PARTIAL CHARACTERIZATION OF PYROLIGNEOUS

ACID EXTRACTED FROM JACKFRUIT PEELS AND ITS

ANTIBACTERIAL POTENTIAL AND BIOEFFICIENCY AS A
SOIL ENHANCER
Matranas, C., Pabilona, C., Rosellas, K.N.

INTRODUCTION METHODOLOGY
Pyroligneous acid is composed of highly complex
PHASE 1
oxygenated brown-colored viscous aqueous liquid
Plant Collection STEP
obtained from the pyrolysis of wood and other plant Jackfruit peels were
collected from the
Department of Agriculture,
01
materials. It can be used as a pesticide, fungicide,
Abuyog, Leyte
insecticide, herbicide, and plant growth enhancer.

In this study, pyroligneous acid extracted from jackfruit STEP PA Preparation

peel will be produced, and its physical and chemical 02 The PA was prepared
through pyrolysis and was
centrifuged to hasten the
components will be determined. This study will also process

investigate its bioefficiency specifically its antibacterial PA Determination

The PA was tested for its STEP
potential against the selected microorganisms: E. coli, physical (pH, boiling range,
specific gravity) and chemical 03
P. aeruginosa, and S. aureus and its efficacy as a soil properties through laboratory
tests and GC-MS, respectively.
treatment on degraded soil samples will be
Antibacterial Test
investigated. STEP The PA was tested for its

04
RESULTS AND DISCUSSION
antibacterial property
through disk diffusion
method

Physical Characterization
The boiling point of the jackfruit peel
Soil Sampling STEP
Soil samples were collected

pyroligneous acid was reported to be in the

at a site in Caloogan, Palo,
Leyte
05
range of 114 – 120.5 °C.
Soil Preparation
The pH of the pyroligneous acid is 3.25, which is STEP Soil that will be analyzed for

the average pH obtained from the 3 trials. 06 its nutrient content were air
dried, pulverized, and sieved
prior to sending it to soil
The corrected specific gravity is 1.0140. facilities (BoS & VSU)

Chemical Characterization PA Application

STEP
A total of 67 compounds were detected from Soil that will be further
analyzed for its nutrient
07
content were treated with
the sample. The identified chemical compounds diff. concentrations of PA
prior to analysis
belong to organic compounds: aldehydes,
ketones, alcohols, phenols, and furan STEP Data Analysis
derivatives. 08 Results were further
discussed and interpreted
according to standard soil
Out of all the chemical constituents of the protocols

pyroligneous acid, about 56% are phenolic PHASE 2

substances. It is composed mainly of organic

acids and phenols. CONCLUSION

Antibacterial Test
Based on the results and findings of this study and the
According to the results, 75% pyroligneous acid
conditions where the study was conducted, the
showed the highest antibacterial activity among
following conclusions can be drawn:
all concentrations against E. coli.
1.       The physical characteristics of the pyroligneous
For S. aureus, only 1 disk was analyzed; 100%
acid are as follows: it has a pH of 3.57, a specific
pyroligneous acid exhibited the highest
gravity of 1.014, and a boiling range from 114 – 120.5
antibacterial activity.
°C.
As for P. aeruginosa, only the positive control
2.       Through disk diffusion method, it is proven that
exhibited an antibacterial activity.
the pyroligneous acid extracted from jackfruit peels
Soil Nutrient Test
has an antibacterial effect against E. coli & S. aureus.
Based on the results of the various tests for soil
3.       The pyroligneous acid does not improve the
nutrient content, the PA did not exhibit a
quality of degraded soils.
significant effect on the soil samples obtained
from Caloogan, Palo, Leyte for any nutrient

(N,P,K,OM). Travero, J. T. & Mihara M. (2015). Impacts of Pyroligneous Acid to Biological

and Chemical Properties of Depleted Soil in Bohol, Philippines. IJERD –

International Journal of Environmental and Rural Development. 6(1).

Souza, JB. G., Ré-Poppi, N. & Rapozo, Jr. JL. (2012). Characterization of

Pyroligneous Acid used in Agriculture by Gas Chromatography-Mass

Spectrometry.  J. Braz. Chem. Soc., 23 (4), 610-617.