Formulation KRISI5:

Cream Of The combination of Betel Leaf And Leaves Pomegranate As Anti-Aging and Anti-
Acne

FORMULATION (KRISI5) CREAM OF THE COMBINATION OF BETEL Commented [u1]: Pakai template yang sudah disediakan jurnal
Commented [u2]: Langsung bahasa latin
LEAF AND LEAF DELIMA AS ANTI-AGING AND ANTI-ACNE
Commented [u3]: leaves
Commented [u4]: bahasa latin diikuti leaves

Radiatul Fadilah Putri1, Mardiyah Syafitri1, Iin Andini br Sinuhadji1, Marianne2*

1
Department of Pharmaceutical Technology, Faculty of Pharmacy, Universitas
Sumatera Utara, Medan 20155, Indonesia
2
Department of Pharmacology Pharmacy, Faculty of Pharmacy, Universitas Sumatera
Utara, Medan 20155, Indonesia
*
Email: marianne80@usu.ac.id

Abstract
The appearance of wrinkles which become one of the signs of aging, and acne
on the face are the factors most disturbing beauty of the face. Aging is triggered by free
radicals in the body, this occurs due to oxidative processes. Unlike the pimples that
appear on the face, one of which is caused by the activity of bacteria that cause acne is
Propionibacterium acnes. Many Indonesian natural materials that have potential in the
field of beauty and good bioactive components that have not been used optimally.
Pomegranate leaves (Punica granatum L) have been studied have a high antioxidant
because it contains elagat acid in high amounts, while the betel leaf (Piper betle L. ) has
antibacterial activity against Propionibacterium acnes bacteria that cause acne. Topical
preparations of anti-aging and anti-acne already widely circulated in the market in the
form of a cream, because it is considered to have aesthetic appeal greater because it is
not greasy and the ability to absorb into the skin at the time of basting. The purpose of
this research is to produce a cream which is effective as an anti-aging and anti-acne that
can be produced for cosmetic preparations so that consumers can feel two facial care
benefits in one product at an affordable price. Because it is considered to have aesthetic
appeal greater because it is not greasy and the ability to absorb into the skin at the time
of basting. The purpose of this research is to produce a cream which is effective as an
anti-aging and anti-acne that can be produced for cosmetic preparations so that
consumers can feel two facial care benefits in one product at an affordable price. The
method used in anti-aging test is using a skin analyzer and moisture checker and for
 Formulation KRISI5:
Cream Of The combination of Betel Leaf And Leaves Pomegranate As Anti-Aging and Anti-
Acne

anti-acne is agar diffusion method using paper pencadang by measuring the diameter of
growth inhibition against Propionibacterium acnes bacteria. Commented [u5]: Ringkas menjadi 200 kata saja. Terdiri dari
latar belakang dan tujuan, metode, hasil dan kesimpulan
Keywords : Anti-acne, anti-aging, pomegranate leaf, betel leaves Commented [u6]: leaves

1 INTRODUCTION
Has a beautiful face and naturally became one of happiness and is one of the factors Commented [u7]: paragrafnya tidak masuk seperti ini
Commented [u8]: Having
to increase the confidence of every woman. In this modern era, women are required to
Commented [u9]: natural
look beautiful so that directs women to try to get the perfect beauty. Commented [u10]: become
The appearance of wrinkles which become one of the signs of aging, and acne on Commented [u11]: therefore

the face are the factors most disturbing beauty of the face. Aging is triggered by free
radicals in the body. This occurs due to oxidative processes. The use of anti-aging
antioxidant-containing compound is an effort that is often done to prevent or at least the
age of aging naturally (Ardhie, 2011). Commented [u12]: cara menulis rujukan mengunakan angka.
Jadi ini angka 1
Antioxidants are one of the compounds that can neutralize and reduce free radicals
and inhibit the oxidation of cells thereby reducing the occurrence of cell damage, such
as premature aging (Heranani and Raharjo, 2005). Commented [u13]: 2

Unlike the pimples that appear on the face, one of which is caused by the activity of
bacteria that cause acne is Propionibacterium acnes. Required to eliminate or prevent Commented [u14]: revise

anti-acne that is to kill and inhibit the growth of bacteria.

