InCCA Bit Service Manual SM160101

SERVICE MANUAL
v160101
InCCA Bit Service Manual - MSv160101

1 TRANSPORTATION, STORAGE AND UNPACKING. ............ 1-1
1.1 TRANSPORTATION AND STORAGE ...................................................................... 1-1

1.2 UNPACKING INSTRUCTIONS ............................................................................. 1-2
2 INSTALLATION AND CONNECTIONS OF THE ANALYZER ... 2-1
2.1 ELECTRICAL REQUIREMENT .............................................................................. 2-1

2.2 DIMENSIONS ............................................................................................... 2-1
2.3 ENVIRONMENTAL REQUIREMENTS ..................................................................... 2-1
2.4 MOVING THE INSTRUMENT.............................................................................. 2-1
2.5 PC MINIMUM REQUIREMENTS.......................................................................... 2-1
2.6 INSTRUMENT PLACEMENT AND PREPARATION ....................................................... 2-2
2.7 WIRES AND TUBINGS CONNECTION ................................................................... 2-3
2.8 WASHING SOLUTION ..................................................................................... 2-5
2.8.1 WASHING SOLUTION FOR PROBE ............................................................................................... 2-5
2.9 INSTALLATION .............................................................................................. 2-5
2.9.1 PC CONFIGURATION ............................................................................................................... 2-5
2.9.2 ANALYZER SETUP ................................................................................................................... 2-7
3 ANALYZER TECHNICAL SPECIFICATIONS .......................... 3-1
3.1 GENERAL SPECIFICATIONS ............................................................................... 3-1

3.2 SAMPLE/REAGENT TRAY ................................................................................ 3-2
3.3 CUVETTES/REACTION TRAY ............................................................................. 3-2
3.4 DISPENSING ARM AND HYDRAULIC SYSTEM......................................................... 3-2
3.5 PHOTOMETER .............................................................................................. 3-3
3.6 SOFTWARE .................................................................................................. 3-3
3.7 POWER REQUIREMENT ................................................................................... 3-3
3.8 DESCRIPTION OF ELECTRONIC CIRCUITS ............................................................... 3-4
3.9 TYPES OF SAMPLES........................................................................................ 3-4
3.10 TYPES OF REACTIONS ................................................................................... 3-4
3.11 TYPES OF METHODS .................................................................................... 3-4
4 GENERAL FUNCTIONS ..................................................... 4-1

4.1 MAIN PARTS OR MODULES .............................................................................. 4-1
4.1.1 SAMPLE/REAGENT TRAY ......................................................................................................... 4-1
4.1.2 DISPENSING ARM AND PROBE .................................................................................................. 4-2
4.1.3 CUVETTE/REACTION TRAY ....................................................................................................... 4-3
4.1.4 HYDRAULIC SYSTEM ............................................................................................................... 4-4
4.1.5 OPTICAL SYSTEM ................................................................................................................... 4-5
4.2 DESCRIPTION OF THE OPERATING CYCLE ............................................................. 4-6
4.2.1 REACTION PREPARATION CYCLE ................................................................................................ 4-6
4.2.2 INCUBATION AND READING CYCLE ............................................................................................. 4-6
5 BASIC OPERATIONS ......................................................... 5-1
5.1 USING COMMANDS CONSOLE .......................................................................... 5-1

5.2 COMMUNICATION PROTOCOL FOR TECHNICAL SERVICE USE ..................................... 5-2
5.2.1 INTRODUCTION ..................................................................................................................... 5-2
5.2.2 MESSAGE STRUCTURE ............................................................................................................ 5-2
5.2.3 COMMANDS DESCRIPTION ....................................................................................................... 5-2
5.2.4 SYNCHRONOUS ANSWER DESCRIPTION ...................................................................................... 5-5
5.2.5 ASYNCHRONOUS ANSWER DESCRIPTION .................................................................................... 5-5
5.3 COMMAND CONSOLE - UNIT QUICK CHECK - ....................................................... 5-8
5.4 EEPROM (ELECTRICALLY ERASABLE PROGRAMMABLE READ-ONLY MEMORY)
VERSION 1.09 ................................................................................................... 5-9
5.5 OBTAINING FIRMWARE PARAMETERS .............................................................. 5-15
6 DIRECTIONS FOR ASSEMBLING AND DISASSEMBLING ..... 6-1
6.1 DISASSEMBLING THE CABINET........................................................................... 6-1

6.2 ROBOT/HEADER MODULE .............................................................................. 6-2
6.3 SAMPLE/REAGENT MODULE ........................................................................... 6-2
6.4 CUVETTE MODULE ........................................................................................ 6-2
6.5 PHOTOMETER MODULE.................................................................................. 6-3
6.6 ELECTRONIC MODULE .................................................................................... 6-4
6.6.1 DILUTER............................................................................................................................... 6-6
6.6.2 SOLENOID VALVE N.O. ........................................................................................................... 6-6
7 MODULES ADJUSTEMENT ............................................... 7-1
7.1 REACTION TRAY DISPENSING OFFSET CALIBRATION ............................................... 7-1

7.2 REACTION TRAY PHOTOMETER OFFSET CALIBRATION ............................................. 7-2
7.3 ADJUST ROBOT ARM REFERENCE POSITION........................................................... 7-3

7.4 HEAD ROBOT POSITION OVER DISPENSE CALIBRATION ........................................... 7-4
7.5 CUVETTES BASE VERTICAL MOVEMENT CALIBRATION ............................................ 7-4
7.6 CUVETTES BASE VERTICAL MOVEMENT DURING MIXING CALIBRATION ....................... 7-5
7.7 HEAD ROBOT POSITION OVER FUNNEL CALIBRATION ............................................. 7-5
7.8 FUNNEL BASE VERTICAL MOVEMENT CALIBRATION ............................................... 7-6
7.9 HEADER ROBOT POSITION OVER REAGENT CALIBRATION ........................................ 7-6
7.10 REAGENT A CONTAINER BASE VERTICAL MOVEMENT CALIBRATION ......................... 7-7
7.11 HEADER ROBOT POSITION OVER SAMPLE TUBE CALIBRATION................................. 7-7
7.12 SAMPLE TUBES BASE VERTICAL MOVEMENT CALIBRATION .................................... 7-8
7.13 WASTE CONTAINER VOLUME CALIBRATION ....................................................... 7-8
7.14 PROBE WASHING SOLUTION CONTAINER VOLUME CALIBRATION ............................ 7-9
8 PARTS REPLACEMENTS ................................................... 8-1
8.1 LIGHT BULB REPLACEMENT .............................................................................. 8-1

8.2 INTERFERENTIAL FILTERS REPLACEMENT .............................................................. 8-3
8.3 SAMPLE / REAGENT PROBE REPLACEMENT ........................................................... 8-4
8.4 CUVETTES REPLACEMENT ................................................................................ 8-8
8.5 FUSES REPLACEMENT ..................................................................................... 8-8
9 MAINTENANCE PROGRAM .............................................. 9-1
9.1 DAILY MAINTENANCE .................................................................................... 9-1

9.1.1 BEFORE STARTING DAILY OPERATION.......................................................................................... 9-1
9.1.2 UPON FINISHING WITH THE DAILY OPERATION .............................................................................. 9-1
9.2 WEEKLY MAINTENANCE ................................................................................. 9-1
9.3 MONTHLY MAINTENANCE............................................................................... 9-1
9.3.1 EXTERNAL WASHING OF CUVETTES ............................................................................................. 9-1
9.3.2 GENERAL WASHING OF THE INSTRUMENT .................................................................................... 9-2
9.3.3 BACK-UP OF FILES IN USE ......................................................................................................... 9-2
9.4 MAINTENANCE BASED ON ALARMS ......................................................... 9-3
9.4.1 ALARMS GENERATED BY COUNTERS ................................................................................ 9-3
9.4.2 ENABLING OF COUNTERS ......................................................................................................... 9-4
9.5 LEVEL 1 MAINTENANCE .................................................................................. 9-5
9.5.1 GENERAL WASHING OF THE INSTRUMENT .................................................................................... 9-5
9.5.2 LUBRICATING THE AXELS .......................................................................................................... 9-6
9.5.3 INITIALIZING THE COUNTER ...................................................................................................... 9-6
9.6 MAINTENANCE ACCORDING TO NEED ................................................................ 9-6
9.6.1 DATABASE INITIALIZATION (EVERY 10.000 DETERMINATIONS) ........................................................ 9-6
9.6.2 LIGHT BULB REPLACEMENT ...................................................................................................... 9-8

9.6.3 CUVETTES REPLACEMENT ........................................................................................................ 9-8
9.6.4 FUSES REPLACEMENT ............................................................................................................. 9-8
10 TROUBLESHOOTING .................................................... 10-1
10.1 CHEMICAL PROBLEMS ................................................................................ 10-1

10.1.1 HIGH RESULTS ................................................................................................................... 10-1
10.1.2 LOW RESULTS ................................................................................................................... 10-2
10.1.3 ERRATIC RESULTS............................................................................................................... 10-3
10.1.4 ONLY ONE AFFECTED SAMPLE FOR ALL OF THE METHODS ............................................................ 10-5
10.1.5 ONLY ONE AFFECTED METHOD FOR ALL OF THE SAMPLES ............................................................ 10-5
10.2 INSTRUMENTAL PROBLEMS.......................................................................... 10-6
10.2.1 CONNECTIONS AND SUPPLIES ............................................................................................... 10-6
10.2.2 HYDRAULIC SYSTEM ............................................................................................................ 10-7
10.2.3 DILUTER........................................................................................................................... 10-7
10.2.4 SAMPLE/REAGENT PROBE .................................................................................................... 10-8
10.2.5 REACTION CUVETTES PLATE ................................................................................................. 10-9
10.2.6 OPTICAL SYSTEM................................................................................................................ 10-9
10.2.7 SOFTWARE ....................................................................................................................... 10-9
10.3 MALFUNCTION PROBLEMS DISPLAYED ON THE SCREEN .......................................10-10
10.3.1 GENERAL ..................................................................................................................... 10-11
10.3.2 EEPROM ...................................................................................................................... 10-11
10.3.3 VERTICAL..................................................................................................................... 10-12
10.3.4 HORIZONTAL ............................................................................................................... 10-15
10.3.5 REACTION TRAY ............................................................................................................... 10-17
10.3.6 FILTER WHEEL ............................................................................................................. 10-20
10.3.7 READING (AMPLIFIERS)............................................................................................... 10-22
10.3.8 PHOTOMETER CALIBRATION ...................................................................................... 10-25
10.3.9 CUVETTES CALIBRATION. ................................................................................................... 10-28
10.3.10 HEATER AND PRE-HEATER ....................................................................................... 10-30
10.3.11 DILUTER .................................................................................................................... 10-30
10.4 LOG FILE ................................................................................................10-31
11 VALIDATIONS .............................................................. 11-1
11.1 STRAY LIGHT............................................................................................ 11-1

11.2 PHOTOMETER PRECISION ............................................................................ 11-2
11.3 PHOTOMETRIC ACCURACY........................................................................... 11-3
11.4 PHOTOMETRIC LINEARITY............................................................................ 11-5
11.5 DILUTER PRECISION ................................................................................... 11-6
12 DIAGRAMS .................................................................. 12-1

12.1 IDENTIFYING PRINTED CIRCUITS ..................................................................... 12-1
12.2 ELECTRICAL AND ELECTRONIC SCHEME ............................................................ 12-2
12.3 MECHANICAL SCHEME ................................................................................ 12-1
12.3.1 ROBOT HEADER MODULE ............................................................................................. 12-1
12.3.2 ROBOT MODULE ........................................................................................................... 12-2
12.3.3 CUVETTES TRAY MODULE ............................................................................................. 12-2
12.3.4 PHOTOMETER MODULE ............................................................................................... 12-3

INTENDED USE OF THE ANALYZER
InCCA Bit is a photometric analyzer for clinical chemistry, it is meant to perform automated
analytic procedures for “in vitro” diagnosis.
TERMS OF USE
1. The instrument must be installed by trained personnel.
2. This instrument is Class 1, Type b, IPX0.
3. The instrument must be used by trained personnel
4. Operation room requirements: Room temperature 20-25ºC; Humidity: 20- 85% (no
condensation).
5. The instrument must be connected to a supply line according to current national regulations.
6. This instrument must not be used for purposes other than the ones it has been built for.
7. After switching on the instrument, wait 10 minutes before beginning analysis.
8. After powering off, wait at least 20 seconds before restarting.
9. If line variations are higher than 10%, a ferroresonant voltage regulator or a 1500 VA
uninterruptible power supply (UPS) is recommended.
10. Read the entire User’s Manual before using the instrument
11. The laboratory must count on a special residues collection service, to handle wastes.
12. Do not discard the analyzer, neither its accesories, along with domestic residues.Check the
local terms for its correct elimination. Is the user’s responsability to deliver the analyzer in
the indicated pickup point for recycling of electric and electronic devices, otherwise get in
contact with Diconex or its authorized agent to proceed to its removal in a safe and
ecological way.
13. Use only expendables and spare parts provided by Diconex or its authorized agent.
14. If any failure occurs, contact your local authorized technical service, or contact Diconex:
Torcuato de Alvear 46
B1878DMB - Quilmes - Buenos Aires
Argentina
Tel/Fax +54 11 4252 - 2626
www.diconex.com

PRECAUTIONS
Electrical Risks
Do not open those compartments marked as Electrical Risk, neither wires if deteriorated.
Mechanical Risks
Keep lids closed when the instrument is working.

Do not touch mechanical arms or other moving parts while the instrument is working.
Do not wear rings, bracelets, necklaces, etc. as they may get caught in operating mechanical systems.
Do not try to change or touch sample tubes, reagents, etc. when the instrument is running to prevent
breakage and/or an accident when the Reagent Sample Tray moves.
Make sure that the instrument is paused to add reagents, samples, etc.
Chemical risks
Always wear protective apparel when operating the analyzer (gloves and safety glasses).
Follow specific recommendations on the bottles of each reagent or washing solution.
Read safety information of materials provided by manufacturers to be aware of possible danger and to
learn how to prevent it.
In case of handling toxic reagents, follow the manipulation instructions given by the reagent
manufacturer.
Biological risks
Rings and long nails can easily break gloves and increase exposure to biological risks.
Always treat samples and reagents as potentially infectious.
Waste and waste deposits must be treated as toxic and biologically hazardous. Handle them and
eliminate them in accordance with routine laboratory protocols.
Follow in force standards given by local sanitary authority.
Eye risks
Do not look straight at barcode detector light beams.

Do not look straight at the light source of spectrophotometers since they can emit ultraviolet rays.

WARNINGS ABOUT THE INSTRUMENT AND
LABORATORY PRACTICES
The use of calibrators is recommended; refer to reagent manufacturer instructions for specific
calibration instructions.
The use of controls, as indicated by good laboratory practices, is also recommended.
Carry out the maintenance plan as indicated in this manual.
Read all warning messages.
Results may or may not be validated by the user depending on the validation criteria.
Symbols used in the manual and the instrument
Warning
Ground Connection
Manufacturing Date
Consult instructions for use
“In vitro” diagnostics device
Biological risk
Serial number
Fuse

Warranty Terms
Diconex S.A. warrants the instrument for a period of 12 months as of the date the instrument leaves
the factory. This warranty is only effective as long as the pertinent warranty card together with the
installation record with all the data is duly completed and sent to us by certified mail.
Warning: This warranty only covers defective materials or manufacturing defects, according to
examination carried out in the factory and shall be limited to the replacement of defective materials.
Accessories which are not provided by Diconex S.A., such as: wash solutions, standards, controls,
reagents and consumables are not covered by the warranty. Furthermore, costs arising from
mishandling the instrument and/or damage to the instrument are not covered by this warranty.
This warranty shall not be valid if personnel not authorized by Diconex S.A. attempt to repair the
instrument.
WARRANTY SERVICE: Free of charge at Diconex S.A. Otherwise, transport and traveling expenses of a
technician to the place where the instrument is to be repaired shall be paid. Such costs shall not be
born by Diconex S.A.
DICONEX S.A. RESERVES THE RIGHT TO MODIFY THE INSTRUMENTS WITHOUT AFFECTING THEIR
OPERATING PARAMETERS.
NOTE: The following elements are not covered by warranty terms: halogen lamps, fuses, cuvettes,
reagents bottles, tubes and tubings.

1 TRANSPORTATION, STORAGE AND UNPACKING.
Labels indicating this operation are found on each side of the packaging box.
Figure 1-1
1.1 Transportation and storage
Do not pile up more than four wooden boxes
Keep dry, protect from humidity and rain
Fragile
Handle with care
Store at 0 C - 50 C
Gross mass 67 Kg
Move using an automatic or a manual lift

1-1
1.2 Unpacking instructions
Warning: Unpacking shall be carried out by trained personnel. Carefully unpack the
instrument to prevent damage.
Remove the wooden box lid by unscrewing the eight screws located in the front, back and sides.
Figure 1-2
NOTE: Instrument remains fastened to the bottom.
Remove the instrument from the bottom of the box as shown in Figure 1-3 and as explained below.
On the upper part of the instrument you will find an Allen wrench Nº 6, which will be used to remove
the screws joining the bottom to the instrument.

1-2
Figure 1-3
Unscrew the four screws located on the sides.
Screws are placed this way:
Figure 1-4
Once the screws have been removed and the instrument is released, the protective Styrofoam is
removed and the instrument can be placed on the working surface.

1-3
2 INSTALLATION AND CONNECTIONS OF THE ANALYZER
Installation must be carried out by trained personnel.
2.1 Electrical requirement
A standard 110 Volts 60 Hz or 220 Volts 50 Hz socket for 400 VA consumption. The supply line has a
third terminal to ensure proper ground connection. If line variations are higher than 10%, a
ferroresonant voltage regulator, or a 1500 VA uninterruptible power supply (UPS sine wave output) is
recommended.
2.2 Dimensions
Width: 80 cm, Height: 47 cm, Depth: 58 cm
2.3 Environmental requirements

Room temperature: 20 – 25 °C
Humidity: 20 – 85% (no condensation)
2.4 Moving the instrument

Avoid hitting the instrument as well as subjecting it to any kind of vibration while moving it.
2.5 PC minimum requirements

The PC must have the following:
-2 GHz Pentium IV
-RAM Memory, 512 MB
-Independent video card
-40 GB Hard Drive
-CD Rom
-CD Reader
-Windows XP
-Printer (optional)
The PC must comply with safety regulations IEC 60950 and have a certified source.

2-1
2.6 Instrument placement and preparation
Warning: Avoid hitting the instrument as well as subjecting it to any kind of vibration while
moving it.
The instrument must be placed on a counter in a room free from dust and corrosive vapors to ensure
its proper operation.
The instrument must not be subject to vibrations or sudden changes of temperature.
Leave a 20 centimeter separation between the equipment and the wall to ensure proper ventilation.
Liquid containers must be under the counter. Avoid collapsing and/or curving of waste exit tubing.
Avoid any centrifuge, lifts or x-ray supply lines or any other kind of noise-generating equipment in
the supply line.
Avoid direct sun light or illumination on the instrument.
The supply line has a third ground conductor for protection purposes.
Connect the instrument to the computer and its peripherals before connecting to the supply line.
If the above requirements are not met, the quality of results may be affected.
Warning: Make sure that ground connection in the line meets the standard requirements for
its operating power. Not performing ground connection poses significant safety risk for the
operator and may damage one or several parts of the equipment or the computer.
Warning: Waste solution tubings must be placed correctly so that it drains by gravity. They
must not be curved and/or collapsed.
Warning: The instrument must be connected to a PC which meets the requirements

previously mentioned.
Warning: The instrument has been tested with a tensioactive solution which is added to the
distilled water. Not using or altering the product or its dilution will affect the operation of the
instrument.

