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Symbiosis Host
- living together of unlike/different - harbors the parasite
organisms - may/may not suffer from pathogenic
Symbiont effects of the parasite
- the organism in a symbiotic relationship
Final Host (Definitive Host)
Types of Symbiotic Relationship - Harbors mature/adult form of parasite
Commensalism - Sexual reproduction occurs
- one benefits and one unharmed - Usually man
- non pathogenic
- Entamoeba coli Intermediate Host
Mutualism - harbors immature/larval stage of a
- both organisms benefit from each parasite
other - undergoes asexual reproduction
- Termites and Flagellates in their - lower animals & vegetation
digestive system (synthesize *humans become intermediate hosts in the
cellulase for the breakdown of following examples:
ingested wood) - Plasmodium – causes malaria
Parasitism - Taena solium (tapeworm)
- host is harmed while parasite
benefits Vectors
Phoresis - responsible for parasite transmission
- Means “to carry” o Biologic Vector
- Phoront – being carried parasite develops in it
- no physiologic interaction is involved there’s physiologic
between parasite and host interaction
ex. Aedes, ticks, fleas,
Parasitology mosquito – Anopheles
- deals with the dependence of one
organism to another o Mechanical/Phoretic Vector
- host (animals/humans) & parasite no morphologic change
- study of the relationship between no physiologic interaction
parasites and their host parasite is outside the
vector
* Majority of people inflicted with parasitic ex. Cockroaches, flies
infections are from poor, developing countries.
Accidental Host
Schistosoma (blood-flukes) - harbors parasite that are not infective
- causes enlarged bellies because of fluid - ex. When humans injest Toxocara cati
accumulation (ascarid on cats)
Parasite Stages
Helminthes (adult)
- motile - non-motile
- vegetative/feeding - infective
stage stage
- on watery stool - oval
Parasite in the photos: Giardia lamblia
*Types of Hookworm
- Necator americanus
common name: New world hookworm
(common in America)
- Ancylostoma duodenole
ascaris egg common name: Old world hookworm
(common in Europe)
• Phylum Microsporidia
– Intracellular parasites
– Now classified as fungi
• Subphylum Mastigophora – Flagellates – Spore forming
– Possess Polar tube
ex. Enterocytozoon and Encephalitozoon
*contaminants include:
- urine
- toilet bowl water
o may destroy trophozoite
- Gross Abnormalities
o mucus
o pus
o larva
o worms
o proglottids
o tapeworm segments Direct Wet Mount (less sensitive) » NSS Mount
*indole and skatole – for stool odor » Iodine Mount
- for protozoan cyst
- stains: Lugol’s, D Antoni’s
- trophozoites are killed
» NSS Mount
- for trophozoite
- stain: Nair’s methylene blue or
Quensell’s stain
• Concentration Technique
- needs more amount of stool
- recovers cyst, larva, eggs
Permanent Stains
– Final Procedure
– Use:Confirmation of the Presence of
Protozoan Cyst and/or Trophozoites
– Preservative of Choice:PVA with
Schaudinn’’s
Common Stains
– Trichrome (Wheatley Modification)
» widely used; easy to prepare;
long shelf life
– Iron Hematoxylin – Other Examinations Done using Stool
» time consuming » Kato-Katz (quantitative technique for
helminth eggs)
» excellent morphology of
» Kato-Thick (qualitative
intestinal protozoans
» Recovery of Hookworm and Strongyloides
Specialized Stains
larva
» Modified Acid Fast Stain • BaermannTechnique
• HaradaMoriTechnique
• AgarPlate
» Use of Culture Medium
Blood
• Collected for diagnosis of systemic or blood
borne parasites
– Examinations Done
• Thick and Thin Smears
• Knott’s Concentration
(Philarial worms)
Technique (1ml blood + 10ml 2%
formalin)
• Buffy Coat Slides
(hemoflagellate detection)