Variety of natural ingredients such as carrots, gotu kola, rosella, yam has been
widely used in the formulation of beauty products such as masks, moisturizers, body
lotions and so on. Many Indonesian natural materials that have potential in the field of
beauty and good bioactive components, but has not been utilized optimally.
Pomegranate leaves have been studied have a high antioxidant as pomegranate leaf
extract (Punica granatum leaf) showed that pomegranate leaf elagat acid containing
high amounts, which elagat acid is one of the free-radical scavengers (Amjad, 2012). Commented [u15]: revise sentence
Commented [u16]: 3 kalau bisa rujukannya yang bahasa inggris
The betel leaf (Piper betle L.) have antibacterial activity, one of them against
Commented [u17]: leaves
Propionibacterium acnes bacteria that cause acne. From previous studies it is known Commented [u18]: how to write?
that ethanol extracts of betel leaf have KBM (Kill Minimum Levels) 0.25% (w / v) Commented [u19]:

against Propionibacterium acnes (Fadila, 2010).

Cream is a semisolid dosage forms contain one or more ingredients dissolved or
dispersed in an appropriate base material (MOH, RI., 1995). Cream considered more
 Formulation KRISI5:
Cream Of The combination of Betel Leaf And Leaves Pomegranate As Anti-Aging and Anti-
Acne

aesthetic appeal to have a greater because it is not greasy and the ability to absorb into
the skin at the time of application of (Ansel, 1989).
Based on the above, it is necessary to do research that is able to identify the
potential of the combination of pomegranate leaf and betel leaf as an anti-aging and
anti-acne creams formulated in dosage forms.
2 RESEARCH METHODS Commented [u20]: EXPERIMENTAL

Tools Commented [u21]: Instrument. Yang penting saja yang

digunakan untuk alat ukur
The tools used in this research is analytical balance, pH meter, drying cabinets,
blender, mortar, stamfer, the object glass, dark bottles, rotary evaporator, plate
evaporator, glass beaker, flask, pipette, measuring cups, cover plastic pots, tools filter,
filter paper, parchment paper, bath water, stir bar, spatula, plastic pots, transparent tape,
label, skin analyzer and mouisture checker.
Material
Materials used in this study include herbs and chemicals. Plants that are used are the
leaves of pomegranate and betel leaf and chemicals are chemicals used include stearic Commented [u22]: Pakai bahasa latin dan jelaskan dari mana
diperoleh. Apakah ada dideterminasi?
acid, cetyl alcohol, triethanolamine (TEA), sorbitol, propylene glycol, glycerin, distilled
water, nipagin, sodium metabisulfite, perfume, silica gel, methylene blue, n-hexane,
methanol, chloroform, distilled water. Commented [u23]: Jelaskan sumbernya apakah merck atau
sigma aldrich
2.3 Time and Place of Execution
This study was initiated on 11 April 2017. The study was conducted at the
Laboratory of Phytochemistry, Cosmetology and Microbiology, Faculty of Pharmacy,
University of North Sumatra, Medan.
2.4 Research Procedures Commented [u24]: Tidak pakai nomor

2.4.1 Sampling
Sampling was done by purposive ie without comparing with other regions.
Pomegranate leaf samples (Punnica granatum) taken from Jalan Rambutan Sentang
Village, District East Range, Asahan, North Sumatra. Samples of betel leaf (Piper betel
L) were taken from Padangsidimpuan, Padangsidimpuan District of North, North
Sumatra. Commented [u25]: Masukkan ke material. Tidak perlu nama
jalan hanya daerahnya saja atau kabupaten
2.4.2 Identification of Samples
 Formulation KRISI5:
Cream Of The combination of Betel Leaf And Leaves Pomegranate As Anti-Aging and Anti-
Acne