2-2
2.7 Wires and Tubings Connection
Tubbings connections
Figure 2-1
Switch On/OFF RS 232 Serial Port

Interlock Connector
1. Connect the interlock to 110-220 V, 50-60 Hz supply.
2. If line variations are higher than 10%, a ferroresonant voltage regulator or a 1500 VA
uninterruptible power supply (UPS sine wave output) is recommended.
3. Connect the RS-232 wire to the PC before switching on the instrument.
4. Connect tubings to the appropriate containers according to the indication on the back of the
analyzer as show in the following picture and scheme. (Fig 2-2 – Fig 2-3 )

2-3
Probe Container Connector Waste Container Connector
Pass the tubbings through Pass the tubbings through the

the threaded cap (1) then threaded cap (1) connect the
pass them through the holes transparent tubbing to the piece
in piece (2) and finally (2) , finally pass the yellow
through piece (3) to connect tubing (volume sensor)
Filters and terminal level through the holes in pieces (2)
sensor. and (3) and connect the
terminal level sensor (4).
Figure 2-2
Figure 2-3
Warning: The tubing with the biggest diameter (waste funnel) must be kept straight
downwards without curves to allow free drain of fluids.

2-4
2.8 Washing solution
Warning: Containers must be placed under the analyzer, never on the

same analyzer level.
2.8.1 Washing solution for probe
First wash concentrate solution has to be prepared from Triton X-100 Solution. Then, use wash
concentrate solution to prepare washing solution for probe.
Wash Concentrate: dilute 1 part v/v of Triton X-100 in 9 parts of distilled water, ex: 100 ml Triton X-
100 in 900 ml of distilled water.
The Triton X-100 is extremely viscous. It is suggested to pour the desired quantity into a graduated
cylinder and gradually add the water. Heat the distilled water to 70 – 80 ºC to help dissolve the Triton
X-100.
Washing solution for probe: dilute 7 ml of wash concentrate in 1 liter of distilled water. Ex: 140 ml of
wash concentrate per 20 liters of distilled water.
Warning: not following the instructions given above will affect the results.
2.9 Installation
2.9.1 PC configuration
Open Control Panel  Display  Settings  into Screen Resolution field set 1024x768 pixels
Control Panel  Date and Time  Internet Time  unselect Automatically synchronize with an
Internet time server
Uninstall antivirus
Uninstall Scren Saver

2-5
2.9.1.1 Install analyzer software.
1.Open “Computer”.
2.Find the device where the software installer is.
3.Double Click on the analyzer software installer executable.
4.Press “Next” to continue with the installation or “Cancel” to exit.
5.Choose between “Everyone or Just Me” option (see NOTE).
6.Press “Next” to continue with the installation or “Cancel” to exit. In this step the file folder path
can be changed.
7.Press “Next” to start installation process.
8. Press “Close” to close the window.
NOTE: “Everyone” option means that the software will be installed in all the user accounts existing at
the moment of installation. “Just Me” option means that the software will be installed in the account
that the installer is being executed (if a new account is created then the analyzer software must be
installed in the new account)
If “Just Me” option is chosen the other users can not use analyzer software, unless they install the
analyzer software too, but they have to change the path of all the files when the software is being
installed.
Maintenance Settings  Files tab: all path must be changed for a different location in this user
account.
9.Open analyzer software from desktop.

2-6
2.9.2 Analyzer setup
1.Place the cuvettes in the reaction tray.
Warning: Do not touch the external surface of the cuvette.
Warning: avoid dropping the metal nuts into the interior of the instrument during this
procedure.
2. Turn on the instrument by the General Power button in the back of the analyzer, and then by
pressing the Power button in the top panel from the analyzer (red button) The button in the
middle of the top panel turns on/off the reagents cooling system. (Reagents cooling system is
optional).
 Let the instrument warm up for ten minutes.
General Power
Figure 2-4
3. Run several diluter purge cycles, visually checking that there are no bubbles present
in the diluter’s body (Maintenance  Instrument  Diluter Purge)
4. Calibrate the photometer and all the cuvettes (Maintenance  Instrument  Calibration
 select Calibrate photometer  select Calibrate cuvettes  Range 1 to 100  select the position
of the solution to use ex: Use container  OK)
5. Instrument is ready to use.

2-7
3 ANALYZER TECHNICAL SPECIFICATIONS
Figure 3-1
3.1 General specifications
IEC 61010 Classification: Clase 1, IPX0

IEC 61326 Classification: Clase B
Average throughput with ISE: 250 test/hour
Average throughput without ISE: 120 test/hour
Automatic: Sample and reagents are taken and dispensed without the user's intervention. This also
applies to the reading of reactions.
Random access: Processing order is determined by the user and may be interrupted to allow the
input of stats.

3-1
Discrete: The reaction takes place in the same cuvette in which the reading will be performed. The
use of separate cuvettes for every reaction reduces crossover contamination among samples. The
Analyzer has 100 cuvettes for the reactions and readings to be carried out. Cuvettes are continuously
and automatically washed by the instrument while it is running, as required by dispensing.
Photometric: The Analyzer uses 10 optical filters. An eleventh filter may be added.
3.2 Sample/Reagent Tray

Number of positions for reagent bottles: 24 positions.
Volume of bottles: 32 ml.
Number of positions for samples: Physical capacity for 22 tubes or sample cups.
Positions become available and new samples can be added after the readings of the programmed
methods have been completed.
12 x 75mm primary tubes, test tubes and/or sample cups may be used.
*Refrigeration of reagent tray is operated independently from main power switch. (Optional)
Bar-code ID samples reader: Optional
3.3 Cuvettes/Reaction Tray

Number of cuvettes: 100 PMMA cuvettes placed in 10 different segments.
Reaction volume: minimum reading volume: 220 µl; maximum reaction volume (physical capacity of
the cuvette): 600 µl
Incubation: warm air thermal incubation
Working temperature: 37ºC
The analyzer is prepared to work with up to two cuvettes trays, it detects which one is being used
and automatically loads its calibration.
3.4 Dispensing Arm and Hydraulic System

Samples and reagents preheated to 37ºC.
High precision diluter
Maximum aspiration volume: 500 µl (physical capacity of the diluter)
Internal and external washing of the probe through liquid diaphragm pump
Volume detection
Probe impact sensors

3-2
3.5 Photometer
Interferential filters: 340, 380, 405, 450, 505, 546, 578, 600, 650 and 700 nm and an extra filter as
optional
Krypton-halogen lamp: 12 volts 20 watts
Optical path: 6 mm
Photometric range: -0.100 to 3.600 A.
Type of measurement: monochromatic and bichromatic.
3.6 Software
Easy access menu, with buttons and icons.
Continuous loading of patients, calibrators, controls and reagents during work sequence.
Stat samples
Unlimited number of methods in memory
Statistics for controls, calibrators and patients
Levy-Jennings plots
Linear and non linear multipoint calibration
Interpolation and adjustment of curves
Calibration curve graph
Extra washing option to avoid interference and self-interference
Automatic sample re-dilution
Verification of reagent condition
Data import and export to interface with administration program
Reagent consumption statistics
Print outs of technical reports and patient reports
QC graphics
3.7 Power requirement

100- 240 VAC and 50- 60 Hz. 400 VA

3-3
3.8 Description of electronic circuits
The Analyzer is a completely micro-controlled instrument. The fundamental concept regarding its
operating way is that it carries out commands it receives through communication port. These
commands are simple operations sent by the user’s software on a PC, and the instrument decides
when they are to be carried out.
In order to carry out all the commands received from the PC, the Analyzer could have even twelve
microcontrollers. The main microcontroller receives commands through the communication port
and it sends the operations that correspond to the other eleven microcontrollers. Within the other
microcontrollers, there are six whose function is to operate the engines in the instrument, two which
control temperature and three which communicate with internal devices of the instrument.
The Analyzer has four power supplies: a 24 Volt power supply that provide power to the power
circuits; a ±15 Volt power supply for supplying power to the lamp and the analogical circuits, a 24
Volt power supply with output of 24 volt for the ISE module and 5 volt for digital circuit.
3.9 Types of Samples

The samples to be analyzed may be:
Plasma
Serum
Whole blood
Urine
Spinal Fluid
Puncture Fluid
Different biological fluids
Chemical solutions
3.10 Types of Reactions

End point reagent blank
End point sample blank
Kinetics
Fixed time kinetics
Ion Selective Electrode (ISE) (Optional)
3.11 Types of Methods

Colorimetric
Kinetics
Turbidimetric
Ion Selective Electrode (ISE) (Optional)

3-4
4 GENERAL FUNCTIONS
4.1 Main parts or modules
InCCA Bit Autoanalyzer can be divided into the following main parts:
Sample/ Reagent Tray

Dispensing Arm and Probe
Cuvette/Reaction Tray
Hydraulic System
Optical System
4.1.1 Sample/Reagent Tray
Figure 4-1
This tray can hold up to 24 reagents.
The Sample/Reagent tray can hold up to 22 samples in one loading. The software allows the
continuous loading of samples once the requested tests have been finished.
Primary tubes can be used since the capillary of the probe only reaches 1 mm below the sample
level. This increases laboratory biosafety. Cups for pediatric samples can also be used.

4-1
WARNING: DO NOT use container or tubes other than the ones suggested by
the manufacturer. (ie. Do not use eppendorf, do not use tubes inside the reagent
containers) Failing to accomplish this might affect directly the performance of the
analyzer and may cause failures of it.
4.1.2 Dispensing Arm and Probe
Figure 4-2
The dispensing arm transports the reagents and samples through the probe to a cuvette in the
reaction tray. Reagents and samples are preheated in this arm before being dispensed.
The probe has a conductivity detection system. When the probe is 1 mm below the surface of a
conductive liquid, it stops its movement. Due to this characteristic, it is important that samples do
not have bubbles since the level would be detected and only air would be aspirated, producing an
inaccurate result. It is also essential that samples are free of clots and fibrin.

4-2
4.1.3 Cuvette/Reaction Tray
Figure 4-3
This is where the reactions are carried out and read.
It has 100 separate cuvettes, grouped in 10 strips of 10 cuvettes. The cuvettes are made of PMMA,
which allows a good transmittance in the UV range.
The tray is heated by an air bath to 37ºC. All reactions are carried out at 37ºC.
Each cuvette has a minimum reading volume of 220 µl, and a maximum reaction volume (physical
capacity of the cuvette) of 600 µl.

4-3
4.1.4 Hydraulic System
Figure 4-4
Outside wiew of diluter Cuvette – Washig station –reagent bottle
Its main components are the diluter, solenoid valve, washing station and tubing that connects the
wash solution container to the probe.
The diluter aspirates the reagent and a sample volume required, and dispenses them into the
reaction cuvette.
The diluter also conveys the wash solution from the wash solution container to the end of the probe
capillary. This allows the washing and purging of the tubing, the diluter and the probe.
It is important to highlight those reagents never reach the diluter’s body.
Figure 4-5

4-4
4.1.5 Optical System
Light source: a 12-Volt 20-Watt krypton-halogen lamp is used which has a high emission in the UV
range (320 nm-380 nm)
Collimating lenses: 3 plane-convex lenses are used.
Wavelengths: 340-380-405-450-505-546-578-600-650 and 700 nm and an extra filter as optional.
Beam splitter: Divides the primary beam into two secondary beams: one goes towards the sample
channel and the other goes to the reference channel.
Sample channel: The sample channel beam passes through the cuvette to the sample photosensor,
where the signal is read.
Reference channel: The reference channel beam is read by the reference photosensor. The reference
channel compensates possible light source fluctuations.
4.1.5.1 Photometric Graph
Figure 4-6
1. LAMP
2. PLANE-CONVEX LENS
3. STOPPER
4. FILTER WHEEL
5. BEAM SPLITTER
6. SAMPLE CUVETTE
7. SAMPLE PHOTOSENSOR
8. REFERENCE PHOTOSENSOR

4-5
4.2 Description of the Operating Cycle
The operating cycle can be divided into three parts:
Reaction Preparation Cycle
Incubation and Reading Cycle
Reaction Dispensing Cycle
4.2.1 Reaction Preparation Cycle

The dispensing arm moves to the sample/reagent tray, and the probe places itself over the reagent
to be aspirated. The arm lowers until the sensor of the probe finds liquid level and it aspirates the
programmed reagent volume.
The arm moves to the wash station, and the external washing of the probe capillary is carried out.
The arm moves to the sample/reagent tray and it places itself over the sample to be aspirated. The
arm lowers until the sensor of the probe finds liquid level and it aspirates the programmed sample
volume.
The arm moves to the cuvette/reaction tray and places itself over the cuvette where the reaction is
going to be dispensed. It dispenses the sample and the reagent together.
The dispensing arm moves to the washing station, and the internal washing of the tubing and the
probe is carried out by the liquid diaphragm pump.
4.2.2 Incubation and Reading Cycle

In this cycle, incubation and photometric readings of the reaction are carried out at the appropriate
wavelengths.
The incubation and reading cycle occurs while a new reaction preparation cycle is beginning.

4-6
5 BASIC OPERATIONS
5.1 Using Commands Console
This console allows the interaction between the PC and analyzer.

To access, go to Maintenance -> Communications -> Commands console
There are two ways of executing commands:
 Through the Commands list
 Through the Terminal line
Figure 5-1
Commands list:
This consists of a list with almost all the available commands. Once the command is selected on the
command field, it’s necessary to click on EXECUTE (placed on the right of parameters field) to send the
command to the analyzer.
There’s an extra field to fix the parameters in case the command admits them.
Terminal line:
Here it’s necessary to enter the full command with the keyboard.
To do this, use the syntax detailed on chapter 11. Then click on EXECUTE (placed on the right of terminal
field).
Note that, in this field it’s possible to paste an extract of text to perform a commands sequence.
CAUTION: When executing a command from the commands list, the analyzer must be initialized,
otherwise the equipment will attempt to initialize. On the contrary, the terminal line allows executing a
comman<d without the restriction of running the initialization.
5-1
Next is described the communication protocol for Technical Service use.
5.2 Communication Protocol for Technical Service use
5.2.1 Introduction
The autoanalyzer will be communicated to the PC through a serial port RS232C by means of bidirectional
messages whose structure is described as follows:
5.2.2 Message Structure

Commands between the PC and the autoanalyzer will have the following structure:
{Command} {SP} {For} [{SP} {Argument}] {\r}
Where the field {Command} is described bellow. {SP} is the space character (ASCII 32), {For} is the
destination name for the message (for ex: A1, A2, etc.), {Argument} is an optional field that depends upon
the command and {\r} is the return character (ASCII 13) and corresponds to the end of the message.
Execution of a command starts when the PC sends a message to the Autoanalyzer corresponding to a
command (the command list is described in Command Description). The autoanalyzer answers by sending
an answer message where it reports the processing given to the received command, as described in
Answer Description. If the command requires transmitting complementary information to the PC, it will
afterwards send an asynchronous response, as described in Asynchronous answer Description.
5.2.3 Commands Description

The following is a description of the commands, grouped by module of the autoanalyzer.
Sample Probe
Description Command Alias Argument
Initialize vertical movement ProbeInit pbI No
Searching upper sensor ProbeOut pbOut No
Searching level sensor ProbeIn pbIn No
Decrease steps probeMoveIn pbMI uint steps
(optional)
Decrease steps not detecting level probeMoveInWithoutLevel pbMIWL uint steps
(optional)
Increase steps probeMoveOut pbMO uint steps
Starting horizontal movement probeArmInit pbAI No
Move steps to the left probeArmMoveClock pbAMC uint steps
Move steps to the right probeArmMoveCclock pbAMCc uint steps
Position on cuvettes for dispensing probeGoDispense pbGD No
Position on washing funnel probeGoFunnel pbGF No
Position on sample probeGoSample pbGS No
Position on reagent A probeGoReagentA pbGRA No

5-2
Reaction Tray
Initialize reaction tray reactionInit rI No
Cuvette to dispensing position reactionGoDispense rGD uint cuvette
Cuvette to photometer reactionGoPhoto rGP uint cuvette
Cuvettes forwards reactionForward rF uint cuvette
Cuvettes backwards reactionBackward rB uint cuvette
Advance steps reactionMoveForward rMF uint steps
Steps backwards reactionMoveBackward rMB uint steps
Pump
Initialize pump PumpInit pmI int steps
Advance steps pumpMoveForward pmMF int steps
Backward Steps pumpMoveBackward pmMB int steps
Pump pulse pumpPulse pmP int pulses
Heater
Set temperature HeaterSet hS float Temp
Switch on heater heaterPowerOn hPOn No
Switch off heater heaterPowerOff hPOff No
Read temperature heaterRead hR No
Preheater
Set temperatura preheaterSet prS float Temp
Switch on preheater preheaterPowerOn prPOn No
Switch off preheater preheaterPowerOff prPOff No
Read temperature preheaterRead prR No
Photometer
Initialization PhotoInit phI No
Go to filter photoSetFilter phSF int lambda
Read photoRead phR No
Switch on lamp LampHigh lH No
Switch off lamp LampLow lL No

5-3
Diluter
Initialize siringe SyringeInit sI No
Collect (relative)volume syringeAspirate sA float volume uint
veloc
Dispense(relative)volume syringeDispense sD float volume uint
veloc
Fix (absolute) volume SyringeSet sS float volume uint
veloc
Initialize valve ValveInit vI No
Move valve to pump or syringe ValveSet vS Probe / Pump /
ByPass
Miscellaneous
Select queue SetQueue -- uint queue
Delete all queues FlushAll -- No
Delete selected queue Flush -- No
Calibrate photometer calibratePhoto cP No
Calibrate cuvette calibrateCuvette cC No
Read container level vesselStatus -- No
Read Firmware versions firmwareVersionsRead -- No
Delete EEPROM eepromClear -- No
Delete cuvette absorbances from eepromClearAbs -- No
EEPROM
Write EEPROM eepromWrite -- uint Address
{byte/int/
uint/long/
float/string} Data
Read EEPROM eepromRead -- uint Address
Write a byte for EEPROM eepromWriteByte -- uint Address
Read a byte from EEPROM eepromReadByte -- uint Address
Write EEPROM version eepromVersionWrite -- uint Address
Read EEPROM version eepromVersionRead -- uint Address
Wait Delay -- uint mseg
Send a non executable message debugMessage -- Unspecified
amplifier disable disableVars -- A
Disabling Movement Reaction Tray R
Run sequence sequenceRun -- No
Sequence start marker sequenceStart -- No
Reaction Mixing cuvetteContentMixing cCM

