SRI SHAKTHI

INSTITUTE OF ENGINEERING AND TECHNOLOGY

L & T BYPASS ROAD, COIMBATORE – 641 062

LESSON PLAN

FACULTY NAME: S.Sivapriyadharshini ACADEMIC YEAR : 2017-18

DEPARTMENT : Biotechnology SEMESTER : IV
SUBJECT CODE : BT6401 CREDITS :3
SUBJECT NAME : Analytical Methods and Instrumentation REGULATION : 2013

QUESTION BANK
UNIT I

PART A

1. List any four elements used in Optical Instruments.

2. What are monochromators?
3. What is electromagnetic radiation?
4. Define wave number.
5. What is signal to noise ratio?
6. Compare the various methods of enhancement of S/N ratio.
7. Compare monochromatic and polychromatic light.
8. Explain the formula for energy of EMR.
9. State the different sources of noise in an optical instrument.
10. Compare signal and noise.

PART B

1. Illustrate the general components of optical instruments with a neat diagram and
describe their uses.
2. Discuss the important characteristics and various ranges of EMR spectrum.
3. Illustrate the general design of radiation source, wavelength selectors, transducers and
signal processors in analytical instruments.

UNIT II

PART A

1. State Beer’s law.

2. Differentiate between absorbance and transmittance.
3. State Lambert’s law.
4. What are the applications of colorimeters?
5. Absorbance and the concentration of a solution are related. Justify.
6. Write the differences between phosphorescence and fluorescence.
7. Justify the use of quartz cuvettes for UV spectroscopy.
8. Give the applications of UV visible spectrophotometers.
9. How is absorption used to measure the concentration of an analyte?
10. Compare transmittance and absorbance.
11. What are the applications of IR spectroscopy?
12. Compare the different sources of radiations used in analytical instruments?
13. What is Absorption Spectroscopy?
PART B

1. Explain with necessary diagrams the instrumentation involved in UV visible

spectrophotometer?
2. Explain the construction and working of UV-Visible Spectrophotometer.
3. Explain with neat diagram the construction and working of atomic absorption
spectrophotometer?
4. Differentiate between flourescence and phosphorescence spectroscopy.
5. Compare and contrast between UV-Visible and IR spectroscopy.
6. Differentiate between absorbance and emission spectroscopy.
7. Compare and contrast between colorimeter and spectrophotometer.
8. Write in detail the principle, instrumentation and applications of absorption
spectroscopy.
9. What is IR spectroscopy? Discuss the important parts and uses of IR spectroscopy.

UNIT III

PART A

1. What is the principle of mass spectrometer?

2. What is NMR?
3. Give a few applications of NMR.
4. What are the types of mass spectrometer?
5. What are the various parts of a mass spectrometer?
6. State the principle of mass analyzer.
7. What is the principle of Electron spin resonance?
8. Define electron paramagnetic resonance.
9. What is g-value?

PART B

1. Explain in detail the NMR Spectrometer.

2. Explain the applications of a 13C NMR.
3. Explain the applications of a 1H NMR.
4. Explain the conceptual aspects of Mass spectrometer with necessary diagram.

UNIT IV

PART A

1. What is chromatogaraphy ?
2. What are the classifications of chromatographic techniques?
3. What are the types of chromatography?
4. What is Retention Time?
5. What is Gas Chromatography
6. List out the types of colummns used in gas chromatography
7. List out the various detectors used in gas chromatography.
8. What are the advantages of Gas Chromatography?
9. Why High pressure pumps are used in HPLC?
10. Distinguish between Thin Layer Chromatography and Column Chromatography?

PART B
1. With a neat block diagram explain the construction and working of gas
chromatography.
2. Explain with a neat diagram the construction and working of capillary electrophoresis..
3. Explain the construction and working of Ion exchange chromatography with a neat
diagram
4. Explain the construction and working of High Pressure Liquid Chromatography
(HPLC) with a neat sketch.
5. Explain the construction and working of thin layer chromatography with a neat
diagram.

UNIT V

PART A

1. Define pH.
2. What are the limitations of glass electrode?
3. What are the advantages of glass electrodes?
4. Explain the principle of ion selective electrodes.
5. What are the limitations of hydrogen electrode?
6. What is reference or calomel electrode?
7. Give a few examples of reference electrodes.
8. What are the applications of voltammetry?
9. Define AFM.
10. Give the principle of scanning probe microscopes.

PART B

1. Explain in detail the glass electrode.

2. Explain in detail the calomel electrode.
3. Explain in detail selective ion electrodes.
4. Explain Standard hydrogen electrode with a neat sketch.
5. Give brief notes on electrochemical cells and comment on the use of glass electrodes
in electrochemical analysis.
6. Give a detailed account on the principle of voltammetry and its application in
electrochemistry.
7. Explain in detail the working and construction of scanning probe microscopes.