Identification of plants is done in Medanense Herbarium Laboratory of Plant

Taxonomy Department of Biology, University of Medan, North Sumatra. Commented [u26]: Masukkan ke material juga. Sebutkan
nomor surat determinasinya
2.4.3 Sample Processing
a. Pomegranate leaf processing
Pomegranate leaf carefully washed with distilled water. Then dried in the sun and
ground shielded using an electric blender to a powder.
b. Betel leaf processing
The betel leaf carefully washed with distilled water. Then dried in the sun and
ground shielded using an electric blender to a powder. Commented [u27]: Dibuat simpel, disatukan saja penjelannya

2.4.4 Pomegranate Extract Ethanol manufacture and Betel Leaves

Pomegranate leaf and betel leaf that has become a powder, each put in a dark
bottle maceration with 96% ethanol for 3x24 hours. Results maceration filtered and then
the solvent was evaporated with a rotary evaporator in order to obtain a thick extract. Commented [u28]: Satukan dengan atas

2.5 Characterization Simplisia Pomegranate and Betel Leaves

2.5.1 Organoleptic dan macroskopic Commented [u29]: macroscopic

Organoleptic testing conducted on simplisa pomegranate leaf and betel leaf

includes examining the smell, color and taste. Macroscopic examination of pomegranate
leaf and betel leaf includes the shape, length and width.
2.5.2 Characterization of the assay Commented [u30]: extract

Characterization of the determination of water content, water soluble extract

content, content of ethanol soluble extract, total ash and acid insoluble ash content.
2.6 Phytochemicals Screening Examination
Screening fitokima pomegranate leaf extract and betel leaf includes examining
alkaloids, saponins, flavonoids, triterpenoids.
2.7 Preparation of Formula Basic Cream (Young, 1972) and the preparation of a
cream
R/ stearic acid 12 g
cetyl alcohol 0.5 g
sorbitol 5g
propylene glycol 3g
triethanolamine 1g
 Formulation KRISI5:
Cream Of The combination of Betel Leaf And Leaves Pomegranate As Anti-Aging and Anti-
Acne

nipagin 0.1 g
sodium metabisulfite 0.1 g
Perfume 3 drops
Distilled water ad 100 ml Commented [u31]: jadikan satu kalimat

Table 1. Formula Cream preparations Betel leaves and leaves Pomegranates

Consentration (%)
Material
F0 F1 F2 F3 F4
Extract
- 2,5 5 10 10
Pomegranate Leaf
Extract Betel Leaf - 2,5 10 5 10
Basic cream 100 95 85 85 80 Commented [u32]: perhatikan cara mereka membuat tabel
tabel dan gambar dibuat terppisah dari tulisan
kalau bisa formulanya di sebelah kiri, komposisinya di sebelah
kanan
How to: stearic acid, cetyl alcohol, incorporated into the vaporizer cup and
melted over a water bath (mass I). Nipagin and sodium metabisulfite dissolved in hot
water, then add TEA and shaken until dissolved (mass II). Then add mass II into the
first mass in the heat as he crushed in a mortar continuously and add sorbitol 70% to
form a cream base. Pomegranate leaf extract and crushed betel leaf extract with the
addition of propylene glycol, then add the cream base gradually while crushed until
homogeneous. Finally add 3 drops of perfume and crushed until homogeneous.

2.8 Determination of Quality Physical Preparations

2.8.1 Homogeneity Examination
A certain amount of preparation applied to the glass object, the preparation must
demonstrate a homogeneous composition and no visible coarse (MOH, 1995).
2.8.2 Determining pH Preparations
pH meter calibrated in advance by using a standard neutral buffer solution (pH
7.01) and acidic pH buffer solution (pH 4.01) until the instrument shows the pH values.
Kemudiaan electrode was washed with distilled water, then dried with a tissue. Samples
were prepared in a concentration of 1% that weighed 1 gram dosage and dissolved in
100 ml of distilled water. Then, the electrode is dipped in the solution. Left tool shows
 Formulation KRISI5:
Cream Of The combination of Betel Leaf And Leaves Pomegranate As Anti-Aging and Anti-
Acne

the pH to a constant price. The figures shown pH meter is a pH dosage.