5-4
Reset maintenance counter resetLevel -- uint Level
Enable reset counters resetLevelEnable -- 1/0
System Status Report systemStatus -- No
Analyzer Power On powerOn -- No
Analyzer Power Off powerOff -- No
The kinds of variable are:

byte: integer, 8 bits, no sign.
int: integer, 16 bits with a sign.
uint: integer, 16 bits no sign.
long: integer, 32 bits a sign.
float: floating point.
string: character chain.
A command for the probe until detecting level would be:

probeIn PC A1\r
5.2.4 Synchronous Answer Description

Once it has received the command, the Autoanalyzer sends an answer message to the PC. The word
indicating that it is an answer is status and the argument will reflect the result of the processing
performed by the autoanalyzer’s main controller with the command. Possible arguments are:
Argument Description
OK The command was correctly received and will be executed
SYNTAX the command received has a syntax mistake and must be resent by the PC
BUSY The Autoanalyzer is busy and cannot execute the command received,
which must be resent by the PC
QUEUED The command received cannot be immediately executed by the
Autoanalyzer, but has been placed in the queue
UNKNOWN the command received is unknown by the Autoanalyzer
The Autoanalyzer’s answer to the command in the previous section would be:
status A1 PC OK\r
5.2.5 Asynchronous Answer Description
If the commands involve a change in the Autoanalyzer’s state, (for ex. Tray or arm movements,
temperature reading, etc.) the Autoanalyzer will notify the PC in an asynchronous way once the command
has finished. This notice will exist, no matter whether the change of state is actually produced (for ex.:
move the tray). If the change of state affects several variables, the Autoanalyzer will send the PC an

5-5
asynchronous answer for each affected variable (for ex.: the command reactionMoveBackward will
generate the responses reactionCurPos, reactionCurDispense and reactionCurPhoto). In these cases, the
execution time will only be sent in the first answer. The following are the responses corresponding to the
commands involving changes in the Autoanalyzer’s state:
Command Answer Argument

probeInit ProbeCurPos int steps
probeOut
probeMoveIn
probeMoveInWithoutLevel
probeMoveOut
probeIn ProbeCurPos int steps
reagentAsingleCurVol (2) float mL
reagentAdoubleCurVol (2) float mL
reagentBCurVol (3) float Ml
noSampleDetected (4) int position
probeArmInit probeArmCurPos int steps
probeArmMoveClock ProbeArmCurVal Cuvette /
probeArmMoveCclock Funnel /
probeGoDispense Sample / ReagentA /
probeGoFunnel ReagentB / None
probeGoSample
probeGoReagentA
probeGoReagentB
reactionInit ReactionCurPos int steps
reactionGoDispense reactionCurDispense int cuvette / 0
reactionGoPhoto ReactionCurPhoto int cuvette / 0
reactionForward
reactionBackward
reactionMoveForward
reactionMoveBackward
srReadCode SrCurCode string Bar Code
pumpMoveForward PumpRelMove int steps
pumpMoveBackward
pumpPulse
heaterRead HeaterCurVal float Temp
heaterSet HeaterCurSet float Temp
heaterPowerOn Heater On / Off
heaterPowerOff
preheaterRead preheaterCurVal float Temp
preheaterSet preheaterCurSet float Temp
preheaterPowerOn Preheater On / Off
preheaterPowerOff
photoInit PhotoCurVal Black / int lambda /
photoSetFilter None

5-6
photoRead photoCurRead long int sample
long int reference
lampHigh Lamp High / Low
lampLow
syringeInit syringeCurPos float volume
valveInit ValveCurVal Probe / Pump /
ByPass
syringeAspirate syringeCurPos float volume
syringeDispense
syringeSet
valveSet ValveCurVal Probe / Pump /
ByPass
setQueue Queue uint queue
calibratePhoto photoCalibration OK
calibrateCuvette cuvetteCalibration OK
cuvetteContentMixing cuvetteContentMixed OK
vesselStatus waterVessel XX%
residuesVessel
concentratedResiduesVessel
systemStatus analyzerCycles long Cycles
analyzerHours long Hours
serviceLevel1 long Cycles
lampHours long Hours
eepromClear EepromClear OK
eepromClearAbs eepromClearAbs OK
eepromWrite EepromWrite OK
eepromWriteByte
eepromRead EepromRead {byte/int/uint/
eepromReadByte long/float} Data
eepromVersionWrite eepromVersionWrite OK
eepromVersionRead eepromVersion string version
resetLevel ResetLevel OK
resetLevelEnable ResetLevel Enable / Disable
eepromReset EepromReset OK

5-7
firmwareVersionsRead MainVersion string version
eepromVersion string version
serialNumber string version
verticalVersion string version
horizontalVersion string version
reactionVersion string version
sampleReagentVersion string version
filterWheelVersion string version
washerVersion string version
pumpVersion string version
amplifierVersion string version
heaterVersion string version
preheaterVersion string version
diluterVersion string version
powerOn power On
powerOff Power Off
coolerOn cooler On
coolerOff cooler Off
(2)
Answer sent when the arm is positioned over reagent mouth A.
(3)
Answer sent when the arm is positioned over reagent mouth B.
(4)
Answer sent if no liquid level detection when the arm is positioned on the sample tube or dispensed.
After the arguments described, there can be a comment field that will be identified by the character ‘*’
(asterix: ASCII 42).
Finally, the autoanalyzer will inform the PC about the time when the required command was completed
(measured from the moment the equipment was switched on). This field will be identified by the character
‘@’ (ASCII 64) and it will have the format h:m:s where h is hour, m is the minutes and s is the seconds
passed (expressed with three decimals).
If it were necessary, the Autoanalyzer could send “debug” messages by means of the debugMessage
response, which will be added after the asynchronous answer requiring additional information.
There may happen that the PC starts sending a command while the Autoanalizador is delivering an
asynchronous response. In that case, the Autoanalyzer will finish sending the asynchronous answer and it
will deliver the status message afterwards.
5.3 Command Console - Unit Quick Check -

The different modules can be quickly verified and Serial Port log can be visualized in the Command Console
at the same time. By means of the Command Console we can execute commands to initialize, enter
parameters to execute movements, activate or deactivate the lamp, check heater or preheater

5-8
temperature, or execute other commands. Just select the command, give the parameter (if necessary)
and, finally, execute.
To enter this test, go to the Maintenance menu, Communications and the Command Console. The
following image will be displayed on the screen:
Figure 5-2
5.4 EEPROM (Electrically Erasable Programmable Read-Only Memory)

version 1.09
For safety reasons the writing of eeprom a previous step is due to make. In the commands console,
in the Terminal line you have to write:
eepromWriteEnable A1 1

5-9
Main Controller
Variable Address Type
Serial number 1 String
EEprom version 8176 String
Sample Probe Vertical Movement

Cuvette Base 16 Int
Funnel Base 18 Int
Sample tube Base 20 Int
Reagent A container Base 22 Int
Reagent B container Base 24 Int
Starting Frequency 26 Int
Maximum Frequency 28 Int
Step Repetition 30 Byte
Cuvette Base/ Mixing 32 Int
ISE Base 34 Int
ISE Base for Aspiration 36 Int
Sample Probe Horizontal Movement

Dispensing 48 Int
Funnel 50 Int
Samples 52 Int
Reagent A 54 Int
Reagent B 56 Int
Starting frequency 58 Int
Maximum frequency 60 Int
Step repetition 62 Byte
ISE 64 Int
Reaction Tray
Steps per cuvette 80 Int
Cuvette 1 dispensing Offset 82 Int
Cuvette 1 photometer Offset 84 Int
Automatic movement 91 Byte

5-10
Sample/Reagent Tray
Number of sample tubes 112 int
Steps per sample tube 114 int
Number of reagent containers 118 int
Steps per reagent container 120 int
Steps between reagent racks 124 int
Filter Wheel
Steps per filter 144 Float
Offset filter 1 148 Int
Reading Delay 150 Int
Optical path 152 Float
Sample channel reading threshold 161 Long
Reference channel reading threshold 164 Long
Absorbance cuvettes threshold, warning. 167 Long
Absorbance cuvettes threshold, error. 171 Long
Gain threshold 175 Byte
Diluter
Total volume steps 176 Int
Syringe Volume 178 Int
Ramp 184 Byte
Pump
Pump steps per pulse 213 Int
Heater
Proportional gain 240 Int
Integral gain 242 Int
Derivative gain 244 Int
Oversampling 246 Byte
Automatic switching 247 Byte

5-11
Proportional limit 248 Int
Integral limit 250 Int
Derivative limit 252 Int
Temperature 254 Float
Temperature Offset 256 Float
Preheater
Proportional gain 272 Int
Integral gain 274 Int
Derivative gain 276 Int
Oversampling 278 Byte
Automatic switching 279 Byte
Proportional limit 280 Int
Integral limit 282 Int
Derivative limit 284 Int
Temperature 286 Float
Temperature Offset 288 Float
Containers
Zero waste container (1) 341 Int
Maximum waste container (1) 343 Int
Waste container volume (1) 345 Byte
Zero probe washing container (2) 346 Int
Maximum probe washing container (2) 348 Int
Probe washing container volume (2) 350 Byte
(1)
Reported as residuesVessel in the related asynchronous answer
(2)
Reported as concentratedResiduesVessel in the related asynchronous answer
Sample Barcode Reader (OPTIONAL)

Reader Enabled 368 Byte
Code Length 369 Int
Filters
Filter 1 2 3 4 5 6 7 8 9 10 11 12
Wavelength 512 544 576 608 640 672 704 736 768 800 832 864 Int

5-12
Sample gain 514 546 578 610 642 674 706 738 770 802 834 866 Byte
Ref. gain 515 547 579 611 643 675 707 739 771 803 835 867 Byte
Log I0 516 548 580 612 644 676 708 740 772 804 836 868 Float
Sample Blank 520 552 584 616 648 680 712 744 776 808 840 872 Long
Ref. Blank 523 555 587 619 651 683 715 747 779 811 843 875 Long
Zero Sample 526 558 590 622 654 686 718 750 782 814 846 878 Long
Zero Ref. 529 561 593 625 657 689 721 753 785 817 849 881 Long
Cuvette absorbances
Filter 1 2 3 4 5 6 7 8 9 10 11 12
Cuv. 1 2048 2052 2056 2060 2064 2068 2072 2076 2080 2084 2088 2092 float
Cuv. 2 2100 2104 2108 2112 2116 2120 2124 2128 2132 2136 2140 2144 float
Cuv. 100 7196 7200 7204 7208 7212 7216 7220 7224 7228 7232 7236 7240 float
The formula to calculate the corresponding EEPROM Address for a cuvettes absorbance at a certain
wavelength is:
(CUVETTE NUMBER - 1) * 4 * 13 + (FILTER NUMBER - 1) * 4 + 2048
ISE Module (OPTIONAL)

Variable Type
Address
ISE Enable 1024 int
ISE LI Disabled 1025 int
ISE NA Disabled 1026 int
ISE K Disabled 1027 int
ISE CL Disabled 1028 int
ISE CAL Time 1029 int
ISE Clean Time 1033 int
ISE CAL Temp Degrees 1037 float
ISE Offset Temp Degrees 1039 float
Lithium Electrode Serum Correlation – A parameter 1056 float
Lithium Electrode Serum Correlation – B parameter 1060 float
Sodium Electrode Serum Correlation – A parameter 1064 float
Sodium Electrode Serum Correlation – B parameter 1068 float
Potassium Electrode Serum Correlation – A parameter 1072 float
Potassium Electrode Serum Correlation – B parameter 1076 float
Chlorine Electrode Serum Correlation – A parameter 1080 float
Chlorine Electrode Serum Correlation – B parameter 1084 float

5-13
Sodium Electrode Urine Correlation – A parameter 1088 float
Sodium Electrode Urine Correlation – B parameter 1092 float
Potassium Electrode Urine Correlation – A parameter 1096 float
Potassium Electrode Urine Correlation – B parameter 1100 float
Chlorine Electrode Urine Correlation – A parameter 1104 float
Chlorine Electrode Urine Correlation – B parameter 1108 float
Miscellaneous
Hardware message enabling 400 int
Threshold reagent detection 403 int
Quantities stored of working hours. 404 long
Quantities stored of lamp working hours. 408 int
Quantities stored of cycles or testing. 410 long
Hours assigned (xh) 414 int
Cycles assigned (xc) 416 long
Multiplier for level 2 (N) 420 Byte
Multiplier for level 3 (M) 421 Byte
Service level1 422 long
Service level 2 426 long
Service level 3 430 long
Service express by hours 434 long
Frequency of message 439 Byte
Offset Level1 440 long
Lamp Stand By 452 Byte
Configuration 500 Byte

5-14
5.5 Obtaining Firmware Parameters
In order to obtain module parameters of factory adjustments, you have to enter the Maintenance screen,
select “Settings” and, finally, “Memory”.
All fields will be displayed in blank. Press the button Get from instrument to obtain parameters values. We
will be able to check the percentage of information transference on the progress bar. Once the
transference has finished, all fields will have been filled with the factory adjustment values.
Figure 5-3
To visualize the other parameters, you will only need to select on the module to be checked.
Note: it is highly advisable to keep a copy of the original equipment parameters together with the
autoanalyzer’s installation report.

5-15
6 DIRECTIONS FOR ASSEMBLING AND DISASSEMBLING
Assembling and disassembling modules
Autoanalyzer is made of modules; each module can be easily disassembled to perform total or partial
replace.
WARNING: before disassembling, turn the equipment off and disconnect the interlock from the
supply line. Remove reagents and put them into the refrigerator. Remove samples and proceed to
decontaminate the equipment and waste container according to good laboratory practices.
6.1 Disassembling the cabinet
a. Remove the cover from Cuvettes tray and SR/sample sector.
WARNING: before continued please check if the probe is at top of vertical position. If probe is
bellow the top, please take out the cover of arm removing the four screws M3 in both sides of this, and
pull up header module manually.
b. Turn the robot´s arm in an anticlockwise direction. Until on dispense position on cuvette tray.
c. Remove the top cover by removing the allen screws M4, there are five of them: two in the front
under the grid support adherent on top cover, two in the back side and one in middle under right
side. Note: unplug the keypad before remove completely the top cover
d. Remove the front cover, remove both laterals screws that hold the front cover(Allen M3), then,
move the analyzer a little bit forward in order to reach the screws located in the bottom of the
analyzer (three allen screws M3).
Important: Manually position the header on the funnel before turning on the equipment.
InCCA BIt Service Manual - MSv160101
6-1
6.2 Robot/Header Module
a. Remove 2 allen screws in the top cover of the robot and 2 allen small screws in both sides of cylindric
hood.
b. To remove the header module, remove the 2 screws allen M5 on aluminium block. Disconnect the main
power preheater , pcb board preheater cable on J3 connector, and pre heater teflon tubing.
c. Remove the two parts of cover, sliding over these in opposite direction and pull up the cylindrical hood.
Note: If the header module has not removed, you need pass whole the cylindrical hood through the
header module and probe.
d. Disconnect the 3 connectors in the end of plastic spring and Teflon tubing in solenoid valve outlet. Do
not disconnect the vertical and horizontal movement detector connectors. Loosen the clamp of plastic
spring.
e.Remove the Robot/Header Module; remove the four allen screws M6 on the back of the analyzer, in the
same place than the general base.
f. No adjustment is required to assemble the module. Fix it to the bottom base by the four screws, fix the
fixing screws firmly. Continue with the assembly.
g. Perform a precision test (section 11, Validation Program) in order to verify correct assembling of the
arm. Purge of the hydraulic system should previously be made.
Note: Without totally removing the Robot / Header module from the autoanalyzer’s base the header can
be disassembled (point “b”). Once it has been assembled, it is important to verify the right position of the
Sample/ Reagent probe in the dispensing position.
6.3 Sample/Reagent Module
a. Remove the Samples tube sector pulling up, it is fastened at the base with a magnetic guides.
b. Remove the Reagents sectors pulling up, it is fastened at the base with a magnetic guides.
6.4 Cuvette Module

This module has the Photometer inclued.
a. To remove the whole Cuvette module, the plastics clamps holding the wires must first be removed
(do not cut with pliers). There are two behind the pcb boards on the electronic board rack.
b. Disconnect connectors J9, J10, J11, J12, J15 (Pos.1 and 2 set of brown and white wires), J16, J17
from the PCB Controller and connectors J2 and J8 from the PCB Regulator, corresponding to the
set of wires on the photometer’s side. Note: for safety, some cables are fixed with seals at
electronic board rack, cut these to remove the cables and after replace these when installed again.

6-2
c. Disconnect the discharger tube on bottom base, in left front end side.
d. Remove the 4 M6 fixing nuts in the Cuvette module.
e. Remove the module.
f. No adjustment is required for the Cuvettes module assembly
g. Proceed to assembly
NOTE: To change Home position sensor, it is necessary to remove the cuvette supporting disk. This disk
has only one position, which makes its assembly easier. To remove the Washer and Photometer modules,
it is not necessary to completely remove the Cuvette module.
6.5 Photometer Module

a. Remove the 2 screw allen M4 on the lamp disipator cover in top side of photometer and pull up
this.
b. Remove the 4 screws allen M4 , 2 in right side and 2 in left side of photometer cover and pull up
this.
c. Remove 2 screws allen M3 in chamber reaction union and remove this part.
d. Remove the 2 screws llem M4 on the bottom of module in both sides of optical cover between
photometer and reaction chamber.
e. Remove the 2 Allen M6 screws assembled on the bottom at the level of the base of the Cuvette
module. Remove the Photometer module.
f. Disconnect the flat cable in sample amplifier connector and remove the pcb board sample
amplifier unscrewing the 2 screws allem M3 on pcb board surface.
g. Remove the optical of sample amplifier unscrewing the hexagonals abutments.
h. Disconnect the cable in filterwheel sensor (J1 connector), lamp coennector, flat cable of reference
amplifier, cable of sensor LM35 and cable on connector J1 on heater pcb Borad.
i. In order to assemble the Photometer module, alignment of the optical path in relation with
reaction cuvette should be performed. Align optical path with reaction cuvette to assemble
photometer module
j. Pull up the photometer module.

6-3
k. Once assembly and alignment of the optical path has finished, run the Validation Program.
l. Perform Photometer and Cuvette Calibration (for all cuvettes) from the user’s program.
Maintenance, Equipment
6.6 Electronic Module
It is located on the front part of the autoanalyzer. The printed circuit plates are assembled on a metal
support (electronic board rack). Interconnections between PCB and the electrical supply are made through
connectors.
The plates forming the electronic module are: PCB CONTROLLER, PCB REGULATOR and DC INTERRUPTOR
(revers side of rack). There are also 24 Volts, 15 Volts and +/-15 Volts commutated electrical supplies in
the back side of autoanalyzer.
Figure 6-1
PCB Controller PCB Regulator

6-4
PCB Controller
Note: The Controller plate is assembled on the metal support by 5 Allen screws. The Regulator plate and
DCInterruptor plate is assembled by 4 Allen screws, two on each side.
PCB Regulator
Figure 6-2

6-5
PCB DC Interruptor
Figure 6-3
6.6.1 Diluter
a. Disconnect J13 and J14 connectors on the PCB Controller board, after unplugging, cut seals to
remove the cables at electronic rack.
b. Disconnect Teflon tubing on top of module.
c. Unscrew the 2 Allen (M3) screws behind the diluter with allen wrench across the holes on each
sides of diluter support, push down slowly until to end of slide.
d. Pull out the diluter to remove it.
e. No adjustment is required for this module assembly.
f. Perform several purge cycles at restart system.
6.6.2 Solenoid Valve N.O.

a. Disconnect J33 connector of the PCB Controller.
b. After unplugging, cut whit careful the seals to remove cable to electronic rack.
c. Remove the tubings from each side of valve.
d. Remove the 2 Allen (M3) screws from front side of plastic cube.
e. No adjustment is required for this module assembly.
f. Perform several purge cycles

6-6
7 MODULES ADJUSTEMENT
The firmware calibration parameters sequence corresponding to the different modules is described in this
section. It is a referential sequence which can only be performed on the necessary points.
In order to perform the adjustments it is important to consider the values obtained from Firmware (see
section 5.4 of the Technical Service Manual, “Obtaining Firmware Parameters”). These values are the
default made of manufacturer (if it is the first time the instrument is calibrated).
Adjustment values for each module can also be consulted from Commands Console, by executing the
command eepromRead. We can enter new adjustment values by means of the command eepromWrite,
(see section 5.3 of the Technical Service Manual to find the corresponding EEPROM address for each
variable.
Note: Initialize all modules before checking or adjusting each module. It’s a good practice use the
command eepromRead for take note about the value of address relative at adjustement before modify
this one.
7.1 Reaction Tray Dispensing Offset Calibration
This calibration adjusts the Reaction Tray’s position at the moment of Dispensing by the Robot Arm into
the reaction cuvette. The reference point for this adjustment is the relative position between the cuvette
number one and probe on dispense position, and this adjustment is independent from the photometer’s
calibration (Reaction tray photometer offset).
1. Remove reaction tray cover.

