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A. PD-L1 MHCI MHCII CMT + Vehicle (N=3) 10 0 1 0 1.25 0 1.25
A.
PD-L1
MHCI
MHCII
CMT + Vehicle (N=3)
10
0
1 0
1.25 0
1.25
pSTAT1
pERK 1/2
pp38
0
0
1.5
0
2.5 0
4
-10
IkBa
Ly6A/E
Ly6C
-10
0
10
tSNE1
0
1.5 0
3.5 0
3
tSNE2
PD-L1 MHCI MHCII CMT + IFNγ (N=3) 10 0 1.5* 0 3* 0 5* pSTAT1
PD-L1
MHCI
MHCII
CMT + IFNγ (N=3)
10
0
1.5* 0
3*
0
5*
pSTAT1
pERK 1/2
pp38
0
0
2.5* 0
2.5 1
4
-10
IkBa
Ly6A/E
Ly6C
-10
-20
0
0
10
20
tSNE1
0
1.5 2
4.5* 1
5*
tSNE2
B. C. Clusters dominant in CMT + Vehicle 50 *** CMT + Vehicle ** *
B.
C.
Clusters dominant in CMT + Vehicle
50
***
CMT + Vehicle
**
*
CMT + IFN
40
**
30
Clusters dominant in CMT + IFNγ
20
*
*
**
*
***
10
*
**
**
0
Cluster ID
D.
Protein Expression
Low
High
ns
ns
3
CMT + Vehicle
CMT + IFN
E.
Clusters conserved in both conditions
#2 #3 #4 #5 #6 #7 #8 #9 #12 #14 #15 #16
2
1
ns
ns
Protein Expression
0
Low
High
#1 #10 #11 #13
% of events
% of events
MHCII
MHCII
MHCII
iNOS
iNOS
iNOS
Ly6C
Ly6C
Ly6C
BCL6
BCL6
BCL6
IkBa
IkBa
IkBa
Arginase-1
Arginase-1
Arginase-1
1/2pERK
1/2pERK
1/2pERK
Ki67
Ki67
Ki67
TIGIT
TIGIT
TIGIT
pStat1
pStat1
pStat1
pp38
pp38
pp38
pStat3
pStat3
pStat3
CD122
CD122
CD122
b−Catenin
b−Catenin
b−Catenin
pH3
pH3
pH3
CD11c
CD11c
CD11c
ARPcP
ARPcP
ARPcP
MHCI
MHCI
MHCI
CD24
CD24
CD24
CD11b
CD11b
CD11b
pRb
pRb
pRb
pSHP2
pH2AX
pH2AX
pH2AX
p4EBP1
p4EBP1
p4EBP1
pAKT
pAKT
pAKT
y6AEL
y6AEL
y6AEL
CD44
CD44
CD44
pS6
pS6
pS6
CD117
CD117
CD117
pStat4
pStat4
pStat4
PD-L1
PD-L1
PD-L1

Cluster ID

Cluster #12

Cluster #14

Cluster #16

Cluster #2 Cluster #3 Cluster #4 Cluster #5 Cluster #6 (<1%) Cluster #7 Cluster #8 (<1%) Cluster #9 Cluster #15

Cluster #1 (<1%) Cluster #10 Cluster #11 (<1%) Cluster #13