2.8.3 Determination of emulsion type preparations
A certain amount of preparation put on the object glass, add 1 drop of methyl
blue, stirred with a stirring bar. If the blue methyl spread evenly means such stocks
emulsion type O / W, but when only blue spots means such stocks emulsion type A / m
(MOH, 1995).
2.8.4 Stability Preparations Observations
Each formula preparation put into 25 ml measuring cup, covered with plastic
uppers. Furthermore, the storage was observed at 1, 2, 3, and 4 weeks conducted at
room temperature, which is observed as part of rupture or failure of the emulsion, phase
separation, discoloration and odor of the preparation.
2.8.5 Irritation Test Against Volunteers
This experiment was carried out on five volunteers, dabbed behind the ears,
kemudiaan leave for 24 hours and see the changes that occur in the form of skin
irritation, itching and coarsening (Wasitaatmadja, 1997).
2.9 Determination of Effectiveness of preparations
2.9.1 Testing Volunteers Against Anti-aging
All the volunteers were measured prior initial condition skin backs of hands or on
a test area that has been marked by various Pameter test, such as water content
(moisture), smoothness (evenness), large pore (pore), the number of spots (spot),
wrinkles (wrinkle ) and the depth of wrinkles (wrinkle's depth) using skinanalyzer and
moisture checker tool.
Use cream began with basting until evenly every twice a day, at night and in the
morning every day for 4 weeks in the temple area of the eye. Changes in skin condition
was measured every week for 4 weeks by using a skin analyzer and moisture checker.
Testing the effectiveness of anti-aging conducted on volunteers as many as 15 people
and is divided into five groups, namely:
group I : 3 volunteers were given cream with F0
group II : 3 volunteers were given cream with F1
group III : 3 volunteers were given cream with F2
group IV : 3 volunteers were given cream with F3
 Formulation KRISI5:
Cream Of The combination of Betel Leaf And Leaves Pomegranate As Anti-Aging and Anti-
Acne

group V : 3 volunteers were given cream with F4

Data were analyzed using SPSS. Data were analyzed using One Way ANOVA.
2.9.2 Microbiology Testing Anti-acne
Weighed as much as 1 g of cream of each formula, put in vials and labeled then
added 1 ml sterile distilled water and stirred until dissolved. 0.1 ml inoculum put into a
sterile petri dish, then poured nutrient agar medium 20 ml at a temperature of 45-50o C.
Subsequently the cup rocked on the surface of the table, so that the media and the
bacterial suspension well blended. Pencadang paper that has been soaked in the test
solution is placed on the surface of the cream that has a solid medium, then incubated in
an incubator at a temperature of 35 ± 2 ° C for 18-24 hours, after which the measured
diameter bottleneck area (clear zone) growth of around pencadang using calipers.
3. RESULTS AND DISCUSSION
3.1 Sample Identification Results
The results of sample identification is done Herbarium Medanense Plant
Taxonomy Laboratory of the Department of Biology, University of Medan, North
Sumatra of the pomegranate plant is a type of Punica granatum L with tribal
Punicaceae and betel plant is Piper betle L with Piperaceae tribe. Commented [u33]: satukan dengan metode

3.2 Characterization Inspection Results

The results of macroscopic examination simplicia betel leaf that leaves are oval
to oblong, pointed tip, base heart-shaped, slightly curved, 7-13 cm long and 5-10 cm
wide. Brownish-green color, lower surface rough, dull, colorless younger than the top
surface. Typical smell and spicy taste. The results of macroscopic examination of
pomegranate leaf botanicals that single short-stemmed leaves, shaped lonjongsampai
Lanse, base tapering, blunt tip, flat edge, pertulangan pinnate, shiny surface, 1-9 cm
long and 0.5-2.5 cm wide green.
Table 2 Characterization Examination Results Betel and Pomegranate Leaf
No. Testing parameters Results Results Terms FHI
obtained obtained
1 Determination of total 3.33% 1.977% ≤ 3.7%
ash
 Formulation KRISI5:
Cream Of The combination of Betel Leaf And Leaves Pomegranate As Anti-Aging and Anti-
Acne