2. Open Command Console
3. Execute command reactionInit.
4. Execute the command probeGoDispense.
5. Execute command probeMoveIn.
6. Verify if the tip of probe is in centre of cuvette number one.
7. Otherwise, correct the position by executing the command reactionMoveForward or
reactionMoveBackward and enter the number of steps you want to move (in parameters) it in a
relative way, clockwise or counter clock wise.
8. Once the wanted position is obtained, you could check the new parameter in the Asynchronous
Answer, “reaction CurPos xxx”.
9. Execute the command eepromWrite A1 82 XXX (Remember send eepromWriteEnable A1 1,
Chapter 5.4) where XXX is the new position obtained (point 8).
10. Restart the reaction tray and repeat the operation if necessary.
Important: (About step number 10) this will allow us to check the correct position of the adjustment
performed, as well as the stability of the Reaction Tray’s movement.
Note: First cuvette is indicated by an arrow. A complete turn of the Reaction Tray is equal to 2400 steps.
As the Reaction tray has a capacity for 10 strips, then the number of steps per cuvette is 24.
7-1
7.2 Reaction Tray Photometer Offset Calibration
This calibration is the adjustment of the Reaction Tray in relation to the light beam on the Photometer‘s
Sample Channel. This adjustment is independent from dispensing adjustment (Dispensing offset).
Initialize the Reaction Tray. Before performing this adjustment, check the position of the Reaction Tray
against the cuvette one (reference position).
1.Remove reaction tray cover.

2.Open Command Console
3.Execute command reactionGoPhoto, to position cuvette Nº1 in front off photometer.
4.Execute command photoSetFilter and choose a lightwave easy to view across cuvette, for example
546 nm, writing this one in parameters as a number, for example: 546. The light beam must be
centred on the cuvette.
5.Otherwise, correct the position by executing the command reactionMoveForward or
reactionMoveBackward and enter the number of steps you want to move (in parameters) it in a
relative way, clockwise or counter clock wise.
6.Once the wanted position is obtained, you could check the new parameter in the Asynchronous
Answer, “reaction CurPos xxx”.
7.Execute the command eepromWrite A1 84 XXX (Remember send eepromWriteEnable A1 1, Chapter
5.4) where XXX is the new position obtained (point 8).
8.Restart the reaction tray and repeat the operation if necessary. Check that asynchronous answer
referred to dispensing position is 0 (reactionCurDispense A1 PC 0).
There is a sequence that can be executed through the Commands Console, writing that in TERMINAL slot,
after dispensing washing solution in cuvette number one, performs several readings on the cuvette using
three different filters, and then calculates with each filter the position of the cuvettes tray in front of the
photometer that better fits, and that is the one that has to be written in address 84. This sequence is
called “zSeq CuvettePeaks” and consists on the following commands:
reactionGoPhoto A1 1
photoSetFilter A1 340
zSeq A1 cuvettePeaks
Once executed you will get an answer similar to the following for each filter:
rPhotomOffset = 1871 -> 1870
This means that the current offset is 1871 and should be changed to 1870, meaning that you should write
1870 in address 84. Usually the same value will be obtained for the three different filters.

7-2
Important: once the adjustment has been done, it is advisable to check positions twice or three times. This
will allow us to check the correct position of the adjustment performed as well as the stability of the
Reaction Tray’s movement.
7.3 Adjust robot arm reference position

To start the calibration of robot arm position, it requires that the probe is in centre of hole in black cover,
of robot estructure. Once to that condition is correct, then you have to follow all the sequences of
calibration for any probe position.
Fisrt, Initialize the movement of probe, you can be execute the commands probeInit and probeArmInit.
Second, check if the tip of the probe is located all inside the hole of black cover. You have to use the
interior side to black cover to fix the height of TIP of probe in reference to main robot axis. Just like is
showed in first picture of Figure 7-1
Finally, check if the screw M5 in the main metal cube it is tight respec axis, otherwise screwing this and
check handly if the robot header hold fixed. Also look the probe, must be in centre of hole in black cover
Figure 7-1

7-3
Warning: Then that, you must check all the horizontal´s and vertical´s positions always (Dispense,
Sample and reagents
7.4 Head Robot Position over Dispense Calibration
Execute the command to initialize the cuvette Tray and the commands to initialize vertical and horizontal
movement of the Robot Arm.
Then execute the command probeGoDispense; the Robot arm will directly move towards the Cuvette
Tray, positioning itself over the mouth on cuvette Nº1.The probe must be perfectly aligned over the
mouth. Otherwise, positioning must be corrected on the horizontal movement of the robot arm.
To adjust the horizontal position of the Robot arm, the commands probeArmMoveClock and
probeArmMoveCClock must be executed, moving the necessary steps. Once the desired position has been
obtained, execute the command eepromWrite A1 48 XXX where XXX is the newly obtained position.
To visualize better, execute the command probeMoveIn and enter 800 steps as argument. The probe must
be centered in relation to the cuvettes mouth.
Note: Once the adjustment has been performed, it is advisable to check positions twice or three times.
This will allow us to check the correct position of the adjustment performed as well as the stability of the
Robot Arm and the Cuvette Tray’s movement.
7.5 Cuvettes Base Vertical Movement Calibration
This calibration is meant to adjust vertical movement of the Robot Arm on the reaction cuvettes position
at Dispensing step.
Initialize Reaction Tray and Robot Arm, both vertical and horizontal and execute the command
probeGoDispense.
Execute the command probeMoveIn. The Sample / Reagent probe must be positioned about 1 mm. over
the reaction cuvettes mouth.
To correct position, the number of steps needed to adjust the Robot Arm in relation to the reaction
cuvettes mouth should previously be established. This value can be accurately established by means of the
commands probeMoveIn and probeMoveOut. Once the desired position has been obtained, execute the
command eepromWrite A1 16 XXX, where XXX is the newly obtained position.
Note: Once adjustment has been performed, it is advisable to check positions twice or three times. This
will allow us to check the correct position of the adjustment performed as well as the stability of the Robot
Arm’s movement.

7-4
7.6 Cuvettes Base Vertical Movement during Mixing Calibration
This calibration is meant to adjust vertical movement of the Robot Arm on the reaction cuvettes position
during Mixing step.
Initialize Reaction Tray and Robot Arm, both vertical and horizontal and execute the command
probeGoDispense.
Execute the command probeIn. The white cone of the Sample / Reagent probe must be positioned about 1
mm over the reaction cuvettes mouth.
To correct position, the number of steps needed to adjust the Robot Arm in relation to the reaction
cuvettes mouth should previously be established. This value can be accurately established by means of the
commands probeMoveIn and probeMoveOut. Once the desired position has been obtained, execute the
command eepromWrite A1 32 XXX, where XXX is the newly obtained position.
Arm’s movement.
Warning: This position has to be calibrated knowing that the probe will enter all the way into the
cuvette, so, if necessary, losen the four screws that hold the Cuvettes Tray Module and move it to the
necessary position.
7.7 Head Robot Position over Funnel Calibration
Note: The mechanical zero is previous at funnel, when move the arm from left to right.
Execute the command to initialize the cuvette Tray and the commands to initialize vertical and horizontal
movement of the Robot Arm.
Then execute the command probeGoFunnel; the Robot arm will directly move towards the funnel
positions.The probe must be perfectly aligned over the funnel. Otherwise, positioning must be corrected
on the horizontal movement of the robot arm.
To adjust the horizontal position of the Robot arm, the commands probeArmMoveClock and
To visualize better, execute the command probeMoveIn and enter 500 steps as argument. The probe must
be centered in the cup funnel.

7-5
Robot Arm and the Cuvette Tray’s movement.
7.8 Funnel Base Vertical Movement Calibration
To correct this level’s height, the number of steps needed to adjust the Robot Arm in relation to the funnel
should previously be established. This value can be accurately established by means of the commands
probeMoveInWithoutLevel and probeMoveOut. Once the desired position has been obtained, execute the
command eepromWrite A1 18 XXX where XXX is the newly obtained position.
Important: 10 steps, corresponds to 1 mm.
Note: once adjustment has been made, it is advisable to check positions twice or three times. This will
allow us to check the correct position of the adjustment performed as well as the stability of the Robot
Arm’s movement.
The purpose of this calibration is to adjust the robot Arm’s positioning over the funnel.
7.9 Header Robot Position over Reagent Calibration
The purpose of this calibration is to adjust Robot Arm’s positioning over mouth of Reagent Nº1.
Execute the command to initialize the Sample / Reagent Tray and the commands to initialize vertical and
horizontal movement of the Robot Arm.
Then execute the command probeGoReagentA; the Robot Arm will directly move towards the Sample /
Reagent Tray, positioning itself over mouth of the Reagent Nº1 container in position Nº1. The probe must
be perfectly aligned over that mouth. Otherwise, positioning must be corrected on the horizontal
movement of the robot arm.
To adjust horizontal position of the Robot Arm, the commands probeArmMoveClock and
NOTE : You have to adjust the “STEPS BETWEEN RACKS OF REAGENTS” , once the desired position has
been obtained for the reagent number 1, execute the command probeGoReagentA and put “16” in
parameter argument to check the horizontal position of probe over the mouth of reagent bottle number
#16 , then you have to use the same command with “17” in the parameters argument to check the
horizontal position of probe over reagent bottle number #17, execute the command eepromRead A1 124
to take note about the status of actual steps and if is necessary modify it using eepromWrite A1 XXX,
It is advisable to increase or decrease by one step the value obtained from the reading of the direction
124, until achieving the definitive adjustment.

7-6
For a better visualization, execute the command probeMoveIn and enter 300 steps as argument. The
probe must be inside the reagent container, centered in relation to the container’s mouth.
Arm movement.
7.10 Reagent A Container Base Vertical Movement Calibration
The purpose of this calibration is to adjust the vertical robot arm movement on mouth of the reagent
container.
Initialize the horizontal and vertical.
Execute the command probeGoReagentA. The Robot Arm will directly move towards the sample / reagent
tray, positioning itself on mouth A of reagent Nº1 container.
Then, execute the command probeIn, which will produce a movement of the probe according to a value
that has been set in EEPROM. The probe must be positioned the nearest possible to the bottom of the
reagent’s container without activating the impact detector, this allows working with the smallest dead
volume of reagent.
If modification of this value is intended, you can resort to the commands probeMoveIn and
probeMoveOut. Once the desired position has been obtained, execute the command eepromWrite A1 22
XXX where XXX is the newly obtained position. To check this position, you can put 1ml of liquid in the
reagent container and verify that the informed volume in the asynchronous answer is smaller than that
value.
Robot Arm’s movement.
7.11 Header Robot Position over Sample Tube Calibration
The purpose of this calibration is to adjust the positioning of the robot arm on sample tubes.
Initialize the vertical and horizontal movement of the robot arm.
Then, execute the command probeGoSample. The Robot Arm will move directly towards the sample /
reagent tray and will be positioned on the mouth of sample tube Nº1. The probe should be perfectly
aligned over the mouth. Otherwise, positioning would be corrected on the robot arm’s horizontal
movement.

7-7
To adjust horizontal position of the robot arm, the commands probeArmMoveClock and
probeArmMoveCClock should be executed, moving the necessary steps. Once the desired position has
been obtained, execute the command eepromWrite A1 52 XXX where XXX is the newly obtained position.
For a better visualization, execute the command probeMoveIn and enter 300 steps as argument. The
probe must be positioned inside the sample tube, centered in relation to the tube’s mouth.
Robot Arm movement.
7.12 Sample Tubes Base Vertical Movement Calibration
The purpose of this calibration is to adjust the vertical movement of the robot arm in sample tubes.
Initialize sample / reagent tray and robot arm, both vertical and horizontal.
Execute the commands probeGoSample and srGoSample. The Robot Arm will move directly towards the
sample / reagent tray and will be positioned on the mouth of sample tube Nº1.
Then, execute the command probeIn, which will produce a movement on the probe according to the value
that has been set at EEPROM. The probe must be the nearest possible to the bottom of the sample tube
without activating the impact detector, this allows working with the smallest dead volume of reagent.
If you want to modify this value, you can resort to the commands probeMoveIn and probeMoveOut. Once
the desired position has been obtained, execute the command eepromWrite A1 20 XXX where XXX is the
newly obtained position.
Robot Arm’s movement.
7.13 Waste Container Volume Calibration
Remove the waste container’s connector with its tubings. Execute the command vesselStatus and, from
the asynchronous answer residuesVessel, obtain the value after the letters CR (this value may be positive
or negative) Enter that value in EEPROM by executing eepromWrite A1 341 XXX where XXX is the value
that has been read its sign must be enter.
Put the connector and the tubings belonging to the waste container on a full container. Execute the
command vesselStatus and, from the asynchronous answer residuesVessel, obtain the value after the
letters CR (this value may be positive or negative) and subtract the value obtained before, respecting the
signs. Enter the result of the subtraction in EEPROM by executing eepromWrite A1 343 YYY where YYY is
that result respecting the sign.

7-8
7.14 Probe Washing Solution Container Volume Calibration
This meter is optional and may not be implemented in hardware. The related asynchronous answer is
referenced as “Concentrated Residues”.
Remove the Probe Washing solution container’s cap together with its tubings. Execute the command
vesselStatus and, from the asynchronous answer concentratedResiduesVessel, obtain the value after the
letters CC (this value may be positive or negative) Enter that value in EEPROM by executing eepromWrite
A1 346 XXX where XXX is the value that has been read respecting its sign.
Put the cap and the tubings belonging to the concentrated waste container on a full container. Execute the
command vesselStatus and, from the asynchronous answer concentratedResiduesVessel, obtain the value
after the letters CC (this value may again be positive or negative) and subtract the value obtained before,
respecting the signs. Enter the result of the subtraction in EEPROM by executing eepromWrite A1 348 YYY
where YYY is that result respecting the sign.

7-9
8 PARTS REPLACEMENTS
8.1 Light bulb replacement
1. Turn off the instrument.

2. Remove the top cover as explained in section 6.1
3. Remove the dissipater, removing the two screws.
4. Disconnect the lamp from the air connector by loosening the fastening screw. Remove the light
bulb.
5. Install the new light bulb, positioning the mark in black zocket of the lamp pointing up (the
filament holding in horizontal position in that way).
Caution: hold the light bulb by the base and don’t touch the glass of the bulb. Tighten the screw
slightly.
Caution: the filament should always be in a horizontal position. Once the proper adjustment has been
made, secure the position of the light bulb with the fastening screw.
6. Reconnect the lamp to the air connector.

7. Turn on the instrument.
8. Start up the photometer Maintenance  Communications  Console of commands  select
photoInit  Execute
9. Select a visible wavelength range, ex. 546 nm Maintenance  Communications  Console of
commands  select photoSetFilter  Parameters enter 546  Execute
10. Remove one strip of the cuvette in the optical path and place a white piece of paper against the
lens of the photometer. Adjust the position of the lamp such that the filament can be seen sharply
against the paper. In case of being necessary to fit the focal length moving the lamp, without
rotating it (to be able to maintain the filament in horizontal position), It must look for to focus the
beam in the center of the optical path of the cuvette. Once obtained the adjustment, fix the lamp.
8-1
11. Run a calibration of the photometer and all the cuvettes Maintenance  Instrument 
Calibration  select Calibrate photometer  select Calibrate cuvettes  First cuvette 1  Last
cuvette 100  select the position of the solution to use for the calibration ex: Use container 
Calibrate
12. Finally reset the lamp hour counter with commands:
resetLevelEnable A1 1
resetLevel A1 Lamp
Dissipater
screws
Dissipater
Figure 8-1
Silicon Grease
Light bulb
Fastening screw
Air Connector
Figure 8-2

8-2
8.2 Interferential filters replacement
1. With the equipment off, remove the top cover and right lateral of the autoanalyzer to have access
to the photometer, located on the left front side.
2. Remove the photometer’s upper lid.
3. Spot the interferential filter to be replaced and manually turn the filter’s wheel clockwisely to find
and to easily remove the interferential filter holder from the filter wheel.
4. Place the holder with the new filter in the filter wheel.
Filter holder
Figure 8-3
5. Replace the lids, switch on the equipment.

6. Calibrate the photometer from the Maintenance screen, “Photometer calibration” and select all
cuvettes.

8-3
8.3 Sample / Reagent probe replacement
1. Keep the equipment off, remove the orange hood of the Sample / Reagent arm.
Teflon tubing
Key Allen 2.5 mm
Fixing screws
Figure 8-4
2. Disconnect the connector from PCB and remove the fixing screws on the PCB. Disconnect the
Teflon tubing from the hydraulic connector To remove the Sample / Reagent probe and remove
the PCB

8-4
Figure 8-4
3. Replace the Sample / Reagent probe. The new probe has a polarizing pin to make assembly easier.
Pin
Figure 8-5
4. Assembly the PCB and connect the Teflon tubing.

5. Perform several purge cycles and check that there is no liquid leak.

8-5
Caution: Check when assembling the following items for proper replaces
Distance I same as distance II
Figure 8-6
Check angle of 90º
Figure 8-7

8-6
Right
Figure 8-8
Wrong
Figure 8-9

8-7
8.4 Cuvettes replacement

2. Remove all the cuvette strips, unscrewing the metal nuts.
Caution: avoid dropping the metal nuts into the interior of instrument during this procedure.
3. Put the new strips in place, tightening securely the metal nuts.
5. Run a diluter purge cycle Maintenance  Instrument  Diluter Purge
6. Calibrate all the cuvettes Maintenance  Instrument  Calibration  select Calibrate cuvettes
 Range 1 to 100  select the position of the solution to use ex: Use container  OK
8.5 Fuses Replacement
1. Turn off the instrument

2. Remove the feed cable from the line filter
3. Remove the lid of the fuse-box located in the upper part of the line filter
4. Remove the burnt fuses and replace them with new ones
5. Replace the lid of the fuse box
6. Reconnect the feed cable to the line filter

8-8
9 MAINTENANCE PROGRAM
In order to ensure optimal performance and maximum useful life of the Autoanalyzer, it is important
to follow the cleaning and maintenance instructions outlined in this section.
9.1 Daily Maintenance
9.1.1 Before starting daily operation
1. Verify that the levels of the waste and washing solution containers are adequate to start with
the daily operation.
2. Run two diluter purge cycles, visually checking the absence of bubbles in the diluter body
Maintenance  Instrument  Diluter Purge
3. Verify if all cuvettes are clean.
9.1.2 Upon finishing with the daily operation

1. Wash all the cuvettes
2. Run the cleaning of the TIP using a 30% commercial sodium hypochlorite solution in the
selected tube Maintenance  TIP Cleaning  OK
9.2 Weekly Maintenance
1. Wash all the cuvettes.

