed that chemical constituents of the pulp viz., °C. To know the effect of polypropylene package (flushed
TSS, reducing sugar, total sugars and non-enzymatic with different concentration of O2+ CO2 gasses or air) on shelf
browning were found to increase however non-significant life and quality of custard apple pulp was studied. It was
difference were recorded in total sugar. There was a marginal concluded that comparatively higher shelf life of fruits i.e.
increase in microbial load (TBC) in custard apple pulp during 12.28 days with fruits packed in polypropylene bags flushed
storage (Swetha et al., 2017) [26]. Freshly harvested uniform with air (Venkatram et al., 2013) [32]. Custard apple fruits of 2
sized custard apple fruits were washed, cleaned and treated different varieties were packed in polypropylene bags having
with different levels of CaCl2 (1.0, 1.5 and 2.0%) and Sago (5, a different number of pores and stored at 13 °C temperature
10 and 15%) and covered with and without wrapping (Shailaja et al., 2015) [33]. An experiment was conducted to
materials (Newspaper and Banana leaves) and stored at study the impact of modified atmosphere packaging using
ambient temperature. The result revealed that application of three different kinds of films along with low-temperature
sago (10%) + Newspaper wrapping improved the chemical storage at 8, 12 and 15 °C and observations were recorded at
constituents i.e. TSS (27.06 °Brix), reducing and total sugars weekly interval. The study indicated there was a drastic
i.e. 26.16%, 31.80%, respectively while, acidity content reduction in physiological loss of weight, an increasing trend
(0.22%) was minimum in fruits treated with sago (5%) + in the respiration rate and ethylene production rate as well,
Newspaper wrapping (Afzal and Patel, 2018) [27]. and the rate increased with increase in storage temperature
and duration (Patil et al., 2015) [34]. Table 3 shows the
Effect of packaging summary of factors affecting the storability of the custard
The fruits covered in polyethylene bags with wheat straw, the apple.
least loss in fruit diameter and weight occurred. However,

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International Journal of Chemical Studies

Table 3: Summary of factors affecting the storability of custard apple


Factors Findings Author
 Temperatures between 15 and 20 °C, low oxygen and ethylene tensions coupled with 10% Broughton and Guat,
carbon dioxide and a relative humidity of 85%–90% (1979) [12]
 Higher the temperature was, the more rapid the increase in glucose, fructose and sucrose Chang et al., (1999)
[8,13]
concentration.
 Storage temperature between 15 and 20 °C was found to be safe, with maximum shelf life at 15 Prasanna et al.,
°C. (2000) [14]
Effect of temperature
during storage  Steam heating of custard apple pulp at 83 °C temperature for 2 minutes leads to complete Pawar et al., (2011)
[17]
inhibition of PPO activity.
 The shelf life of fruits were 4, 7, 10 days with 22-28 °C (room temperature), 15 °C, 4 °C,
Cheng et al., (2013)
temperatures respectively.
 Pulp stored at -4 and -18 °C temperature showed an increase in sensorial quality viz.
Kamble and Soni,
Appearance, colour, flavour, texture, and overall acceptability and it can be stored up to 70
(2010) [16]
days.
 Citric acid 1% in combination with 0.1% of sodium benzoate, while the addition of 50–100
Sastry et al., (1961)
ppm of sulphur dioxide to check the pink discolouration due to enzymic activity.
 The combination of preservatives viz., 2% glycerol + 1% propylene glycol + 10% glucose Chikhalikar et al.,
syrup + 10% maltodextrin had the highest half‐ life of 75.33 days for ascorbic acid degradation. (2000) [20]
 Custard apple pulp treated with a sugar concentration of 1:4 shows the best results. Kolekar, (2002) [21]
Effect of chemical
treatment  The shelf life of custard apple fruits can be extended up to 7 days when treated with waxol or Masalkar and
waxol + KMNO4 or waxol + NAA. Garande, (2005) [24]
 Pulp separated by any method should be stored at -20 °C with 0.1% KMS for 180 days without Bakane et al., (2015)
[25]
spoilage.
 Fruits treated with 6% Waxol and 30 ppm NAA, and wrapped individually in a poly film Kamthe et al.,
recorded the slowest weight loss. (2004) [22]
 The shelf life of custard apple fruits was 9 days when wrapped with tissue papers and kept in Patil et al., (2011)
[29]
cardboard boxes when stored in zero energy cool chamber.
 The use of packaging films significantly reduced weight loss, preserved firmness and colour of Gohlani and Bisen,
the fruit. (2012) [30]
Silva et al., (2012)
 The modified atmosphere allowed for a shelf life of 12 days at 10 °C. [31]
Effect of packaging
 The higher shelf life of fruits i.e. 12.28 days obtained with fruits packed in polypropylene bags Venkatram et al.,
flushed with air. (2013) [32]
 Drastic reduction in physiological loss of weight, an increasing trend in the respiration rate and
ethylene production rate as well, and the rate increased with an increase in storage temperature Patil et al., (2014)
and duration.