2 Determination of acid 1% 2.54% ≤ 1.1%

insoluble ash content
3 Determination of 9.67% 9.56% ≤ 10%
water content
4 Assay of the water 32% 23% ≤ 14.4%
soluble extract
5 Assay of ethanol 20.67% 13% ≥ 8.1%
soluble extract

3.3 Extraction Results

500 g of crude drug extraction results pomegranate leaves by maceration using a solvent
96% ethanol is derived ethanol extract of pomegranate leaf as much as 100 g and 500 g
of crude drug extraction results betel leaf maceration using ethanol 96% ethanol extracts
Obtained as much as 100 g of betel leaf,
3.4 Screening results Phytochemicals
Table 3. Results of phytochemical screening betel leaf
Pomegranate Leaf
No. Parameter Betel Leaf Extract
Extract
1. Alkaloids Positive Positive
2. Flavonoids Positive Positive
3. Saponin Positive Positive
4. Tanin Positive Positive
5. Triterpenoids /
Positive Negative
steroids

3.5 Results Determination of Physical Quality preparations Cream

3.5.1 Homogeneity Examination Results
The preparation showed a homogeneous composition and no visible coarse grained.
 Formulation KRISI5:
Cream Of The combination of Betel Leaf And Leaves Pomegranate As Anti-Aging and Anti-
Acne

Figure 1. Homogeneity cream F0

Figure 2. Homogeneity cream F1

Figure 3. Homogeneity cream F2

Figure 4. Homogeneity cream F3

 Formulation KRISI5:
Cream Of The combination of Betel Leaf And Leaves Pomegranate As Anti-Aging and Anti-
Acne

Figure 5. Homogeneity cream F4

3.5.2 Results of pH Preparations

Table 4. Results of pH Preparations Every Formula
F0 F1 F2 F3 F4
6.0 5.8 6.1 6.0 6.2

3.5.3 The results of emulsion type preparations

Based on the results of tests performed, that the anti-aging cream from a
combination of ethanol extract of pomegranate leaf and betel leaves have the type
of emulsion O / W (Oil in water) as methylene blue can be dissolved and provide a
homogeneous color that can be seen in Table 4.2 below:
Table 5. The test results cream emulsion type dosage forms, F1, F2, F3, F4 by
using methylene blue staining.
The solubility of methylene blue in the preparation
No. Cream
Yes No
1 A √ -
2 B √ -
3 C √ -
4 D √ -
5 E √ -
Information : Cream A: Blanko (without extract)
Cream B: Formula 1
 Formulation KRISI5:
Cream Of The combination of Betel Leaf And Leaves Pomegranate As Anti-Aging and Anti-
Acne

Cream C: Formula 2
Cream D: Formula 3
Cream E: Formula 4
3.5.4 Evaluation of the stability of the preparations
Evaluation of the stability of the preparation is done during 4 weeks of storage,
preparation of cream stored at room temperature and observed changes in odor, color
and rupture of the emulsion. The results of the evaluation of the stability of each
parameter can be seen in:

Table 6. The results of the evaluation of the stability of the preparation

(changes in odor and color) cream form, F1, F2, F3, and F4 on initial

observation After

finished
No.
1 Cream
A - - - 1- week
- - -2 weeks
- - -3 weeks
- - - 4 weeks
- -
2 B - be made
- - - - - - - - - - - - - -

3 C x- y- z - x- y- z- x- y- z- x- y- z- x- y- z-

4 D - - - - - - - - - - - - - - -

5 E - - - - - - - - - - - - - - -
Commented [u34]: perbaiki
Commented [u35]: mana hasil uji klinisnya?
observations and observations on storage for 4 weeks.

