A.

Pre-splenectomy (n=5) Post-splenectomy (n=5)

20 20

0 0

-20 -20
tSNE2

tSNE2
-20 0 20 -20 -20 0 0 20
20

tSNE1 tSNE1

B. C.
Pre
100
Post
% of total CD45+ cells

80
Pre B cells
60 T cells
Myeloid cells
40 Post
NK and Other cells
20
Day: 19 59 110 203 241
0

Colored by phenotype

D. E.
Pre-splenectomy (n=5) Post-splenectomy (n=5) CCR6 All Clusters CCR6 T Cells CCR6 Myeloid Cells
4 p <0.0001 4 p <0.0001 4 p <0.0001
CCR6 Expression

6 6
Relative Expression

Relative Expression
Relative Expression

3 3 3

2 2 2

1 1 1
0 0
0 0 0
-20 0 20 -20 0 20 Pre Post Pre Post Pre Post

CCR4 All Clusters CCR4 T Cells CCR4 Myeloid Cells

CCR4 Expression

8 8
8 p <0.0001 5
p <0.0001 6 p <0.0001
Relative Expression

Relative Expression
Relative Expression

4
6
4
3
4
0 0 2
2
2
1
-20 0 20 -20 0 20

0 0 0
Pre Post Pre Post Pre Post