Lung: Live, Singlet, CD45+ Events Colon: Live, Singlet, CD45+ Events

(191+ 193+ 195- CD45+ events) (191+ 193+ 195- CD45+ events)

(A) B6 IL10KO (B) B6 IL10KO

20 20

0 0

-20 -20
tSNE2

tSNE2
-20 0 20 -20 0 20 -20 0 20 -20 0 20
tSNE1 tSNE1

(C) (D)
B6 IL10KO B6 IL10KO
Colored by phenotype:
Colored by phenotype:
CD3+ CD4+ CD8- MHCII-
20 CD3+ CD4+ CD8- MHCII-
20 CD3+ CD4- CD8+ MHCII-
CD3+ CD4- CD8lo MHCII-
CD19+ MHCII+
CD19+ MHCII+
CD11b+ Ly6C+ Ly6G+ MHCII- 0
0 CD11b+ Ly6C+ Ly6G+ MHCII-
CD11b+ Ly6C- Ly6G- MHCII-
CD11b+ Ly6C- Ly6G- MHCII-
CD64+
CD64+
-20 CD11c+ CD64- -20
tSNE2

CD11c+ CD64-
tSNE2

CD45+ MHCII+
CD45+ MHCII+
CD45+ MHCII-
-20 0 20 -20 0 20 -20 0 20 -20 0 20 CD45+ MHCII-
NKp46+
tSNE1 tSNE1

(E) B6 IL10KO (F) B6 IL10KO

PD-1 PD-1 CD11c CD11c
25 B6
B6
** #14 3.5 #14 4.5* 25 4 4
IL10KO
20 **
% of events

IL10KO
20
% of events

15 0 0
0 0 15 #13 #13
10
Lag3 Lag3 10 CD103 CD103
5 *** #14 4 #14 4.5* 3.5 4*
5
0
CD64+ CD4+ 0 0 0
Cluster #25 Cluster #14 0 0 CD64+
#13 #13
Cluster #13

Kimball et al
Figure 4