Information : Cream A: Blanko (without extract)

Cream B: F1
Cream C: F2
Cream D: F3
 Formulation KRISI5:
Cream Of The combination of Betel Leaf And Leaves Pomegranate As Anti-Aging and Anti-
Acne

Cream E: F4
x: Discoloration
y: Change of odor
z: Rupture emulsion
-: No changes
√: There is a change

3.5.5 Irritation Test Results Against Volunteers

Irritation test carried out on 5 volunteers to determine whether the
preparations are made can cause erythema and edema.
Table 7. The test results irritation to the skin cream preparation volunteers

Volunteer
Irritation
reactions
I II III IV V VI VII VIII IX X

Irritation 0 0 0 0 0 0 0 0 0 0

Itchy 0 0 0 0 0 0 0 0 0 0

Rude 0 0 0 0 0 0 0 0 0 0

Primary irritation index: 0/24 = 0.00

Description: The system scores the Federal Hazardous Substance Act (Barel et al.,
2009).
no 0
the least 1
a little 2
moderate 3
awfully 4
 Formulation KRISI5:
Cream Of The combination of Betel Leaf And Leaves Pomegranate As Anti-Aging and Anti-
Acne

3.6 Diameter Testing Results inhibitory effect on the activity of anti-acne

preparations.
Table 4. Results of Testing Diameter drag on the preparation of antibacterial
activity
Parameter Propionibacterium acne
Blanko 0 mm
F1 17,90
F2 19,90
F3 20,50
F4 21,30 Commented [u39]: sesuaikan penulisan tabel dan pisahkan dari
artikel

4.7 Testing Results Anti-aging

1. Water content (moisture)
Commented [u40]: apa itu blanko
Water Content (Moisture) Commented [u41]: Tidak perlu pakai judul lagi

180 Commented [u42]: Time (week)

160 Commented [u43]: week
140 Commented [u44]: satuannya apa?
Water Content

120
F4
100
80 F3
60 F2
40
20 F1
0 Blanko
Kondisi Minggu 1 Minggu 2 Minggu 3 Minggu 4
awal
Time

Figure 1. Graph the results of measurements of water content (moisture) in facial skin
form a group of volunteers, the cream of pomegranate leaf extract and Daub betel for 4
weeks
Commented [u45]: Tidak perlu pakai judul
Persentasion of water content Sesuaikan dengan gambar di atas

10
water content (%)

F4
5 F3
F2
0
Blanko F1 F2 F3 F4 F1

Formula Blanko
 Formulation KRISI5:
Cream Of The combination of Betel Leaf And Leaves Pomegranate As Anti-Aging and Anti-
Acne

Figure 2. Graph recovery percentage of water content (moisture) in skin form a group
of volunteers, pomegranate leaf extract cream and betel leaf for 4 weeks.
2. Evenness

Evenness
45
40
35
30
Evenness

Blanko
25
20 F1
15
F2
10
5 F3
0
F4
Kondisi minggu 1 minggu 2 minggu 3 minggu 4
awal
Figure 3. Fineness measurement Time
result graph (Evenness) on the skin form a group of
volunteers, pomegranate leaf extract cream and betel leaf for 4 weeks for 4 weeks.

Persentasion of evenness
25
20
Evenness (%)

Blanko
15
F1
10
F2
5
0 F3
Blanko F1 F2 F3 F4 F4
Formula
Figure 4. Graph recovery percentage of water content (moisture) in facial skin form a
group of volunteers, pomegranate leaf extract cream and betel leaf for 4 weeks
3. Pore

Pore
35

30

25

20 Blanko
pore

F1
15
F2
10
F3
5 F4
0
Kondisi awal Minggu 1 Minggu 2 Minggu 3 Minggu 4
time
 Formulation KRISI5:
Cream Of The combination of Betel Leaf And Leaves Pomegranate As Anti-Aging and Anti-
Acne

Figure 5. Graph pore on the skin form a group of volunteers, pomegranate leaf
extract cream and betel leaf for 4 weeks.

Persentasion of pore
70
60
50
Blanko
pore (%)

40
F1
30
F2
20
F3
10
0 F4
Blanko F1 F2 F3 F4
Figure 6. Graph recovery percentage
Formulapore on the skin form a group of volunteers,
pomegranate leaf extract cream and betel leaf for 4 weeks.

Spot
50
45
40
35
30 Blanko
Spot

25
20 F1
15 F2
10
5 F3
0
F4
Kondisi Minggu 1 Minggu 2 Minggu 3 Minggu 4
awal
Figure 7.Graph the results of measurement spots on the skin form a group of
Time
volunteers, cream extract, pomegranate leaf extract cream and betel leaf for 4 weeks.

