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OBJECTIVES
i. To determine the presence of functional group or molecule in olive oil, butter solution,
methanol extract, acetone extract and unknown samples using ester assay, phosphate assay
and cholesterol assay.
ii. To prepare carboxylic acid salt from lipids
iii. To identify the unknown sample
MATERIALS
Extraction of egg yolk Chloroform
Chloroform solution of olive oil (1%) Methylene chloride
Chloroform solution of butter (0.5%) Acetic anhydride
Chloroform solution of X (unknown) Sulphuric acid
Ethanol
2M hydroxylamine hydrochloride
3.5M NaOH
HCL (5M and 1M)
Ferric chloride in 0.1M HCL (5%)
Ammonium Molybdate in 4M nitric acid
(6%)
PROCEDURES
1a. Sample preparation (extraction from egg yolk)
i. An egg was cracked open gently. Do Not Bust The Yolk
ii. The yolk was separated from the white using the shell. The white was discarded.
iii. A glass centrifuge tube was tared. The yolk was added and its mass and volume was
recorded.
iv. The yolk was shaken vigorously to homogenize it for one minute.
v. The yolk was split into 2 equal parts and the following steps were performed.
REFERENCES
Hassan, W. M., Yahya, M. R., & Ariffin, Z. Z. (n.d.). Biochemistry laboratory manual. Universiti
Teknologi MARA.
Lim, C.Y. (n.d.). Biology Unit Labuan Matriculation College. Labuan Matriculation College.
UiTM. (n.d.). Biochemistry notes.