CD4 T cells (191+ 193+ 195- CD3+ CD4+ MHCII- events)

A. B.
anti-CD3 Ab injection γHV68 infection
(spleen) (lung) IL10gfp Expression
#11
20 #20
2.0

IL10gfp Median Expression

1.5
0 #10
#5
#12
1.0

0 2.5
-20
tSNE2

0.5
-20 0 20 -20 0 20
tSNE1 Average IL10gfp Expression
0.0

5
20
10
11
12
8
19
2
15
9
7
18
1
4
16
3
17
14
13
6
Cluster ID

C. 26,602 IL10gfp+ events

2,664 IL10gfp+ events anti-CD3 γHV68

IL10gfp+ Clusters
D. Cluster #11 Conserved in both conditions

Cluster #12
% s 6 Dominant in γHV68
o f e v ent Cluster #20

Cluster #10 Dominant in anti-CD3

Median Expression

%o
f ev ents
2
IL10gfp+ Clusters
Cluster #5 (<1%)
% of CD4 T cells

Cluster #10
Cluster #11 0
FoxP3
PD1
CD44
Lag3
Ki67
IRF4
GITR
ICOS
CTLA4
Tbet
CD117
CD27
CD5
IkBa
CD25
Cluster #12 CXCR3
Cluster #20

Cellular Markers

Kimball et al
Figure 3