Persentasion of spot
60
50
40 Blanko
Spot (%)

30 F1
20 F2
10 F3
0 F4
Blanko F1 F2 F3 F4
Formula
 Formulation KRISI5:
Cream Of The combination of Betel Leaf And Leaves Pomegranate As Anti-Aging and Anti-
Acne

Figure 8. Graph recovery percentage spots on the skin form a group of volunteers,
pomegranate leaf extract cream and betel leaves after 4 weeks.

45
Wrinkle
40
35
30
Blank
Wrinkle

25 o
20 F1
15
10 F2
5
0
Figure 9. Kondisi
Graph the results
Minggu of measurements
1 Minggu 2 Minggu 3 of wrinkles
Minggu 4 at the group of volunteers
awal Time cream and betel leaf sselama 4 weeks.
face blank, pomegranate leaf extract

Persentasion of wrinkle
45
40
35
Wrinkle (%)

30 Blanko
25
F1
20
15 F2
10 F3
5
F4
Figure0 10. Graph recovery percentage wrinkles on the skin form a group of
Blanko F1 F2 F3 F4
volunteers, pomegranate leaf extract cream and betel leaves after 4 weeks.
Formula
4. CONCLUSION
 Formulation KRISI5:
Cream Of The combination of Betel Leaf And Leaves Pomegranate As Anti-Aging and Anti-
Acne

After 4 weeks of using a combination cream of pomegranate and betel leaf

ethanol extract, it was able to show its effectiveness as an anti-aging with increased
skin moisture content, smoother skin, smaller pores, diminished stains, wrinkles and
reduced wrinkles depth. combination of pomegranate leaves and betel leaf ethanol
extract has an-acne activity against Propionibacterium acne, with inhibition of 17.90
mm; 19,90 mm; 20.50 mm; 21.30 mm. Cream from a combination of pomegranate
and betel leaf ethanol extract fulfills the requirements of a homogeneous cream
preparation formulation, suitable pH, stable and does not irritate the skin.

5. ACKNOWLEDGMENTS Commented [u46]: Revise sentence. Terima kasih cukup untuk

kemenristek dikti untuk ibu tidak perlu dimasukkan hehe
Researchers say thank you profusely at the Ministry of Research, Technology
and Higher Education of the Republic of Indonesia on funding, as well as the University
of North Sumatra and lecturer Marianne companion, S.Si., M.Sc., Apt. for all their help
and motivation so that this research can be accomplished.

6. REFERENCES Commented [u47]: Sedapat mungkin refference dari luar

negeri
Amjad, L., & Shafighi, M. 2012. Antioxidant activity of leaf different extracts in Punica Commented [u48]:

granatum. Int J Biol Med Res. 3(3): 2065 - 2067

Ansel, H.C. 1989. Pengantar Bentuk Sediaan Farmasi. Edisi ke-4, Jakarta: UI Press.
Halaman: 156-158, 513.
Ardhie, M.A. 2011. Radikal Bebas dan Peran Antioksidan dalam Mencegah Penuaan.
Medicinus. 24(1): 5-9.
Dalimartha, S. 2009. Atlas Tumbuhan Obat Indonesia. Jilid 6. Jakarta: Pustaka Bunda.
Halaman: 157.
Depkes, RI. 1995. Farmakope Indonesia. Edisi Keempat. Jakarta: Depkes RI. Halaman:
6.
Draelos, Z.D., dan Thaman, L.A. 2006. Cosmetic Formulstion of Skin CareProduct.
Volume 30. New York: Taylor and Francis Group. Halaman. 167,174.
Faralia. 2012. 1001 Khasiat Istimewa Buah-Buahan dan Sayuran. Yogyakarta: Aulia
Publishing.: 32-36.
 Formulation KRISI5:
Cream Of The combination of Betel Leaf And Leaves Pomegranate As Anti-Aging and Anti-
Acne

Hariana HA. 2008. Tumbuhan Obat dan Khasiatnya. Jakarta: Penebar Swadaya.
Halaman: 106-107.
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