Somnath Dutta E-mail: duttasomnath43@gmail.

com

Objectives
Skills

Microscopy:

• Use of confocal microscopy in cell biology

• Live cell imaging of Saccharomyces cerevisiae
• Transmission electron microscopy (TEM)
• Immunofluorescence
Molecular biology:

• Molecular cloning, site-directed mutagenesis and recombinant DNA

technology
• Bacterial competent cell preparation, transformation, plasmid DNA
isolation
• RNA isolation
• PCR, real-time quantitative PCR, DNA sequencing
• Agarose gel electrophoresis
• Yeast gene deletion by homologous recombination
Biochemistry:

• SDS-PAGE, western blot

• Immunostaining
• Protein expression and purification
• Chromatography techniques
Biological system:

• Maintenance of Giardia lamblia cell culture

• Animal handling (Rabbit and mice)
• Mammalian cell culture
Image analysis:

• Processing of image and image analysis using Adobe Photoshop, Auto

quant
Microbiology:

• Determination of quality of water

• Isolation of microbes from natural sources
 • Antibiotic assay, MIC
• Plaque assay

Computational skills:

• HTML, CSS
• OS-Windows
• Microsoft office
• Vector NTI
• Various in silico packages like PyMol, PDB, ExPassy, BLAST, Clustal W,
Jalview

EDUCATION
Research Associate
(Oct 2016-present)
Ph.D.:
(Jan 2010-Sep 201
Master of Sciences:
Biotechnology,
(Sep 2006-Aug 2008)
Bachelor of Sciences:
Microbiology (Honours)
(July 2003-June 2006)
Structural analysis of Nuclear Pore Complex
Ph.D. degree awarded on 25th October 2016. Bose Institute, Kolka, India
Broad research area: Parasitology/Cell Biology
Thesis title: “In search of putative ESCRT components in Giardia lamblia”
Affiliated to the Department of Microbiology, University of Calcutta
India
An interdisciplinary course. Secured first class. The University
of Burdwan, Burdwan, India.
With Chemistry and Physics as minor. Secured first
class. University of Calcutta, Kolkata, INDIA

RESEARCH EXPERIENCE
Oct 2016-Present Research Associate at National Centre for Cell Science, Pune, INDIA

Sep 2011- Sep 2016 Research fellow and Ph.D. student at Bose Institute Kolkata, INDIA
 Thesis supervisor Dr. Srimonti Sarkar, Principal Scientist, Department of
Biochemistry
Jan 2010-Aug 2011 Research fellow and Ph.D. student at Indian Institute of Science Research
and Education, Kolkata, INDIA. Thesis supervisor Dr. Srimonti Sarkar,
Principal Scientist, Department of Biological Sciences
June 2007-July 2007 Summer Research Fellow at Virology Division, National Institute of
Cholera and Enteric Diseases, Kolkata, INDIA

AWARDS and ACHIEVEMENTS

October 2017 Project proposal sanctioned by DST-SERB for National Post-Doctoral Fellowship

June 2009 Qualified CSIR-UGC Junior Research fellowship through All India National
Eligibility Test, securing All India Rank 81
2006 Qualified Selection Examination for Master of Science in Biotechnology
at The University of Burdwan (Affiliated to DBT, JNU), at Burdwan, INDIA

Publication

Research article Dutta, Somnath, Nabanita Saha, Atrayee Ray, and Srimonti Sarkar.
"Significantly diverged Did2/Vps46 orthologues from the
protozoan parasite Giardia lamblia." Current microbiology71, no. 3
(2015): 333-340.

Saha, Nabanita, Somnath Dutta, Shankari P. Datta, and Srimonti

Sarkar. "The minimal ESCRT machinery of Giardia lamblia has
altered inter-subunit interactions within the ESCRT-II and ESCRT-III
complexes." European journal of cell biology 97, no. 1 (2018): 44-62.
 CONFERENCE PROCEEDINGS/WORKSHOP

October 2015 DST sponsored National Seminar on New Horizons In Biotechnology

(India), Haldia
Oral presentation: “In search of ESCRT complexes in a protozoan

parasite Giardia lamblia” . Somnath Dutta and Srimonti Sarkar

January 2015 BIOTICA 2015, Kolkata An International symposium on current
perspective in Life Sciences
December 2014 83rd Annual Meeting of Society of Biological Chemists (India) Bhubaneswar
Oral presentation: “In search of putative ESCRT components in Giardia
lamblia”. Somnath Dutta and Srimonti Sarkar
December 2014 83rd Annual Meeting of Society of Biological Chemists (India), Bhubaneswar
Poster presentation: “In search of putative ESCRT components in Giardia
lamblia”. Somnath Dutta and Srimonti Sarkar
November 2013 54th Association of Microbiologists Meeting (India), Rohtak
Poster presentation: “Understanding the role of ESCRT complexes in a
Protozoan parasite Giardia lamblia”. Somnath Dutta and Srimonti Sarkar
November 2012 81st Annual Meeting of Society of Biological Chemists (India) Kolkata
Poster presentation: “In search of putative ESCRT-like components in an
early diverged protist Giardia lamblia”. Somnath Dutta and Srimonti
Sarkar
December 2009 International Conference on Plant Systems Biology (India) Kolkata

References

Dr. Srimonti Sarkar Dr. Manju Roy Dr. Pranab Roy

Professor Honorary Visiting Professor, Professor
Department of Biochemistry Department of Biophysics Department of Biotechnology
Bose Institute Bose Institute Haldia Institute of Technology
Centenary Campus Centenary Campus I.C.A.R.E Complex, H.I.T Campus
P 1/12 CIT Scheme VIIM P 1/12 CIT Scheme VIIM Hatiberia, PO-HIT
Kolkata 700054 Kolkata 700054 Dist: Midnapore (E), West Bengal,
West Bengal, India. West Bengal, India. India.
Contact: +91-33-25693229 Contact: 033-2569 3334 PIN : 721657
Email: srimontisarkar@jcbose.ac.in Email: manjuray@jcbose.ac.in Contact: +91-9933037099
Email: pranabroy@rediffmail.com