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y=mx+b
Absorbancia =m (concentración) + b
y=144.0027x + 0.0207
𝑦−0.0207
x= 144.0027
0.5
Absorbancia
0.4
0.3
0.2
0.1
0
0 0.0005 0.001 0.0015 0.002 0.0025 0.003 0.0035 0.004 0.0045
Concentración (mg/mL)
Memoria de cálculo.
Concentraciones.
𝑦−0.0207
x= 144.0027
Paranitrofenol libre Paranitrofenol total
- Perejil
0.235−0.0207 mg 0.143−0.0207 mg
X= = 0.0015 mL X= = 0.00085 mL
144.0027 144.0027
- Jitomate
0.02−0.0207 mg 0.017−0.0207 mg
x= = - 0.0000049 mL x= = - 0.000026 mL
144.0027 144.0027
Col
0.141−0.0207 mg 0.097−0.0207 mg
X= = 0.00084 mL X= = 0.00053 mL
144.0027 144.0027
- Lechuga
0.377−0.0207 mg 0.146−0.0207 mg
X= = 0.0025 mL X= = 0.00087 mL
144.0027 144.0027
mg/g de paranitrofenol libre.
- Perejil.
0.0015 mg 50 𝑚𝐿 50 𝑚𝐿 100 𝑚𝐿 mg
( )( )( )( ) = 1.5
1 𝑚𝐿 5 𝑚𝐿 5 𝑚𝐿 10 𝑔 g
- Jitomate
−0.0000049 mg 50 𝑚𝐿 100 𝑚𝐿 mg
( )( )( ) = - 4.9 x 10 -4
1 𝑚𝐿 5 𝑚𝐿 10 𝑔 g
-Col
0.00084 mg 50 𝑚𝐿 100 𝑚𝐿 mg
( )( )( ) = 0.084
1 𝑚𝐿 5 𝑚𝐿 10 𝑔 g
- Lechuga
0.0025 mg 50 𝑚𝐿 100 𝑚𝐿 mg
( )( )( ) = 0.25
1 𝑚𝐿 5 𝑚𝐿 10 𝑔 g
- Jitomate
−0.000026 mg 100 𝑚𝐿 100 𝑚𝐿 mg
( )( )( ) = - 5.2 x 10-3
1 𝑚𝐿 5 𝑚𝐿 10 𝑔 g
-Col
0.0053 mg 100 𝑚𝐿 100 𝑚𝐿 mg
( )( )( ) = 0.106
1 𝑚𝐿 5 𝑚𝐿 10 𝑔 g
- Lechuga
0.00087 mg 100 𝑚𝐿 100 𝑚𝐿 mg
( )( )( ) = 0.174
1 𝑚𝐿 5 𝑚𝐿 10 𝑔 g
mg/g de paratión.
paranitrofenol total − paranitrofenol libre
0.478
- Perejil
𝑚𝑔 𝑚𝑔
1.7 − 1.5
𝑔 𝑔 mg
= 0.418 g
paratión
0.478
-Jitomate
𝑚𝑔 𝑚𝑔
−0.0052 − 0.00049
𝑔 𝑔 mg
= - 0.0099 g
paratión
0.478
- Col
𝑚𝑔 𝑚𝑔
0.106 − 0.084
𝑔 𝑔 mg
= 0.046 g
paratión
0.478
- Lechuga
𝑚𝑔 𝑚𝑔
0.174 − 0.25
𝑔 𝑔 mg
= - 0.159 g
paratión
0.478