Value addition in custard apple and do not overreact the body's insulin output like processed
The jam, fruit-flavoured yoghurt, syrups and fruit drinks etc. sugars. The sugars designed by nature are complemented by
with fruit extracts from custard apple have been opined other natural products such as vitamins, minerals, fibre and
(Hoyos, 1980) [35]. Sravanthi et al., (2014) [36] prepared protein necessary for energy production and can be used for
various products viz., squash and nectar from custard apple preparing low fat ice creams (Gaikwad et al., 2014) [39].
pulp and stored products at room temperature and cold
storage (5-10 °C). The products were analyzed chemically, Milkshake
microbiologically and organoleptically before storage. Table Custard apple milkshake has good potential to capture
4 shows the summary of processing and value addition of popularity due to its therapeutic and nutritive benefits. The
custard apple. custard apple milkshake prepared from 90 parts of buffalo
milk and 10 parts of custard apple pulp were the most
Juice acceptable and ranked between like very much to like
For the preparation of juice, custard apple pulp can be extremely (Poul et al., 2009) [40]. Custard apple milkshake
extracted with a screw press, basket press or simple hand was prepared from different pulp i.e., 100:0, 90:10, 80: 20,
pressing; juice is strained through muslin cloth which is 75:25, 70:30, 65:35, 60:40. The of custard apple pulp (35%)
clarified by adding 1.4 gm of PVP (Poly Vinyl Pyrrolidone) with cow milk (65%) resulted in most acceptable milkshake
per litre of juice. Juice is boiled with sugar and cooled. 10g and rank between like very much to like extremely (Bakane et
sodium benzoate is added as a preservative (Sinthiya and al., 2016) [41].
Poornima, 2017) [37].
Vinegar
Ice cream Preparation of vinegar from custard apple pulp took 30 days.
Yadav et al., (2010) [38] analyzed chemical composition, Custard apple vinegar had physiochemical characteristics of
quality and cost of ice-cream prepared from custard apple 1.019 gm/ml specific gravity, 1% alcohol content, 5.39%
pulp. It was inferred that ice-cream prepared with the (v/v) acetic acid, 2.0°Brix, and pH of 2.8. The overall
incorporation of 15% custard apple pulp and 15% sugar level acceptability of custard apple vinegar was rated as like very
had overall acceptability of 8.05 scores of hedonic scale. The much on the nine-point hedonic scale as compared to the
production cost and energy value per kg of custard apple ice- market vinegar samples which was rated as like extremely on
cream was ` 61.42 and 97.27 Kcal/100 g, respectively. the nine-point hedonic scale (Raichurkar and Dadagkhair,
Custard apple pulp provides sugars that give sustained energy 2017) [42].
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International Journal of Chemical Studies

Table 4: Summary of processing and value addition of custard apple


Value
Findings Author
addition
Products  Jam, fruit-flavoured yoghurt, fruit drinks and syrups etc. with fruit extracts from custard apple have
Hoyos, (1980) [35]
been suggested.
 Various products viz., squash and nectar are prepared from custard apple pulp and prepared products Sravanthi et al., (2014)
[36]
were stored at the room.
Juice Sinthiya and Poornima,
 Sodium benzoate (10g) is added as a preservative.
(2017) [37]
Ice cream  Ice-cream prepared with the incorporation of 15% custard apple pulp and 15% sugar level had
Yadav et al., (2010) [38]
overall acceptability of 8.05 scores of hedonic scale.
Milkshake  Custard apple milkshake prepared from 90 parts of buffalo milk and 10 parts of custard apple pulp
Poul et al., (2009) [40]
was the most acceptable and ranked between like very much to like extremely.
 That blending of custard apple pulp (35%) with cow milk (65%) resulted in most acceptable
Bakane et al., (2016) [41]
milkshake and rank between like very much to like extremely.
Overall acceptability of the market vinegar samples was rated as like extremely on the nine-point hedonic Raichurkar and
Vinegar
scale, while custard apple vinegar was rated as like very much on the nine-point hedonic scale Dadagkhair, (2017) [42]

From the aforementioned analysis, it is quite evident that 7. Hodges RJ, Buzby JC, Bennett B. Postharvest losses and
various post-harvest practices influenced the storage and shelf waste in developed and less developed countries:
life of custard apple fruits. By storing the fruits at 15-20 °C opportunities to improve resource use. Journal of
coupled with low oxygen and ethylene tensions and 10% Agricultural Science. 2011; 149:37-45.
carbon dioxide significantly adds the shelf life of fruit. The 8. Chen W. Effect of harvest time on the storage quality of
fruit weight loss was higher at higher storage temperatures custard apple fruit. South China Fruits. 1999; 28(4):27.
than the fruits stored at lower storage temperatures. By 9. Bolivar FN, Solis PC, Sauri DS, Saucedo VE. Ripening
treating custard apple pulp with ascorbic acid (0.25%) or of sugar apple fruits (Annona squamosa L.) developed in
potassium metabisulphite (0.15%) or citric acid (1.0%) and Yucatan, Mexico. Agrociencia. 2009; 43:133-141.
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