2. Run two diluter purge cycles Maintenance  Instrument  Diluter Purge
3. Calibrate all the cuvettes Maintenance  Instrument  Calibration  select Calibrate
cuvettes  Range 1 to 100  select the position of the solution to use ex: Use container 
OK
9.3 Monthly Maintenance
9.3.1 External washing of cuvettes

2. Remove all the cuvette strips, unscrewing the metal nuts.
Caution: avoid dropping the metal nuts into the interior of instrument during this procedure.
9-1
3. Wash the external part of the cuvettes under the faucet with detergent and plenty of water.
Rinse with plenty distilled water.
4. Dry the external part of the cuvettes gently with paper towel.
Caution: avoid scratching or leaving traces of paper inside the cuvettes during this procedure.
5. Place the cuvette strips on the tray, tightening securely the metal nuts.
7. Run two diluter purge cycles Maintenance  Instrument  Diluter Purge
8. Calibrate the photometer and all the cuvettes Maintenance  Instrument  Calibration 
select Calibrate photometer  select Calibrate cuvettes  Range 1 to 100  select the
position of the solution to use ex: Use container  OK
9.3.2 General washing of the instrument
1. Washing the containers: disconnect the waste and washing solution tubes and clean the
containers with plenty of water. Washing solution container: rinse with distilled water.
Waste container: rinse with commercial sodium hydrochloride 30% solution and plenty of
water.
2. Cleaning the reagent tray: turn off the instrument and the reagent refrigeration. Remove all
the reagent bottles and clean the reagent tray and sample tray with a damp cloth.
3. Cleaning the cuvette tray: clean the black surface of the cuvette tray with a damp cloth.
4. Cleaning externally: with a damp cloth, clean the external covers, lids, hood of the pipetting
arm.
Caution: the instrument needs to be turned off during this procedure. Take care not to spill
liquids on the instrument.
5. Cleaning the tip externally: clean the tip from top to bottom with paper towel dipped in
isopropyl alcohol.
Caution: When performing this procedure, avoid removing the PTFE cover of the volume
sensor probe.
9.3.3 Back-up of files in use

1. Select Methods  Save  an explore window will open  select the back-up file
destination for a removable drive, ex. USB (E:)  name the method file in use as methods
yymmdd.adb, where yy is the year, mm the month and dd the day for the date of the back-up
 Save
2. Repeat the same procedure for the calibrator and control files by going to the Calibrator and
Control menus.
3. Back-up methods, calibrators and controls files when these are configured for the first time
or whenever configuration changes are made.

9-2
4. Select Maintenance Menu  Settings  Enter password  Database tab  press Database
Copy  select the folder where the data will be saved  Copy
Figure 9-1
Warning: this procedure may take several minutes depending on the size of the
database, wait until the copy is properly finished otherwise the file created might be
corrupted or it might cause the unexpected shutting down of the software.
9.4 MAINTENANCE BASED ON ALARMS
Any change on the procedure could affect the correct working and, because of this would be very
important understand completely this document and follow, step by step, indications suggested.
9.4.1 ALARMS GENERATED BY COUNTERS
We have three independent counters, one of them count working hours and a second one, which do
the same with cycles or test number and a third, counting the lamp working hours.
As well as we know, a common wearing of machine depend on kind of user, that´s why, system is
able to differentiate on the correct level of maintenance to apply. System analyzes if maintenance is
done by the first counter or second one. Besides, the system puts on screen a different maintenance
levels depending on counters mentioned.

9-3
The system operation is explained next:
It works with two incremental variables. Cycles are embedded in the variable Xc and hours in variable
Xh.
Finally, two multipliers are added to increase to the level two and three to be differentiated from
level one.
Those multipliers are called M and N.
Having got last data we are able to write the system equation:
L1 = X
L2 = N . X
L3 = M . N . X
Example:
We want to have a preventive maintenance with 80000 test or 5000 hours for using. In other words,
the first event occurred between both of them. It´ll happen if variables are set as follow:
Xc = 80000 test or Xh =5000 hours
9.4.2 Enabling of Counters
System will be working correctly only if some variables are enabled. This is showed at ANEXO 11.6
Following data received from statistics we recommend to use:
XH=5000
XC=80000
N=0
M=0
NOTE: M and N multipliers are fixed on “cero” because of an autoanalizer InCCA Bit doesn´t need the
level 2 and Level 3 maintenances.
Let´s see how variables are loaded with the suggested values:
eepromWriteEnable A1 1
 Enable to write variables.

9-4
eepromWrite A1 414 5000
 Embed the quantities of working hours to be counted.
 Embed the quantities of cycles or test to be counted.
 Loading of values in “N” multiplier.
 Loading of values in “M” multiplier.


9.5 Level 1 Maintenance

Once counters carry specifications out, an alarm appears to show the necessary maintenance. Let´s
see an example:
9.5.1 General washing of the instrument
1. Cleaning the reagent tray: Turn off the instrument and the reagent refrigeration. Remove all
the reagent bottles and clean the reagent and sample tray with a damp cloth.
2. Cleaning the cuvette tray: Clean the cuvette tray with a damp cloth (the black surface).
3. Cleaning exterior of analyzer: with a damp cloth, clean the external cover, lids and orange
hood of the pipetting arm.
Caution The instrument needs to be turned off during this procedure. Take care
not to spill liquids on the instrument.
4. Cleaning the tip externally: Clean the tip from top to bottom with paper towel dipped in
isopropyl alcohol.
Caution: For this procedure, avoid removing the PTFE cover of the volume sensor
probe.

9-5
9.5.2 Lubricating the axels
2. Remove the top cover according to instructions given in section 6.1.
3. Lubricate with machine oil every bronze bushing of the equipment, axis robot, axis washer
and axis piston pump.
9.5.3 Initializing the counter
Once mentioned maintenance was done, We have to reset a counter internal mark to modify the
counter base.
To do this we use the following commands:
resetLevelEnable A1
resetLevel A1 1
Status verification
Now, we need to read the system status with the command.
systemStatus A1
This is the command which gives the actual information from instrument and, this is used to know
what kind of maintenance we need to do. For example, last command gives us the information about
lamp working hours. Therefore, if the counter time is near of the end time for lamp life we could
change the part (lamp) and evade another programmed visit for official service.
9.6 Maintenance According to Need
9.6.1 Database Initialization (every 10.000 determinations)

1. Select Maintenance Menu  Settings  Enter password  Database Refresh Statistics
In the lower part of the tab, are shown the numbers of patients, calibrators, and controls
samples for the database in use. The Total is also shown, with the inclusion of the previous
results. In the adjacent box the Total value corresponds to the sum of all the determinations in
the database in use. These numbers are updated with the Refresh Statistics command
Before the total number of determinations reaches the 10.000, the database must be initialized:
steps 2), 3) and 4)

9-6
Figure 9-2
2. Select Maintenance Menu  Settings  Enter password  Database Export results 

select the folder where the data will be saved. Name the file as results yymmdd.csv, where yy
is the year, mm is the month and dd is the day for the date of the back-up. Press Save and a
window will open for selecting the type of results to be saved: patient, calibrator and control
results. Select all the options and press Export. The next window allows the selection of the
entire database or just a range given by the date and the hour, or the process numbers
(initial and terminal) to use for saving the information. For the entire database select the
option All.
3. Select Maintenance Menu  Settings  Enter password  Database tab  press Database
Copy  select the folder where the data will be saved  Copy (this process may take
several minutes, the interruption of the process may cause the corrumption of the file
created)
4. Select Maintenance Menu  Settings  Enter password  Database  Initialize: this is

where the Patient results, Calibrators and Controls results are all erased
The last calibration is saved in the methods configuration, and the configuration dates of the
calibrators, controls and methods are not modified.

9-7
Figure 9-3
Press Yes in this tab, close and open software
9.6.2 Light Bulb Replacement

See Section 8.1
9.6.3 Cuvettes Replacement

See Section 8.4
9.6.4 Fuses Replacement

See Section 8.5

9-8
10 TROUBLESHOOTING
This Troubleshooting section provides help to solve different situations that may happen with the
autoanalyzer
For any problem other than those described here, contact exclusively the Technical Service
authorized by Audit Diagnostics.
Problems may be gathered in three main groups:
1. Inconsistent measurements
2. Visible failures
3. Malfunction problems, error flag will appear on the screen.
It’s also described in this chapter a diagnostic tool for technical service to use: the log.html files. This
files record the communication between the PC and the analyzer in given situations, for example,
when an error appear, the log file gives detailed information about the transactions previous to that
error.
10.1 Chemical Problems

Chemical problems may appear with an error message along with the results, or by unexpected
results for the processed samples. They may appear in the following situations:
- Calibration errors
- Alarms with control or patient results
- Quality control results outside the defined ranges
- Unexpected patient results
From the results obtained from the method calibration, quality controls, and patient samples tested,
decide which of the following conditions best describe the problem encountered and run the checks
and actions associated with each case:
- High results
- Low results
- Erratic results
- Only one affected sample for all the methods
- Only one affected method for all the samples
10.1.1 High results

10-1
Observation Probable cause Action
Incubation temperature > High cuvette tray temperature Check temperature offset
37ºC (heater and preheater).
Heater and preheater
operation.
Thermal switch (N/C)
High ambient temperatura Reduce the ambient temp to <
25 ºC
Low calibrator absorbance Incorrect preparation of the Use a calibrator reconstituted
results calibrator dilution correctly
Presence of bubbles or fibrin in Use a calibrator free of bubbles

the calibrator tube
High blank reagent absorbance Verify the blank reagent
Insufficient calibrator volume Use a minimum volume of 200
µl
Samples concentrated by Insufficient sample and control Use a minimum volume of 200
evaporation volumes µl
Inadequate sample and control Use fresh samples and controls
storage
Deteriorated reagent Inadequate reagent preparation Prepare new reagent
Refilled reagent not mixed well, Mix the reagent gently and
or not checked verify the blank
Physical and/or chemical Prepare new reagent

deterioration of the reagent
Incorrect calibrator Calibrator setpoint is incorrect Verify calibrator setpoint
setpoint
Incorrect aspiration or Losses in the diluter circuit Adjust connections and purge
dispensing of samples and connections the diluter
reagents Obstruction of the TIP Purge the diluter
Run a TIP cleaning
Bubbles in the tubing or diluter Adjust connections and run a
TIP cleaning
Insufficient reagent Reagent volume less than 2 ml or Refill the reagent bottle or
volume or bubbles into more than 55 ml change it without any bubbles
the reagent bottle Inadequate purge of the Purge the diluter or wash
hydraulic system cuvettes
10.1.2 Low Results

10-2
Incubation temperature < Low cuvette tray temperature Check temperature offset
37ºC (heater and preheater).
operation.
Low ambient temperature Increase the ambient temp to >
15 ºC
High calibrator absorbance Incorrect preparation of the Use a calibrator reconstituted
results calibrator dilution correctly
Low blank reagent absorbance Verify the blank reagent

Inadequate samples Insufficient sample and control Use a minimum volume of 200
volumes µl
Presence of bubbles or fibrin in Use samples free of bubbles
the sample tubes and/or fibrin
Absence of the sample in the Place the samples in the correct
assigned position positions
Refilled reagent not well mixed, Mix the reagent gently and
or not checked verify the blank
Physical and/or chemical Prepare new reagent and verify

deterioration of the reagent the blank
Incorrect calibrator Calibrator setpoint is incorrect Verify calibrator setpoint
setpoint
reagents Obstruction of the TIP Purge the diluter
Run a TIP cleaning
TIP cleaning
Reagent volume less than 2 ml or Refill the reagent bottle or
more than 55 ml change it without any bubbles
10.1.3 Erratic Results

Incorrect information in Incorrect type of reaction chosen Select the correct type of
the method configuration for each method reaction

10-3
Method parameters configured Configure the parameters
incorrectly adequately
Incorrect calibration Calibrator concentration Configure the calibrator cc
configured incorrectly correctly
Inadequate samples Sample volumes less than 200 µl Use a minimum volume of 200
µl
Presence of bubbles or fibrin in Use samples free of bubbles
the sample tubes and/or fibrin
Absence of the sample in the Place the samples in the correct
assigned position positions
Presence of air in the Incorrect purge of the diluter Purge the diluter
hydraulic system circuit
Insufficient washing solution Refill the washing solution and

purge the hydraulic system
completely
Reaction cuvettes Program session closed and loss Wash all the cuvettes
assigned incorrectly of the cuvettes current state
Dirty cuvettes Maintenance program did not Run the remaining maintenance
finish completely operations
Contamination of the cuvettes Run a special wash cycle with
NaOH
0.2 N
Failure of the washing and/or Check Pump operation.
drying of the cuvettes Check washer header height.
Check dryer height.
Failure of the optical Burned lamp Replace the lamp
system Deteriorated cuvettes Replace the cuvettes
Inadequate calibration of the Calibrate the photometer
optical system Calibrate the cuvettes
Contamination of the Prolonged non-use of the Run a decontamination process
circuits instrument
Erratic incubation Erratic cuvette tray temperature Check temperature offset
temperature (heater and preheater).
operation.
reagents Obstruction of the probe and/or Purge the diluter and run a TIP
of the pre-heater cleaning cycle
TIP cleaning
Reagent volume less than 2 ml or Refill the reagent bottle or
more than 55 ml change it without any bubbles

10-4
10.1.4 Only one affected sample for all of the methods

Incorrect sample position Sample incorrectly positioned Place the sample in the correct
position
Incorrect control Incorrect control range Configure the control ranges
configuration assignment correctly
Incorrect selection of Selection of methods incorrect Select the correct methods for
methods for the type of sample the type of sample
Incorrect preparation of Sample reconstituted with an Reconstitute a new vial
the sample inadequate volume or poorly correctly
homogenized
Sample diluted incorrectly Dilute the sample correctly
Inadequate sample Insufficient volume Use a minimum volume of 200
integrity µl
Inadequate storage Use fresh or well preserved
samples
Presence of bubbles and/or fibrin Use a sample free of bubbles
in the sample tubes and fibrin
Presence of sample Use a new sample free of
interferences: hemolysis, interferences
jaundice, lipemic specimen,
turbidity
10.1.5 Only one affected method for all of the samples

Insufficient reagent Reagent volume less than 2 ml or Refill the reagent bottle or
volume or bubbles into more than 55 ml change it without any bubbles
the reagent bottle
Inadequate reagent Incorrect positioning of the Assign the reagent position
reagent in the tray correctly
Inadequate reagent container Use only the reagent bottles
designed for the instrument
Dirty reagent bottles Wash the reagent bottles
periodically
Insufficient reagent volume Refill the reagent bottle
Refilled reagent not Mix the reagent gently and
homogenized, or not verified verify the blank
Avoid foaming
Physical and/or chemical Prepare new reagent and verify
deterioration of the reagent the blank

10-5
Incorrect information in Incorrect type of reaction chosen Select the correct type of
the configuration for the method reaction
Method parameters configured Configure the parameters
incorrectly correctly
Incorrect assignment of the Configure the controls range
control range correctly
Inadequate calibration Calibrator setpoint is incorrect Verify calibrator setpoint
Incorrect preparation of the Use a calibrator reconstituted
calibrator correctly
Incorrect reagent blank Verify the blank reagent
absorbance
Incorrect calibrator absorbance Calibrate the method
Message of a re-diluted Linearity configured incorrectly Configure the linearity
result or re-dilute correctly: a value between 0.80
manually with results y 0.95
within the linear limit Initial consumption configured Configure the method’s initial
incorrectly consumption correctly
10.2 Instrumental Problems
Instrumental problems may appear with visible failures in its components or by malfunction errors
that show up in the program. Instrument problems may often be the cause of observed reagent
problems. (See point 10.4)
Based on these observations, instrumental problems can be grouped according to the affected
module or component of the instrument. Decide which of the following components is probably
affected and run the associated checks and actions in each case:
- Connections and supplies

- Hydraulic system
- Diluter
- Sample/Reagent probe
- Reaction cuvette tray
- Optical system
- Software
- ISE Module (Optional)
If this does not solve the problem, contact a Technical Support.
10.2.1 Connections and supplies

Failure in the start up of Instrument disconnected Plug the cable into the power
the instrument source
Blown start up fuse Replace the fuses

10-6
No electricity from the power Reestablish the electricity
source
Damaged start-up button Replace switch.
Failure in the PC- Instrument turned off Turn on the instrument
instrument Instrument turned off and on Close and open the program
communication without closing the program again
Invalid serial port Close all open applications that
might be using the serial port
and re-open the program
PC disconnected from the Connect the PC-instrument
instrument cable RS232C
10.2.2 Hydraulic system

Presence of bubbles/air in Connections adjusted incorrectly Adjust the connections
the tubing Purge the diluter
Partial obstruction of the probe Purge the diluter
Run a TIP cleaning cycle
PTFE on the diluter worn out Contact Technical Support
Incorrect purge of the diluter Purge the diluter
circuit
Insufficient wash solution Refill the wash solution and
purge the diluter five times and
wash cuvettes
Worn out hose Replace the peristaltic pump
hose
Dripping peristaltic pump Drain hose bent or obstructed Straighten or un-obstruct the
hose drain hose
Overflowing of the probe Possible bottleneck or siphon Stretch the drain hose and check
wash station effect in drain hose for any obstructions
Presence of water under
the instrument
10.2.3 Diluter

Presence of bubbles or air Connections adjusted Adjust the connections
in the diluter syringe inadequately Purge the diluter
Damaged tubing Contact Technical Support
Blocked probe Run a TIP cleaning cycle
Purge the diluter

10-7
Dripping diluter syringe PTFE on the diluter worn out Contact Technical Support
Diluter syringe body cracked or Contact Technical Support
worn out
Error message: Diluter Reaction volumes configured at > Configure the method correctly
error (after processing a 500 µl
particular method)
10.2.4 Sample/reagent probe

Dripping probe Blocked probe Run a TIP cleaning cycle
Purge the diluter
Pre-heater failure Contact Technical Support
Faulty connections Contact Technical Support
Incomplete movement: Liquid detection failure Clean the probe tips with paper
the probe does not lower towel dipped in ethyl alcohol
to aspirate samples and/or Purge the diluter
reagents Poor quality water Use good-quality distilled water
Error message: Probe Mono-reagent bottles in the Place the reagent bottles
collision (Collision of the position of the bi-reagent bottles correctly
probe in its vertical
movement) Blocked sample tube Unblock the sample tubes
Probe collision with the Failure of liquid detection Clean the probe tips with paper
bottom of a sample tube towel dipped in ethyl alcohol
or reagent bottle Purge the diluter
Error message: Dispensing Presence of obstacles in the way Remove obstacle or correct the
Arm Collision of the dispensing arm cause of the collision
(Collision of the probe in
its horizontal movement)
Unusual noise with Presence of bubbles between the Clean the probe tips with paper
upward movement two ends of the probe towel dipped in ethyl alcohol
Purge the diluter
Damaged probe: Collision of the probe Contact Technical Support
aspiration tip and sensor
tip of the same length
Probe and its capillary Adhesion of remains of samples Clean the exterior of the probe
dirty and/or reagents with paper towel dipped in
ethyl alcohol
Purge the diluter

10-8
10.2.5 Reaction Cuvettes Plate

Formation of crystals in Incomplete run of the wash Run an external wash and
the upper part of the program calibration of the cuvettes
cuvettes
Presence of water on the Inadequate purge of the washer Purge the washer 5 times
cuvette strips Insufficient washing solution Refill the washing solution
Presence of bubbles in the container and purge the diluter
cuvettes during the wash and washer five times
Air entering the tubing Check the connection of the
hoses to the washing solution
container
Wash cuvettes
Damaged/opaque Prolonged use Change the cuvettes and
cuvettes calibrate them
Cuvettes still dirty after Washer failure Contact Technical Support
washing
10.2.6 Optical system

Error message: Cover Cuvettes tray cover left open Replace cuvettes tray cover and
open/Burned Lamp during the reading of the reaction restart the operation
Lamp burnt out Change the lamp
Lamp turned off Contact Technical Support
Error message: Cuvette Cuvettes tray cover left open Replace cuvette tray cover and
Calibration ended with during the calibration restart the calibration
errors
Damaged cuvettes Change the reaction cuvettes
Damaged filter Contact Technical Support
Washer failure Contact Technical Support
10.2.7 Software

Error message: Processing Reaction reading cannot be 1) If the flag appears after stop
error # Process xxxxxxxxxxx taken in the specified time (Time procedure, you do not have to

10-9
Retry/Cancel out) take any action. Flag appears
Time Out. Incubation time because of that.
has expired. It will be 2) If the flag appears at random
automatically repeated. time without any reason, check
data base size and computer
characteristics.
3) If the flag appears repeated
times in the same methods
check that methods settings.
Results expressed as a Nan Error in the calculation of the Verify methods settings, verify
(not a number) calibration or the sample result reagent and recalibrate the
method
Error message: The Mechanical or communication Contact Technical Support

instrument cannot be failure
initialized
10.3 Malfunction problems displayed on the screen

Error messages will be displayed on the screen like this:
Figure 10-1
These kinds of messages report the Error ID number and a brief description of the problem.
Below, there’s a list with all the Hardware Messages, including a probable cause and a possible
solution.
NOTE: This tool is useful in most cases, but it is not a definite solution for all the problems.
For those cases where the user can’t remember the information shown in the error messages
there’s a tool that technical service can go to search the errors, this tool is the log.html file. The
log.html files are described in the next section.

