mIHC specific analysis using flow cytometry software (FlowJo®)

A. CD4 T cells
FoxP3+
CD8 T cells
B cells
Macrophages
Other

WT #1
1000 1.0K

800

600

500

400

Cell Y Coordinate
Cell Y Coordinate
200

0 0
0 500 1000
0 300 600 900 1.2K
Cell X Coordinate Cell X Coordinate

Post spectral unmixing Graphical representation of inForm x, y x, y coordinates & phenotypes

coordinates & phenotypes plotted in Prism defined and generated by FlowJo

B. 1.0K
Cells associated with bronchiole

IFNγKO #1 800

B220
600 Bronchiole CD8
6.65
400
Cell Y Coordinate

200
CD8 Median Expression B220 Median Expression

23.1 947.1 6.3 5349.7

0
0 300 600 900 1.2K 0 300 600 900 1.2K 0 300 600 900 1.2K
Cell X Coordinate

All events Bronchiole Associated

CD4 T cells
CD8 T cells
B cells
Macrophages
Other

2918 events 194 events