Extraction, characterization

and utilization of dietary

fiber from mango peel
 Colorectal cancer in the USA
 According to American
cancer society:
✓By 2017, an estimated
95,520 new cases of
colon cancer and
39,910 cases of rectal
cancer
✓Almost 40% will die.
✓One of the reason of
colorectal cancer
lack of dietary fiber in
the diet. https://www.foodexecutive.com/en/marketing/1060-canned-preserved-food-in-the-usa.html
Colorectal cancer in the Philippines
 According to the
Philippine Society of
Gastroenterology
(PSG), colorectal
cancer is the most
prevalent cancer in the
GI.
 Annually, there are
3000 new cases and
2,000 dies
 Doctors recommend a
high fiber diet to
reduce the risk of the
cancer
https://abmachinesguide.com/8-easy-steps-to-improve-your-nutrition/
Prevalence of diabetes in the Philippines

 1 out of 5
Filipino is
diabetic
 At 2030, over 7
million Filipino
will be diabetic
 Dietary fiber
helps to lower
sugar in the
blood.
 Dietary Fiber
It is the
plant part
that is
resistant to
digestive
enzymes.

Hemicellulose, chitosan, lignin, chitin,

Hemicellulose, chitosan, beta-glucan,resistant starch, cellulose, beta-glucan
fructan, pectin, gum, mucilages

http://www.thewellnesssite.org/important-dietary-fiber-pros-cons/
 Role of dietary fiber in human health

https://barndadnutrition.com/dietary-fiber-health/
Sources of Dietary Fiber

http://www.closertothecure.com/dietary-fiber-facts/
Potential of Mango Peel as Source of
dietary fiber
Chemical composition of mango peel
Objectives
❑ Optimize the hot water treatment of fiber using
Response Surface Methodology
❑ Characterize the dietary fibers obtained from the
optimum condition
❑ Standardize and validate the optimized method
❑ Compare the dietary fibers with that of obtained
through enzymatic method
❑ Apply the dietary fibers as encapsulation agent and
bakery ingredient.
Methodology
Drying and
Collection of Hot Water
grinding of mango
Mango peel Treatment
peel

Dietary fiber
Characterization Freeze- drying
concentrate

Utilization
Methodology

Heat at 100OC, Wash at 100OC

Centrifugation
5 minutes for 5 times

Hot water treatment

Methodology
Mango Peel
waste powder

Hot water
treatment

Dietary fiber Dietary fiber

High sugar extract extract (High in concentrate (High
soluble DF) in insoluble DF)
Characterization
 Proximate analysis
 Fiber analysis: Total, Soluble, Insoluble, Klaso lignin, Uronic
acid, neutral sugars
 Sugar profile analysis: Rhaminose, Arabinose, Xylose,
Mannose, Glucose, Galactose
 Polyphenolic compounds: Flavoinoids, Flavonols, Acids
 Water holding capacity
 Oil holding capacity
 Swelling capacity
 Cation holding capacity
 In vitro test: (GDRI)
HPLC analysis of polyphenolic
compounds & sugars
 Polyphenolic compounds
Reversed phase, C-18 Column
UV detector

 Sugars
RID detector, Amine based column

https://americanlaboratorytrading.com/lab-equipment-products/shimadzu-rid-10a-refractive-index-detector_9031
https://www.shimadzu.com/an/hplc/prominence/uflc2.html
Utilization
Dietary fiber concentrate (High in insoluble dietary
fiber): Bakery ingredient for cupcake & cookies
Dietary fiber extract (High
in soluble dietary fiber, i.e
pectin): Additive to food &
beverage, encapsulating
High sugar extract:
encapsulating
Tentative goals
0 – 3rd months Procurement of chemical
and supplies, optimization of hot
water treatment
4 – 6th months characterization, comparisons and
downstream processing
7-9th month Storage stability and utilization
10-12th month Storage stability,
terminal report
Collaboration


Budget (P1,000,000)
Honorarium (6,000/month) 72, 000
 Travel 20, 000
Communication 8, 000
Supplies 600, 000
Other Services 300, 000
Administrative cost (10%) 100, 000
Thank you!