10-10
HARDWARE MESSAGES
10.3.1 GENERAL
Error ID Number and Description Probable cause Possible Solution
(0001) The Analyzer has been Powered off or reset Initialization sequence
powered on. An initialization of Autoanalizer
sequence will be required
(0002) A general power down The Analyzer general Long term general power interruptions
happened to the Analyzer, it could power has been will affect the ISE electrodes life
affect the ISE module interrupted. expectancy and performance. Avoid
performance. Please purge, using the general power key. Purge,
calibrate and control it calibrate and control the ISE module.
(0003) Low Reagent Volume Non reagent Necessary loading of reagent
remaining
(0004) Check EEPROM version Main controller Consult to factory for actualization
actualization
(0005) Slave Communication error Error by slave Restart autoanalizer. If the same
controller problem stays, change Controller PCB.
communication
(0006) Error during reaction Failure in level Verification for setting offset in vertical
mixing detection during movement by dispensed position
homogenization. (eeprom 32).
Verify cuvettes volume
(0007) Maintenance Level 1 must Cycles/hours were Consult "MAINTENANCE BASED ON

be done. Contact Technical achieved for level 1 ALARMS" section
Support maintenance
Support maintenance
Support maintenance
(0010) Lamp rated lifespan Cycles/hours for using Preventive change of the lamp
reached. Contact Technical lamp were achieved
Support
10.3.2 EEPROM
Error ID Number and Probable cause Possible Solution
Description

10-11
(0031) EEPROM writing Wrong direction or Verify direction and type of argument. Replace
error defective EEPROM EEPROM in case the failure goes on.
(0032) EEPROM reading Wrong direction or Verify direction. Replace EEPROM in case the
error defective EEPROM failure goes on.
(0033)EEPROM writing Enabling command Execute the command "eepromWriteEnable A1
disabled not executed 1" from the command console (Terminal line).
(0034) EEPROM erasing Defective EEPROM Verify the existence of EEPROM U24.
error In case the failure goes on, replace EEPROM.
In case the failure goes on, replace PCB

Controller.
(0035)EEPROM absorbance Defective EEPROM Verify the existence of EEPROM U24.
clearing error In case the failure goes on, replace EEPROM.
In case the failure goes on, replace PCB

Controller.
(0036 Wrong Direction EEPROM error direction. Check the direction
EEPROM writing error value
(variable does not exist)
(0037) Wrong Direction EEPROM error direction. Check the direction

EEPROM reading error value.
(variable does not exist)
10.3.3 VERTICAL
Description
(0101) Physical impact Verify possible obstacles along the path of header
Probe Impact (Vertical movement.
Movement) Impact switches Verify the proper working of the switches and
failures springs in preheater board.
(preheater board)
Poor contacts Check the pins on J3 of PCB preheater and on J24
of PCB Controller.
(0102 Home sensing Verify pins in J6 of PCB Controller and in J1 of the
Probe Vertical Movement Failure PCB Sensor R (Vertical) of robot module Robot.
not initialized or initialization Then verify electrical contact pin to pin.
error (Home active)
Verify the proper working of the PCB Sensor R
(Vertical).

10-12
Verify the proper working of PCB Controller. To do
this, measure with a voltmeter on pin 9 of IC U30,
ensuring the switching of the logic state when
blocking and unblocking sensor R (vertical)
Mechanical Verify belt

failure Verify the stepper motor movement (When
initializing vertical movement, if the flag is
blocking the vertical Sensor, the stepper motor
must go down 60 steps)
Vertical Execute commands of initialization movement.
movement not
initialized.
(0103) Sensing failure Verify pins in J6 of PCB Controller and in J1 of the
Probe Vertical Movement home PCB Sensor R (Vertical) of robot module. Then,
not initialized or initialization verify electrical contact pin to pin.
error (Home inactive)
Verify the proper working of the PCB Sensor R
(Vertical).
Mechanical Verify belt

failure Verify the stepper motor movement (if starting
the vertical, the vertical flag is not blocking the
sensor, the stepper motor must go up until HOME
position
Vertical Execute commands of initialization movement
movement not
initialized
(0104) Home sensing Verify pins in J6 of PCB controller and in J1 of the

Probe Vertical Movement failure PCB sensor R (vertical) of the robot module. Then,
error (Home not found) verify the electrical contact pin to pin.
Verify the proper working of the PCB sensor R

(vertical)
Mechanical Verify belts.

10-13
failure. Verify the stepper motor upward movement
Verify that the flag obstructs sensor R (vertical)
(0105) probe vertical Home sensing Verify pins in J6 of PCB controller and J1 of the
movement error (home failure PCB sensor R (vertical) of robot module. Then,
active) verify electrical contact pin to pin.
Verify the proper working of the PCB sensor R.

Mechanical Verify belt.

failure Verify the stepper motor downward movement
Verify the flag unblock sensor R (vertical)
(0106) Controller old Contact the company to update controller.

Upgrade Vertical Movement version.
Controller
(0107) probe level not Wiring failure Check electrical contact between J3 of PCB
detected(level inactive) preheater and J24 of PCB controller.
Probe failure. Verify electrical contact between probe auxiliary
capillary and pin 2 in J1 of PCB preheater and
between main capillary tube (for dispense) and
the pin 1 on J1 of PCB preheater.
Preheater PCB Replace the PCB Preheater

failure
(0108) See error solving 101 and 109.
Probe Impact (Vertical
Movement) and incorrect
Probe Level Detection (Level
active)
(0109 Sensing failure Clean the probe in case of possible dirtiness or a
Incorrect Probe Level drop between the auxiliary and the main capillary
Detection (Level active) Probe failure Check the insulation between the main capillary
and the auxiliary: with a multimeter at the range
of 20Mohms must measure high impedance

10-14
Module Verify the insulation of the module. NOTE: the
preheater failure stainless capillary in the module must be insulated
from the Power Resistance and chassis that hold
them.
Wiring failure Check the contact between J3 of PCB preheater
and J24 of PCB controller.
10.3.4 HORIZONTAL
Description
(0151)probe impact Physical impact Verify possible obstacles in the movement
(horizontal movement)
Impact switches Verify the proper working of the switches and
failures (preheater springs in the preheater board.
board)
Poor contacts Check the pins on J3 of PCB preheater and J24
of PCB controller.
(0152) Home Sensing Verify pins on J4 of PCB controller and J1 of
Probe Vertical Movement failure PCB sensor R (horizontal) of robot module.
not initialized or initialization Verify the proper working of the PCB sensor R
error (Home active) (horizontal)
Verify the proper working of the PCB
controller. To do this, measure with a
voltmeter pin 9 of IC U33 the switching of the
logic states when blocking and unblocking
sensor R (horizontal)
Mechanical failure Verify belt.
Verify stepper motor movement (when
initializing horizontal movement, if the arm is
blocking horizontal sensor ,this should move
230 clockwise)
Horizontal Execute the commands to initialize the
movement not movement
initialized
(0153) Home sensing Verify pins on J4 of PCB controller and J1of PCB
Probe Vertical Movement failure sensor R (horizontal) of robot module. Then,
not initialized or initialization verify electrical contact pin to pin.
error (Home inactive) Verify the proper working of the PCB Sensor R
(Horizontal).

10-15
Verify the proper working of PCB controller. To
do this, measure with a voltmeter pin 9 of IC
U33, ensuring the switching of the logic states
when blocking and unblocking sensor R
(horizontal).
Verify stepper motor movement (when

initializing horizontal movement, if the
horizontal flag is not blocking the sensor, the
stepper motor should counter-clockwise until
HOME position)
Horizontal Execute the commands to initialize the
movement not movement
initialized
(0154) Home sensing Verify pins on J4 of PCB controller and J1 of
Probe Horizontal Movement failure PCB sensor R (horizontal) of robot module.
error (Home active) Then verify electrical contact pin to pin.
Verify the proper working of PCB sensor R
(horizontal)
(horizontal).
Mechanical failure Verify belt
Verify the horizontal movement of the arm

when it rotates clockwise.
Verify that the flag unblocks the sensor R
(horizontal).
(0155) Home sensing Verify pins on J4 of PCB

Probe Horizontal Movement failure
error (Home inactive) Verify the proper working of PCB sensor R
(horizontal).
(horizontal).
Verify the horizontal movement of the arm

when it rotates counter-clockwise.

10-16
Verify that the flag unblocks sensor R
(horizontal).
10.3.5 Reaction tray

Error ID Number Probable Possible Solution
and Description cause
(0201) Physical Verify possible obstacles in the movement.
Probe Impact impact
(Reaction Tray)
Impact Verify the proper working of the switches and springs in preheater
switches board.
failure
(preheater
board)
poor Check pins J3of PCB preheater and J24 of PCB controller.
contacts
(0202) Home Sensor P switched off.
Reaction Tray not sensing Verify pins J12 of PCB controller and J1 of PCB sensor P of reaction
initialized or failure tray. Then, verify electrical contact pin to pin.
initialization error
(Home active)
Verify the proper working of PCB sensor P (optical sensor)
Verify the proper working of PCB controller. To do this, measure with a

voltmeter pin 9 of IC U9, ensuring the switching of the logic states
when blocking and unblocking sensor P by means of the slots of the
slotted-wheel.

failure Verify stepper motor movement to execute commands of movement
or initialization.
Reaction Execute commands to initialize module.
tray not
initialized
(0203) Home Verify pins J12 of PCB controller and J1 of PCN sensor P of reaction
Reaction Tray not sensing tray. Then, verify electrical contact pin to pin.
initialized or failure.

10-17
initialization error Verify the proper working of PCB sensor P (optical sensor)
(Home inactive)
slotted-wheel.

failure Verify the stepper motor movement when executing commands of
initialization.
Reaction Execute command of module initialization.
tray not
initialized.
(0204) Module not
Reaction Tray not initialized.
initialized
(0205) Home Verify pins on J12 of PCB controller and J1 of PCB sensor P of reaction
Reaction Tray sensing tray. Then verify electrical contact pin to pin.
error (slotted tray error.
counting error)
Verify the proper working of PCB sensor P (optical sensor)

slotted-wheel.
Mechanical Verify there’s nothing blocking the reaction tray movement and the
failure. stepper motor does not lose any steps
Verify belt and tension

Sensor P out Adjust the sheet (the one in shape of “L”) supporting sensor P, so that,
of counting it is positioned to be blocked and unblocked by means of the slots of
position. the slotted-wheel (radial position).
NOTE: make sure there is not any friction between the slotted-wheel
and the optical sensor.
(0206 Mechanical Verify belt and tension.

Reaction Tray failure

10-18
error (Home Sensing Adjust the sheet (the one in shape of “L”) supporting sensor P, so that,
active) failure it is positioned to be blocked and unblocked by means of the slots of
the slotted-wheel (radial position).
Verify pins J12 of PCB controller and J1 of PCB sensor P of reaction

tray. Then, verify electrical contact pin to pin.
(0207) Mechanical Verify belt and tension.

Reaction Tray failure.
error (Home Sensing Adjust the sheet (the one in shape of “L”) supporting sensor P, so that,
inactive) failure. it is positioned to be blocked and unblocked by means of the slots of
the slotted-wheel (radial position).
Verify pins J12 of PCB controller and J1 of PCB sensor P of reaction

tray. Then, verify electrical contact pin to pin
(0208) Mechanical Verify belt of washer module

Reaction Tray failure. Verify there’s nothing blocking the reaction tray movement and the
movement stepper motor does not lose any steps
inadequate safety
conditions Sensing Verify the proper working of PCB sensor L (two optical sensors)
(Washer) failure of W-
UP and W- Verify pins on J8 of PCB controller and J1 of PCB sensor L of washer
DOWN. module. Then, verify electrical contact pin to pin.

voltmeter on pin 9 and 11 of IC U26, ensuring the switching of the logic
states when blocking and unblocking sensor L during the washer
movement.
NOTE: the equipment verifies the state of the washer before moving the reaction
tray.
(O209) Same values Increase or decrease the dispensed offset value. Add or subtract 1 in
Reaction Tray for the direction 82 of EEPROM.
programming dispensed
error (Dispensing offset and

10-19
and Photometer photometer
offset) offset.
10.3.6 FILTER WHEEL

Description
(0302) Home sensing Sensor F disconnected.
Filter Wheel not failure. Verify pins on J10 of PCB controller and J1 of PCB sensor F of
initialized or photometer. Then, check the electrical contact pin to pin.
(Home active)
Verify the proper working of PCB sensor F (optical sensor).
Verify the proper working of PCB. To do this, measure with a

voltmeter on pin 9 of IC U13, the switching of the logic
states when blocking and unblocking sensor F by means of
the slotted-wheel movement.
Mechanical Check the suitable attach between the filter wheel and the
failure. stepper motor axis.
Verify stepper motor movement of the filter wheel when
executing commands of movement or initialization.
Filter wheel not Execute commands to initialize the module.

initialized.
(0303) Home sensing Verify pins on J10 of PCB controller and J1 of PCB sensor F of
Filter Wheel not failure photometer. Then, check electrical contact pin to pin.
initialized or
(Home inactive) Verify the proper working of PCB controller of PCB sensor F
(optical sensor).
Verify the proper working of PCB controller. To do this,
measure with a voltmeter on pin 9 of IC U13, the switching
of the logic states when blocking and unblocking sensor F by
means of the slotted-wheel movement.
Mechanical Check the suitable attach between the filter wheel and the
failure stepper motor axis

10-20
Verify the filter wheel stepper motor movement when
executing commands of movement or initialization.
Filter wheel not Execute commands to initialize the module.

initialized.
(0304) Module not Initialize module
Filter Wheel not initialized.
initialized
(0305) Home sensing Verify pins on J10 of PCB controller and J1 of PCB sensor F of
Filter Wheel error failure. photometer. Then, check electrical contact pin to pin.
(slotted tray counting
error)
Verify the proper working of PCB sensor F (optical sensor)
Verify the proper working of PCB controller. To do this,

measure with a voltmeter on pin 9 of IC U13, the switching
of the logic states when blocking and unblocking sensor F by
means of the slotted-wheel movement.
Mechanical Verify that the stepper motor does not lose any step and
failure there’s nothing blocking the filter wheel movement. Check
all filters are properly screwed.
Sensor F out of Adjust the sheet supporting sensor F, so that, it is

the counting positioned to be blocked and unblocked with the slots of
position. the slotted-wheel (That belong to the filter wheel)(radial
position). NOTE: make sure that there is not any friction
between the slotted-wheel and the optical sensor.
(0306) Poor contacts. Verify connector J9 (stepper motor)

Filter Wheel error Mechanical Verify that the stepper motor does not lose any step and
(Home active) failure that no lock is found in the filter wheel movement. Check
that all filters are well screwed.
Sensing failure Adjust the sheet supporting sensor F, so that it is positioned

to be blocked and unblocked with the slot of the filter wheel
(radial position). NOTE: make sure that there is not any
friction between the slotted-wheel with the optical sensor
Verify pins J10 of PCB controller and J1 of PCB sensor F of

photometer. Then, check electrical contact pin to pin.
(0307) Poor contacts Verify connector J9 (stepper motor).

10-21
Filter Wheel error Mechanical Verify that the stepper motor does not lose any step and
(Home inactive) failure that no lock is found in the filter wheel movement. Check
that all filters are well screwed.
Sensing failure. Adjust the sheet supporting sensor F, so that it is positioned

to be blocked and unblocked with the slot of the slotted-
wheel (radial position). NOTE: make sure that there is not
any friction between the slotted-wheel with the optical
sensor
Verify pins J10 of PCB controller and J1 of PCB sensor F of

photometer. Then, check electrical contact pin to pin
10.3.7 READING (AMPLIFIERS)

Description
(0321 A reading was performed Wait a reasonable time.
The time is not enough before reaching the time of (it is recommended at least 10 minutes)
for thermal stabilization. thermal stabilization.
Please repeat the test
after some minutes
(0322) It has been intended to carry Place the cover of the reaction tray.
Reaction Tray uncovered. out a reading operation
Please place the cover without placing the cover of
the reaction tray.
Light sensor failure. Check the working of the sensor and the
sensing circuit of PCB controller.
(0323) Lamp burned out. Replace the lamp.
Lamp error (burned,
unconnected or power
failure)
Lamp disconnected or Check lamp connections. Measure the
without any supplies. contact from both sides of the wire
(orange and yellow)
Measure the voltage on its connector
and J2 of PCB regulator, using a
multimeter.
(0324) The lamp turned off and the Execute commands: “IH A1” to turn on
Lamp powered off analyzer attempts to read. the lamp and do the reading again.

10-22
(0325) Communication error If the problem goes on, try to replace the
Internal CRC error between amplifier controller amplifier controller. If the problem
(Amplifier) and Main controller. remains, contact the manufacturer.
(0326) Lack of calibration. Run photometer calibration.

