1. Which of the following does not act as a restriction enzyme?

a) EcorI
b) BamHI
c) HindIII
d) polydeoxyribonucleotide synthase
View Answer
Answer: d
Explanation: Polydeoxtribonucleotide synthase is a DNA ligase.
2. Which of the following enzyme is responsible for making a DNA copy from RNA?
a) Reverse transcriptase
b) DNA polymerase
c) RNA polI
d) RNA polII
View Answer
Answer: a
Explanation: Reverse transcriptase generates complementary DNA from RNA template.
3. Which of the following statements is true?
a) Exonuclease III removes nucleotide residues from the 3’ ends of a DNA strand
b) Bacteriophage lambda exonuclease removes terminal phosphates
c) Alkaline phosphatase removes nucleotides from 5‘ends
d) Kinase adds homopolymer tails to the 3’ –OH ends of a linear duplex
View Answer
Answer: a
Explanation: Bacteriophage lambda exonuclease removes nucleotides from 5‘ends.
Alkaline phosphatase removes terminal phosphates
Terminal transferase adds homopolymer tails to the 3’ –OH ends of a linear duplex.
4. Which of the following can be termed as a restriction modification system?
a) Restriction endonuclease + methylase
b) DNA ligase + methylase
c) Restriction endonuclease + acetylase
d) DNA ligase + acetylase
View Answer
Answer: a
Explanation: (Restriction endonuclease + methylase) is termed as a restriction modification system.
5. Which of the following is true about restriction endonucleases?
a) Type I and II requires ATP to move along DNA
b) Type I, II and III requires ATP to move along DNA
c) Type II requires no ATP and cleaves DNA within recognition sequence
d) Type II requires ATP and cleaves DNA within recognition sequence
View Answer
Answer: c
Explanation: Type I and III requires ATP to move along DNA.
6. Which of the following enzymes’ combined action leads to the generation of sticky ends in plasmid
vector?
a) Alkaline phosphatase and terminal transferase
b) Exonuclease III and alkaline phosphatase
c) Bacteriophage lambda exonuclease and terminal transferase
d) Exonuclease III and terminal transferase
View Answer
Answer: c
Explanation: The combined action of bacteriophage lambda exonuclease and terminal transferase
lead to the generation of sticky ends in plasmid vector.
7. Generally a plasmid vector contains how many elements?
a) 1
b) 2
c) 3
d) 4
View Answer
Answer: c
Explanation: Cloning site, drug resistant gene and replication origin are the 3 elements in a plasmid
vector.
8. To make the recombinant plasmid permeable to DNA molecules, which of the chemicals is
added?
a) MgCl2
b) CaCl2
c) NaCl
d) HCl
View Answer
Answer: b
Explanation: The cells are treated with CaCl2 to make them permeable to DNA molecules through a
process called transformation.
9. Which of the following statements is false?
a) The cells which take up recombinant plasmids are drug resistant
b) The cells with no recombinant plasmids are drug resistant
c) Plasmid replication is independent of cell’s division
d) The cells containing recombinant plasmid are called transformed cells
View Answer
Answer: a
Explanation: Recombinant plasmid contains a drug resistant gene.
10. The extra chromosomal, self-replicating, closed, double stranded and circular DNA molecule is
generally termed as
a) Chromosome
b) Plasmid
c) Genomic DNA
d) Bacteriophage
View Answer
Answer: b
Explanation: Plasmid is an extra chromosomal, self-replicating, closed, double stranded and circular
DNA molecule.
11. E.cor1 is a
a) DNA ligase enzyme
b) Restriction endonuclease
c) A vector used for insulin synthesis
d) A plasmid used as a vector
View Answer
Answer: b
Explanation: Ecor1 is a restriction enzyme that cleaves vector at the cloning site.
12. In which stage of genetic engineering a probe is used?
a) Cleaving DNA
b) Recombining DNA
c) Cloning
d) Screening
View Answer
Answer: d
Explanation: In hybridization, which is one of the methods of screening, a probe is used.
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13. A DNA library is a

