Scribd Logo
Încărcați

Bine ați venit la Scribd!

  • Kimball Et Al 2019, Figure 1

    Încărcat de

    Abby Kimball
    14 vizualizări1 pagină
    The document summarizes the results of an intracellular cytokine staining (ICCS) experiment analyzing CD4 T cell responses. It shows that CD4 T cells stimulated for ICCS can be divided into four subsets based on IFNγ and TNFα expression: IFNγ- TNFα- (47.9%), IFNγ- TNFα+ (7.42%), IFNγ+ TNFα- (15.6%), and IFNγ+ TNFα+ (29.2%). Dimensionality reduction using tSNE further illustrates the cellular diversity among these subsets.

    Descriere originală:

    High-dimensional analysis of CD4 T cells elicited during primary gHV68 infection

    Titlu original

    Kimball et al 2019, Figure 1

    Drepturi de autor

    © © All Rights Reserved

    Formate disponibile

    PDF, TXT sau citiți online pe Scribd

    Vi se pare util acest document?

    Este necorespunzător acest conținut?

    Raportați acest document
    The document summarizes the results of an intracellular cytokine staining (ICCS) experiment analyzing CD4 T cell responses. It shows that CD4 T cells stimulated for ICCS can be divided into four subsets based on IFNγ and TNFα expression: IFNγ- TNFα- (47.9%), IFNγ- TNFα+ (7.42%), IFNγ+ TNFα- (15.6%), and IFNγ+ TNFα+ (29.2%). Dimensionality reduction using tSNE further illustrates the cellular diversity among these subsets.

    Drepturi de autor:

    © All Rights Reserved
    Descărcați ca PDF, TXT sau citiți online pe Scribd
    Indicator pentru conținut neadecvat
    14 vizualizări1 pagină

    Kimball Et Al 2019, Figure 1

    Încărcat de

    Abby Kimball
    The document summarizes the results of an intracellular cytokine staining (ICCS) experiment analyzing CD4 T cell responses. It shows that CD4 T cells stimulated for ICCS can be divided into four subsets based on IFNγ and TNFα expression: IFNγ- TNFα- (47.9%), IFNγ- TNFα+ (7.42%), IFNγ+ TNFα- (15.6%), and IFNγ+ TNFα+ (29.2%). Dimensionality reduction using tSNE further illustrates the cellular diversity among these subsets.

    Drepturi de autor:

    © All Rights Reserved
    Descărcați ca PDF, TXT sau citiți online pe Scribd
    Indicator pentru conținut neadecvat
    din 1

    CD4 T cells (191+ 193+ 195- CD3+ CD4+ MHCII- events), stimulated for ICCS

    A. Colored by cluster ID: Colored by cluster ID:

    IFNγ+ TNFα- IFNγ+ TNFα+


    15.6 ± 1.87% 29.2 ± 4.91%

    tSNE2
    tSNE2

    tSNE1 tSNE1

    Colored by cluster ID:


    IFNγ expression Colored by cluster ID:
    IFNγ- TNFα- IFNγ- TNFα+
    47.9 ± 4.20% 7.42 ± 1.11%

    TNFα expression
    tSNE2

    tSNE2
    tSNE1 tSNE1

    B.
    Cellular diversity among CD4 T cells defined by cytokine profile
    Colored by cytokine profile:
    IFNγ- TNFα- IFNγ- TNFα+ IFNγ+ TNFα- IFNγ+ TNFα+

    tSNE2

    tSNE1
    Events equally represented in IFNγ- and IFNγ+ subsets
    Events underrepresented in IFNγ+ subsets
    Events only present in cytokine producing subsets
    Events overrepresented in IFNγ+ subsets

    C. Cluster #1
    Cluster #7
    Cluster #10
    Events equally represented High
    Cluster #11
    in IFNγ- and IFNγ+ subsets
    Cluster #13
    Cluster #14
    Median Expression

    Cluster #15

    Cluster #2
    Cluster #3
    Cluster #4 Events underrepresented
    Cluster #5 in IFNγ+ subsets
    Cluster #6

    Cluster #9 Events only present in cytokine Low


    producing subsets
    Cluster #8
    Cluster #12
    Events overrepresented
    in IFNγ+ subsets
    Cluster #16

    Kimball et al
    Tbet
    TNFα
    Lag3
    IkBa
    IL−4
    IL−6
    Ki67
    PD−L1
    IRF4
    FoxP3
    IL−10
    IF Nγ
    CD127
    GITR
    IL−2
    CD69
    CD25
    CTLA4
    PD−1
    CD44
    ICOS

    Figure 1

    S-ar putea să vă placă și