Low energy in Reference Exit Lens of the reference Verify the lens is clean
Beam. Calibrate channel
Photometer Defective Lamp After trying with the alternatives above;
make sure the lamps have not finished
the lifecycle. Contact the manufacture to
know about cases in which it should be
changed.
Defective Beam Filter Check the filter
(0327) Lack of calibration. Do photometer calibration.
Low energy in Sample Scratch Cuvette or not Check the cuvettes alignment with the
Beam. Check Sample aligned. beam
absorbance Replace the cuvette or try to calibrate
another cuvette. (it is recommended to
change the defective cuvettes)
Exit Lens of the reference Verify the lens is clean
channel
Defective Lamp After trying with the alternatives above;
make sure the lamps have not finished
the lifecycle. Contact the manufacture to
know about cases in which it should be
changed.
Defective Beam Filter Check the filter
(0328) Reaction tray uncover Place the cover of the reaction tray.
Saturated Reference
Amplifier Reference amplifier Check the reference amplifier is properly
disconnected. connected.
Reference amplifier failure. Once the wiring is properly checked, if
the problem remains, try replacing the
amplifier board.
Filter missing. Verify the filter wheel has no missing
filter.
Incorrect positioning of a Check that the filters are in the correct
Beam Filter. order. Note: the correct order is 340,
380, 405, 450, 505, 546, 578, 600, 650,
700, OPTIONAL, Stopper-Beam.
(0329) Reaction tray uncovered Place the cover of the reaction tray.
Saturated Sample
Amplifier Reference amplifier Check the reference amplifier is properly
disconnected connected.

10-23
(0329) Reference amplifier failure. Once the wiring is properly checked, if
Saturated Sample the problem remains, try replacing the
Amplifier amplifier board.
Filter missing Verify the filter wheel is completed.

380, 405, 450, 505, 546, 578, 600, 650,
Amplifiers disconnected. Check the amplifiers are properly
connected.
Filter missing Verify the filter wheel is completed

380, 405, 450, 505, 546, 578, 600, 650,
(0330) Lamp failure Measure the voltage on the lamp
High energy Reference connector; verify it’s within the range of
Beam 12Volts +/- 0.1Volt
380, 405, 450, 505, 546, 578, 600, 650,
Defective filter Replace the beam filter
(0331) High energy Lamp failure Measure the voltage on the lamp
Sample Beam connector; verify it’s within the range of
12Volts +/- 0.1Volt
380, 405, 450, 505, 546, 578, 600, 650,
Defective filter Filter change
(0332) Reaction tray lid not placed. Place the lid of the reaction tray.
Saturated Sample and
Reference Amplifiers Amplifiers disconnected. Check the amplifiers are properly
connected.
Filter missing Verify the filter wheel is completed

380, 405, 450, 505, 546, 578, 600, 650,

10-24
10.3.8 PHOTOMETER CALIBRATION
Description
(0351) Filter ID values set Check that the filters are properly set in the
Photometer Calibration error on the EEPROM EEPROM.
(no filters programmed in
EEPROM)
(0352) Possible failure in Check the working of the IC controller. For

Photometer Calibration error filter wheel more information contact the manufacturer.
(Filter Wheel Controller controller.
communication)
(0353) Possible failure in Check the working of the IC controller. For

Photometer Calibration error amplifier IC more information contact the manufacturer
(Amplifier Controller controller.
communication)
(0354 Calibration logic Contact the manufacturer

Photometer Calibration error error.
(0355) Scratched cuvette Check the cuvette alignment with the beam.
Photometer Calibration error or not aligned.
(low energy in Sample Beam) Replace the cuvette or try to calibrate
another cuvette. (Anyway, it is recommended
to replace defective cuvettes)
Exit lens of the Verify the lens is clean
reference channel.
Amplifier sample Once the wiring is properly checked, if the
Failure. problem remains, try replacing the amplifier
board.
(0356) Exit lens of the Verify the lens is clean
Photometer Calibration error reference channel.
(low energy in Reference Beam) Failure in the Once the wiring is properly checked, if the
amplifier reference. problem remains, try replacing the amplifier
board.

10-25
(0357 Defective lamp Make sure the lamps have not finished the
Photometer Calibration error lifecycle. Contact the manufacture to know
(low energy in Sample and about the cases in which it should be
Reference Beam) changed.
Exit lens. Verify the lens is clean
Incorrect Check that the filters are in the correct order.

positioning of a Note: the correct order is 340, 380, 405, 450,
Beam Filter. 505, 546, 578, 600, 650, 700, OPTIONAL,
Black Stopper
Defective filter Check the integrity of the filter.
(0358) Alignment failure in Check the alignment of the cuvette with the
Photometer Calibration error the optical path beam.
(high energy in Sample Beam) Sample Amplifier Once the wiring is properly checked, if the
board.
(0359) Reference Amplifier Once the wiring is properly checked, if the
Photometer Calibration error Failure. problem remains, try replacing the amplifier
(high energy in Reference board.
Beam)
(0360) Incorrect Check that the filters are in the correct order.
Photometer Calibration error positioning of a Note: the correct order is 340, 380, 405, 450,
(high energy in Sample and Beam Filter. 505, 546, 578, 600, 650, 700, OPTIONAL,
Reference Beam) Black Stopper
Lamp failure Measure tension on the connector using a
voltmeter, it should be 12V +/- 0.1V
Defective filter Filter change
Amplifiers failure Check the amplifier connection (they should
not be inverted)
(0361) Photometer Calibration Spurious light. Check the photometer is properly covered
error (high Zero in Sample and there is no light coming from outside.
Amplifier)
Sample Amplifier Once the wiring is properly checked, if the
board.

10-26
(0362) Photometer Calibration Spurious light. Check the photometer is properly closed and
error (high Zero in Reference no light is reached.
Amplifier)
Reference Amplifier Once the wiring is properly checked, if the
board.
(0363) Photometer Calibration Incorrect Check that the filters are in the correct order.
error (high Zero in Sample and positioning of a Note: the correct order is 340, 380, 405, 450,
Reference Amplifiers) Beam Filter. 505, 546, 578, 600, 650, 700, OPTIONAL,
Black Stopper
Lamp failure Measure tension on the connector using a
voltmeter, it should be 12V +/- 0.1V
Defective filter Replace the filter
Missing filter Check the filter wheel is completed.
Amplifiers failures Check the amplifier connection (they should

not be inverted)
(0364) Photometer Calibration Defective EEPROM Replace EEPROM. Contact the manufacturer.
error (EEPROM writing error)
(0365) Photometer Calibration Dirty cuvette Clean the cuvette

(High Gain Sample Amplifier) Sample lens Turning dirtiness over in sample lens
obstructed
Failure problem remains, try replacing the amplifier
board.
(0366) Photometer Calibration Reference lens Remove dirtiness in reference lens
(High Gain Reference Amplifier) obstructed
Failure problem remains, try replacing the amplifier
board.
(0367) Photometer Calibration Cycles/hours for Preventive change of the lamp
(High Gain Sample and using lamp were
Reference Amplifiers) achieved
Input lens or beam Remove dirtiness on lens or beam splitter
splitter obstructed
Lamp power fell Measure tension on the connector using a
down voltmeter, it should be 12V +/- 0.1V

10-27
10.3.9 Cuvettes calibration.

Description
(0381) Filter ID values set on Check that the filters are properly set in the
Cuvettes Calibration error the EEPROM EEPROM.
(no filters programmed in
EEPROM)
(0382) Possible failure in IC Check the working of the IC controller. For

Cuvettes Calibration error filter-wheel controller. more information contact the manufacturer
(Filter Wheel Controller
communication)
(0383) Possible failure in IC Check the working of the IC controller. For

Cuvettes Calibration error controller (Amplifier). more information contact the manufacturer
(Amplifier Controller
communication)
(0384) Error in the logic of For more information contact the

Cuvettes Calibration error calibration. manufacturer
(0385) Lack of calibration. Run photometer calibration.

Cuvettes Calibration error Scratched cuvette or Check the alignment of the cuvettes with the
(low energy in Sample poorly aligned. beam.
Beam) Replace the cuvette.
(it is recommended to replace the defective
cuvettes)
Exit lens of the sample Check the lens is clear.
channel
(0386) Lack of calibration. Do photometer calibration.
Cuvettes Calibration error Exit lens of the Check the lens is clear.
(low energy in Reference reference channel.
Beam)

10-28
(0387) Scratched cuvette or Check the alignment of the cuvette with the
Cuvettes Calibration error poorly aligned. beam.
(Cuvette absorbance too
high, replace the cuvettes)
Replace the cuvette.

(it is recommended to replace the defective
cuvettes)
Lack of calibration Do photometer calibration
(0388) Lack of calibration Do photometer calibration
Cuvettes Calibration error Cuvette not aligned. Check the alignment of the beam with the
(Cuvette absorbance too cuvette.
low, Calibrate Photometer Replace the cuvette.
in a different cuvette or (it is recommended to replace the defective
replace de cuvettes) cuvettes)
(0389) Striped cuvette or Check the alignment of the beam with the
Cuvettes Calibration misaligned cuvette.
(Cuvette absorbance too Replace the cuvette.
high, replace the cuvettes (it is recommended to replace the defective
when possible) cuvettes)
Lack of calibration Run photometer calibration
(0390) Lack of calibration Run photometer calibration
Cuvettes Calibration Cuvette poorly aligned. Check the alignment of the cuvette with the
(Cuvette absorbance too beam.
low, Calibrate Photometer Replace the cuvette.
in a different cuvette or (it is recommended to replace the defective
replace de cuvettes when cuvettes)
possible)
(0391) Defective EEPROM. Replace EEPROM.

Cuvettes Calibration error Contact the manufacturer.
(EEPROM writing error)

10-29
10.3.10 HEATER AND PRE-HEATER
Error ID Number and Probable Possible Solution
Description cause
(0401) Wiring Check pin to pin the electrical contact of the wiring
Temperature reading error failure that connects J16 of PCB controller and J1 of PCB
(can't read Reaction Tray A/D Heater.
converter) A/D Once the wire is checked, try to replace the Heater
defective PCB. (Caution: avoid handling these boards without
the electrostatic bracelet)
(0451) Wiring Check pin to pin the contact of the wiring that
Temperature reading error failure connects J24 of PCB controller and J3 of PCB
(can't read Preheater A/D preheater.
converter)
A/D Once the wire is checked, try to replace the PCB.
defective
10.3.11 DILUTER
Error ID Number Probable cause Possible Solution
and Description
(0501) Wiring failure Check pins in connector J23of PCB controller.
Diluter
Initialization Check the power supply connector (J6 on PCB Regulator). Un
error easy way of check this, is to visualize the LED on the
electronic board of diluter module (This is in case of having
the MODEL #2)
In case of having MODEL #1 check the fuse
Mechanical Check the belt below the Module.

failure
(0502) Contact your
Diluter invalid manufacturer.
command
(0503) Incorrect Volume Verify the aspiration Volume in the Method parameters.
Diluter invalid
operand
(0504) Contact your

Diluter invalid manufacturer.
command
sequence

10-30
(0505) Diluter not Execute initialization commands.
Diluter not initialized.
initialized
(0506) Mechanical Check possible obstacles in the movement of the syringe

Diluter Plunger failure plunger.
overload
Check the belt below the module.
(0507) Mechanical Check possible particles in the flow path to the valve.
Diluter Valve failure
overload
Check water quality. A better one should increase the
lifecycle
(0508) Wrong position of Check valve position, before moving the syringe.
Diluter Plunger the valve.
move not allowed
(0509) Contact your

Diluter command manufacturer.
overflow
10.4 Log File

Besides the above table, there’s an extra tool. This is the log file.
For example, in order to find out which module is producing a failure, we should go to the Log folder.
To access this folder we should go to Maintenance_Settings_Files and find the path in the Log file
folder.
Figure 10-2

10-31
Once the path has been found, minimize the program. Search the log file in Local Disk (the one in
that path). Once the folder has been found, search log.html files. These are identified by year,
month, and day (for example, the file corresponding to Tuesday, March 22nd, 2017 will have the
name 2017-03-22.log.html).
Figure 10-3

10-32
Once the file has been found, open it. We will have the following on display:
Figure 10-4

10-33
In order to locate an error message shown on the screen , it is convenient to start by the last
transactions on the Serial port. We can even check if the same error has been repeated in previous
transactions. The message informs us about the moment the error was produced, the process
number and the kind of error. All this information will enable us to find it on the screen.
We can also search for errors on the basis of autoanalyzer`s answer (Serial In) to computer (PC). This
search is done by Edition – Search this page (or else by ctrl+F). The number code or the complete
text of the command is entered and the searcher will start the search.
This file is a powerful tool for error search, as well as for an accurate diagnosis when facing
systematic or random failures.
All bidirectional messages for all the commands required by the computer (PC) and executed by
autoanalyzer, and all their responses, will be found in log.html.
Let us analyze the following message structure obtained from the one log.html file. The computer
(PC) sends a command to initialize vertical movement of autoanalyzer’s Robot. We will have the
following message structure in log.html:
(Example)
16:28:32.171 - Serial Out: probeInit A1
16:28:32.186 - Serial In: status A1 PC OK *#I-49,0 @00:12:15.808
16:28:32.639 - Serial In: probeCurPos A1 PC 0 *E0 iHl #O-49 @00:12:16.203
16:28:32.171 Serial Out is the command sent by the computer (PC) to autoanalyzer number one (A1)
to initialize the Robot’s vertical movement.
16:28:32.186 Serial In is the command sent by autoanalyzer number one (A1) to the computer (PC)
to be executed. Sending the following answer message, status OK without any comments *, #I-49
which is the order number given to entries by the controller’s meter. Finally, the time when the
command was executed.
16:28:32.639 asynchronous answer to the command required and executed by autoanalyzer.

Once the Robot’s vertical movement has been initialized, autoanalyzer sends the computer (PC) the
asynchronous answer to the required command. This is an indication that it has been positioned in
position 0 in relation to the Home sensor and there is no error E0. It is also showing the state of
sensors iHl; after that, the order number given to the exit by meter #O-49and, finally, the time the
command was executed.
Sensor state is very useful in order to establish a correlation with regard to mechanical movements.
The first letter, for example, tells us about the sensor, it shows abnormal movement of the Sample/
Reagent probe. If there was a collision, letter i becomes I (capital letter). By H it is indicating that the
Home sensor is activated. When it is deactivated, it becomes h. By l (small letter el) it indicates that
the lever detector is deactivated. When it is detecting liquid, it becomes L.

10-34
When one or several commands are queued and an error appears, these commands are eliminated
right away. Therefore, there is no answer for those commands. Let us look at the following log.html
of autoanalyzer’s starting sequence. The error message generates a Stop alarm and is immediately
shown by means of a message in the user’s interface.
Figure 10-5
Example:
3:01:27.500 - Serial Out: firmwareVersionsRead A1 2.09.06
13:01:27.546 - Serial Out: setQueue A1 0
13:01:27.578 - Serial In: queue A1 PC 0 *#O-3945 @02:00:34.910
13:01:27.593 - Serial Out: photoInit A1
13:01:27.640 - Serial Out: probeInit A1
13:01:27.656 - Serial In: amplifier A1 PC G75 71 *#O-3946 @02:00:34.982
13:01:27.687 - Serial Out: probeArmInit A1
13:01:27.718 - Serial In: status A1 PC QUEUED *#I-3948,0 @02:00:35.043
13:01:27.734 - Serial Out: probeGoFunnel A1
13:01:27.781 - Serial Out: srInit A1
13:01:27.828 - Serial Out: srGoReagentA A1 1
13:01:27.875 - Serial Out: syringeInit A1
13:01:27.921 - Serial Out: setWasherMode A1 Manual
13:01:27.968 - Serial In: washerMode A1 PC Manual *#O-15 @02:00:35.287
13:01:27.984 - Serial Out: washerInit A1
13:01:28.031 - Serial Out: valveSet A1 Probe
13:01:28.078 - Serial Out: reactionInit A1
13:01:28.093 - Serial In: probeCurPos A1 PC !error *P60 *E1 Ihl #O-3947 @02:00:35.412
13:01:28.109 - Serial In: errorMessage A1 PC 0101 @02:00:35.427

10-35
According to the log.html the probe movement showed an error (see text in blue letters).
When we analyzes the log file, we find computer sent a command to initialize the probeArm:
“13:01:27.640 - Serial Out: probeInit A1”
It continues executing the other required commands:
13:01:27.687 - Serial Out: probeArmInit A1
Analyzer recivied the synchronous answer “QUEUED”, until it receives the asynchronous answer with
error 13:01:28.093 - Serial In: probeCurPos A1 PC !error *P60 *E1 Ihl #O-3947 @02:00:35.412,
corresponding at "probeInit" command required by the computer (PC). We find *E1 Ihl, the status of
“I” corresponding at impac of probe in Vertical.
Detector Status and level sensor table
Module Command Status Description

Robot Vertical Position probeInit iHl Initializing vertical Mov.
Robot Horizontal Position probeArmInit iH Initializing horizontal Mov.
Reaction Tray ReactionInit ihw Initializing Reaction Tray
Sample-Reagent Tray srInit iH Initializing S-R Tray
Photometer Filter Wheel photoInit H Filter position synchronism
Washer Initialize Washer washerInit Ud Initializing washer
Activated Deact. Comment

Probe Impact I i I: probe impact, Stop alarm
Starting Position H h H: detected starting position
S/R Sensor L l L: detected sample and/or reagent level
Cuvette Washing W w W: the washer is not up
Filter Position H h H: filter position synchronism
Washer position U u U: washer outside cuvettes
Washer position down D d D: cuvette washer down
Let us analyze the following failure message:
Figure 10-6
We search the log.html for possible failures.

10-36
Figure 10-7
By looking at log.html we can notice that one of the possible failures is the Robot. When we initialize
the arm we get the !error answer. The comment is *P300 *E1 and the sensor state indicates that
impact sensor, IHl is activated.
When there is no error, the letter is E=0. Letter P indicates the number of steps the mechanism has
performed. The following is an error list that may appear in autoanalyzer’s responses and their
meaning:
E1: movement cannot be initialized.

E2: probe impact
E3: home sensor not found
E4: movement verification error
E5: checking sensor not found
E6: level sensor does not activate
E7: washer is not up
E8: stopped due to lack of movement safety conditions
E9: Incorrect Probe Level Detection
E10: Temperature reading error
“debug” messages may appear which require additional information. They may be sent either from
the computer (PC) or from autoanalyzer

10-37
It is important to bear in mind the asynchronous answer table (see Section 11.4.1 of the Technical
Service Manual) to be able to interpret the responses sent by autoanalyzer to the computer (PC).
More examples of errors that can be found in the log file
Example 1
08:19:57.954 - Serial Out: reactionGoPhoto A1 19
08:19:58.014 - Serial Out: photoSetFilter A1 340
08:19:58.074 - Serial Out: photoRead A1 *[ALT][ALT]
08:19:58.074 - Serial In: photoCurVal A1 PC 340 *P364 *E0 H #O-5028 @15:45:01.496
08:19:58.234 - Serial In: reactionCurPos A1 PC 218 *E0 ihw #O-5027 @15:45:01.577
08:19:58.234 - Serial In: reactionCurDispense A1 PC 0 @15:45:01.590
08:19:58.234 - Serial In: reactionCurPhoto A1 PC 19 @15:45:01.603
08:19:58.737 - Serial In: photoCurRead A1 PC !error *UNCOVERED *Re 4112770 4306277 C019 F340
#O-5029 @15:45:02.116
Error - type: Hardware Error
(0322) Reaction tray uncovered. Please place the lid back on
Last transactions through serial port
08:19:59.817 - Serial In: syringeCurPos A1 PC 0.000 *` #O-5018 @15:45:03.204
08:19:59.937 - Serial In: valveCurVal A1 PC ByPass *` #O-5019 @15:45:03.331
08:20:01.637 - Serial In: pumpRelMove A1 PC 1400 #O-5020 @15:45:05.013
08:20:01.762 - Serial In: valveCurVal A1 PC Probe *` #O-5021 @15:45:05.140
08:20:02.491 - Serial In: probeArmCurPos A1 PC 270 *E0 ih #O-5023 @15:45:05.902
08:20:02.511 - Serial In: probeArmCurVal A1 PC ReagentA @15:45:05.915
Error - type: Critical (it will retry)
Error while processing ALT process number: 150624172548654 Processing error
In this case we can see the following information: in the answer (photoCurRead #O-5029) to the
command photoRead (#I-5029) you can see the message !error *UNCOVERED* and then the text
errorMessage A1 PC 0322. Here we can see that the error appeared at the time a reading was taking
place (photoCurRead cuvette 19 and filter340) and you can see that it appears to be that the reaction
tray cover wasn’t placed correctly: *UNCOVERED*, finally it throws the error message number: 0322,
using this number you can search it in the error messages chart and find the possible causes and
solutions to this error.