a) A DNA fragment inserted into a vector.
b) A general collection of all genes sequenced thus far.
c) All DNA fragments identified with a probe.
d) A collection of DNA fragments that make up the entire genome of a particular organism.
View Answer
Answer: d
Explanation: DNA library is a collection of DNA fragments representing the entire DNA of an
organism.
14. Which of the following enzyme is required for end to end joining of DNA?
a) DNA ligase
b) Restriction endonuclease
c) RNA polymerase
d) DNA polymerase
View Answer
Answer: a
Explanation: DNA ligase ligates both blunt and sticky ends of DNA.
15. Repressor molecules bind to
a) Operator
b) Promoter
c) Enhancer
d) Hormone response element
View Answer
Answer: a
Explanation: An operator lies partially within the promoter, repressor binds to the DNA at operator
region inhibiting the expression of genes.

1. Which of the following statements is not correct?

a) Bacteria possess only one type of RNA polymerase
b) Attenuation is a regulatory process used by bacteria to control the initiation of transcription
c) Repressor binds to operator
d) Bacterial genes are polycistronic
View Answer
Answer: b
Explanation: Attenuation results in premature termination of transcription.
2. Which of the following bacterial operon is not controlled by attenuation?
a) Arabinose
b) Tryptophan
c) Leucine
d) Histidine
View Answer
Answer: a
Explanation: Arabinose is a carbohydrate.
3. Which of the following is the most appropriate definition of an operator?
a) A non-coding, regulatory DNA sequence that is bound by RNA polymerase
b) A non-coding, regulatory DNA sequence that is bound by a repressor protein
c) A DNA-binding protein that regulates gene expression
d) A cluster of genes that are regulated by a single promoter
View Answer
Answer: b
Explanation: A repressor binds to operator.
4. To which class of transcription factor do nuclear receptors belong?
a) Zinc finger proteins
b) Leucine zipper proteins
c) Helix-turn-helix proteins
d) Helix-loop-helix proteins
View Answer
Answer: a
Explanation: Nuclear receptors belong to zinc finger motif.
5. Which of the following statements about lac operon in e.coli is true?
a) Promoter is the binding site for the lac repressor
b) Operon is only switched on in the absence of lactose in the growth medium
c) β-galactosidase is only produced in large quantities when the lac repressor is bound to the
operator
d) Lac operon mRNA is a polycistronic mRNA
View Answer
Answer: d
Explanation: Bacterial genes are polycistronic and genes in eukaryotes are monocistronic.
6. Which of the following statements regarding the regulation of trp operon expression by attenuation
is correct?
a) Rapid translation of the leader peptide prevents completion of mRNA transcript
b) Rapid translation of the leader peptide allows completion of mRNA transcript
c) The leader peptide sequence encodes enzymes required for tryptophan synthesis
d) The leader peptide sequence contains no tryptophan residues
View Answer
Answer: a
Explanation: Attenuation results in premature termination of transcription.
7. Which of the following increases gene expression as much as 200-fold?
a) TATA box
b) Insulator
c) Enhancer
d) CAAT box
View Answer
Answer: c
Explanation: Only enhancers can increase gene expression as much as 200-fold.
8. The mechanism by which CBP activates transcription is
a) CBP has DNA methyl transferase activity
b) CBP has histone acetyl transferase activity
c) CBP interacts with the basal transcription complex
d) CBP interacts with the basal transcription complex and has histone acetyl transferase activity
View Answer
Answer: c
Explanation: CBP interacts with the basal transcription complex and activates transcription.
9. RNAi stands for
a) RNA inducer
b) RNA interference
c) RNA intron
d) RNA insertion
View Answer
Answer: b
Explanation: RNAi stands for RNA interference.
10. Which of the following about mRNA stability is not correct?
a) Regulation of mRNA stability is a way of regulating gene expression
b) Prokaryotic mRNAs have a half-life of only a few minutes
c) Histone mRNAs have especially long poly-A tails and are stable
d) It is thought that poly-A tails stabilize eukaryotic mRNAs
View Answer
Answer: c
Explanation: Histone mRNAs lack poly-A tails so they are unstable.