10-38
Example 2
08:25:02.839 - Serial Out: probeGoSample A1 7

08:25:02.886 - Serial Out: probeIn A1 *[ALT][ALT108 sem][S]
08:25:02.932 - Serial Out: syringeAspirate A1 20 500
08:25:02.960 - Serial In: probeArmCurPos A1 PC 0 *E0 iH #O-6344 @15:50:06.411
08:25:02.980 - Serial In: probeArmCurVal A1 PC Funnel @15:50:06.424
08:25:03.020 - Serial Out: probeOut A1
08:25:03.220 - Serial In: probeCurPos A1 PC 175 *E0 ihL #O-6345 @15:50:06.619
08:25:03.340 - Serial In: srCurPos A1 PC 477 *E0 iH #O-6341 @15:50:06.687
08:25:03.340 - Serial In: srCurVal A1 PC Sample 7 @15:50:06.700
08:25:03.700 - Serial In: photoCurRead A1 PC 0.0093 *Rr 3992278 4307631 C046 F340 #O-6339
@15:50:07.100
08:25:03.790 - Serial Out: debugMessage A1 ALT108 sem Piridoxal-LABT - 150624172548654/36 - 46
- alog: CinÃ©tica Fin Lectura Muestra Extra
08:25:03.920 - Serial In: probeArmCurPos A1 PC 160 *E0 ih #O-6348 @15:50:07.339
08:25:03.920 - Serial In: probeArmCurVal A1 PC Sample @15:50:07.352
08:25:03.920 - Serial In: syringeCurPos A1 PC 204.000 *` #O-6347 @15:50:07.377
08:25:04.190 - Serial In: probeCurPos A1 PC !error *P113 *E2 Ihl #O-6349 @15:50:07.644
Error - type: Hardware Error
(0101) Probe Impact (Vertical movement)

10-39
Here you can see how the command probeGoSample is succesfully executed (probeArmCurPos step
160 and probeCurVal indicating the probe arm is on Sample position) and then the attemp to
perform the command probeIn an impact on step 113 appears (the robot arm went down 113
steps), this is demonstrated by the appearance of the text E2 (probe impact) and by the status of the
impact sensor (Ihl) then you can see the error message number 0101. You could verify where the
impact took place and if it’s possible that it wasn’t an actual impact, by using the information given in
the log file and the commands console. For example if there’s a vertical impact at a height that
normally there wasn’t an obstacle for the vertical movement, then it might be a false impact due to a
different kind of hardware failure (this should always be considered among with the user’s story and
events description)
How to reproduce an impact error using the log file and the commands console:
Using this case as an example, we know the impact was during the vertical movement while going in
a sample, so we should look for the sample it was going into: as we can see in the yellow highlighted
text the sample that it was going to is simple 17, so the first command to use would be for the SR
tray to go to sample 17 (srGoSample 17) the you would have to move the probe arm to the sample
position (probeGoSample) and finally move in as many steps as shown in the log file where the error
occured (P113 in the green highlighted part) (probeMoveIn 113) once this is done you can check if
the probe impact might have been against a sample tube cap for example.
Example 3
08:27:41.920 - Serial Out: probeOut A1

08:27:41.936 - Serial In: residuesVessel A1 PC 29% *CR-9106 *E0 #O-6459 @15:52:45.404
08:27:41.952 - Serial In: status A1 PC QUE,0 @15:52:45.423
08:27:41.998 - Serial In: concentratedResiduesVessel A1 PC 27% *CC-3359 *E0 #O-6459
@15:52:45.458
08:27:42.030 - Serial In: probeCurPos A1 PC 0 *E0 iHL #O-6461 @15:52:45.489
08:27:44.218 - Serial In: reactionCurPos A1 PC 1595 *E0 iHw #O-0 @15:52:47.675
*…+
08:28:39.271 - Serial In: reactionCurPos A1 PC 635 *E0 iHw5:53:42.660
Error - type: Critical (it won’t retry)
Error while processing ALT process number: 150624172548654 Unknown error! The method must be
repeated
In this case the error is not as clear as in the previous example, since there’s no ¡error text or error
message number, you can only see the Error processing and Unknown error. The Unknown error is
due to a lost of characters in the status. As you can see in this example the status for the command
probeOut is status A1 PC QUE,0 when it should say status A1 PC QUEUED.

10-40
Other ways that the loss of characters is shown is by message Error processing alone or with
messages of the type: Error:probeCurPos o Error:washerCurVal:

11:45:07.901 - Serial In: reactionCurDispe:18:47.689
*…+
Error:reactionCurDispense
Notice the yellow highlighted line: there the text should say, for example: reactionCurDispense A1
PC x @00:18:47:689, the characters in red are missing. And the error showing is:
Error:reactionCurDispense.

13:21:11.968 - Serial In: reactionCurDispense A1 PC 0 @0reactionCurPhoto A1 PC 0 @01:54:52.495
*…+
13:23:31.500 - Serial In: reactionCurPos A1 PC 1585 *E0 iHw #O-0 @01:57:12.023
Error while processing ALT process number: 150625113001317 Processing error
This is a similar case where the error is shown as Error while processing– Processing error.
Example 4:
09:23:57.634 - Serial In: photoCurRead A1 PC 0.0070 *Ri 4564841 4111844 C011 F578 #O-1417
@00:12:43.574
09:23:57.634 - Serial In: srCurPos A1 PC 284 *E0 iH #O-1422 @00:12:43.599
09:23:57.634 - Serial In: srCurVal A1 PC ReagentA 1 @00:12:43.611

10-41
09:23:57.837 - Serial Out: debugMessage A1 Ca - 150625091230177/2 - 11 - alog: EPRB Fin
Error - type: Not critical
Processing error - Ca processo: 150625091230177 The result can’t be saved in the database.
Last transactions through serial port
Error - type: Not critical

insert into pfbr (proc,id,name,info,con1,con2,abs1,abs2,redil,bicro,blanco,limup,limdown,unit,cuv)
values ('150625091230177',2,'Ca','',nan,0,-0.0019,0,0,0,0,0,0,'mg/dL',11)
Here you can see a reaction that’s been completed (EPRB Fin: End Point Reagent Blank ended) but
the result obtained can not be saved in the database, and you can see that the filed corresponding to
the concentration says “nan”. The result is not saved in the database since is not a numeric value
(nan= not a number), this is due to a lack of calibration of the method or lack of asigning a factorm
and it’s shown with an error message on screen and also the error is saved in the log file.

10-42
11 VALIDATIONS
By clicking on Maintenance and selecting Validations, some validation tests are displayed which are
used to control how the components of the instrument are working (Figure 12-24). The
spectrophotometer, the diluter and the washer are controlled through these tests. Each user can
create an internal quality control program of their Autoanalyzer by using these tests.
Warning: A Special Washing cycle with Sodium Hydroxide 0.2 N is recommended before making
the Validation Tests (see Chapter 7 point: 7.7)
Figure 11-1
11.1 Stray Light
Stray light is any electromagnetic radiation of a different wavelength from that selected wavelength
reached by the detector and which is registered by the instrument.
This test is based on the absorbance measurement of a Sodium Nitrite solution. The solutions of this
substance absorb all wavelength radiation lower than 390 nm, that is why they are called optically
opaque to UV light.
Necessary materials:
Sodium Nitrite Solution 50 g/l

Automatic pipette
Procedure:

11-1
1. Dispense manually 300 µl of the solution in cuvette 1 or in any cuvette, indicating it on the
screen (Figure 12-25)
2. Wait for 5 minutes to reach thermal stability of the solution in the cuvette
3. Select Maintenance  Validations  Stray Light Cuvette number  OK  Filter  OK
4. The instrument reads at the selected wavelenght, informing the value in absorbance units
AU.
5. Perform the reading in duplicate.
Acceptable stray light: Abs. Higher tan 3AU
Figure 11-2
Figure 11-3
11.2 Photometer Precision
Photometric precision is the measurement of the dispersion of absorbance measurements around

the mean and it is expressed as a variation coefficient. This measurement is always made in the same
cuvette.
Copper Sulfate Solution 30 g/l in Sulfuric Acid 0.45 N

Automatic Pipette
Procedure:
1. Dispense manually 300 µl of the solution in cuvette 2 or in any cuvette, indicating it on the
screen (Figure 12- 27)
2. Wait for 5 minutes to reach thermal stability of the solution in the cuvette
3. Select Maintenance  Validations  Photometer Precision  Cuvette Number  OK 
Filter  OK

11-2
4. The instrument makes 30 correlative readings of the solution at the selected wavelenght,
reporting the obtained values in absorbance units AU, the arithmetic mean and the
coefficient of variation CV% for this data.
5. Perform the test in duplicate.
Acceptable photometric precision: CV% lower than 1.0%
Figure 11-4
Figure 11-5
11.3 Photometric Accuracy
Photometric Accuracy means similarity between the absorbance unit and the real absorbance of a
specific certified solution measured using reference standards.
The error while reading the absorbance of this solution regarding the certified value is called
photometric inaccuracy.
2 solutions of Potassium Dichromate in Perchloric Acid 0.001 N of different concentration and with
known and certified absorbance, measured using NIST Reference Standards Certificates (National
Institute of Standards and Technology). Example: solution A and B
Automatic pippete
Procedure:
1. Dispense manually 300 µl of the solution A in cuvette 3 or in any cuvette, indicating it on the

11-3
2. Dispense manually 300 µl of the solution B in cuvette 4 or in any cuvette, indicating it on the
3. Wait for 5 minutes to reach thermal stability of the solutions in the cuvette
4. Select Maintenance  Validations  Photometric Accuracy  Cuvette number  OK
 Cuvette Number  OK  Filter  OK
5. The instrument makes the readings of both solutions at the selected wavelenght, reporting
the value in absorbance units AU.
6. Make the reading duplicate.
7. Calculate the % of photometric inaccuracy according to the following formula:
% Photometric inaccuracy=[measured abs – reference abs] x 100

Reference abs.
Acceptable photometric inaccuracy: lower than 5.0%
Figure 11-6
Figure 11-7
Figure 11-8

11-4
11.4 Photometric Linearity
Photometric linearity means the photometric capacity to make absorbance readings proportional to
concentration changes for solutions of increasing concentrations of a substance which follows Beer’s
law.
Potassium Dichromate solutions in Perchloric Acid 0.001 N of increasing concentrations and known
and certified absorbance measured using NIST Reference Standards Certificates (National Institute of
Standards and Technology). Example: 25, 50, 100 and 200 mg/l (solutions A, B, C and D respectively).
Automatic Pipette.
Procedure:
1. Dispense manually 300 µl of solution A in cuvette 5 or in any cuvette, indicating it on screen

(Figure 12-32)
2. Dispense manually 300 µl of solution B in cuvette 6 or in any cuvette, indicating it on screen
(Figure 12-33)
3. Dispense manually 300 µl of solution C in cuvette 7 or in any cuvette, indicating it on screen
(Figure 12-34)
4. Dispense manually 300 µl of solution D in cuvette 6 or in any cuvette, indicating it on screen
(Figure 12-35)
5. Wait for 5 minutes to reach thermal stability of the solutions in the cuvette
6. Select Maintenance  Validations  Photometric Linearity  Cuvette Number  OK 
Cuvette Number  OK  Cuvette Number  OK Cuvette Number  OK Filter  OK
7. The instrument makes the reading of four solutions at the selected wavelenght, informing
the values in absorbance units AU.
8. Perform the test in duplicate.
9. With the results obtained, make a graph of absorbances found (y) in relation to reference
absorbances (x) and calculate the correlation coefficient by linear regression.
Acceptable photometric linearity: higher tha 0.9995
Figure 11-9

11-5
Figure 11-10
Figure 11-11
Figure 11-12
Figure 11-13
11.5 Diluter Precision
This test allows to determine volume precision of the diluter hydraulic system by making repeated
dilutions and readings of the same reaction at 340 nm.
Sample solution: Potassium Dichromate solution 5g/l in Perchloric Acid 0.001 N

Reagent solution: Potassium Dichromate solution 0.25 g/l in Washing Solution (Triton X-100 diluted).
It is prepared by diluting 5 ml of Sample Solution in one liter of Washing Solution.
Procedure:
1. Set a new method with the parameters shown in Figure 12-38 Methods  Settings

11-6
3. Place the Sample Solution in a sample tube and the Reagent Solution in a reagent bottle and assign
a position in the reagents tray.
4. Make the Diluter Precision test Maintenance  Validations  Diluter Precision  select the
method Dichromate Test  assign a position to the Sample Solution: 1 define the number of
replicates to be made, minimum 10  OK
Make the test duplicate.
Acceptable diluter precision: CV% lower than 1.5%
Note: this test can also be used to validate methods in use, determining each test CV% at different
concentration values.
Figure 11-14
ADAPTATION
GENERAL SPECIALS
Name Dichromate Time for Reagent blank 60
Type End Point R.Blank Interval between blanks 72
Main Wavelength 340 Incubation Time 60
Bicrom. Wavelength 700 Repetition 0,4
Units -- Linear Limit 2,0
Decimals 4
Sample Vol. 6 ADVANCED
R1 Vol. 300 Initial Air Gap 2
R2 Vol. 0 Initial gap Speed 500
Time to Disp R2 0 Gap Reagent/Sample 2
Min. Abs 0 Reagent/Sample Gap Speed 500
Max. Abs 2 R1+ Sample Dispensing Speed 2500
Verification time 16 R2 Dispensing Speed 2500
FACTOR R1 Aspiration Speed 2000
Decreasing method No R2 Aspiration Speed 2000
Factor Yes/ 1 Dilution with Sample

11-7
Calibrator No Minimum sample volume 2
Interpolation Linear Sample aspiration Speed 500
Figure 11-15

11-8
12 DIAGRAMS
12.1 Identifying printed circuits
The Printed Circuit will be identified by a fantasy name (related with its function) followed by letter r
and the revision number, which will be made up of 2 parts: the main revision indicator and the
secondary revision indicator, separated by a full stop.
The main revision indicator will be modified when a modification affecting its function, size, etc is
introduced to the printing, representing an important change in its functions or geometry.
The secondary revision indicator will be modified when a minor change, like a change of its
components, footprints, component reordering, etc, is introduced to the printing.
The resulting format will be NAME rX.Y.
The file related with the printed circuit will have the format NAME rXY.pcb
The electrical scheme corresponding to the printed circuit will have, besides, an electrical scheme
revision indicator which will be modified with the changes in the scheme not involving changes in the
printed circuit, for example changes in the value of its components. Revision format will be X.Y.Z, and
the file name will be NAME rXYZ.dsn.

12-1
12.2 Electrical and electronic scheme

12-2
12-3
12-4
12-5
12-6
12-7
12-8
12-9
12-10
12-11
12-12
12-13
12-14
12-15
12-16
12-17
12-18
12-19
12-20
12-21
12-22
12-23
12-24
12-25
12-26
12-27
12-28
12-29
12-30
12-31
12-32
12-33
12.3 Mechanical scheme
12.3.1 ROBOT HEADER MODULE

12.3.2 ROBOT MODULE
12.3.3 CUVETTES TRAY MODULE

12.3.4 PHOTOMETER MODULE
SERVICE MANUAL
InCCA Bit v160101
Torcuato de Alvear 46
B1878DMB - Quilmes - Buenos Aires
Argentina
Tel/Fax +54 11 4252 - 2626
www.diconex.com

InCCA Bit Service Manual SM160101

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SERVICE MANUAL

InCCA Bit Service Manual - MSv160101

1.1 TRANSPORTATION AND STORAGE ...................................................................... 1-1

2 INSTALLATION AND CONNECTIONS OF THE ANALYZER ... 2-1

2.1 ELECTRICAL REQUIREMENT .............................................................................. 2-1

3 ANALYZER TECHNICAL SPECIFICATIONS .......................... 3-1

3.1 GENERAL SPECIFICATIONS ............................................................................... 3-1

4 GENERAL FUNCTIONS ..................................................... 4-1

InCCA Bit Service Manual - MSv160101

5 BASIC OPERATIONS ......................................................... 5-1

5.1 USING COMMANDS CONSOLE .......................................................................... 5-1

6 DIRECTIONS FOR ASSEMBLING AND DISASSEMBLING ..... 6-1

6.1 DISASSEMBLING THE CABINET........................................................................... 6-1

7 MODULES ADJUSTEMENT ............................................... 7-1

7.1 REACTION TRAY DISPENSING OFFSET CALIBRATION ............................................... 7-1

InCCA Bit Service Manual - MSv160101

8 PARTS REPLACEMENTS ................................................... 8-1

8.1 LIGHT BULB REPLACEMENT .............................................................................. 8-1

9 MAINTENANCE PROGRAM .............................................. 9-1

9.1 DAILY MAINTENANCE .................................................................................... 9-1

InCCA Bit Service Manual - MSv160101

10 TROUBLESHOOTING .................................................... 10-1

10.1 CHEMICAL PROBLEMS ................................................................................ 10-1

11 VALIDATIONS .............................................................. 11-1

11.1 STRAY LIGHT............................................................................................ 11-1

12 DIAGRAMS .................................................................. 12-1

InCCA Bit Service Manual - MSv160101

InCCA Bit Service Manual - MSv160101

InCCA Bit Service Manual - MSv160101

Keep lids closed when the instrument is working.

Do not look straight at barcode detector light beams.

InCCA Bit Service Manual - MSv160101

The use of controls, as indicated by good laboratory practices, is also recommended.

Carry out the maintenance plan as indicated in this manual.

Read all warning messages.

Symbols used in the manual and the instrument

Consult instructions for use

“In vitro” diagnostics device

InCCA Bit Service Manual - MSv160101

InCCA Bit Service Manual - MSv160101

1.1 Transportation and storage

Do not pile up more than four wooden boxes

Keep dry, protect from humidity and rain

Handle with care

InCCA Bit Service Manual - MSv160101

NOTE: Instrument remains fastened to the bottom.

InCCA Bit Service Manual - MSv160101

Unscrew the four screws located on the sides.

Screws are placed this way:

InCCA Bit Service Manual - MSv160101

Installation must be carried out by trained personnel.

2.1 Electrical requirement

Width: 80 cm, Height: 47 cm, Depth: 58 cm

2.3 Environmental requirements

2.4 Moving the instrument

2.5 PC minimum requirements

InCCA Bit Service Manual - MSv160101

The instrument must not be subject to vibrations or sudden changes of temperature.

Avoid direct sun light or illumination on the instrument.

Warning: The instrument must be connected to a PC which meets the requirements

InCCA Bit Service Manual - MSv160101

Switch On/OFF RS 232 Serial Port

1. Connect the interlock to 110-220 V, 50-60 Hz supply.

3. Connect the RS-232 wire to the PC before switching on the instrument.